FastQCFastQC Report
Thu 26 May 2016
SRR1512965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327720
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49730.21364253432543434No Hit
TATCAACGCAGAGTACTTTTTTTTT36750.15787981372330007No Hit
GGTATCAACGCAGAGTACTTTTTTT31750.1363995669582252No Hit
GTCCTACAGTGGACATTTCTAAATT23930.10280446101764817No Hit
GTCCTAAAGTGTGTATTTCTCATTT23710.10185933015998487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG855.296905E-511.182337
AACTGCG700.001485374810.8628357
TAGGACC4950.010.7637634
GTAAGAC2050.010.666193
GTCCTAA8550.010.4514941
GGCGAGG7300.010.40030719
GGTATCA12400.010.1197291
TGTAGGA15950.09.8938212
GGACGTG16050.09.72020156
GTAGGAC15250.09.6003313
TCCAACG1002.7852415E-49.49007518
AGGACGT16750.09.4859965
TTAGGAC11950.09.3874893
TGGCGAG14500.09.35917818
TAAGACT3050.09.3584234
TAATACA2850.09.3474784
ATTATAC2451.8189894E-129.3128043
GACGTGA9600.09.207957
TAGGACG18150.09.1736614
TCTATAC2600.09.1411743
Produced by FastQC (version 0.11.5)