##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512966_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2222038
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.46790333918682	31.0	31.0	34.0	27.0	34.0
2	30.589380559648394	31.0	31.0	34.0	27.0	34.0
3	30.702201312488807	31.0	31.0	34.0	27.0	34.0
4	34.19954429222182	37.0	35.0	37.0	30.0	37.0
5	33.86627996460906	37.0	35.0	37.0	30.0	37.0
6	33.741270851353576	37.0	35.0	37.0	29.0	37.0
7	33.60697791846944	37.0	35.0	37.0	28.0	37.0
8	33.48525362752572	37.0	35.0	37.0	28.0	37.0
9	34.93929851784713	39.0	35.0	39.0	28.0	39.0
10	34.8178352485421	39.0	35.0	39.0	27.0	39.0
11	34.86500185865408	39.0	35.0	39.0	27.0	39.0
12	34.72024285813294	39.0	35.0	39.0	27.0	39.0
13	34.71599495598185	39.0	35.0	39.0	27.0	39.0
14	35.770704641414774	40.0	36.0	41.0	27.0	41.0
15	35.78797257292629	40.0	36.0	41.0	27.0	41.0
16	35.76413454675392	40.0	36.0	41.0	27.0	41.0
17	35.73289655712458	40.0	36.0	41.0	27.0	41.0
18	35.65035611452189	40.0	36.0	41.0	27.0	41.0
19	35.62347583614682	40.0	36.0	41.0	27.0	41.0
20	35.602255676995625	40.0	36.0	41.0	27.0	41.0
21	35.54414055925236	40.0	36.0	41.0	27.0	41.0
22	35.50803181583753	40.0	36.0	41.0	27.0	41.0
23	35.402554771790584	40.0	36.0	41.0	26.0	41.0
24	35.389100906465146	40.0	36.0	41.0	26.0	41.0
25	35.295136716833824	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6325627455095741
1101	2	0.6111831954258982
1101	3	0.6653905866981091
1101	4	0.46356626507287046
1101	5	0.7669037557420779
1101	6	0.8089189710682732
1101	7	0.7932942946615924
1101	8	0.7613369677511201
1101	9	0.5895403448006107
1101	10	0.8515787061196605
1101	11	0.9412589984890829
1101	12	1.0137273072058974
1101	13	1.0005030640611352
1101	14	1.0978810731486917
1101	15	1.0439143197159453
1101	16	1.2542771871004064
1101	17	1.135186277970405
1101	18	0.6594706682006262
1101	19	1.1056965985917557
1101	20	0.9526283372069244
1101	21	1.1631267371363094
1101	22	1.1280312322557222
1101	23	0.9659592800373389
1101	24	0.9380504019823803
1101	25	0.9659257132195549
1102	1	0.7535203894202667
1102	2	0.6425252804383774
1102	3	0.6509316477319551
1102	4	0.5645983545630813
1102	5	0.771790713219751
1102	6	0.719231185344988
1102	7	0.70054265037016
1102	8	0.8046573103107164
1102	9	0.8811248974434065
1102	10	1.0198248108984984
1102	11	0.9638489016146394
1102	12	0.9970072161498251
1102	13	1.050933308030281
1102	14	1.1467022174092847
1102	15	1.2137789086632012
1102	16	1.0816644596278024
1102	17	1.0599354782803587
1102	18	1.00619780430479
1102	19	1.165887518724638
1102	20	1.0643629004407558
1102	21	1.0780529216562371
1102	22	1.0788995629211868
1102	23	1.0318482431081435
1102	24	1.0078134711833897
1102	25	1.0527892792606792
1103	1	0.5837491175239258
1103	2	0.5465073053091523
1103	3	0.5836682189114555
1103	4	0.49735588737415526
1103	5	0.669212644600016
1103	6	0.6695358875364832
1103	7	0.613781886368443
1103	8	0.7401527421431666
1103	9	0.8673988622161204
1103	10	0.8632167095623018
1103	11	0.8200885676118403
1103	12	0.8870800158147034
1103	13	0.9870963311249383
1103	14	1.0510526613064997
1103	15	0.8184566476388468
1103	16	0.9553356389993439
1103	17	1.155031223183336
1103	18	1.16997706739496
1103	19	1.1292121672543587
1103	20	1.1875501679053002
1103	21	0.8770794863237725
1103	22	1.081062607548418
1103	23	1.14812902000331
1103	24	1.179763284633026
1103	25	1.0737915290880906
1104	1	0.5062619789445861
1104	2	0.5926732035389293
1104	3	0.5394417594518082
1104	4	0.3789967920530444
1104	5	0.5983661392215751
1104	6	0.6278328461705556
1104	7	0.653833939357412
1104	8	0.5806985552808825
1104	9	0.7474543354255871
1104	10	0.8488490485676081
1104	11	0.8642065163128763
1104	12	0.7290718615006568
1104	13	0.8213759495950015
1104	14	1.0050310687112258
1104	15	1.0213441760364503
1104	16	1.0023063186523373
1104	17	1.0121865022397856
1104	18	0.9698837915790364
1104	19	0.961554599216143
1104	20	1.0824557687737766
1104	21	0.9839407044418991
1104	22	0.963174476268577
1104	23	0.8859740252949138
1104	24	0.9894646129929328
1104	25	1.1007568145739484
1105	1	0.4796150128760033
1105	2	0.5395282849321923
1105	3	0.47873077844553436
1105	4	0.401284594395122
1105	5	0.43735008687846033
1105	6	0.4217084060072196
1105	7	0.5621012897119684
1105	8	0.6381024718345785
1105	9	0.725052820619382
1105	10	0.6988853377450184
1105	11	0.7701052687110419
1105	12	0.7028842077258659
1105	13	0.7897915357409175
1105	14	0.8680486676076029
1105	15	0.745574636697043
1105	16	0.9434247775559967
1105	17	0.6363952563304878
1105	18	0.8439224949350077
1105	19	0.831921354185944
1105	20	1.0538927437002954
1105	21	1.001142922018225
1105	22	0.9278849644942824
1105	23	0.9419694053973515
1105	24	0.919689625017611
1105	25	1.0252336495839316
1106	1	0.43842304490029704
1106	2	0.44629461566436035
1106	3	0.44347419388452636
1106	4	0.39796198183416465
1106	5	0.5117220661950981
1106	6	0.4644925883678397
1106	7	0.6614427938576384
1106	8	0.6951928109589076
1106	9	0.5561353801075839
1106	10	0.6129385179917008
1106	11	0.49982151012464016
1106	12	0.6099367649528773
1106	13	0.5927944810115449
1106	14	0.6768136702488405
1106	15	0.8730464012483949
1106	16	0.9279660025990353
1106	17	0.7966285054814293
1106	18	0.8655279043613362
1106	19	0.8648845489570292
1106	20	0.9195811995179
1106	21	0.8312539810785182
1106	22	0.9370066053480883
1106	23	1.0340169043661405
1106	24	0.933161034012052
1106	25	0.9949543052461465
1107	1	0.45778017778603086
1107	2	0.47822359436625916
1107	3	0.4562567125736443
1107	4	0.4760019277259673
1107	5	0.608794801351749
1107	6	0.6628596189429601
1107	7	0.6726640182004004
1107	8	0.7243990164094072
1107	9	0.5911385621939971
1107	10	0.7893321364094206
1107	11	0.6413619640621775
1107	12	0.6169996308957124
1107	13	0.6909087735917225
1107	14	0.8236190939409269
1107	15	0.8805225101343268
1107	16	0.7509864357841352
1107	17	0.8252610324932803
1107	18	0.9493914503839846
1107	19	1.113628788153619
1107	20	1.1240301938790438
1107	21	0.910304462234528
1107	22	0.9139231092062943
1107	23	0.9814514759697985
1107	24	0.996927313989822
1107	25	0.9496989940325378
1108	1	0.43564961239540523
1108	2	0.43534279355885985
1108	3	0.4170503884219876
1108	4	0.3958347822023711
1108	5	0.48719769816228364
1108	6	0.4956075541183651
1108	7	0.4701890831627793
1108	8	0.43181542783229787
1108	9	0.43837301878512136
1108	10	0.6074924864386375
1108	11	0.4472295699580613
1108	12	0.5329334312206271
1108	13	0.5685752938316995
1108	14	0.6674384450685693
1108	15	0.6488429343119151
1108	16	0.409537762041154
1108	17	0.5337187089316515
1108	18	0.5428237891422043
1108	19	0.5466830930133639
1108	20	0.561506615556425
1108	21	0.6599142868017793
1108	22	0.6777781366022779
1108	23	0.5552478262267613
1108	24	0.6268006109309496
1108	25	0.5720908747421589
1109	1	0.27378254329341445
1109	2	0.3141184606701337
1109	3	0.24050021571738966
1109	4	0.37244513299157944
1109	5	0.3220717628674521
1109	6	0.09707434138447013
1109	7	0.319652961953679
1109	8	0.4567936166480564
1109	9	0.5788337928437812
1109	10	0.46141911042587935
1109	11	0.4609163954633573
1109	12	0.5235533924166305
1109	13	0.46335463477299044
1109	14	0.6210578482726845
1109	15	0.5917467355265558
1109	16	0.542012525084985
1109	17	0.5275308216234862
1109	18	0.507060903805332
1109	19	0.7774414558711911
1109	20	0.7540419787088766
1109	21	0.7633997291154557
1109	22	0.8245962421936355
1109	23	0.6187188086653208
1109	24	0.24190028078442793
1109	25	0.4276386420685796
1110	1	0.3868815792699536
1110	2	0.35978446273669107
1110	3	0.3336955694024404
1110	4	0.48671915773420693
1110	5	0.5043705089637314
1110	6	0.5567865219382853
1110	7	0.48689869805750874
1110	8	0.6262951417185647
1110	9	0.7285389469150729
1110	10	0.7294255686710329
1110	11	0.6935741102561863
1110	12	0.8220010048995547
1110	13	0.36966667411383725
1110	14	0.7183703992445771
1110	15	0.6887115730636992
1110	16	0.74466601808696
1110	17	0.6634033792918501
1110	18	0.6014936762702163
1110	19	0.7451209563316894
1110	20	0.7483775420516139
1110	21	0.7528436213441694
1110	22	0.6517631751584787
1110	23	0.777824105813167
1110	24	0.6682655937370043
1110	25	0.8221914370113765
1111	1	-0.1902558505238261
1111	2	0.015881260912443906
1111	3	-0.016440771623731365
1111	4	0.07461682433098815
1111	5	0.40793694228690924
1111	6	0.11964846285390252
1111	7	0.38350435515864234
1111	8	0.41204272155862043
1111	9	-0.02731970113470794
1111	10	0.1704457024917332
1111	11	0.312612570031078
1111	12	0.20323347212031706
1111	13	0.3836009546718273
1111	14	0.38841807986599974
1111	15	0.5868219145850873
1111	16	0.37978372230836754
1111	17	0.6050862446574996
1111	18	0.5729861796483036
1111	19	0.6674895017944564
1111	20	0.48610645441294054
1111	21	0.4568348890674798
1111	22	0.28815835510871324
1111	23	0.3090208710697482
1111	24	0.36835818483630334
1111	25	0.43090391793106875
1112	1	0.016267093708862035
1112	2	-0.12887977728397715
1112	3	0.05111501671510155
1112	4	-0.11959929710032213
1112	5	0.11799400817265138
1112	6	0.0482154311618217
1112	7	0.0021040363135185203
1112	8	0.17479907520399252
1112	9	-0.03355068926148874
1112	10	0.26207002851406
1112	11	-0.002658289028417471
1112	12	0.17843631853305197
1112	13	-0.01814294464345778
1112	14	0.09261595244774412
1112	15	-0.08826318087092488
1112	16	-0.17688201407477067
1112	17	-0.1277124305168016
1112	18	-0.12591035115873694
1112	19	-0.11715113477435324
1112	20	0.2893084775884702
1112	21	0.35315001050771144
1112	22	0.28253205127811043
1112	23	0.2975417457401619
1112	24	0.18896192610533546
1112	25	0.2847614724068137
1113	1	-0.24870106213359477
1113	2	-0.7027736425525859
1113	3	-0.21989301558230423
1113	4	-0.26773070156084344
1113	5	-0.20075759805320104
1113	6	-0.35350714384600224
1113	7	-0.08525923006064318
1113	8	0.09882190580616168
1113	9	-0.03491463784357052
1113	10	-0.4381465491384944
1113	11	-0.12859060091893326
1113	12	-0.20392673757153545
1113	13	-0.0576661467218571
1113	14	-0.14980593240863982
1113	15	-0.1619250487188424
1113	16	-0.4608248955154437
1113	17	-0.12829183998641014
1113	18	-0.06616482405966195
1113	19	-0.14844835781428856
1113	20	-0.020005296002608475
1113	21	-0.1817215615142871
1113	22	-0.41145166918485643
1113	23	-0.2792734646929418
1113	24	-0.4905984703228796
1113	25	-0.5500703820305759
1114	1	-0.7540061730024021
1114	2	-0.5703706813440306
1114	3	-0.6167974181616067
1114	4	-0.27239178888311955
1114	5	-0.5996387605851012
1114	6	-1.1383975828202182
1114	7	-0.7237498866244749
1114	8	-1.1335969413700298
1114	9	-0.9136808758298045
1114	10	-1.1038521147631855
1114	11	-0.9678661687018675
1114	12	-0.9394861924100582
1114	13	-0.9537597230944499
1114	14	-2.1298890208333106
1114	15	-1.3880199970613631
1114	16	-1.4019608063871445
1114	17	-1.531192874419645
1114	18	-1.470763250065147
1114	19	-1.5495914253946381
1114	20	-0.7622808244717874
1114	21	-0.9060079967432699
1114	22	-0.9932702594576668
1114	23	-0.7709887721064774
1114	24	-1.4738354047162403
1114	25	-1.4071635869282701
1115	1	-1.6851880578556724
1115	2	-1.3194512037925321
1115	3	-1.0655781750775475
1115	4	-2.2337645223335407
1115	5	-1.5798732032303704
1115	6	-1.5410065489018692
1115	7	-1.1530421647486548
1115	8	-1.1694203039189617
1115	9	-1.6867579603434635
1115	10	-1.497406128612333
1115	11	-1.376599819605545
1115	12	-1.9808122519809785
1115	13	-1.4545904274726098
1115	14	-2.2616688782401226
1115	15	-2.229923254934988
1115	16	-1.7317441573393282
1115	17	-1.7116619616082716
1115	18	-1.6537208197647075
1115	19	-1.408192363724865
1115	20	-1.5793423819596981
1115	21	-1.5856733878508606
1115	22	-2.055103320553272
1115	23	-2.3299523123221846
1115	24	-1.7694216468136617
1115	25	-1.5910613995749898
1116	1	-1.5101530451604397
1116	2	-1.5858274728544863
1116	3	-1.3905147516292402
1116	4	-1.4962708146037613
1116	5	-1.5004435899486879
1116	6	-2.1776836187886346
1116	7	-1.5313035637067856
1116	8	-1.8597997060702731
1116	9	-1.8454839357309964
1116	10	-2.0177443799752837
1116	11	-1.8622109591508362
1116	12	-2.660657853506194
1116	13	-2.2043033945303634
1116	14	-2.656278319992545
1116	15	-2.344117087956718
1116	16	-2.7952604808719173
1116	17	-2.660562560342491
1116	18	-3.670707076655898
1116	19	-3.0131836955461253
1116	20	-2.741747312674889
1116	21	-2.7496674231740172
1116	22	-4.377413668234475
1116	23	-4.259945370399773
1116	24	-3.376995352688045
1116	25	-3.845603825684627
1201	1	0.8960872451389221
1201	2	0.8027940052873568
1201	3	0.7885651997438359
1201	4	0.7466842835417111
1201	5	0.7634296797178308
1201	6	0.8979790613332597
1201	7	0.9312911992666031
1201	8	0.9098071835010728
1201	9	1.109880462387352
1201	10	1.0058529156091183
1201	11	1.1798415474523765
1201	12	0.9490256417439866
1201	13	1.0272793070498096
1201	14	1.0797593960042988
1201	15	1.1177309820573527
1201	16	1.1783392104923536
1201	17	1.1737312748960207
1201	18	1.1292856125106852
1201	19	0.9998175349461818
1201	20	1.315346937305229
1201	21	1.2373310499485441
1201	22	1.3906300861155927
1201	23	1.2425000539817574
1201	24	1.3537960499694464
1201	25	1.1708934651082217
1202	1	0.8386995817456828
1202	2	0.8805717070648278
1202	3	0.8869594115457886
1202	4	0.8896842328254095
1202	5	0.879833683769796
1202	6	1.046573986233092
1202	7	0.8887757574875863
1202	8	0.8803898937801975
1202	9	1.1769139825257255
1202	10	1.1086695388110286
1202	11	1.1332136642973936
1202	12	1.1580643042483558
1202	13	1.0660222234506662
1202	14	1.055197357956395
1202	15	1.2915471735033819
1202	16	1.0706901079039568
1202	17	1.2491464979276259
1202	18	1.2309121543529642
1202	19	1.4281448079039833
1202	20	1.4452984498521744
1202	21	1.4183455361256776
1202	22	1.3954761621419962
1202	23	1.3520812140771028
1202	24	1.2346734686698753
1202	25	1.3354033821470281
1203	1	0.504638228203806
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2211	12	0.10007316827149282
2211	13	0.07662994164446246
2211	14	-1.0058834706746254
2211	15	-0.3578339283097307
2211	16	-0.03894987712541109
2211	17	0.05419260940127657
2211	18	-0.07654445319889192
2211	19	0.054729439971843874
2211	20	-0.22855827989588562
2211	21	-0.1660926812172221
2211	22	0.036609579925062974
2211	23	-0.09876160385694988
2211	24	-0.15697560430236734
2211	25	-0.2805684135613902
2212	1	-0.16222995251072803
2212	2	-0.11934510412232058
2212	3	-0.036070712126669235
2212	4	0.0961593403309493
2212	5	-0.09703911524224651
2212	6	-0.1344210120099092
2212	7	-0.15137307543578515
2212	8	-0.37954299267726554
2212	9	-0.4355780699010836
2212	10	-0.38544009282606595
2212	11	-0.2757666352012933
2212	12	-0.09423504085896894
2212	13	-0.15321671820435512
2212	14	-0.28070342599348663
2212	15	-0.13788414366388224
2212	16	-0.10499478962132969
2212	17	-0.3144101500988441
2212	18	-0.19677575282190674
2212	19	-0.13387099054430251
2212	20	-0.28361346259622167
2212	21	-0.06629779322175011
2212	22	-0.1283785657002312
2212	23	-0.5348811476066828
2212	24	-0.09753509081905065
2212	25	-0.29804340292531606
2213	1	-0.1667307077508653
2213	2	-0.24473998960964138
2213	3	-0.2243580200290296
2213	4	-0.1857879219532066
2213	5	-0.06151646680000056
2213	6	0.11355127210424598
2213	7	-0.46764043296919766
2213	8	-0.14707587770529784
2213	9	-0.45380109343431485
2213	10	-0.13517327753204
2213	11	-0.13286375367263759
2213	12	-0.2857685919400126
2213	13	-0.17581140329420464
2213	14	-0.04514747490094351
2213	15	-0.2401193774546826
2213	16	-0.47266041081026344
2213	17	-0.2723489486735957
2213	18	-0.4826760213321819
2213	19	-0.7725337911854666
2213	20	-0.25883221976304327
2213	21	-1.051967246511154
2213	22	-0.44590709276425855
2213	23	-0.4183625050478099
2213	24	-0.5326076637372736
2213	25	-0.44425190919660906
2214	1	-0.9644373428301058
2214	2	-0.9452166680806329
2214	3	-0.6854954949520078
2214	4	-1.171453490104959
2214	5	-1.1921594625591325
2214	6	-2.462996458409812
2214	7	-1.1674605419359736
2214	8	-1.7869042931159633
2214	9	-1.2319797212982593
2214	10	-1.4162093548589638
2214	11	-1.2795720180928
2214	12	-1.1853775173532242
2214	13	-1.3355372742625278
2214	14	-1.4941396018011233
2214	15	-1.8397028264966195
2214	16	-1.2662292244551168
2214	17	-1.4606331765060787
2214	18	-1.4702473203567834
2214	19	-2.9819758586544367
2214	20	-1.78633465885936
2214	21	-2.194306354215584
2214	22	-1.8445335804901362
2214	23	-1.8092860357943437
2214	24	-1.116766275856655
2214	25	-0.9658240880161628
2215	1	-1.7045768015536353
2215	2	-1.5011316611637682
2215	3	-1.4506186361813427
2215	4	-1.4274153549163842
2215	5	-2.195131217743274
2215	6	-1.915166619429531
2215	7	-2.1812452981986077
2215	8	-2.704823135813566
2215	9	-2.23356197066812
2215	10	-1.8181989064060389
2215	11	-2.5003260308822064
2215	12	-3.0955994089910455
2215	13	-2.5874441651074704
2215	14	-2.6143823185040134
2215	15	-3.7833618701556624
2215	16	-3.2590164101626513
2215	17	-2.91052002727789
2215	18	-3.600887500554485
2215	19	-4.2862542399239665
2215	20	-3.6994970286589
2215	21	-2.7919933439379676
2215	22	-3.8051584375751695
2215	23	-3.7486268353121375
2215	24	-2.799351457556881
2215	25	-3.107965013342927
2216	1	-1.6404726406752843
2216	2	-1.527229364680828
2216	3	-2.0038918668739285
2216	4	-1.5754364706794348
2216	5	-1.453080766015404
2216	6	-1.885140847468275
2216	7	-3.3687482921532137
2216	8	-2.43944858011643
2216	9	-2.5110560546138174
2216	10	-2.5688815280564015
2216	11	-3.142134092045108
2216	12	-2.4139741467206193
2216	13	-2.8985663471973915
2216	14	-2.918376493465935
2216	15	-3.130698927170137
2216	16	-2.712717896122207
2216	17	-3.039006188022114
2216	18	-2.801142345970625
2216	19	-3.016415099117083
2216	20	-3.5591021140704378
2216	21	-3.3945973658968285
2216	22	-3.7084608892647175
2216	23	-2.8370083753065813
2216	24	-3.207104123374066
2216	25	-2.8925823140892604
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54428.0
3	0.0
4	909.0
5	7705.0
6	8896.0
7	6888.0
8	5701.0
9	4957.0
10	4356.0
11	4012.0
12	3606.0
13	3401.0
14	3400.0
15	3552.0
16	3561.0
17	3712.0
18	4194.0
19	4963.0
20	6164.0
21	8266.0
22	10482.0
23	12858.0
24	15393.0
25	17911.0
26	20502.0
27	22854.0
28	25600.0
29	31274.0
30	39359.0
31	50146.0
32	63782.0
33	82955.0
34	107142.0
35	139179.0
36	189685.0
37	294786.0
38	955459.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.297649523761486	19.551856582576534	13.308961427112177	27.841532466549808
2	13.677765041716228	20.83219136315012	39.99060321677812	25.499440378355537
3	16.69741921605301	25.191108342881623	30.364647229255304	27.746825211810062
4	11.460841068486491	17.514652525816356	38.083422613956586	32.94108379174057
5	12.564142631145927	38.270370168559744	36.35731549100551	12.808171709288821
6	31.424827970133144	37.962638508927355	18.071445879615574	12.54108764132392
7	27.284685392788028	31.77903554937924	22.98872462229713	17.947554435535604
8	24.716904152252564	37.320737485736004	20.711141813164396	17.251216548847037
9	26.214549560826082	14.85735177317482	20.108303151570382	38.81979551442871
10	14.296733116264946	29.13532623558453	35.19202082198545	21.375919826165077
11	34.06044595260544	22.222337234499783	25.16263611140489	18.554580701489883
12	22.960465760021965	24.81634931101454	32.129339775716836	20.093845153246658
13	28.733778654297776	21.945229301891427	25.93011983340519	23.390872210405604
14	20.742749383405044	21.967613012403913	27.761760311087908	29.52787729310314
15	22.680688906201407	30.97706061346623	23.335084087980597	23.00716639235177
16	22.10136887274043	28.048725045676353	27.597115424001252	22.252790657581958
17	21.661433327255907	28.566695253079793	27.83650372411185	21.93536769555245
18	21.60444711668281	27.56671395088442	29.45076200797111	21.378076924461663
19	23.382175964888752	27.078767181856065	27.467251974152617	22.07180487910256
20	23.508595222536062	27.397148067537618	27.756249966245594	21.338006743680722
21	22.80475077597718	26.862739009296426	27.220878860253002	23.111631354473396
22	23.07174417673177	27.977789718789182	27.16020546940326	21.790260635075793
23	22.12378906211325	27.705916820504417	27.632920769131765	22.53737334825057
24	22.94264226923314	27.073131545514318	28.110603018761616	21.873623166490926
25	22.709252280970365	27.32695431218499	27.691810559183715	22.27198284766093
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	125.0
1	125.0
2	230.5
3	336.0
4	336.0
5	336.0
6	822.5
7	1309.0
8	1309.0
9	1309.0
10	2086.5
11	2864.0
12	2864.0
13	2864.0
14	5817.0
15	8770.0
16	8770.0
17	8770.0
18	16849.0
19	24928.0
20	24928.0
21	24928.0
22	40719.5
23	56511.0
24	56511.0
25	56511.0
26	81749.0
27	106987.0
28	106987.0
29	106987.0
30	137438.5
31	167890.0
32	167890.0
33	167890.0
34	193029.5
35	218169.0
36	218169.0
37	218169.0
38	236981.0
39	255793.0
40	255793.0
41	255793.0
42	267845.5
43	279898.0
44	279898.0
45	279898.0
46	285582.5
47	291267.0
48	291267.0
49	291267.0
50	280050.5
51	268834.0
52	268834.0
53	268834.0
54	239077.0
55	209320.0
56	209320.0
57	209320.0
58	179027.0
59	148734.0
60	148734.0
61	148734.0
62	120832.0
63	92930.0
64	92930.0
65	92930.0
66	71791.5
67	50653.0
68	50653.0
69	50653.0
70	36764.5
71	22876.0
72	22876.0
73	22876.0
74	15952.5
75	9029.0
76	9029.0
77	9029.0
78	6195.0
79	3361.0
80	3361.0
81	3361.0
82	2226.0
83	1091.0
84	1091.0
85	1091.0
86	685.0
87	279.0
88	279.0
89	279.0
90	177.0
91	75.0
92	75.0
93	75.0
94	42.0
95	9.0
96	9.0
97	9.0
98	4.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0774964244535872
2	4.500373080928409E-5
3	0.0
4	0.026012156407766204
5	0.06305022686380701
6	0.08028665576376282
7	0.029207421295225373
8	0.06354526790270915
9	0.04725391734974829
10	0.11075418152164815
11	0.002385197732892057
12	0.013186093127120237
13	0.0054004476971140905
14	0.0071105894678668855
15	0.006255518582490489
16	0.019306600517182873
17	0.03163762275892672
18	6.300522313299773E-4
19	0.0032402686182684544
20	0.004770395465784114
21	4.500373080928409E-5
22	1.8001492323713636E-4
23	0.0
24	4.500373080928409E-5
25	0.003015249964222034
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2222038.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.02298100870658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.62114043914626	57.969767073184585
2	12.576325314650441	17.864162279634684
3	3.1982288388367817	6.814432382466075
4	1.1302896976085866	3.2110617491036546
5	0.5070849022574101	1.8007340691414937
6	0.26343045476150434	1.1225769711384759
7	0.15463644865202106	0.7687919089106426
8	0.10489708972468144	0.5960083209107714
9	0.06974088408183046	0.44578849371068274
>10	0.33730385848733735	4.093362430303471
>50	0.020239318864502233	0.991101357697847
>100	0.013767752240511594	1.9271223199047134
>500	0.0018377178251890384	0.8911379049363476
>1k	0.0010772828630413035	1.5039527389566292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3280	0.1476122370544518	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3111	0.14000660654768282	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3037	0.1366763304677958	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2974	0.13384109542681089	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2938	0.13222096111767664	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.500373080928409E-5	0.0
10	0.0	0.0	0.0	1.3501119242785226E-4	0.0
11	0.0	0.0	0.0	1.3501119242785226E-4	0.0
12	0.0	0.0	0.0	1.3501119242785226E-4	0.0
13	0.0	0.0	0.0	1.3501119242785226E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACAC	55	1.9504577E-4	13.826756	3
TAGGACC	695	0.0	11.90886	4
AACCGGC	65	7.999253E-4	11.696925	7
TCCAACG	110	5.0360177E-7	11.216036	18
GGCGAGG	850	0.0	11.165285	19
TGTAGGA	2110	0.0	10.990864	2
GTAGGAC	2040	0.0	10.71743	3
CCAACGA	80	3.807108E-4	10.676804	19
AGGACGT	1930	0.0	10.597828	5
GGACGTG	1900	0.0	10.362027	6
TGGCGAG	1920	0.0	10.330796	18
GACGTGG	920	0.0	10.330166	7
ATAAGAC	240	0.0	10.298052	3
TAAAGGT	280	0.0	10.192928	4
GGACCTG	1225	0.0	10.170989	6
GGCGTGC	150	2.4343535E-8	10.136422	8
GTACCGT	85	6.5252656E-4	10.070512	6
GCACCTA	95	1.6253816E-4	10.0116205	6
TATGTCG	105	4.1374395E-5	9.945554	16
CTGTAGG	2200	0.0	9.895218	1
>>END_MODULE