FastQCFastQC Report
Thu 26 May 2016
SRR1512966_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512966_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222038
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT36220.16300351299122698No Hit
GTCCTAAAGTGTGTATTTCTCATTT33080.14887234151711176No Hit
CTGTAGGACGTGGAATATGGCAAGA31700.14266182666543056No Hit
CTTTAGGACGTGAAATATGGCGAGG28240.1270905358054183No Hit
GTATCAACGCAGAGTACTTTTTTTT23690.106613838287194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG1501.8189894E-1212.66054419
TAGGACC8150.012.1203864
GACGGAT550.003081328712.0845217
CTAGGGT1658.0035534E-1110.9373074
GTCCTAC20100.010.7133221
GGCGAGG8250.010.588819519
GACGTGG9000.010.549987
TGTAGGA23550.010.3249942
GTCTTAC2400.010.3222381
AGGACGT20650.010.2566615
GGACGTG19950.010.2326866
GTCCTAA10450.010.2120631
TTAGGAC12950.010.1216213
TCCTACA21350.010.0987762
TCAATAC1606.193659E-910.0918253
GGCGAAA856.624694E-410.05396219
GTAGGAC23150.09.9700193
ATGGCAA17600.09.93350117
TGGCGAG19750.09.814321518
CCTACAG21550.09.7846773
Produced by FastQC (version 0.11.5)