##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512965_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2327720
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.887344697815887	31.0	30.0	34.0	22.0	34.0
2	28.91473287165123	31.0	30.0	34.0	16.0	34.0
3	29.05764396061382	31.0	30.0	34.0	22.0	34.0
4	32.460391713780005	35.0	33.0	37.0	23.0	37.0
5	32.557793033526366	35.0	33.0	37.0	25.0	37.0
6	32.56650370319454	37.0	33.0	37.0	25.0	37.0
7	32.37988632653412	36.0	33.0	37.0	22.0	37.0
8	32.38698039283075	36.0	33.0	37.0	22.0	37.0
9	33.684734418229	38.0	34.0	39.0	21.0	39.0
10	33.53277499011909	38.0	34.0	39.0	19.0	39.0
11	33.62880629972677	38.0	34.0	39.0	21.0	39.0
12	33.52460605227433	38.0	34.0	39.0	18.0	39.0
13	33.535926142319525	38.0	34.0	39.0	18.0	39.0
14	34.53328450157235	39.0	34.0	41.0	18.0	41.0
15	34.47600613475848	39.0	34.0	41.0	17.0	41.0
16	34.37245716838795	39.0	34.0	41.0	17.0	41.0
17	34.32934803155019	39.0	34.0	41.0	17.0	41.0
18	34.029198958637636	39.0	33.0	41.0	11.0	41.0
19	34.26612479164161	39.0	34.0	41.0	18.0	41.0
20	34.33729056759404	39.0	34.0	41.0	18.0	41.0
21	34.27046208306841	39.0	34.0	41.0	16.0	41.0
22	34.194004003917996	39.0	34.0	41.0	10.0	41.0
23	33.92812795353393	39.0	34.0	41.0	8.0	41.0
24	33.93999364184696	39.0	34.0	41.0	2.0	41.0
25	33.859446582922345	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5664494646032914
1101	2	0.8462763305743799
1101	3	0.9466280903501065
1101	4	0.8109118355381284
1101	5	0.7809989019423966
1101	6	0.7647739556020312
1101	7	0.8066272231409002
1101	8	0.6559833742899457
1101	9	0.5965470456538498
1101	10	0.6711561178578265
1101	11	0.7911943660705703
1101	12	1.0134623498597506
1101	13	0.8043797998494284
1101	14	0.7597630715075425
1101	15	0.49041445896767755
1101	16	1.0359223343938737
1101	17	1.102076971924923
1101	18	1.2801481039491307
1101	19	0.8278203056701656
1101	20	0.9561132749507593
1101	21	0.9690618267630171
1101	22	0.9903531855739658
1101	23	1.2125500839180532
1101	24	0.8383065753484473
1101	25	0.9670847673665435
1102	1	0.67831941370385
1102	2	0.6495380396588928
1102	3	0.7043409716105522
1102	4	0.8236318727933281
1102	5	0.7421970135610749
1102	6	0.6150151968351025
1102	7	0.7014856650259915
1102	8	0.7109753452811489
1102	9	0.7768981456886479
1102	10	0.9025065716643823
1102	11	0.8559519760697185
1102	12	0.8099041622369185
1102	13	0.7764767375486201
1102	14	0.7827510399890159
1102	15	0.9451924549577484
1102	16	0.9183560304608349
1102	17	0.6689722297769194
1102	18	1.1470181606307435
1102	19	1.2328298235017883
1102	20	1.0782014454125601
1102	21	0.9008821500848825
1102	22	1.0652708984354362
1102	23	1.2913310694247855
1102	24	1.299233013898771
1102	25	1.065405934148579
1103	1	0.49822770152794504
1103	2	0.6462970165203465
1103	3	0.6675800332471624
1103	4	0.7549697367177188
1103	5	0.23659931760823127
1103	6	0.7218888258768175
1103	7	0.590645017921716
1103	8	0.5533543122394065
1103	9	0.8713700591337883
1103	10	0.8283498380913628
1103	11	0.8921361850223022
1103	12	1.081965198436194
1103	13	0.9859216085798721
1103	14	1.2338598866019552
1103	15	1.3165456760070597
1103	16	1.200174342755865
1103	17	1.1542985208398875
1103	18	1.5083591103325702
1103	19	1.441547742778809
1103	20	1.1759442829403994
1103	21	1.1132762110397536
1103	22	1.2080177666105456
1103	23	1.4078098385584
1103	24	1.265745630901165
1103	25	1.2187470656481523
1104	1	0.6115091518074713
1104	2	0.6340504050294022
1104	3	0.5978149651712314
1104	4	0.9492748307116514
1104	5	0.5770744497265667
1104	6	0.691948587816853
1104	7	0.7770373582331587
1104	8	0.7092645352706839
1104	9	0.9677048482971458
1104	10	0.951164035138774
1104	11	0.923572470703661
1104	12	0.8449205569019966
1104	13	0.8841315726662629
1104	14	1.1772031684990978
1104	15	1.2646294603184316
1104	16	1.1766945562148834
1104	17	1.140981556774662
1104	18	1.2534559735950879
1104	19	1.10366605777903
1104	20	1.2437683227158374
1104	21	1.1546116704964149
1104	22	1.0565081955277051
1104	23	1.326324280439266
1104	24	1.253215379505896
1104	25	0.875230448253923
1105	1	0.6930877814822587
1105	2	0.7623857821236761
1105	3	0.8014246390506479
1105	4	1.0393732068436279
1105	5	0.6693425119733547
1105	6	0.566440238674943
1105	7	0.8197059626879692
1105	8	0.8725732619552176
1105	9	0.7865015486227875
1105	10	0.8847094693224946
1105	11	0.8037649141838727
1105	12	0.8204194150743049
1105	13	0.9169107032468631
1105	14	1.0503919363150445
1105	15	1.1961219725450931
1105	16	1.1807323912179655
1105	17	1.0656852114384563
1105	18	1.1486816471142731
1105	19	1.0278820823398718
1105	20	1.079849534361358
1105	21	1.1090418959544976
1105	22	0.8591477331602775
1105	23	1.1516677700805218
1105	24	1.1203946749848157
1105	25	1.190317052866341
1106	1	0.5428971747330884
1106	2	0.6731828965977158
1106	3	0.7485177855240863
1106	4	0.7283834735857724
1106	5	0.7498501885562234
1106	6	0.7293635372313787
1106	7	0.6264755203354468
1106	8	0.834950896714588
1106	9	0.9892668890456378
1106	10	0.926345709554063
1106	11	0.9658002347814687
1106	12	1.0222742427502425
1106	13	0.9619652562130057
1106	14	1.2046355856630129
1106	15	1.3008566924832792
1106	16	1.2764596344353976
1106	17	1.2383462211579257
1106	18	1.3713344745594966
1106	19	0.9558415841064374
1106	20	1.0474031953254723
1106	21	0.9161580601043298
1106	22	1.0781622654158767
1106	23	1.4064975921898295
1106	24	1.2788469642778395
1106	25	1.3731305211580107
1107	1	0.5739461441386595
1107	2	0.6604734113198631
1107	3	0.7628626830657659
1107	4	0.8385478523039325
1107	5	0.6925591051854667
1107	6	0.5614710539579875
1107	7	0.7025339713713663
1107	8	0.5921611987546029
1107	9	0.7838392173354691
1107	10	0.6766277116895552
1107	11	0.6060511470169416
1107	12	0.9291470446647097
1107	13	0.8237711074939469
1107	14	0.9935696081511409
1107	15	1.1492685656548574
1107	16	1.092175647240488
1107	17	1.0157584259279133
1107	18	1.3456158495956458
1107	19	1.3374790368597047
1107	20	1.161353882691749
1107	21	1.100754766277099
1107	22	1.0858906758179572
1107	23	1.1421486355694128
1107	24	1.152911308869534
1107	25	1.1552275446940996
1108	1	0.5950064130125412
1108	2	0.525680133403462
1108	3	0.4718644742963072
1108	4	0.639869151028897
1108	5	0.5309015839693174
1108	6	0.6883358592356146
1108	7	0.6607621556859158
1108	8	0.705092280153842
1108	9	0.8734199700072267
1108	10	0.7767360107422405
1108	11	0.7394082756767446
1108	12	0.7715373909495611
1108	13	0.8348871038688941
1108	14	1.01139044427957
1108	15	0.9851444791646813
1108	16	0.8896987888979595
1108	17	0.9266406395055355
1108	18	1.0615803988479797
1108	19	1.042318050902054
1108	20	0.9789968676106326
1108	21	0.8310919737084603
1108	22	0.8115255333536098
1108	23	0.9613239714842976
1108	24	1.0307984702187127
1108	25	1.2019276105934011
1109	1	0.18913304741733583
1109	2	0.28338636616821944
1109	3	0.4507888937674096
1109	4	0.5191649218146139
1109	5	0.4394430218759453
1109	6	0.34407144720405825
1109	7	0.3911379719196191
1109	8	0.49176437744974066
1109	9	0.7332498261879508
1109	10	0.7848101969526127
1109	11	0.28565796718600467
1109	12	0.6385987469258154
1109	13	0.7247039736327494
1109	14	0.5311607513157881
1109	15	0.7768142009328614
1109	16	0.746718920377603
1109	17	0.553869596860487
1109	18	-0.9730420979061662
1109	19	0.3686268861960187
1109	20	0.5772527325113614
1109	21	0.725062440265674
1109	22	0.9440899646993657
1109	23	0.49033789797825733
1109	24	0.7433912856929652
1109	25	0.745371750223164
1110	1	0.24126491641390047
1110	2	0.29447102296503047
1110	3	0.33069580587200065
1110	4	0.21297399105102954
1110	5	0.2579668119743985
1110	6	0.3174512613773288
1110	7	0.3690995576323388
1110	8	0.22533363028241382
1110	9	0.6100423701634625
1110	10	0.7618021663910994
1110	11	0.4817857290504435
1110	12	0.08259913622183745
1110	13	0.28316892856049236
1110	14	0.47796068481597587
1110	15	0.5240750035275425
1110	16	0.6015738499271563
1110	17	0.32610724432927896
1110	18	0.7804310970219817
1110	19	0.6693767691261883
1110	20	0.566799572424614
1110	21	0.5866476648844525
1110	22	0.6763377261733226
1110	23	0.6395699110084294
1110	24	0.7151127461683231
1110	25	0.7096823871451718
1111	1	0.15949626501269876
1111	2	0.506584379610679
1111	3	0.5287442186245279
1111	4	0.42460378984432623
1111	5	0.4020035172105807
1111	6	0.5491878576082243
1111	7	0.4671320018331713
1111	8	0.38120433105979856
1111	9	0.6352328342520792
1111	10	0.6620260950739549
1111	11	0.5165941462777042
1111	12	0.5628413741331855
1111	13	0.5577810860889301
1111	14	0.47486223972786945
1111	15	0.7014253987122956
1111	16	0.5531434611534323
1111	17	0.6213568363342645
1111	18	0.8838235582716791
1111	19	0.57351284539034
1111	20	0.4498414427936268
1111	21	0.7021486272774737
1111	22	0.530110362923601
1111	23	0.8907992090798942
1111	24	0.9385338888259369
1111	25	0.3740501431858405
1112	1	-0.10194507786418328
1112	2	0.05871357192755511
1112	3	0.11098431084605309
1112	4	0.20900780054137869
1112	5	0.061217763117277
1112	6	0.16782720122695594
1112	7	-0.09271318517260596
1112	8	0.21501561227344013
1112	9	0.2698333696916535
1112	10	0.18906608720782714
1112	11	0.34637058906429274
1112	12	0.36624750949145835
1112	13	0.1173177184256815
1112	14	0.26296274269766684
1112	15	0.31610849178757405
1112	16	0.5351700232710641
1112	17	0.45783526263940644
1112	18	0.3665203187411663
1112	19	0.30314359607339725
1112	20	0.17484739558732798
1112	21	0.2688640536534379
1112	22	0.2450318245701837
1112	23	0.46042378133176953
1112	24	0.25187354000873086
1112	25	0.47790568236586495
1113	1	-0.44645106454717265
1113	2	-0.22030118825677292
1113	3	-0.32570443709049357
1113	4	-0.1788347755025086
1113	5	-0.23781651901396117
1113	6	-0.18533725944548962
1113	7	-0.3146399714734045
1113	8	-0.08371489243926789
1113	9	-0.2663667608338116
1113	10	-0.4633067606503758
1113	11	-0.36080454398495476
1113	12	-0.2227609092592786
1113	13	-0.2185883743270267
1113	14	-0.15749691170842084
1113	15	-0.5018226937050443
1113	16	-0.46313321668964136
1113	17	-0.5155773528322669
1113	18	-0.6467719516768966
1113	19	-0.5309171537229247
1113	20	-0.6859331837012093
1113	21	-0.5454354525394152
1113	22	-0.34611142500219927
1113	23	-0.726686899433922
1113	24	-0.09228857319580897
1113	25	-0.059703995287407
1114	1	-1.5574489047418787
1114	2	-1.2684273274258082
1114	3	-0.9982292444077849
1114	4	-1.1748487761824151
1114	5	-1.1406134415028255
1114	6	-1.6470105814244853
1114	7	-1.7305297622073823
1114	8	-1.222278015863882
1114	9	-1.4410991530084516
1114	10	-1.5713725707239377
1114	11	-2.292975466838019
1114	12	-1.6999110036062959
1114	13	-1.5621396275609598
1114	14	-1.7933473117882528
1114	15	-1.7327132652613528
1114	16	-2.4534167707673618
1114	17	-4.249526661959543
1114	18	-3.310498554490902
1114	19	-2.3401058821438987
1114	20	-1.5658290383521205
1114	21	-2.5658439494648917
1114	22	-2.707059794323829
1114	23	-2.6675177620424577
1114	24	-2.192281751571148
1114	25	-2.0627999417201757
1115	1	-1.285939591527466
1115	2	-0.9190637608918486
1115	3	-0.9754637509510395
1115	4	-1.0861885798494022
1115	5	-0.8455383258798008
1115	6	-0.886943630512274
1115	7	-2.000705416199441
1115	8	-1.4831547745233102
1115	9	-1.1747387539683132
1115	10	-0.9319000572604565
1115	11	-1.7159888646762056
1115	12	-1.3856765342075832
1115	13	-1.1133562814727753
1115	14	-1.3138325272677136
1115	15	-2.5421803318036744
1115	16	-1.376017183667237
1115	17	-1.353322267164657
1115	18	-1.3105215217274306
1115	19	-1.4542928859204522
1115	20	-1.2854922252461662
1115	21	-1.9414977713973585
1115	22	-2.0060838568990604
1115	23	-1.551684489435253
1115	24	-1.3210581961465238
1115	25	-1.71002630765404
1116	1	-1.5741286472854874
1116	2	-2.152628184776699
1116	3	-1.7187139793846704
1116	4	-1.4275102943153541
1116	5	-1.754330367196065
1116	6	-1.635225955766522
1116	7	-1.2069473472569499
1116	8	-1.961344604817853
1116	9	-2.085301301070583
1116	10	-2.8780082981241044
1116	11	-1.6720932711059788
1116	12	-1.5513882806754182
1116	13	-2.2307089457076437
1116	14	-2.011697647811964
1116	15	-1.7100053881829638
1116	16	-1.521201185267124
1116	17	-1.924007262070063
1116	18	-1.7168288990716505
1116	19	-2.0571509256709746
1116	20	-2.3118829520086663
1116	21	-2.752340517671623
1116	22	-1.937662571324168
1116	23	-1.9888502485828106
1116	24	-2.655233411059921
1116	25	-2.3497380789835383
1201	1	0.28258276958572637
1201	2	0.23988512342641144
1201	3	0.4797573573333267
1201	4	0.5146063097095563
1201	5	0.5570295710010242
1201	6	0.6123109964746618
1201	7	0.43402322247537484
1201	8	-0.019797404486489256
1201	9	0.336766007077685
1201	10	0.568012783119471
1201	11	0.2725045214603625
1201	12	-0.27173798938260774
1201	13	0.11509193915084381
1201	14	0.6247390863648832
1201	15	0.2468003607578666
1201	16	0.7253228974213144
1201	17	0.8583181973181482
1201	18	1.1380948328141258
1201	19	0.6928571797850864
1201	20	0.6899382575841599
1201	21	0.5733654528477459
1201	22	0.8607239687224109
1201	23	1.0764884025178532
1201	24	0.8771446327042511
1201	25	0.7713436130592726
1202	1	0.7430390977952968
1202	2	0.7904661541782545
1202	3	0.5643994035884177
1202	4	0.879852558768917
1202	5	0.82370619903012
1202	6	0.7375985160650913
1202	7	0.8699615668330054
1202	8	0.8433208533056487
1202	9	1.2070896412515353
1202	10	1.3552002142952944
1202	11	0.9892778793457495
1202	12	1.214929535720728
1202	13	1.2716648851341645
1202	14	1.3177406609759643
1202	15	1.001534951785608
1202	16	1.2668356731964892
1202	17	1.4243980534024132
1202	18	1.7049046605970304
1202	19	1.4795836452262208
1202	20	1.399262396082726
1202	21	1.3939458516568735
1202	22	1.5016596930996542
1202	23	1.590999930062729
1202	24	1.7637876341682102
1202	25	1.5570465935912594
1203	1	0.5636619712907418
1203	2	0.7718463099941673
1203	3	0.7397624568169334
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2211	17	0.1375672129321046
2211	18	0.1593825966501754
2211	19	-0.1250812164972146
2211	20	-0.22016646905270676
2211	21	0.013680218796700672
2211	22	-0.13878249195441583
2211	23	0.3644624996228245
2211	24	0.10393783085807229
2211	25	0.19045574912153995
2212	1	-0.26936180314235614
2212	2	-0.29269285121884536
2212	3	-0.3364402646391831
2212	4	-0.4542650129482553
2212	5	-0.480131576156964
2212	6	-0.5315927075053537
2212	7	-0.4945833560483379
2212	8	-0.5262941145379081
2212	9	-0.3636668468034614
2212	10	-0.38306726800392
2212	11	-0.6631869296052457
2212	12	-0.34928390109533325
2212	13	-0.5528149905535571
2212	14	-0.4279224874196643
2212	15	-0.6336964457468994
2212	16	-0.45095057932991267
2212	17	-0.4944208044646814
2212	18	-3.3988187039441975
2212	19	-1.7087099997334647
2212	20	-0.8245451169343312
2212	21	-0.9760796829150138
2212	22	-0.5279160438383741
2212	23	-0.6131556456320268
2212	24	-1.030369956644087
2212	25	-0.6710493943543696
2213	1	-0.26707493134264126
2213	2	-0.09834147457291209
2213	3	-0.47287412722713285
2213	4	-0.5780028981389975
2213	5	-0.3463528852778168
2213	6	-0.36046091923720525
2213	7	-0.19602877139841013
2213	8	-0.6410256402305805
2213	9	-0.4023176718673298
2213	10	-0.5076567731921102
2213	11	-0.5398365388675828
2213	12	-0.6269559250254133
2213	13	-0.500561558444069
2213	14	-0.49857010250860156
2213	15	-0.7506577008612112
2213	16	-1.3301302648247386
2213	17	-0.8651298862197621
2213	18	-0.595810838005363
2213	19	-0.5207713657937205
2213	20	-1.1608104352467947
2213	21	-0.8118557977539851
2213	22	-0.7291506133738821
2213	23	-1.0435545376729394
2213	24	-0.8457761742349064
2213	25	-1.0638501460156533
2214	1	-1.8151843842954243
2214	2	-3.882097797432209
2214	3	-2.2193754345257872
2214	4	-1.9349031778878576
2214	5	-1.7847263466838967
2214	6	-1.8191113449136154
2214	7	-2.0066930125419447
2214	8	-1.5888897165887599
2214	9	-1.7926972086837054
2214	10	-1.9279455994430243
2214	11	-1.751259931502421
2214	12	-2.369232184141449
2214	13	-2.130622946865234
2214	14	-3.2429801441560606
2214	15	-3.3689967961096343
2214	16	-2.786355014702206
2214	17	-3.2270448678783445
2214	18	-2.6480089721631437
2214	19	-4.625815386794461
2214	20	-2.968168130218178
2214	21	-2.777480327597054
2214	22	-2.9898111486026266
2214	23	-2.9557167240756463
2214	24	-3.295135096776253
2214	25	-3.0056386594201854
2215	1	-1.2399458639113377
2215	2	-2.9088328890737856
2215	3	-1.6152248199885086
2215	4	-1.2436472961139842
2215	5	-1.2156468566670497
2215	6	-1.5678285684539368
2215	7	-1.9100700250588858
2215	8	-2.047939618768279
2215	9	-1.8738705603253543
2215	10	-3.2173454471226712
2215	11	-2.300508081859764
2215	12	-2.5201981354937715
2215	13	-1.8278425863389494
2215	14	-3.204877574521216
2215	15	-3.031232474029583
2215	16	-2.6095090281348163
2215	17	-2.820168906764689
2215	18	-1.6823144283346707
2215	19	-2.497567127636863
2215	20	-2.5441679454572395
2215	21	-2.2534972378264158
2215	22	-1.8993570893941154
2215	23	-2.3044245254220392
2215	24	-2.1527774091176823
2215	25	-2.1154426670444906
2216	1	-2.444009220216742
2216	2	-2.5817156236584644
2216	3	-2.955481794667172
2216	4	-3.739541744702475
2216	5	-2.8039619945886187
2216	6	-2.871472964482706
2216	7	-2.6874561443128044
2216	8	-3.3168722785317826
2216	9	-3.618785895344036
2216	10	-3.0109027229276535
2216	11	-3.691256200796335
2216	12	-3.9335540062783316
2216	13	-4.578804978035354
2216	14	-3.920759317432502
2216	15	-3.89009396933044
2216	16	-3.0602679728269635
2216	17	-3.130088425198309
2216	18	-3.135498486658207
2216	19	-3.585646096720847
2216	20	-3.4151055062766105
2216	21	-3.8224536028712457
2216	22	-4.4287133326270975
2216	23	-4.32793894166586
2216	24	-4.327237966638201
2216	25	-4.2478231826292046
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	127326.0
3	7.0
4	972.0
5	5908.0
6	7433.0
7	6578.0
8	5860.0
9	5414.0
10	4879.0
11	4689.0
12	4550.0
13	4533.0
14	4481.0
15	4511.0
16	4687.0
17	4936.0
18	5664.0
19	6824.0
20	8632.0
21	11014.0
22	13768.0
23	16766.0
24	19207.0
25	21899.0
26	24353.0
27	26977.0
28	30288.0
29	36028.0
30	44370.0
31	55098.0
32	69737.0
33	87776.0
34	114816.0
35	151095.0
36	206079.0
37	318548.0
38	862017.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34510971262415	18.668608030243192	12.523562818949593	25.46271943818306
2	14.872502801109011	20.387045144808845	40.95842410702966	23.782027947052487
3	18.298116300228163	25.184615986285365	29.900849085268238	26.616418628218234
4	12.311075545714905	17.073897138456353	38.65843008763757	31.956597228191175
5	13.09392008746987	38.045612237530214	35.92629872474153	12.934168950258387
6	31.357790013087982	37.54841393083736	18.372716155794247	12.721079900280408
7	27.35352578783142	32.00046576818666	22.86225722262946	17.783751221352464
8	25.00240633346969	36.54833808297725	20.98950151083366	17.459754072719395
9	26.447794491729343	15.145423982839937	20.317291033591374	38.08949049183934
10	14.797862914764949	28.86083422636363	34.94226312067038	21.399039738201044
11	33.92284436530635	22.653671900780992	24.914063359366406	18.509420374546252
12	22.87959822677345	24.980674889989743	31.89044907731462	20.249277805922187
13	28.632087875824354	22.129056874880764	25.863109100565296	23.375746148729593
14	20.97337268444113	22.038611412394225	27.92765538330374	29.060360519860907
15	23.05087178447023	30.395871883307134	23.585063766992427	22.968192565230208
16	22.41114681347126	28.04660060345487	27.358024394289266	22.18422818878461
17	21.78956007597047	28.398819191997323	27.800594700973697	22.011026031058517
18	21.659679622372764	27.71191645134987	29.18521659550604	21.44318733077132
19	23.23819591261452	27.211708692139773	27.70153319926434	21.84856219598137
20	23.439539053795247	27.38949980083473	27.85765634688359	21.313304798486428
21	23.10285141659834	26.818682631436914	27.353119129019337	22.725346822945404
22	23.084174835334018	27.81977469903798	27.290875598926988	21.805174866701012
23	22.509067643048756	27.573283442855477	27.57452955894795	22.343119355147813
24	22.827880404518176	27.074453274850985	28.095846195095458	22.00182012553538
25	22.766843902300682	27.230191189323577	27.787078327722924	22.215886580652814
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	381.0
1	381.0
2	616.0
3	851.0
4	851.0
5	851.0
6	1641.5
7	2432.0
8	2432.0
9	2432.0
10	3686.5
11	4941.0
12	4941.0
13	4941.0
14	8316.5
15	11692.0
16	11692.0
17	11692.0
18	20803.0
19	29914.0
20	29914.0
21	29914.0
22	45951.0
23	61988.0
24	61988.0
25	61988.0
26	86517.5
27	111047.0
28	111047.0
29	111047.0
30	141319.5
31	171592.0
32	171592.0
33	171592.0
34	196167.0
35	220742.0
36	220742.0
37	220742.0
38	239338.5
39	257935.0
40	257935.0
41	257935.0
42	271658.5
43	285382.0
44	285382.0
45	285382.0
46	293010.5
47	300639.0
48	300639.0
49	300639.0
50	290957.5
51	281276.0
52	281276.0
53	281276.0
54	252876.5
55	224477.0
56	224477.0
57	224477.0
58	193686.5
59	162896.0
60	162896.0
61	162896.0
62	132554.0
63	102212.0
64	102212.0
65	102212.0
66	78965.0
67	55718.0
68	55718.0
69	55718.0
70	40724.5
71	25731.0
72	25731.0
73	25731.0
74	18029.5
75	10328.0
76	10328.0
77	10328.0
78	7059.0
79	3790.0
80	3790.0
81	3790.0
82	2518.5
83	1247.0
84	1247.0
85	1247.0
86	810.5
87	374.0
88	374.0
89	374.0
90	233.0
91	92.0
92	92.0
93	92.0
94	59.0
95	26.0
96	26.0
97	26.0
98	21.5
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3603526197308955
2	0.04137095526953414
3	0.03707490591651917
4	0.03711786641004932
5	0.04192944168542608
6	0.01692643445087897
7	0.016410908528517176
8	0.02268314058391903
9	0.01958998504974825
10	0.018816696166205557
11	0.018215249256783462
12	0.018773735672675408
13	0.01873077517914526
14	0.027322873885175195
15	0.028096162768717885
16	0.01950406406268795
17	0.021480246765074835
18	0.10585465605828878
19	0.024057876376883818
20	0.021780970219785884
21	0.025604454143969203
22	0.01860189369855481
23	0.021007681336243193
24	0.017527881360301065
25	0.021394325778014537
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2327720.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.96076069620187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.91225483140467	57.415951524814105
2	12.678566651647177	17.993614682767614
3	3.521308232201812	7.496241324085154
4	1.2997346159235117	3.689206281964727
5	0.5703714346568493	2.023699544131702
6	0.30674658433050417	1.306018257903253
7	0.17085434066564087	0.8486767787317326
8	0.12028171450506832	0.6828225567298422
9	0.07566938162377763	0.4832601193291014
>10	0.3145600791983625	3.7839031260755283
>50	0.015919391014699753	0.7711722217463448
>100	0.011488037018614942	1.6502248647537965
>500	0.001395357665210092	0.6641366518953083
>1k	8.493481440334602E-4	1.1910720650718303
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4180	0.17957486295602565	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2634	0.11315793995841425	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2619	0.112513532555462	No Hit
GTCCTACAGTGGACATTTCTAAATT	2568	0.11032254738542437	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2485	0.10675682642242194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.592098706029934E-5	0.0
5	0.0	0.0	0.0	8.592098706029934E-5	0.0
6	0.0	0.0	0.0	8.592098706029934E-5	0.0
7	0.0	0.0	0.0	8.592098706029934E-5	0.0
8	0.0	0.0	0.0	8.592098706029934E-5	0.0
9	0.0	0.0	0.0	2.5776296118089803E-4	0.0
10	4.296049353014967E-5	0.0	0.0	3.007234547110477E-4	0.0
11	4.296049353014967E-5	0.0	0.0	3.007234547110477E-4	0.0
12	4.296049353014967E-5	0.0	0.0	3.436839482411974E-4	4.296049353014967E-5
13	4.296049353014967E-5	0.0	0.0	3.436839482411974E-4	4.296049353014967E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTCGA	50	0.0015055103	13.293457	10
TAGGACC	570	0.0	11.49706	4
TACGCAG	75	2.0800074E-4	11.39684	5
GCCGGTT	75	2.0841636E-4	11.394391	11
ATAGCAC	200	1.8189894E-12	10.447328	3
AGGACGT	1320	0.0	10.4327135	5
GGACGTG	1280	0.0	10.013741	6
GACGTGA	665	0.0	9.851446	7
GTATAGG	205	4.0017767E-11	9.757744	1
TGTAGGA	1600	0.0	9.73522	2
GTCCTAA	1010	0.0	9.714039	1
GTATTAA	305	0.0	9.681572	1
GTAGGAC	1550	0.0	9.681396	3
AGGACCT	1100	0.0	9.583707	5
GGCGAGG	625	0.0	9.571083	19
TAGACTC	180	4.2036845E-9	9.497367	5
GACCGTG	100	2.7722056E-4	9.494511	7
TAGGACG	1425	0.0	9.464247	4
GTATCAA	2530	0.0	9.412468	1
GTCTAGA	275	0.0	9.352228	1
>>END_MODULE