##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512965_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2327720
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.210341879607512	31.0	31.0	34.0	26.0	34.0
2	30.362720602134278	31.0	31.0	34.0	26.0	34.0
3	30.481414431288986	31.0	31.0	34.0	27.0	34.0
4	33.952284639045935	37.0	35.0	37.0	30.0	37.0
5	33.55674136064475	37.0	35.0	37.0	28.0	37.0
6	33.401703813173405	37.0	35.0	37.0	28.0	37.0
7	33.265230354166306	37.0	35.0	37.0	27.0	37.0
8	33.1415531077621	37.0	35.0	37.0	26.0	37.0
9	34.56806660594917	39.0	35.0	39.0	27.0	39.0
10	34.45850789613871	39.0	35.0	39.0	27.0	39.0
11	34.47966851683192	39.0	35.0	39.0	27.0	39.0
12	34.32202541542797	39.0	35.0	39.0	26.0	39.0
13	34.2973957348822	39.0	35.0	39.0	25.0	39.0
14	35.337689240974	40.0	36.0	41.0	25.0	41.0
15	35.34887615348925	40.0	36.0	41.0	25.0	41.0
16	35.32172426236833	40.0	36.0	41.0	25.0	41.0
17	35.298115752753766	40.0	36.0	41.0	25.0	41.0
18	35.223765315415946	40.0	36.0	41.0	25.0	41.0
19	35.20180949598749	40.0	35.0	41.0	25.0	41.0
20	35.176232106954444	40.0	35.0	41.0	25.0	41.0
21	35.12110004639733	40.0	35.0	41.0	24.0	41.0
22	35.076377313422576	40.0	35.0	41.0	24.0	41.0
23	34.969010877596965	40.0	35.0	41.0	23.0	41.0
24	34.95234822057636	40.0	35.0	41.0	23.0	41.0
25	34.857885828192394	40.0	35.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.616652651467323
1101	2	0.6635410454375688
1101	3	0.5681786847709454
1101	4	0.44200102309631717
1101	5	0.6864450387588832
1101	6	0.6390563648876721
1101	7	0.6559750921305749
1101	8	0.6599829760910225
1101	9	0.4691018900268489
1101	10	0.8451614091616833
1101	11	0.8525951411498767
1101	12	0.8774442465757772
1101	13	0.8829332738239941
1101	14	0.9647721625540626
1101	15	0.8764282149909945
1101	16	1.012967739603205
1101	17	0.9724364677751964
1101	18	0.4916061382102299
1101	19	0.8190991563734045
1101	20	0.7322339636513604
1101	21	1.0129740287298858
1101	22	0.8316585311918132
1101	23	0.8369037550167135
1101	24	0.753352784334794
1101	25	0.895552726741883
1102	1	0.43071253033847867
1102	2	0.405595566525065
1102	3	0.35551617246404277
1102	4	0.23229661872807839
1102	5	0.4958475237080435
1102	6	0.46445108223763043
1102	7	0.5386190040446976
1102	8	0.48881262038354834
1102	9	0.6029834168270227
1102	10	0.654852260790598
1102	11	0.6543408230072387
1102	12	0.567683776404543
1102	13	0.764531547383072
1102	14	0.7967206680801411
1102	15	0.7527844472602112
1102	16	0.6773428062230806
1102	17	0.751497633909274
1102	18	0.8630152496575221
1102	19	0.8690360545223399
1102	20	0.8669575904039704
1102	21	0.8148750230013775
1102	22	0.832662621002946
1102	23	0.8521144790915969
1102	24	0.7492399731133261
1102	25	0.8986090178468089
1103	1	0.5208949739393169
1103	2	0.37834025791143944
1103	3	0.5102059561486527
1103	4	0.4747325170840071
1103	5	0.5252798602635025
1103	6	0.5761411553633806
1103	7	0.5468209526294245
1103	8	0.5924404421356471
1103	9	0.6626271889387709
1103	10	0.6902358420625632
1103	11	0.5845807242623593
1103	12	0.7313048525036407
1103	13	0.7344057481308326
1103	14	0.9012675252517042
1103	15	0.7352648860625521
1103	16	0.8454612235302292
1103	17	0.9653192855417458
1103	18	0.9904107338973276
1103	19	0.9894031424335097
1103	20	1.0102182764033927
1103	21	0.6487310248384901
1103	22	0.9120166170847313
1103	23	0.9801527140269002
1103	24	0.9486821410992192
1103	25	1.0492884869017232
1104	1	0.5390105325053121
1104	2	0.5495402240439873
1104	3	0.4564611332824775
1104	4	0.4922716234066371
1104	5	0.5646599447910177
1104	6	0.5741874267686669
1104	7	0.5262398906496344
1104	8	0.4820812354806918
1104	9	0.7502806214284732
1104	10	0.7482926500082385
1104	11	0.7484991883734438
1104	12	0.6777865182177862
1104	13	0.8297873096555293
1104	14	0.9662471695023527
1104	15	0.9795009516054733
1104	16	0.9424105658196211
1104	17	0.7565629030559222
1104	18	0.9720204726710904
1104	19	0.9584377084009503
1104	20	0.9199104824209812
1104	21	0.8221417829284619
1104	22	0.9131180148226647
1104	23	0.7873293210257089
1104	24	0.8740086444965485
1104	25	0.8970846330679976
1105	1	0.5235247637193083
1105	2	0.5681148085083763
1105	3	0.5056697727432535
1105	4	0.403845133004161
1105	5	0.49603126762256267
1105	6	0.5942342178310867
1105	7	0.6556730371525532
1105	8	0.6556886540417679
1105	9	0.7556923761787147
1105	10	0.7447244336156871
1105	11	0.7583045827377859
1105	12	0.7014634013600798
1105	13	0.736804377485484
1105	14	0.9546220709534765
1105	15	0.894482747964588
1105	16	0.8374168407285651
1105	17	0.6312435193129247
1105	18	0.8682846018911263
1105	19	0.8589008444269268
1105	20	0.8671354121897465
1105	21	0.8412305489199667
1105	22	0.8721400839472011
1105	23	0.9461694119682136
1105	24	0.9232272989681931
1105	25	1.04430901938656
1106	1	0.33102831160707424
1106	2	0.35440882847312594
1106	3	0.38821255530669063
1106	4	0.3725582624691981
1106	5	0.47476379310162997
1106	6	0.4109893693104638
1106	7	0.37870171267038444
1106	8	0.518169573246503
1106	9	0.5553323561120251
1106	10	0.486810564794979
1106	11	0.4190057902268478
1106	12	0.6338719141445495
1106	13	0.4209782494717942
1106	14	0.6040805653613646
1106	15	0.6566167923956812
1106	16	0.643526987553436
1106	17	0.500969909427667
1106	18	0.6686172582865808
1106	19	0.6665390645005971
1106	20	0.7861400184077354
1106	21	0.7033718366757995
1106	22	0.7196825848257902
1106	23	0.8040505516919509
1106	24	0.7210306936730504
1106	25	0.8284729653352656
1107	1	0.5691498047046402
1107	2	0.46871792305237747
1107	3	0.4670527779601876
1107	4	0.5210460083181587
1107	5	0.5466653699196797
1107	6	0.5905093951435774
1107	7	0.655255318450088
1107	8	0.7972416957882444
1107	9	0.5382333264470702
1107	10	0.744156836533044
1107	11	0.6648011699153429
1107	12	0.7024563740578813
1107	13	0.7740386551334808
1107	14	0.8571144718466854
1107	15	0.8587354275997399
1107	16	0.7260843446015599
1107	17	0.8613834778215548
1107	18	0.823694153851811
1107	19	1.0110014729381618
1107	20	0.9066738140707713
1107	21	0.9055499043443831
1107	22	0.8603851309291883
1107	23	0.8635215302606909
1107	24	0.9248187996434751
1107	25	1.0307616664987904
1108	1	0.4317524987674304
1108	2	0.3535280603051234
1108	3	0.3656522255655119
1108	4	0.4909726495006055
1108	5	0.4799908355627167
1108	6	0.4835577583682138
1108	7	0.4187047061308249
1108	8	0.5883374372973478
1108	9	0.5413609551725358
1108	10	0.7926040620778423
1108	11	0.594750701344104
1108	12	0.7450271596083056
1108	13	0.7177740954697711
1108	14	0.892673178422065
1108	15	0.804942757211009
1108	16	0.7136430417120465
1108	17	0.7285131994709602
1108	18	0.8465381848777227
1108	19	0.7830019455911383
1108	20	0.7434083706228947
1108	21	1.0015607931094408
1108	22	0.9938029679887279
1108	23	0.9246236842331683
1108	24	0.9922554795254968
1108	25	0.934942293879061
1109	1	0.21623513148402296
1109	2	0.24564460184675596
1109	3	0.29219022327880495
1109	4	0.3915323739036296
1109	5	0.34048297813968276
1109	6	0.10971124547598521
1109	7	0.3442409286065953
1109	8	0.4877750452691103
1109	9	0.5432228532579231
1109	10	0.4248962168156041
1109	11	0.4687743356094032
1109	12	0.46347399666915834
1109	13	0.490368061450539
1109	14	0.6148917245871814
1109	15	0.5752523269215715
1109	16	0.447168742120148
1109	17	0.5585369636898463
1109	18	0.4339788413937029
1109	19	0.7627429713422131
1109	20	0.6371479907292183
1109	21	0.7919785193747231
1109	22	0.8722711788378277
1109	23	0.6644970659235412
1109	24	0.34331056255868475
1109	25	0.5696881422196469
1110	1	0.14826171010746947
1110	2	0.13580640226391694
1110	3	0.1257932436952487
1110	4	0.22435236502317935
1110	5	0.0939852371511023
1110	6	0.24316375387646616
1110	7	0.11390252209931617
1110	8	0.2753736154049591
1110	9	0.2805648903351212
1110	10	0.45395712004744126
1110	11	0.19537767462144728
1110	12	0.37326374132232587
1110	13	0.1526783203176052
1110	14	0.35736575401689663
1110	15	0.5011923096635655
1110	16	0.483807088191881
1110	17	0.4262457613952506
1110	18	0.1868756356475032
1110	19	0.25717235538184724
1110	20	0.38766715068994984
1110	21	0.46522604389874544
1110	22	0.43905164162085697
1110	23	0.454953160521292
1110	24	0.4118495133112603
1110	25	0.3763603682858587
1111	1	-0.37118568355039017
1111	2	-0.18169216396085375
1111	3	-0.16654474413146048
1111	4	-0.17370215769274466
1111	5	0.19118147235104033
1111	6	0.10552959664261152
1111	7	0.24994513717545885
1111	8	0.23486714076233994
1111	9	-0.12212921980774638
1111	10	-0.01461427605558896
1111	11	0.18959733931728806
1111	12	0.16656640972340142
1111	13	0.18270510084801117
1111	14	0.4390422394242677
1111	15	0.508849835293681
1111	16	0.25964094323344256
1111	17	0.5453163297015422
1111	18	0.5031083768456881
1111	19	0.45609497992695935
1111	20	0.42533116409762783
1111	21	0.2595369300530095
1111	22	0.22433092450596348
1111	23	0.3423308911510077
1111	24	0.5025174592330686
1111	25	0.45408087376578266
1112	1	-0.023991017840572226
1112	2	-0.23429358067072314
1112	3	0.048853564724794296
1112	4	-0.03067338359402072
1112	5	0.11694718139756333
1112	6	0.04933940662650116
1112	7	0.08996082540096495
1112	8	0.24879532053560638
1112	9	-0.020817403851616234
1112	10	0.2699840030091849
1112	11	-0.007576138482740191
1112	12	0.18046582530397615
1112	13	0.2791948787363481
1112	14	0.2862383360059084
1112	15	0.19478476878199302
1112	16	-0.05360419703052344
1112	17	0.11305875516137576
1112	18	0.09099190094358534
1112	19	0.1623526960867423
1112	20	0.37068059217082094
1112	21	0.4369296687820494
1112	22	0.281903263332012
1112	23	0.5778699317551172
1112	24	0.3527346032055547
1112	25	0.4071643475263471
1113	1	-0.2325482563879575
1113	2	-0.5931071069770084
1113	3	-0.3345426142060077
1113	4	-0.30312476001213895
1113	5	-0.2793759956891009
1113	6	-0.3199721781489373
1113	7	-0.1454688067248
1113	8	-0.1259923622960315
1113	9	-0.1655106476655206
1113	10	-0.3675137753562936
1113	11	-0.21169145873224693
1113	12	-0.20844555245066942
1113	13	-0.08894467830172204
1113	14	-0.1310326152517831
1113	15	-0.026013427179684356
1113	16	-0.32338861672536723
1113	17	-0.1997409877688554
1113	18	-0.03166621189696173
1113	19	-0.11540216627144417
1113	20	0.09341200117764714
1113	21	-0.14307099948561586
1113	22	-0.28527086020650927
1113	23	-0.15759887223060076
1113	24	-0.4094437929804826
1113	25	-0.4472799168175925
1114	1	-0.6439018076637417
1114	2	-0.4794261413957699
1114	3	-0.47446780748067496
1114	4	-0.29959400547843273
1114	5	-0.36552226259728826
1114	6	-0.8192161617296847
1114	7	-0.6357726565453206
1114	8	-0.9643088642571058
1114	9	-0.7586160992711655
1114	10	-0.8161263640162701
1114	11	-0.6160916039595605
1114	12	-0.7595165475227503
1114	13	-0.5392795862464297
1114	14	-1.82964548579708
1114	15	-1.1156848109152335
1114	16	-1.0792608516418198
1114	17	-1.1615911927840443
1114	18	-1.168963529307156
1114	19	-1.155472023090617
1114	20	-0.6349473811777955
1114	21	-0.7384137748495121
1114	22	-0.806216912430834
1114	23	-0.8007066916729073
1114	24	-1.3259912221582084
1114	25	-1.1613581433755868
1115	1	-1.565677951963771
1115	2	-1.3658673340701846
1115	3	-1.1273553136726129
1115	4	-2.2887538951563045
1115	5	-1.6166923865891718
1115	6	-1.455980809651873
1115	7	-1.171883390382554
1115	8	-1.1781723986085737
1115	9	-1.6326364119488233
1115	10	-1.4323615632627167
1115	11	-1.3539454092762213
1115	12	-1.8873565810038784
1115	13	-1.4541387644787136
1115	14	-2.198156497169265
1115	15	-2.074010618461706
1115	16	-1.5286148343165848
1115	17	-1.5875636780346838
1115	18	-1.4808603026372609
1115	19	-1.447918524315675
1115	20	-1.4361161866801808
1115	21	-1.3993096161634924
1115	22	-1.7418498143298322
1115	23	-2.017357484482069
1115	24	-1.5557436892221972
1115	25	-1.4253764513821068
1116	1	-0.9432605428963363
1116	2	-0.9395365485112066
1116	3	-0.799546529390323
1116	4	-0.959833780743466
1116	5	-0.9810986360470295
1116	6	-1.6046134111916288
1116	7	-0.8359201162541368
1116	8	-1.117244104980827
1116	9	-1.1219357869784545
1116	10	-1.0465338466851648
1116	11	-1.0230095191800856
1116	12	-1.6535097313253004
1116	13	-1.2435581994987146
1116	14	-1.7498022370066835
1116	15	-1.4675688105377418
1116	16	-1.7845311253603597
1116	17	-1.5360137648934185
1116	18	-2.375537542888516
1116	19	-1.760104893374539
1116	20	-1.5966066417811717
1116	21	-1.5665694794489156
1116	22	-3.0044767744570464
1116	23	-2.8612357158668615
1116	24	-2.1473604993693627
1116	25	-2.3614361395976857
1201	1	0.6940076897664973
1201	2	0.6713694326831074
1201	3	0.5964032716271639
1201	4	0.6505382328554674
1201	5	0.750684948790699
1201	6	0.7319063037404021
1201	7	0.7835990423605566
1201	8	0.8065903396270713
1201	9	0.9144041522464335
1201	10	0.7219323488578411
1201	11	0.9004500526798012
1201	12	0.8317489621163574
1201	13	0.805258995742939
1201	14	1.021436722939427
1201	15	1.0029499364447645
1201	16	1.0380815752165944
1201	17	0.8067281465782372
1201	18	0.8237487505683987
1201	19	0.7459697875406732
1201	20	0.9155362871476171
1201	21	0.8331972010466515
1201	22	0.9550199123780558
1201	23	0.9298746610357469
1201	24	0.8992585550029162
1201	25	0.8383017803136426
1202	1	0.6995012472703728
1202	2	0.7217232181469981
1202	3	0.6928998582758972
1202	4	0.7043445893795166
1202	5	0.923690700289022
1202	6	0.9311762828633405
1202	7	0.844746753600937
1202	8	0.8130463188337842
1202	9	1.0244087397734916
1202	10	0.9907609426344948
1202	11	0.9288063303421552
1202	12	0.9232026295274451
1202	13	0.9173921073034492
1202	14	0.9159457309098542
1202	15	1.116580448966701
1202	16	1.0523420879032699
1202	17	1.1361075894899955
1202	18	1.1745208067009756
1202	19	1.326138359693573
1202	20	1.2925134748353386
1202	21	1.2900330958517117
1202	22	1.1679572304640047
1202	23	1.1923385835410443
1202	24	1.020224684758709
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2211	7	-0.1666890667629488
2211	8	-0.1578288963689829
2211	9	-0.42858160227002173
2211	10	-0.5554062609324859
2211	11	-0.2012574571640542
2211	12	-0.25994703286588816
2211	13	-0.22045850526283317
2211	14	-1.4109065751576892
2211	15	-0.6468887446204903
2211	16	-0.36607723800823777
2211	17	-0.2656384821807265
2211	18	-0.4465478957083562
2211	19	-0.39464124455205507
2211	20	-0.4572617925472642
2211	21	-0.4512905222418766
2211	22	-0.34828827410251506
2211	23	-0.36325926614978243
2211	24	-0.6322325324625169
2211	25	-0.6336516122148481
2212	1	-0.29122066820275805
2212	2	-0.24054736210679195
2212	3	-0.17556838607334768
2212	4	0.08813588601143607
2212	5	-0.13638543389964042
2212	6	-0.17052942407575955
2212	7	-0.27960755055311637
2212	8	-0.5019348716065224
2212	9	-0.5376048475683035
2212	10	-0.5515810796167742
2212	11	-0.24790408341274173
2212	12	-0.22406466942532433
2212	13	-0.39481356276623814
2212	14	-0.25119941712893024
2212	15	-0.2534305379652082
2212	16	-0.22134982140750736
2212	17	-0.40545609553650763
2212	18	-0.16559377944022202
2212	19	-0.1087106889138667
2212	20	-0.47237049272634835
2212	21	-0.343510159669961
2212	22	-0.3355942410105399
2212	23	-0.7735731401480095
2212	24	-0.5134404014749308
2212	25	-0.6482590540421
2213	1	-0.32863098151213777
2213	2	-0.2995171931374401
2213	3	-0.22250779764952355
2213	4	-0.2604787312295045
2213	5	-0.16717149680744114
2213	6	0.06397465877289221
2213	7	-0.642485478989677
2213	8	-0.2289660992910285
2213	9	-0.42678562590191405
2213	10	-0.20241070310807174
2213	11	-0.13745544146717492
2213	12	-0.4116392689102071
2213	13	-0.32874616300036763
2213	14	-0.2192502825234044
2213	15	-0.33421294581999916
2213	16	-0.694663742511608
2213	17	-0.5168329885708332
2213	18	-0.5556938367002644
2213	19	-0.8488639981635586
2213	20	-0.4034684044629273
2213	21	-1.163799366291066
2213	22	-0.522483279043243
2213	23	-0.6336602113062284
2213	24	-0.7271969472924553
2213	25	-0.6892341104012516
2214	1	-1.060635024966679
2214	2	-0.964890262731771
2214	3	-0.8103585495075016
2214	4	-1.3696375771183256
2214	5	-1.2007438788190896
2214	6	-2.650908821694035
2214	7	-1.2384279488418102
2214	8	-1.810627095640914
2214	9	-1.2525215285982583
2214	10	-1.6590014645487585
2214	11	-1.3858685962357171
2214	12	-1.3151097684163773
2214	13	-1.570809341557755
2214	14	-1.823926345496936
2214	15	-2.221952504968044
2214	16	-1.6150051996223453
2214	17	-1.573244721123011
2214	18	-1.6253247314042696
2214	19	-3.4288759196830476
2214	20	-1.9300863759436382
2214	21	-2.2636566332312
2214	22	-2.0432412489164022
2214	23	-2.063723111099435
2214	24	-1.4538754652532404
2214	25	-1.264182706577074
2215	1	-1.5699593907448417
2215	2	-1.43859328776243
2215	3	-1.414678732844365
2215	4	-1.2933919254838457
2215	5	-2.083962460367644
2215	6	-1.849147561980466
2215	7	-1.9293600386092358
2215	8	-2.7865725721361443
2215	9	-2.232777351905419
2215	10	-1.775429397271843
2215	11	-2.3588361837351357
2215	12	-3.0591860243495574
2215	13	-2.4461471939635366
2215	14	-2.386385056261517
2215	15	-3.6157907737764816
2215	16	-3.0323104859181598
2215	17	-2.860510227038816
2215	18	-3.463910503062774
2215	19	-4.045990414224544
2215	20	-3.3826104880898455
2215	21	-2.5944975609704883
2215	22	-3.585248351163628
2215	23	-3.5596374237649684
2215	24	-2.5068406250798887
2215	25	-2.719311733087963
2216	1	-1.380859901196601
2216	2	-1.1987162458338432
2216	3	-1.5897081287191774
2216	4	-1.128683535066557
2216	5	-1.1524837620454136
2216	6	-1.6254011610506431
2216	7	-2.6686678001956885
2216	8	-1.9194687402046746
2216	9	-1.971898226298812
2216	10	-1.9765328346554938
2216	11	-2.416925246348164
2216	12	-1.848164453427522
2216	13	-2.2354378137881383
2216	14	-2.27456191091985
2216	15	-2.356651459650273
2216	16	-2.072369906763811
2216	17	-2.374709307497838
2216	18	-2.1594005475075946
2216	19	-2.4422676537511663
2216	20	-2.6826830330278497
2216	21	-2.567010410662064
2216	22	-2.9115482337274585
2216	23	-2.1034947724832094
2216	24	-2.3651161918298556
2216	25	-2.0963763503846877
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71515.0
3	0.0
4	1246.0
5	10403.0
6	12016.0
7	9247.0
8	7338.0
9	6389.0
10	5555.0
11	4916.0
12	4421.0
13	4167.0
14	3960.0
15	4043.0
16	4113.0
17	4272.0
18	4720.0
19	5420.0
20	6919.0
21	8920.0
22	11407.0
23	13783.0
24	16441.0
25	18775.0
26	21338.0
27	23169.0
28	26384.0
29	31585.0
30	40002.0
31	50672.0
32	64272.0
33	82687.0
34	108442.0
35	142398.0
36	195455.0
37	305972.0
38	995358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.284584137335635	19.617699057210565	13.481699703851987	27.616017101601813
2	13.64538730438451	20.798877183093992	39.50424385782292	26.051491654698573
3	16.397418933548707	25.023370508480404	30.61424913649408	27.964961421476808
4	11.406980047002325	17.532494040914965	37.953366372755326	33.107159539327384
5	12.658713782133951	38.12806293525922	36.1579399879632	13.055283294643624
6	31.332314945747857	37.92327181876771	18.00813362919026	12.736279606294168
7	27.0338412376725	31.913014667300953	22.970348683054198	18.082795411972345
8	24.826739504424687	36.77846815454466	20.756095820512357	17.63869652051829
9	26.00349160150357	14.979761261102512	20.147065017539667	38.86968211985425
10	14.283994016905083	29.172060426162194	35.059551405502624	21.484394151430102
11	33.77420736545759	22.490418443674283	24.905988832508683	18.829385358359442
12	22.52031227845545	25.083332975281536	32.02336502807842	20.37298971818459
13	28.44584141217857	22.008858123519655	25.93898987833291	23.606310585968867
14	20.81088837771627	21.875829736555154	27.97835144826354	29.33493043746504
15	22.803703304299855	30.490779332540797	23.644117423394544	23.061399939764808
16	22.23021913498421	28.132800123061745	27.297621651304716	22.339359090649328
17	21.646423740088387	28.395775284607065	27.817236109989054	22.140564865315497
18	21.526476123866427	27.761340481444996	29.117571371924488	21.594612022764093
19	23.106801538901074	27.129210550623274	27.725352127803543	22.03863578267211
20	23.242793177137173	27.384766878645944	27.935552112789818	21.436887831427068
21	22.844982577364366	26.76633219044051	27.469681692678538	22.919003539516584
22	22.868263511641246	27.709792650732584	27.42101159291408	22.000932244712086
23	22.206837592150258	27.432723867131788	27.725070025604452	22.635368515113502
24	22.59773512278109	27.057721719107107	28.182771123674673	22.16177203443713
25	22.592182543927596	27.169614506041967	27.845536052228077	22.392666897802364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	243.0
1	243.0
2	447.5
3	652.0
4	652.0
5	652.0
6	1546.5
7	2441.0
8	2441.0
9	2441.0
10	3614.0
11	4787.0
12	4787.0
13	4787.0
14	8247.5
15	11708.0
16	11708.0
17	11708.0
18	20922.5
19	30137.0
20	30137.0
21	30137.0
22	46323.0
23	62509.0
24	62509.0
25	62509.0
26	87831.0
27	113153.0
28	113153.0
29	113153.0
30	142817.0
31	172481.0
32	172481.0
33	172481.0
34	196923.0
35	221365.0
36	221365.0
37	221365.0
38	239487.5
39	257610.0
40	257610.0
41	257610.0
42	272094.0
43	286578.0
44	286578.0
45	286578.0
46	293613.5
47	300649.0
48	300649.0
49	300649.0
50	290727.5
51	280806.0
52	280806.0
53	280806.0
54	252605.5
55	224405.0
56	224405.0
57	224405.0
58	192922.0
59	161439.0
60	161439.0
61	161439.0
62	131449.0
63	101459.0
64	101459.0
65	101459.0
66	78174.0
67	54889.0
68	54889.0
69	54889.0
70	40087.5
71	25286.0
72	25286.0
73	25286.0
74	17670.5
75	10055.0
76	10055.0
77	10055.0
78	6782.5
79	3510.0
80	3510.0
81	3510.0
82	2328.5
83	1147.0
84	1147.0
85	1147.0
86	736.5
87	326.0
88	326.0
89	326.0
90	202.0
91	78.0
92	78.0
93	78.0
94	41.5
95	5.0
96	5.0
97	5.0
98	3.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0797346759919578
2	1.2888148059044902E-4
3	0.0
4	0.02581925661161995
5	0.0652999501658275
6	0.07823105871840257
7	0.03037306892581582
8	0.06246455759283762
9	0.042702730568968776
10	0.10748715481243448
11	0.003050195040640627
12	0.013317752994346397
13	0.005971508600690805
14	0.007475125874246043
15	0.007604007354836492
16	0.01860189369855481
17	0.028010241781657588
18	8.162493770728438E-4
19	0.0036516419500627222
20	0.005756706133040056
21	4.296049353014968E-5
22	2.148024676507484E-4
23	0.0
24	0.0
25	0.003565720963002423
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2327720.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.62462899802524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.84359317780026	55.59080552873602
2	13.32906274256025	18.560620966843164
3	3.680984131856625	7.688614635844067
4	1.3842646652344848	3.8551565500810647
5	0.6521565776787597	2.270307988475274
6	0.3492274881705582	1.4588900579872417
7	0.17637554388626975	0.8596057265184516
8	0.13078757375778538	0.7284829040350135
9	0.083624468514388	0.5240090335924177
>10	0.3374066699561877	3.916809363669507
>50	0.018541845195817516	0.8783119048138928
>100	0.011690040039524672	1.679580298134856
>500	0.0014204522439961946	0.6706175608345502
>1k	8.646231050365315E-4	1.318187480434628
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4973	0.21364253432543434	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3675	0.15787981372330007	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3175	0.1363995669582252	No Hit
GTCCTACAGTGGACATTTCTAAATT	2393	0.10280446101764817	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2371	0.10185933015998487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.296049353014967E-5	0.0
5	0.0	0.0	0.0	4.296049353014967E-5	0.0
6	0.0	0.0	0.0	4.296049353014967E-5	0.0
7	0.0	0.0	0.0	4.296049353014967E-5	0.0
8	0.0	0.0	0.0	4.296049353014967E-5	0.0
9	0.0	0.0	0.0	1.718419741205987E-4	0.0
10	0.0	0.0	0.0	2.1480246765074836E-4	0.0
11	0.0	0.0	0.0	2.1480246765074836E-4	0.0
12	0.0	0.0	0.0	2.5776296118089803E-4	0.0
13	0.0	0.0	0.0	2.5776296118089803E-4	4.296049353014967E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTG	85	5.296905E-5	11.18233	7
AACTGCG	70	0.0014853748	10.862835	7
TAGGACC	495	0.0	10.763763	4
GTAAGAC	205	0.0	10.66619	3
GTCCTAA	855	0.0	10.451494	1
GGCGAGG	730	0.0	10.400307	19
GGTATCA	1240	0.0	10.119729	1
TGTAGGA	1595	0.0	9.893821	2
GGACGTG	1605	0.0	9.7202015	6
GTAGGAC	1525	0.0	9.600331	3
TCCAACG	100	2.7852415E-4	9.490075	18
AGGACGT	1675	0.0	9.485996	5
TTAGGAC	1195	0.0	9.387489	3
TGGCGAG	1450	0.0	9.359178	18
TAAGACT	305	0.0	9.358423	4
TAATACA	285	0.0	9.347478	4
ATTATAC	245	1.8189894E-12	9.312804	3
GACGTGA	960	0.0	9.20795	7
TAGGACG	1815	0.0	9.173661	4
TCTATAC	260	0.0	9.141174	3
>>END_MODULE