##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512966_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2222038 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.380944430293273 31.0 30.0 33.0 16.0 34.0 2 28.429383745912535 31.0 28.0 34.0 16.0 34.0 3 28.565659993213437 31.0 28.0 34.0 16.0 34.0 4 31.93886423184482 35.0 32.0 37.0 19.0 37.0 5 32.03536483174455 35.0 33.0 37.0 19.0 37.0 6 32.04819719554751 35.0 33.0 37.0 19.0 37.0 7 31.825699650501026 35.0 32.0 37.0 17.0 37.0 8 31.8410963268855 35.0 32.0 37.0 17.0 37.0 9 33.096015909718915 38.0 33.0 39.0 17.0 39.0 10 32.918482042161294 37.0 33.0 39.0 17.0 39.0 11 33.0288001375314 37.0 33.0 39.0 17.0 39.0 12 32.9222637056612 37.0 33.0 39.0 16.0 39.0 13 32.94175122117623 38.0 33.0 39.0 16.0 39.0 14 33.881440821444095 39.0 33.0 41.0 13.0 41.0 15 33.81830553752906 39.0 33.0 41.0 11.0 41.0 16 33.73337449674578 39.0 33.0 41.0 10.0 41.0 17 33.66849486822458 39.0 33.0 41.0 10.0 41.0 18 33.382156380763966 38.0 33.0 41.0 10.0 41.0 19 33.6019649528946 39.0 33.0 41.0 10.0 41.0 20 33.663827531302346 39.0 33.0 41.0 8.0 41.0 21 33.58248058764072 39.0 33.0 41.0 2.0 41.0 22 33.491475393310104 39.0 33.0 41.0 2.0 41.0 23 33.22232338060825 39.0 33.0 41.0 2.0 41.0 24 33.22232158045902 39.0 33.0 41.0 2.0 41.0 25 33.130336204871384 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6334462394345692 1101 2 0.9321932865309996 1101 3 0.9728853499782417 1101 4 0.9430891599626285 1101 5 0.7681361763113657 1101 6 1.0486458607052995 1101 7 0.986071590880897 1101 8 0.8615193361272055 1101 9 0.7808639650025953 1101 10 0.7876869766066719 1101 11 1.0826673023197557 1101 12 1.1635541359743868 1101 13 1.1571253257050813 1101 14 1.0476603242728117 1101 15 0.7093880199833791 1101 16 1.3115885898703894 1101 17 1.3236518321560666 1101 18 1.5654523915338103 1101 19 0.8428133527940531 1101 20 1.11571699274063 1101 21 1.1735974332229517 1101 22 1.1223225172476177 1101 23 1.466742121937898 1101 24 1.1285360061311636 1101 25 1.414778476141791 1102 1 0.9319231751134787 1102 2 1.000256595437925 1102 3 0.908067388390176 1102 4 1.03785377089633 1102 5 0.9760661454498987 1102 6 0.9494665317209936 1102 7 1.1601286872753889 1102 8 1.0915674717031614 1102 9 1.244963881346841 1102 10 1.4485438247151663 1102 11 1.236968099730717 1102 12 1.347662491629329 1102 13 1.2838358110961607 1102 14 1.4063146061337264 1102 15 1.5576640947051317 1102 16 1.5315693785403965 1102 17 1.339920798819783 1102 18 1.7103777783746565 1102 19 1.6815090670452904 1102 20 1.6861800421002044 1102 21 1.529191878427092 1102 22 1.745721293782175 1102 23 2.0407751203800757 1102 24 1.8222129916817735 1102 25 1.6460392179967513 1103 1 0.6062439366878891 1103 2 0.7001559306687319 1103 3 0.7143643767012549 1103 4 0.9137196412493687 1103 5 0.38958065579270595 1103 6 0.7319146480125411 1103 7 0.6010412735198614 1103 8 0.5652633972187324 1103 9 0.88413011083707 1103 10 0.8711036461122816 1103 11 0.8943257629117198 1103 12 1.0721891339446188 1103 13 1.048594023161968 1103 14 1.2200022416094853 1103 15 1.2706166538838133 1103 16 1.3043637255227694 1103 17 1.1869702167226137 1103 18 1.476074451657695 1103 19 1.5789491869229835 1103 20 1.3796581036729165 1103 21 1.1494543050042907 1103 22 1.3407207946180293 1103 23 1.704081890350885 1103 24 1.4611992937041407 1103 25 1.4078243040161098 1104 1 0.7416068850659663 1104 2 0.7539610184799983 1104 3 0.7299404566185821 1104 4 1.0008101830607181 1104 5 0.7016796375531698 1104 6 0.7386862341526665 1104 7 0.877105674373567 1104 8 0.8718297781725788 1104 9 0.9918994731099247 1104 10 1.079824210960247 1104 11 1.0507238717517922 1104 12 0.9646162010806023 1104 13 1.01850734406014 1104 14 1.2231702892210095 1104 15 1.355613476988033 1104 16 1.2172229904472402 1104 17 1.1461988878229619 1104 18 1.3327554687180125 1104 19 1.3448354958012345 1104 20 1.3100186497724167 1104 21 1.3634382449293838 1104 22 1.3126079012795984 1104 23 1.5718976073902198 1104 24 1.464721268178451 1104 25 1.1509187686241091 1105 1 0.7260594348548146 1105 2 0.8601355495017486 1105 3 0.862057844856043 1105 4 1.0389675279615425 1105 5 0.6050670736885166 1105 6 0.4178126897439256 1105 7 0.7471565525334043 1105 8 0.7303026237636452 1105 9 0.6823707989223564 1105 10 0.9996179571094075 1105 11 0.9161501508536531 1105 12 0.900248890218613 1105 13 0.9058691320185019 1105 14 1.013018738476248 1105 15 1.168344268665841 1105 16 1.120572336492991 1105 17 1.1108649907350525 1105 18 1.1345094615634537 1105 19 1.2184850905641156 1105 20 1.2800966531759812 1105 21 1.143384944303591 1105 22 0.8578225907306205 1105 23 1.1670213906190412 1105 24 1.124038247529164 1105 25 1.2246004610608168 1106 1 0.6400076653867224 1106 2 0.6674274397828626 1106 3 0.6787896439517596 1106 4 0.6668727666378622 1106 5 0.733798552846622 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1.2644690630494324 1107 19 1.1280986671537079 1107 20 1.0247475301576614 1107 21 1.0364597470647965 1107 22 0.9976070199526177 1107 23 1.0021434964581033 1107 24 1.0225556927098296 1107 25 1.0436422871072608 1108 1 0.5229392337640242 1108 2 0.5946995549881215 1108 3 0.5055113298934621 1108 4 0.6639081526376422 1108 5 0.4769947384880311 1108 6 0.6107475190068854 1108 7 0.7153963976838327 1108 8 0.5994956852920588 1108 9 0.6877671971691015 1108 10 0.6967675600339476 1108 11 0.5242517111131733 1108 12 0.5750692381341693 1108 13 0.6846465313061927 1108 14 0.737398484925329 1108 15 0.8011218002166345 1108 16 0.6515339846438835 1108 17 0.7719868870827469 1108 18 0.9506914072681738 1108 19 0.8838508520975523 1108 20 0.8309961694618266 1108 21 0.6663453105197803 1108 22 0.7060026694907577 1108 23 0.7635262427037404 1108 24 0.8127150632694864 1108 25 0.9190774590444377 1109 1 0.12063794955767193 1109 2 0.12158058023356588 1109 3 0.35660309488828545 1109 4 0.45503863595508065 1109 5 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6 -1.5817665470360254 2214 7 -1.7524460692607562 2214 8 -1.4082158353643628 2214 9 -1.6258644008691867 2214 10 -1.7390551771840492 2214 11 -1.3671753317963358 2214 12 -1.888471181139085 2214 13 -1.8264093701379274 2214 14 -2.8305817853480697 2214 15 -3.224058942430684 2214 16 -2.31032761907548 2214 17 -2.9226069823254086 2214 18 -2.27135182521366 2214 19 -4.445316911861244 2214 20 -2.5546052045963634 2214 21 -2.5912212019462864 2214 22 -2.798351220057885 2214 23 -2.9501520627128244 2214 24 -2.9961533759587446 2214 25 -2.750574007848808 2215 1 -1.1614285783410132 2215 2 -2.9325117422961675 2215 3 -1.608954102090216 2215 4 -1.1360285451092764 2215 5 -1.0346522257136783 2215 6 -1.573510814886486 2215 7 -2.0536414297481542 2215 8 -2.087149490404215 2215 9 -1.9464444187943357 2215 10 -3.5867669169311895 2215 11 -2.427425039833654 2215 12 -2.8154937400200524 2215 13 -1.99642609502191 2215 14 -3.491235378745028 2215 15 -3.2447072967073574 2215 16 -2.963944933754739 2215 17 -3.20025442457462 2215 18 -2.035469896411378 2215 19 -2.7734010496798938 2215 20 -2.9715138082306325 2215 21 -2.536394246234373 2215 22 -2.2962302524501936 2215 23 -2.986565350453546 2215 24 -2.736777759945962 2215 25 -2.5988325260188283 2216 1 -2.4626731053919926 2216 2 -2.4531053596639794 2216 3 -3.086952128475339 2216 4 -4.000136538249528 2216 5 -2.958141116944507 2216 6 -2.916977287385219 2216 7 -2.6685565930504147 2216 8 -3.349582785095574 2216 9 -3.665328847356186 2216 10 -3.011247405927808 2216 11 -4.001421226733065 2216 12 -4.139463580118925 2216 13 -4.646176518965881 2216 14 -4.077092416214967 2216 15 -4.042325545938507 2216 16 -3.1438600491537265 2216 17 -3.3466372483767977 2216 18 -3.4648844226000968 2216 19 -3.764031169342271 2216 20 -3.664358017203874 2216 21 -3.9714326167566156 2216 22 -4.759991425888785 2216 23 -4.433043022737671 2216 24 -4.309490139109954 2216 25 -4.416637214791258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 139484.0 3 3.0 4 1139.0 5 6383.0 6 7725.0 7 6923.0 8 6073.0 9 5594.0 10 5268.0 11 5037.0 12 4672.0 13 4641.0 14 4653.0 15 4770.0 16 4935.0 17 5214.0 18 6127.0 19 7142.0 20 9207.0 21 11914.0 22 14885.0 23 17782.0 24 20668.0 25 23323.0 26 25966.0 27 28622.0 28 32430.0 29 37929.0 30 46501.0 31 57543.0 32 71598.0 33 89695.0 34 115006.0 35 149114.0 36 200857.0 37 305329.0 38 737886.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.266474350549196 18.710081872105714 12.365998618828824 25.657445158516268 2 14.804584846630767 20.265331858417042 41.40053116796172 23.52955212699047 3 18.41750787180727 25.087857202669888 29.770333213579214 26.724301711943628 4 12.272813015055084 17.02758320453908 38.775901601040495 31.92370217936534 5 12.981184607495273 38.20288711890062 36.08443897569995 12.731489297904153 6 31.446713851283832 37.61430806597684 18.422706231646345 12.516271851092982 7 27.61647508095393 31.811495502859604 22.8246416826534 17.747387733533067 8 24.841751496507325 37.071383106775315 20.94462045117141 17.14224494554595 9 26.654759209336433 15.098913969671093 20.181789782663394 38.06453703832908 10 14.709254355344234 28.95311942260939 35.060841805903095 21.276784416143276 11 34.13632347308015 22.343675518643437 25.19340677246667 18.326594235809747 12 23.20420203737055 24.73022687814893 32.007675587158566 20.05789549732196 13 28.902864890218073 22.12635477537219 25.812730184651866 23.158050149757877 14 20.937642362355298 22.03323603926095 27.791202479108186 29.237919119275567 15 22.896940046078925 30.968449711579062 23.274223622336486 22.860386620005528 16 22.239962548755262 28.027350211901126 27.657474156615525 22.07521308272809 17 21.808532427048988 28.546516469542322 27.846591868198644 21.798359235210047 18 21.795323386490864 27.515193228315525 29.476169198420948 21.213314186772656 19 23.53969107109578 27.05621742602461 27.375508781975277 22.028582720904332 20 23.62186754317615 27.388850702148375 27.763815985224653 21.225465769450818 21 23.009424003637253 26.932978610865398 27.033993486251127 23.023603899246215 22 23.272936623290807 28.170692238753432 26.940176603323117 21.616194534632644 23 22.338723617258033 27.827153202660508 27.531545854925078 22.302577325156378 24 23.01900279120516 27.035889559920435 28.079857005312785 21.865250643561616 25 22.84098663800166 27.2859982606535 27.663756952434454 22.209258148910386 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 223.0 1 223.0 2 383.0 3 543.0 4 543.0 5 543.0 6 943.5 7 1344.0 8 1344.0 9 1344.0 10 2204.5 11 3065.0 12 3065.0 13 3065.0 14 5971.5 15 8878.0 16 8878.0 17 8878.0 18 16817.5 19 24757.0 20 24757.0 21 24757.0 22 40004.5 23 55252.0 24 55252.0 25 55252.0 26 80438.0 27 105624.0 28 105624.0 29 105624.0 30 136549.5 31 167475.0 32 167475.0 33 167475.0 34 192583.5 35 217692.0 36 217692.0 37 217692.0 38 236502.5 39 255313.0 40 255313.0 41 255313.0 42 267097.0 43 278881.0 44 278881.0 45 278881.0 46 284371.5 47 289862.0 48 289862.0 49 289862.0 50 278915.0 51 267968.0 52 267968.0 53 267968.0 54 239281.0 55 210594.0 56 210594.0 57 210594.0 58 180169.5 59 149745.0 60 149745.0 61 149745.0 62 122077.0 63 94409.0 64 94409.0 65 94409.0 66 73094.5 67 51780.0 68 51780.0 69 51780.0 70 37760.0 71 23740.0 72 23740.0 73 23740.0 74 16627.0 75 9514.0 76 9514.0 77 9514.0 78 6558.5 79 3603.0 80 3603.0 81 3603.0 82 2418.0 83 1233.0 84 1233.0 85 1233.0 86 806.0 87 379.0 88 379.0 89 379.0 90 249.0 91 119.0 92 119.0 93 119.0 94 74.0 95 29.0 96 29.0 97 29.0 98 22.5 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35890475320404064 2 0.040278339074309256 3 0.03690305926361295 4 0.03577796599338085 5 0.041898473383443484 6 0.018451529631806476 7 0.018181507246950772 8 0.023941984790539134 9 0.020746719903079965 10 0.02056670497984283 11 0.019126585593945738 12 0.020206675133368556 13 0.02034168632579641 14 0.026732216100714752 15 0.028442357871467542 16 0.021736801980884214 17 0.02308691390516274 18 0.08865734969428965 19 0.025022074329961955 20 0.022456861673832762 21 0.026237175061812626 22 0.020206675133368556 23 0.022951902712734888 24 0.018766555747471467 25 0.02313191763597202 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2222038.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.01636104998794 #Duplication Level Percentage of deduplicated Percentage of total 1 83.43015010722767 60.91765962684025 2 11.293451454160023 16.492134577549187 3 2.9428839226637336 6.446360250762598 4 0.9994852475537731 2.9191510279809143 5 0.468401018605007 1.7100468945322653 6 0.23854796798758354 1.045074273499342 7 0.13705055349713782 0.7004852887373391 8 0.08296284055034782 0.48461157754854206 9 0.07564384610501827 0.49709145405723637 >10 0.2974702228971579 3.7339317373943173 >50 0.01801119201954416 0.89583694684114 >100 0.01310244162670156 1.8737695929615124 >500 0.001851642460688079 0.8736636565038763 >1k 9.875426456987383E-4 1.410183094791516 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3622 0.16300351299122698 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3308 0.14887234151711176 No Hit CTGTAGGACGTGGAATATGGCAAGA 3170 0.14266182666543056 No Hit CTTTAGGACGTGAAATATGGCGAGG 2824 0.1270905358054183 No Hit GTATCAACGCAGAGTACTTTTTTTT 2369 0.106613838287194 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 4.500373080928409E-5 3 0.0 0.0 0.0 0.0 4.500373080928409E-5 4 0.0 0.0 0.0 4.500373080928409E-5 4.500373080928409E-5 5 0.0 0.0 0.0 4.500373080928409E-5 4.500373080928409E-5 6 0.0 0.0 0.0 4.500373080928409E-5 4.500373080928409E-5 7 0.0 0.0 0.0 4.500373080928409E-5 4.500373080928409E-5 8 4.500373080928409E-5 0.0 0.0 4.500373080928409E-5 4.500373080928409E-5 9 4.500373080928409E-5 0.0 0.0 4.500373080928409E-5 4.500373080928409E-5 10 4.500373080928409E-5 0.0 0.0 9.000746161856818E-5 4.500373080928409E-5 11 4.500373080928409E-5 0.0 0.0 9.000746161856818E-5 4.500373080928409E-5 12 4.500373080928409E-5 0.0 0.0 9.000746161856818E-5 4.500373080928409E-5 13 4.500373080928409E-5 0.0 0.0 9.000746161856818E-5 4.500373080928409E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCCCG 150 1.8189894E-12 12.660544 19 TAGGACC 815 0.0 12.120386 4 GACGGAT 55 0.0030813287 12.084521 7 CTAGGGT 165 8.0035534E-11 10.937307 4 GTCCTAC 2010 0.0 10.713322 1 GGCGAGG 825 0.0 10.5888195 19 GACGTGG 900 0.0 10.54998 7 TGTAGGA 2355 0.0 10.324994 2 GTCTTAC 240 0.0 10.322238 1 AGGACGT 2065 0.0 10.256661 5 GGACGTG 1995 0.0 10.232686 6 GTCCTAA 1045 0.0 10.212063 1 TTAGGAC 1295 0.0 10.121621 3 TCCTACA 2135 0.0 10.098776 2 TCAATAC 160 6.193659E-9 10.091825 3 GGCGAAA 85 6.624694E-4 10.053962 19 GTAGGAC 2315 0.0 9.970019 3 ATGGCAA 1760 0.0 9.933501 17 TGGCGAG 1975 0.0 9.8143215 18 CCTACAG 2155 0.0 9.784677 3 >>END_MODULE