FastQCFastQC Report
Thu 26 May 2016
SRR1512966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222038
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT32800.1476122370544518No Hit
CTGTAGGACGTGGAATATGGCAAGA31110.14000660654768282No Hit
GTCCTAAAGTGTGTATTTCTCATTT30370.1366763304677958No Hit
CTTTAGGACGTGAAATATGGCGAGG29740.13384109542681089No Hit
GTATCAACGCAGAGTACTTTTTTTT29380.13222096111767664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC551.9504577E-413.8267563
TAGGACC6950.011.908864
AACCGGC657.999253E-411.6969257
TCCAACG1105.0360177E-711.21603618
GGCGAGG8500.011.16528519
TGTAGGA21100.010.9908642
GTAGGAC20400.010.717433
CCAACGA803.807108E-410.67680419
AGGACGT19300.010.5978285
GGACGTG19000.010.3620276
TGGCGAG19200.010.33079618
GACGTGG9200.010.3301667
ATAAGAC2400.010.2980523
TAAAGGT2800.010.1929284
GGACCTG12250.010.1709896
GGCGTGC1502.4343535E-810.1364228
GTACCGT856.5252656E-410.0705126
GCACCTA951.6253816E-410.01162056
TATGTCG1054.1374395E-59.94555416
CTGTAGG22000.09.8952181
Produced by FastQC (version 0.11.5)