FastQCFastQC Report
Thu 26 May 2016
SRR1512964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211339
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT38430.17378610877843698No Hit
GTCCTAAAGTGTGTATTTCTCATTT33850.15307467556986964No Hit
CTTTAGGACGTGAAATATGGCGAGG32140.1453418042190727No Hit
GTATCAACGCAGAGTACTTTTTTTT32000.14470870364064486No Hit
CTGTAGGACGTGGAATATGGCAAGA31970.14457303923098178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7300.012.8816754
GTCCTAA10150.011.2676191
GGCGAGG9450.011.15154419
TTAGGAC12550.011.1261123
TAGGACT4100.011.1203224
GGACCGC600.005907226411.0756946
GTCCTAC19650.010.8158151
TGGCGAG21550.010.75894418
AGGACCT14700.010.7255925
TGTAGGA21050.010.6951212
TCGTTGG803.762159E-410.69053112
CTAATAC2050.010.6572173
AGGACGT19100.010.6416975
GGACGTG19350.010.4992196
TAATACC1909.094947E-1210.4984624
GTAGGAC20300.010.4814613
GGACCTG14450.010.4460836
GATATAC4800.010.3247381
GACGTGA10700.010.2919667
CTGTAGG21700.010.1893211
Produced by FastQC (version 0.11.5)