##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512967_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2501616
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.250410134888806	31.0	31.0	34.0	26.0	34.0
2	30.405743327513097	31.0	31.0	34.0	26.0	34.0
3	30.50247360106427	31.0	31.0	34.0	27.0	34.0
4	33.94914007585497	37.0	35.0	37.0	30.0	37.0
5	33.56672966594393	37.0	35.0	37.0	28.0	37.0
6	33.41621855632519	37.0	35.0	37.0	28.0	37.0
7	33.272920384263614	37.0	35.0	37.0	27.0	37.0
8	33.152644930317045	37.0	35.0	37.0	26.0	37.0
9	34.58943339025654	39.0	35.0	39.0	27.0	39.0
10	34.47109788232886	39.0	35.0	39.0	27.0	39.0
11	34.49357375392546	39.0	35.0	39.0	27.0	39.0
12	34.346095883620826	39.0	35.0	39.0	26.0	39.0
13	34.32371674949313	39.0	35.0	39.0	26.0	39.0
14	35.356902897966755	40.0	36.0	41.0	25.0	41.0
15	35.37503117984535	40.0	36.0	41.0	25.0	41.0
16	35.35057858600201	40.0	36.0	41.0	25.0	41.0
17	35.32370915440259	40.0	36.0	41.0	25.0	41.0
18	35.24775665010137	40.0	36.0	41.0	25.0	41.0
19	35.23710273679094	40.0	36.0	41.0	25.0	41.0
20	35.20717208396493	40.0	36.0	41.0	25.0	41.0
21	35.15021809902079	40.0	36.0	41.0	24.0	41.0
22	35.10698564447941	40.0	36.0	41.0	24.0	41.0
23	34.99668214466169	40.0	35.0	41.0	23.0	41.0
24	34.97962357132349	40.0	36.0	41.0	23.0	41.0
25	34.88677239032689	40.0	35.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7736496026786881
1101	2	0.8201869734663916
1101	3	0.8502303803048328
1101	4	0.5617012157269841
1101	5	0.8627112397924463
1101	6	0.8434984427256609
1101	7	0.9360686650865802
1101	8	0.8649213921392231
1101	9	0.7487649920638972
1101	10	1.0522228869821575
1101	11	1.0857538033042218
1101	12	1.0749026646694446
1101	13	1.1371054787593735
1101	14	1.1597246987606198
1101	15	1.0695810378955883
1101	16	1.2374260913608452
1101	17	1.2735361263134592
1101	18	0.6927444923214523
1101	19	1.032458311019802
1101	20	1.0739770210271615
1101	21	1.2271294202456104
1101	22	1.22689309290476
1101	23	1.1585190830444816
1101	24	1.0691882511151434
1101	25	1.1403491851218561
1102	1	0.7716267365862848
1102	2	0.7632412151787378
1102	3	0.6909032072429682
1102	4	0.7306680651135693
1102	5	0.9373167213899407
1102	6	0.8701375447877879
1102	7	0.9539202965144042
1102	8	0.9564481997612475
1102	9	1.1359620685452327
1102	10	1.1231067293209733
1102	11	1.1122356618268014
1102	12	1.0688559927822041
1102	13	1.2969933409363534
1102	14	1.444550383960312
1102	15	1.4303672555933176
1102	16	1.2567958642660884
1102	17	1.2844684155329844
1102	18	1.3617368180849567
1102	19	1.3921995796180795
1102	20	1.4239587490355134
1102	21	1.4667545833883935
1102	22	1.4003546434137633
1102	23	1.487160377745603
1102	24	1.3723408483482444
1102	25	1.3149258491782376
1103	1	0.5742951982611224
1103	2	0.4598040477060259
1103	3	0.4961490760713332
1103	4	0.5355962109062347
1103	5	0.537172664983089
1103	6	0.6932592981906467
1103	7	0.6313438338130268
1103	8	0.6306614920603337
1103	9	0.754000157284338
1103	10	0.8574366655779997
1103	11	0.8316235552544384
1103	12	0.847974169621672
1103	13	0.8793690952025344
1103	14	0.9251660871382228
1103	15	0.6228028803007035
1103	16	0.7785969564673394
1103	17	0.953489005619268
1103	18	1.0110611195455732
1103	19	0.9651590911056971
1103	20	0.9963558447628031
1103	21	0.6869555224456292
1103	22	0.9182873255281265
1103	23	0.9404953473971815
1103	24	1.0134363359604421
1103	25	1.028045284692368
1104	1	0.6164917298945376
1104	2	0.665770428454227
1104	3	0.5733444574685436
1104	4	0.5340001382156885
1104	5	0.5318044618988083
1104	6	0.6757976432817969
1104	7	0.7007621652603717
1104	8	0.7083925078847884
1104	9	0.904564088344749
1104	10	0.9445419693596335
1104	11	0.9725052805374546
1104	12	0.8417599465603018
1104	13	0.9502326250923616
1104	14	1.1775878895843945
1104	15	1.200420106369691
1104	16	1.199416080933311
1104	17	1.1050923306930187
1104	18	1.1161415030965642
1104	19	1.160654209525191
1104	20	1.3058192177598684
1104	21	1.1515534730784225
1104	22	1.2158852374998261
1104	23	1.0819190135276244
1104	24	1.116136182988626
1104	25	1.2437396613123326
1105	1	0.6545196816193553
1105	2	0.6972263419410538
1105	3	0.6859775664520171
1105	4	0.676777355273714
1105	5	0.6452707508696065
1105	6	0.7408869835965533
1105	7	0.8649464853055733
1105	8	0.9357200679731861
1105	9	0.9984680732963938
1105	10	0.9929542340664455
1105	11	1.005612219397932
1105	12	0.9455821655607082
1105	13	1.0377769327955377
1105	14	1.078340028528892
1105	15	0.9655637218782687
1105	16	1.1443207183025308
1105	17	0.8784933964942567
1105	18	1.167036114424839
1105	19	1.124159253214863
1105	20	1.1815043956133593
1105	21	1.0048905610655794
1105	22	1.087547638359304
1105	23	1.0758762513663527
1105	24	1.1351162368548984
1105	25	1.1617432263747176
1106	1	0.5842422516013741
1106	2	0.5349844386121454
1106	3	0.5294881749816369
1106	4	0.5600890484266046
1106	5	0.698526702403349
1106	6	0.5756069344873893
1106	7	0.5895571314997596
1106	8	0.6057893262330083
1106	9	0.6490032683630105
1106	10	0.8270822025715105
1106	11	0.7044965155982652
1106	12	0.8696666697391535
1106	13	0.6938578234995489
1106	14	0.8509500473030798
1106	15	0.9043947261085137
1106	16	0.9042276122733881
1106	17	0.8103553912057251
1106	18	0.9676271285698661
1106	19	0.995205079080641
1106	20	0.9728616851589749
1106	21	0.9220292935029022
1106	22	0.9841716733863848
1106	23	1.016150515237129
1106	24	0.8899416097970203
1106	25	1.0220485514082895
1107	1	0.4058374814665662
1107	2	0.46496677144618914
1107	3	0.37710310757756105
1107	4	0.47203454906031794
1107	5	0.40659406807527887
1107	6	0.5003428871701061
1107	7	0.6090323014502133
1107	8	0.571606240624071
1107	9	0.4922801435790518
1107	10	0.6279703617296306
1107	11	0.6348245103749335
1107	12	0.4948097353765135
1107	13	0.5568691151230141
1107	14	0.7743590421949662
1107	15	0.692692149006696
1107	16	0.580733162067915
1107	17	0.6062775404548759
1107	18	0.6839404519174082
1107	19	0.8144805332420262
1107	20	0.7633204553705966
1107	21	0.7391597232759182
1107	22	0.828456729268396
1107	23	0.7953291840545802
1107	24	0.8936872410141348
1107	25	0.9266400942127646
1108	1	0.4167792641238641
1108	2	0.4025015431143615
1108	3	0.41392840961256283
1108	4	0.45102646416896164
1108	5	0.4621667223594059
1108	6	0.3815595510295182
1108	7	0.4525785522349679
1108	8	0.5219465563635737
1108	9	0.4099581872803384
1108	10	0.6166513862241985
1108	11	0.5401422149599426
1108	12	0.6902892909116503
1108	13	0.6881953894886976
1108	14	0.7089944309486
1108	15	0.7046061347458448
1108	16	0.5913171474497787
1108	17	0.6032149540042084
1108	18	0.5914406688415781
1108	19	0.6058215584511686
1108	20	0.6079268273266365
1108	21	0.7335481502439265
1108	22	0.6818943393255097
1108	23	0.7626406259112457
1108	24	0.7784176935945126
1108	25	0.6193261768685261
1109	1	0.27723310052733297
1109	2	0.28955088133781715
1109	3	0.26495313403806264
1109	4	0.45022361511954756
1109	5	0.39237097150594025
1109	6	0.13799158370262887
1109	7	0.31996681745307143
1109	8	0.49985513043332475
1109	9	0.5491679280294761
1109	10	0.46368226435497206
1109	11	0.498432112593008
1109	12	0.45625908076663535
1109	13	0.4763963924521093
1109	14	0.5529697506462199
1109	15	0.6252914909454503
1109	16	0.43912631915127776
1109	17	0.6261338484091326
1109	18	0.602818296421674
1109	19	0.8201942765981372
1109	20	0.7508638851122029
1109	21	0.8128251934675816
1109	22	0.8103796026491281
1109	23	0.7814805611512909
1109	24	0.3469735206671558
1109	25	0.5450738624187395
1110	1	0.18752543725766202
1110	2	0.1539077507459119
1110	3	0.22130521837159733
1110	4	0.2911800398425797
1110	5	0.20185384068931
1110	6	0.1495456774042836
1110	7	0.11241704575424194
1110	8	0.3239559711128095
1110	9	0.4119159636633043
1110	10	0.41885567223377507
1110	11	0.24775878586525835
1110	12	0.4723587683067123
1110	13	0.130782010896894
1110	14	0.3222550425573374
1110	15	0.4118750089967307
1110	16	0.4374675462686639
1110	17	0.2874649753418623
1110	18	0.1961681096607606
1110	19	0.4122757499834293
1110	20	0.4215903742907372
1110	21	0.3975270286163166
1110	22	0.24139553435966832
1110	23	0.3422388638117937
1110	24	0.16433882410966305
1110	25	0.29893931965099796
1111	1	-0.2187740482379148
1111	2	-0.1209681001199776
1111	3	-0.1492788373558973
1111	4	-0.20883945042328378
1111	5	0.1561707802004051
1111	6	-0.10804423736728808
1111	7	0.13095035886401263
1111	8	0.20365973450947195
1111	9	-0.23031685663220003
1111	10	-0.13250523075603127
1111	11	0.09874528277750017
1111	12	-0.02254076419706763
1111	13	0.11582564400595174
1111	14	0.29036182760131624
1111	15	0.3696264807047811
1111	16	0.1351551902505932
1111	17	0.404807182342374
1111	18	0.18433674638806252
1111	19	0.36252038176599655
1111	20	0.24612780853038885
1111	21	0.1413902000029026
1111	22	0.12329629455722824
1111	23	0.16001105309354102
1111	24	0.3116310086674332
1111	25	0.37431396694473307
1112	1	0.16519631600035822
1112	2	-0.08417940160650872
1112	3	0.14067017035731766
1112	4	-0.1686615269206868
1112	5	0.20161791312411736
1112	6	0.19241014813264457
1112	7	0.15089702466335098
1112	8	0.334249860022247
1112	9	0.10525720234466007
1112	10	0.42649831812437355
1112	11	0.26964749654757725
1112	12	0.29610549729461866
1112	13	0.2747689962133464
1112	14	0.36274174449918917
1112	15	0.23475940996923583
1112	16	0.0949111201036601
1112	17	0.23549403683583137
1112	18	0.28412538043275504
1112	19	0.373185995765283
1112	20	0.5478702143954806
1112	21	0.5732805491300539
1112	22	0.4802752140411428
1112	23	0.5588425355146995
1112	24	0.5534862432837713
1112	25	0.5850114862132472
1113	1	-0.25663957309092567
1113	2	-0.621659170194647
1113	3	-0.2871606822572339
1113	4	-0.3122008034719528
1113	5	-0.1911028614985142
1113	6	-0.30330380818463
1113	7	-0.0693952210438411
1113	8	0.07751563245699344
1113	9	0.03280405197783409
1113	10	-0.3819062093435619
1113	11	-0.08499032115787486
1113	12	-0.2115146805804713
1113	13	0.022648808782548713
1113	14	0.10361586062755634
1113	15	0.14445756884560268
1113	16	-0.15544064150906678
1113	17	0.026619741010577513
1113	18	0.07020678957401572
1113	19	-0.04558511830823164
1113	20	0.1691375638937629
1113	21	-0.07683814016484547
1113	22	-0.20128658269934618
1113	23	0.012301539184832677
1113	24	-0.24607106699144055
1113	25	-0.22434091749317275
1114	1	-0.6894926858785269
1114	2	-0.4220677529017678
1114	3	-0.5103686246702921
1114	4	-0.35072664496952655
1114	5	-0.622186272645358
1114	6	-0.8933552886176201
1114	7	-0.764022877201981
1114	8	-1.1369532347264553
1114	9	-0.8943096348017718
1114	10	-0.9036179090377416
1114	11	-0.8030919678400537
1114	12	-0.9012704696589182
1114	13	-0.7933004913898785
1114	14	-1.899174803726936
1114	15	-1.2895632407113808
1114	16	-1.2721878389874135
1114	17	-1.4574907393581853
1114	18	-1.5766505325541758
1114	19	-1.5200551218160214
1114	20	-0.7373165113111497
1114	21	-0.9187213260230465
1114	22	-0.8668621309758393
1114	23	-0.8344122602390982
1114	24	-1.563997818536592
1114	25	-1.4289682278134705
1115	1	-1.6920545320839437
1115	2	-1.4964892899333044
1115	3	-1.160710712089248
1115	4	-2.344746972123957
1115	5	-1.5673498117644655
1115	6	-1.5476492857072124
1115	7	-1.3085590020905506
1115	8	-1.2726034674830373
1115	9	-1.686749045710478
1115	10	-1.520526474937192
1115	11	-1.4835529858739278
1115	12	-1.9939933026608259
1115	13	-1.646148426294225
1115	14	-2.5367628969514726
1115	15	-2.378449589975773
1115	16	-1.7633914686187424
1115	17	-1.728984343313904
1115	18	-1.6035523938449643
1115	19	-1.5796789018439057
1115	20	-1.6782332903561965
1115	21	-1.6851441887385619
1115	22	-1.8747011898925834
1115	23	-2.2192209884205525
1115	24	-1.7742490127950106
1115	25	-1.5767591354498691
1116	1	-1.3572791238761006
1116	2	-1.4508971989587494
1116	3	-1.2333877835648615
1116	4	-1.4184146118478722
1116	5	-1.457670991204587
1116	6	-1.9832098165701595
1116	7	-1.445947656071695
1116	8	-1.7429447480384717
1116	9	-1.8025880846060574
1116	10	-1.8928037665222845
1116	11	-1.7194163184463562
1116	12	-2.3219651207863024
1116	13	-2.0883214379895563
1116	14	-2.5181374927497444
1116	15	-2.2123314333120447
1116	16	-2.497747847434816
1116	17	-2.4266447226500176
1116	18	-3.072907893331724
1116	19	-2.621323459299383
1116	20	-2.356075627870503
1116	21	-2.4001078288494853
1116	22	-3.628522241086685
1116	23	-3.4811909531516356
1116	24	-2.774897171721314
1116	25	-3.1039545990176656
1201	1	0.7309941655784762
1201	2	0.7854127019667452
1201	3	0.7202461585564315
1201	4	0.7288042718748216
1201	5	0.7817227536517279
1201	6	0.8545480518230306
1201	7	0.8754582883985904
1201	8	0.9203495371285584
1201	9	1.0340308058521046
1201	10	0.9757175564917517
1201	11	1.0670572858912877
1201	12	0.9928482225941053
1201	13	0.902310738176574
1201	14	0.9408927158181939
1201	15	1.0935924096084477
1201	16	1.1185015711049786
1201	17	0.9560487062708134
1201	18	0.8809365321224405
1201	19	0.7919029449927919
1201	20	0.9869372200321322
1201	21	1.0302303441972853
1201	22	1.1960554241059
1201	23	0.986785323271917
1201	24	1.0963433328493295
1201	25	1.0283878489455915
1202	1	0.6388668295694444
1202	2	0.6448406149229733
1202	3	0.7025614727418059
1202	4	0.6948811877157794
1202	5	0.7670717439941299
1202	6	0.8370083867032534
1202	7	0.7672414942182328
1202	8	0.7506949215509877
1202	9	0.9418329192347699
1202	10	0.9273825508477032
1202	11	0.9829883971417601
1202	12	0.9711264741932553
1202	13	0.9594410529890638
1202	14	0.982027639937094
1202	15	1.1918576485398447
1202	16	1.0228937384196684
1202	17	1.0894050338764814
1202	18	1.1215258648704705
1202	19	1.226169375445572
1202	20	1.3337914467974699
1202	21	1.2333808208058343
1202	22	1.1900272665742264
1202	23	1.218653816938037
1202	24	1.0249892315073055
1202	25	1.1586556276988844
1203	1	0.8139353928513309
1203	2	0.5533747556709052
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2211	14	-1.2074954089593035
2211	15	-0.6618232538031421
2211	16	-0.42268101302489924
2211	17	-0.19128911092685996
2211	18	-0.4164651169800848
2211	19	-0.3445609588784606
2211	20	-0.42351293366207443
2211	21	-0.5371735634254122
2211	22	-0.4010547227744752
2211	23	-0.4123215085602041
2211	24	-0.5072423550231804
2211	25	-0.6734185843916976
2212	1	-0.1338430315285528
2212	2	-0.03132763327518262
2212	3	-0.12114340246670352
2212	4	0.1236887313324786
2212	5	-0.03336166907146776
2212	6	-0.10533751233051447
2212	7	-0.022556054014543747
2212	8	-0.2902056989968571
2212	9	-0.33988419645045553
2212	10	-0.30198934772445796
2212	11	-0.13735231404908887
2212	12	-0.09256400199722492
2212	13	-0.06991274845660911
2212	14	-0.07868828500966174
2212	15	-0.14413681478855978
2212	16	-0.034886517877609435
2212	17	-0.24147810589627738
2212	18	-0.03177286098691923
2212	19	0.03498615121955595
2212	20	-0.27111661193163883
2212	21	-0.23743487438609634
2212	22	-0.20247619548475626
2212	23	-0.5500941504136989
2212	24	-0.31896455787362044
2212	25	-0.2843858960267127
2213	1	-0.2532188368596948
2213	2	-0.34459523091561195
2213	3	-0.2916899408191007
2213	4	-0.33731065916848024
2213	5	-0.23199984901838633
2213	6	-0.09702846812899679
2213	7	-0.5253953924481252
2213	8	-0.22388061488536692
2213	9	-0.36627631919216697
2213	10	-0.16645849786480937
2213	11	-0.3707492908586829
2213	12	-0.478648631533936
2213	13	-0.35990512502052496
2213	14	-0.18041499015056672
2213	15	-0.36877855400941684
2213	16	-0.44977069739982056
2213	17	-0.4623169594868415
2213	18	-0.5572828701137382
2213	19	-0.9144861278495711
2213	20	-0.3857955161864197
2213	21	-1.1495161726628993
2213	22	-0.6248244518351669
2213	23	-0.7478326145638121
2213	24	-0.8338392785720927
2213	25	-0.7367993681479277
2214	1	-1.2018244579587822
2214	2	-1.1631719623546317
2214	3	-0.9601333408664559
2214	4	-1.6033930228495024
2214	5	-1.4861743782433123
2214	6	-2.732177051789325
2214	7	-1.4275883099501314
2214	8	-2.10186565113624
2214	9	-1.5293395255140538
2214	10	-1.8578875043109235
2214	11	-1.4609263383559679
2214	12	-1.4932032004078692
2214	13	-1.6998387382225744
2214	14	-1.7090597531699316
2214	15	-2.101333361344288
2214	16	-1.6067463885562319
2214	17	-1.704203599003982
2214	18	-1.682932867961874
2214	19	-3.475114497134296
2214	20	-2.19895551762707
2214	21	-2.448359221025875
2214	22	-2.183397103173675
2214	23	-2.178823786438116
2214	24	-1.6139473453260749
2214	25	-1.481392682374043
2215	1	-1.653135959090502
2215	2	-1.4981968936747698
2215	3	-1.4541604687038117
2215	4	-1.364505867818174
2215	5	-2.1344747286874686
2215	6	-2.0807139958440715
2215	7	-2.1945190996990505
2215	8	-3.080704230636581
2215	9	-2.4509821694341554
2215	10	-1.9944003972069666
2215	11	-2.517128420047751
2215	12	-3.0097136376521973
2215	13	-2.6418441230030396
2215	14	-2.653264421727428
2215	15	-3.8360261473272566
2215	16	-3.0759550982680324
2215	17	-3.027830828439974
2215	18	-3.495643645282346
2215	19	-4.112651873694652
2215	20	-3.73200840517179
2215	21	-2.886345583990959
2215	22	-3.69625633939485
2215	23	-3.654075256897901
2215	24	-2.737242930040587
2215	25	-2.967495722520919
2216	1	-1.6652349792696626
2216	2	-1.4030505275989817
2216	3	-1.8765439485113262
2216	4	-1.3515847298964658
2216	5	-1.442310084676997
2216	6	-1.8500889432796228
2216	7	-3.2528895894092855
2216	8	-2.21912483644682
2216	9	-2.4117961602415647
2216	10	-2.3656661439562257
2216	11	-2.7939499382793436
2216	12	-2.3014449361146703
2216	13	-2.823381421436654
2216	14	-2.782268857874506
2216	15	-2.922799768377317
2216	16	-2.6766588996900396
2216	17	-2.8667426770616373
2216	18	-2.475557493530424
2216	19	-2.7771506210521295
2216	20	-3.170245258014859
2216	21	-3.0808760344548034
2216	22	-3.4787029524374375
2216	23	-2.552751146223393
2216	24	-2.8216805122019935
2216	25	-2.5922905830533765
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77278.0
3	1.0
4	1252.0
5	11236.0
6	12685.0
7	9941.0
8	7684.0
9	6748.0
10	5668.0
11	5157.0
12	4754.0
13	4441.0
14	4277.0
15	4382.0
16	4338.0
17	4596.0
18	5094.0
19	5844.0
20	7641.0
21	9612.0
22	12102.0
23	14859.0
24	17542.0
25	20200.0
26	22868.0
27	24888.0
28	28230.0
29	33628.0
30	42080.0
31	53925.0
32	68565.0
33	88310.0
34	115369.0
35	151458.0
36	208468.0
37	325467.0
38	1081028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.75240938357386	19.790070579236215	13.859966506667861	27.597553530522056
2	13.09850360607192	20.72466015940109	41.00696590281314	25.169870331713845
3	16.580842143638353	25.13615199135279	30.50532136027272	27.77768450473614
4	11.122751234741663	17.783398802364562	38.30094269007345	32.79290727282033
5	12.403800036081861	38.39246105099023	36.46352151771031	12.740217395217593
6	31.055909815212885	38.25524218278358	18.221708535717415	12.467139466286122
7	26.977286532150384	31.916139194317893	23.00201978356206	18.10455448996966
8	24.267636990821813	37.99395854016187	20.717439355406956	17.020965113609364
9	25.92945998021226	15.058159484079026	20.46135671603471	38.55102381967401
10	14.246155225180235	29.49003912002356	35.20345933694441	21.060346317851792
11	33.53566130425588	22.56913616323326	25.391187620150557	18.5040149123603
12	22.66444971009388	24.994572880714916	32.53644965252444	19.80452775666676
13	28.473870119735967	22.32081179962135	26.11037200020468	23.09494608043801
14	20.20618301180867	22.555684324438417	28.02055753683738	29.217575126915523
15	22.266911589808373	31.700303622077463	23.597731729206686	22.43505305890748
16	21.521037992366104	28.425647875650732	28.35380273491302	21.699511397070143
17	21.12407819917815	29.008731492510638	28.313594810285863	21.55359549802535
18	21.013433429472	28.00125519918292	30.219400022785464	20.76591134855962
19	23.01686478430921	27.201520341037376	27.853757519356048	21.927857355297366
20	23.041578186562965	27.826339674052093	28.313486606457555	20.818595532927393
21	22.28278314831271	27.201729444162627	27.56825296318815	22.947234444336516
22	22.607090076826555	28.475273314095052	27.57461297908666	21.343023629991738
23	21.63061796854513	28.18622042711591	28.119863320349726	22.06329828398923
24	22.61079342744587	27.27617958798616	28.63941893536775	21.473608049200216
25	22.253149862204772	27.715149639941504	28.24834162023523	21.78335887761849
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	161.0
1	161.0
2	342.5
3	524.0
4	524.0
5	524.0
6	1257.5
7	1991.0
8	1991.0
9	1991.0
10	3097.0
11	4203.0
12	4203.0
13	4203.0
14	7911.5
15	11620.0
16	11620.0
17	11620.0
18	21664.5
19	31709.0
20	31709.0
21	31709.0
22	50649.0
23	69589.0
24	69589.0
25	69589.0
26	99717.5
27	129846.0
28	129846.0
29	129846.0
30	167823.0
31	205800.0
32	205800.0
33	205800.0
34	231731.5
35	257663.0
36	257663.0
37	257663.0
38	275910.5
39	294158.0
40	294158.0
41	294158.0
42	305587.5
43	317017.0
44	317017.0
45	317017.0
46	319915.5
47	322814.0
48	322814.0
49	322814.0
50	308406.0
51	293998.0
52	293998.0
53	293998.0
54	258005.0
55	222012.0
56	222012.0
57	222012.0
58	189585.5
59	157159.0
60	157159.0
61	157159.0
62	126197.0
63	95235.0
64	95235.0
65	95235.0
66	72880.5
67	50526.0
68	50526.0
69	50526.0
70	36538.0
71	22550.0
72	22550.0
73	22550.0
74	15560.5
75	8571.0
76	8571.0
77	8571.0
78	5873.5
79	3176.0
80	3176.0
81	3176.0
82	2071.0
83	966.0
84	966.0
85	966.0
86	620.5
87	275.0
88	275.0
89	275.0
90	159.5
91	44.0
92	44.0
93	44.0
94	25.5
95	7.0
96	7.0
97	7.0
98	4.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08050795965487909
2	7.994832140504378E-5
3	0.0
4	0.02750222256333506
5	0.0697549104259007
6	0.08142736535103709
7	0.03409795907925117
8	0.0622797423745291
9	0.043092145237318594
10	0.1055317842546578
11	0.0026782687670689666
12	0.011552532443028826
13	0.004477105998682451
14	0.005956149944675761
15	0.006036098266080805
16	0.016109586763116322
17	0.026742713509987144
18	8.394573747529597E-4
19	0.002878139570581576
20	0.004477105998682451
21	1.1992248210756566E-4
22	2.798191249176532E-4
23	0.0
24	3.997416070252189E-5
25	0.0031179845347967076
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2501616.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.15548884965933
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.09498860828795	56.081635804637095
2	12.752167853598518	17.63764803617033
3	3.3188263703132095	6.885451801384517
4	1.2172692985311524	3.3672341360641505
5	0.5587888478003061	1.9321657966684032
6	0.2810567781628603	1.166197133301772
7	0.16471383846466975	0.7973606213529641
8	0.11016768106212409	0.6094959871427639
9	0.08467460523042715	0.52701423460759
>10	0.3746768616078836	4.481568238966024
>50	0.022830609604153507	1.105662841756507
>100	0.016538641282125816	2.3278027994019377
>500	0.0019684246642361907	1.0264003691851658
>1k	0.0013315813904964129	2.054362199360963
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4410	0.17628604869812153	No Hit
GTCCTACAGTGGACATTTCTAAATT	4377	0.1749669013949383	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3998	0.15981669448868252	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3859	0.154260286151032	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3731	0.14914359358110918	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3156	0.1261584511771591	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3005	0.12012235291107827	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2860	0.1143260996092126	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.997416070252189E-5	0.0
5	0.0	0.0	0.0	3.997416070252189E-5	0.0
6	0.0	0.0	0.0	3.997416070252189E-5	0.0
7	0.0	0.0	0.0	3.997416070252189E-5	0.0
8	0.0	0.0	0.0	3.997416070252189E-5	0.0
9	0.0	0.0	0.0	7.994832140504378E-5	0.0
10	0.0	0.0	0.0	7.994832140504378E-5	0.0
11	0.0	0.0	0.0	7.994832140504378E-5	0.0
12	0.0	0.0	0.0	7.994832140504378E-5	0.0
13	0.0	0.0	0.0	7.994832140504378E-5	3.997416070252189E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACCG	40	0.005220226	14.274594	5
TAGGACC	1020	0.0	13.155019	4
TCCAACG	270	0.0	13.003974	18
GGCGTGC	110	3.801506E-8	12.094565	8
CCAACGA	205	0.0	12.03532	19
CGTGCGC	80	2.8673472E-5	11.875262	10
GGCGAGG	1095	0.0	11.872566	19
GCACCGT	90	7.3481315E-6	11.627655	6
GCCGGTT	165	7.2759576E-12	11.504816	11
AATGTCC	665	0.0	11.003325	8
TGGCGAG	2530	0.0	10.91467	18
AGGACCT	1765	0.0	10.406032	5
GGTATCA	1405	0.0	10.355785	1
TTAGGAC	1660	0.0	10.25364	3
AAGGCGT	130	3.769528E-7	10.245346	6
TAGGACA	995	0.0	10.233712	4
TGTAGGA	2925	0.0	10.07727	2
ATGGCGA	2795	0.0	10.051793	17
GTAGGAC	2865	0.0	9.923839	3
TAGACAG	470	0.0	9.92135	5
>>END_MODULE