FastQCFastQC Report
Thu 26 May 2016
SRR1512965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327720
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41800.17957486295602565No Hit
GGTATCAACGCAGAGTACTTTTTTT26340.11315793995841425No Hit
TATCAACGCAGAGTACTTTTTTTTT26190.112513532555462No Hit
GTCCTACAGTGGACATTTCTAAATT25680.11032254738542437No Hit
GTCCTAAAGTGTGTATTTCTCATTT24850.10675682642242194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGA500.001505510313.29345710
TAGGACC5700.011.497064
TACGCAG752.0800074E-411.396845
GCCGGTT752.0841636E-411.39439111
ATAGCAC2001.8189894E-1210.4473283
AGGACGT13200.010.43271355
GGACGTG12800.010.0137416
GACGTGA6650.09.8514467
GTATAGG2054.0017767E-119.7577441
TGTAGGA16000.09.735222
GTCCTAA10100.09.7140391
GTATTAA3050.09.6815721
GTAGGAC15500.09.6813963
AGGACCT11000.09.5837075
GGCGAGG6250.09.57108319
TAGACTC1804.2036845E-99.4973675
GACCGTG1002.7722056E-49.4945117
TAGGACG14250.09.4642474
GTATCAA25300.09.4124681
GTCTAGA2750.09.3522281
Produced by FastQC (version 0.11.5)