##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512964_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2211339
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.164199609376944	31.0	30.0	34.0	23.0	34.0
2	29.207286625885946	31.0	30.0	34.0	23.0	34.0
3	29.350930363910734	31.0	30.0	34.0	23.0	34.0
4	32.78232600248085	35.0	33.0	37.0	26.0	37.0
5	32.87942961255601	35.0	35.0	37.0	26.0	37.0
6	32.9062129325264	37.0	35.0	37.0	26.0	37.0
7	32.72185178301473	36.0	33.0	37.0	25.0	37.0
8	32.73418684335599	37.0	33.0	37.0	25.0	37.0
9	34.06391738218338	38.0	35.0	39.0	25.0	39.0
10	33.91364734217594	38.0	34.0	39.0	24.0	39.0
11	34.01820932927968	38.0	35.0	39.0	25.0	39.0
12	33.92153351430966	38.0	34.0	39.0	24.0	39.0
13	33.94359119067678	38.0	35.0	39.0	24.0	39.0
14	34.95350238023207	39.0	35.0	41.0	24.0	41.0
15	34.90596918880371	39.0	35.0	41.0	23.0	41.0
16	34.809779956849674	39.0	34.0	41.0	22.0	41.0
17	34.763120896434245	39.0	34.0	41.0	22.0	41.0
18	34.45977482421284	39.0	34.0	41.0	17.0	41.0
19	34.701886956274	39.0	34.0	41.0	19.0	41.0
20	34.77929752064247	39.0	34.0	41.0	19.0	41.0
21	34.71220920899057	39.0	34.0	41.0	19.0	41.0
22	34.631479840947044	39.0	34.0	41.0	18.0	41.0
23	34.37311239932005	39.0	34.0	41.0	17.0	41.0
24	34.380326580411236	39.0	34.0	41.0	16.0	41.0
25	34.30284456612035	39.0	34.0	41.0	16.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6416926157730103
1101	2	0.8861590130040824
1101	3	0.8833618114446367
1101	4	0.8705524893704037
1101	5	0.6493023592618883
1101	6	0.7916512930219142
1101	7	0.8611696659598636
1101	8	0.7160572305206685
1101	9	0.6758713702446073
1101	10	0.7285882237614132
1101	11	0.9912049942306638
1101	12	1.086536261706378
1101	13	1.0182664813278066
1101	14	0.9804341350326595
1101	15	0.6974828846900607
1101	16	1.1823807430615574
1101	17	1.1562112001842024
1101	18	1.3528987593183714
1101	19	0.9433725305168537
1101	20	1.058692406993302
1101	21	1.1373309073020934
1101	22	1.1678927540228727
1101	23	1.368673389941577
1101	24	1.2327807271892652
1101	25	1.2477803230598354
1102	1	0.6856983166449098
1102	2	0.6930252101871837
1102	3	0.7084498396136532
1102	4	0.8054870970162824
1102	5	0.7372466919245326
1102	6	0.6971593413116537
1102	7	0.846313864484344
1102	8	0.829546499467682
1102	9	0.9471406526054409
1102	10	1.005830846162489
1102	11	1.004929004961717
1102	12	1.0942198633160345
1102	13	1.017721545043429
1102	14	1.109097783590677
1102	15	1.2503626633620897
1102	16	1.2875408704928049
1102	17	1.1734898722164004
1102	18	1.3139022804451272
1102	19	1.3884303775993487
1102	20	1.3304940501857914
1102	21	1.2291526712189338
1102	22	1.4522656581140865
1102	23	1.6249778835700184
1102	24	1.5326465890269532
1102	25	1.382995782483043
1103	1	0.6060058132904764
1103	2	0.8462919761835117
1103	3	0.8213111101003925
1103	4	0.9865661683662594
1103	5	0.5050414842514499
1103	6	0.8405519637519276
1103	7	0.6455978060060588
1103	8	0.6782371726319383
1103	9	1.0216128608794364
1103	10	1.014898965182475
1103	11	0.9715395243021874
1103	12	1.1269336553219205
1103	13	1.1605130789732883
1103	14	1.2947247197645098
1103	15	1.1291340007179969
1103	16	1.3061382574641627
1103	17	1.2915177599793637
1103	18	1.5518333492268042
1103	19	1.409747840138074
1103	20	1.332135081842317
1103	21	1.2153406690677002
1103	22	1.342089478299961
1103	23	1.7135382002724882
1103	24	1.4491652558692323
1103	25	1.334623334665416
1104	1	0.6866400133366888
1104	2	0.6076706409110599
1104	3	0.6288559974911507
1104	4	0.824649942305065
1104	5	0.476295714743614
1104	6	0.5515211712057493
1104	7	0.6771441953064823
1104	8	0.6633302094796889
1104	9	0.7580834411194743
1104	10	0.821632243694971
1104	11	0.8445826243524763
1104	12	0.7630797279300765
1104	13	0.8056418966101973
1104	14	1.0204950431168243
1104	15	1.0718115114896136
1104	16	0.911928085254246
1104	17	1.0144891625319303
1104	18	1.3221332554424023
1104	19	1.0996502160096568
1104	20	1.1045741899390364
1104	21	1.1956296337694283
1104	22	1.110597626669609
1104	23	1.430827321281555
1104	24	1.2172491657274662
1104	25	0.9138725157508674
1105	1	0.6358468959822048
1105	2	0.72842779913125
1105	3	0.7370829097105336
1105	4	0.8583759135377633
1105	5	0.5213605756120145
1105	6	0.4448855346954659
1105	7	0.7630310752634699
1105	8	0.8301537072616512
1105	9	0.6674933080370664
1105	10	0.8956660520025395
1105	11	0.7910673669254038
1105	12	0.6880931705970994
1105	13	0.8397879511874251
1105	14	0.9568104080852322
1105	15	1.1573421607704333
1105	16	1.11787310474611
1105	17	1.0620190724660645
1105	18	1.0939271793569034
1105	19	1.1375904633547336
1105	20	1.0992391314634347
1105	21	1.1053755673626497
1105	22	0.7005895610693926
1105	23	1.1085612236877935
1105	24	1.2418744513841418
1105	25	1.2649613717799042
1106	1	0.465011447555014
1106	2	0.5357694945904861
1106	3	0.5682591202265073
1106	4	0.5996112375155533
1106	5	0.5454628206135652
1106	6	0.5517146951533078
1106	7	0.24506451285046182
1106	8	0.5301692634358162
1106	9	0.7184266784868854
1106	10	0.7548351885529954
1106	11	0.6312022962676238
1106	12	0.6481956775973785
1106	13	0.5963371005103184
1106	14	0.7391721628216814
1106	15	0.8517583129916915
1106	16	0.8354589502651137
1106	17	0.7997698134312046
1106	18	1.1458815260794637
1106	19	0.7830013919764554
1106	20	0.8259433512803653
1106	21	0.7454155221678747
1106	22	0.8661711163593111
1106	23	1.0154779899685593
1106	24	0.7211912897145538
1106	25	0.9280236456013213
1107	1	0.5658570610252411
1107	2	0.6148874551867252
1107	3	0.7858409604409609
1107	4	0.8202127139352129
1107	5	0.6874021411593816
1107	6	0.5912574968265929
1107	7	0.7823230849186231
1107	8	0.6054323062507052
1107	9	0.8520209522148008
1107	10	0.6638746347634736
1107	11	0.7514982209360994
1107	12	0.895324732565463
1107	13	0.8955358581200841
1107	14	1.0623831232793606
1107	15	1.2031487536669943
1107	16	1.133023186213542
1107	17	1.1297279011818233
1107	18	1.5461575238080485
1107	19	1.329993809647675
1107	20	1.1818493903125002
1107	21	1.2420945790982074
1107	22	1.2830756168202413
1107	23	1.3788631189290186
1107	24	1.346677189081909
1107	25	1.3126489062013178
1108	1	0.4719547630767522
1108	2	0.545970840961072
1108	3	0.4172504645289621
1108	4	0.6520839389621287
1108	5	0.5067837643876061
1108	6	0.730041623687292
1108	7	0.6987040260536475
1108	8	0.7251252635063565
1108	9	0.925803660648036
1108	10	0.7905733276501721
1108	11	0.7527136044659244
1108	12	0.7511297009496474
1108	13	0.7041091120982799
1108	14	0.979482158316678
1108	15	0.9310077250166771
1108	16	0.9561210413880161
1108	17	1.1055846337681672
1108	18	1.1590638696749096
1108	19	1.1251409636965803
1108	20	1.1003130917697561
1108	21	1.094635964299428
1108	22	0.9459821306903464
1108	23	1.1471851936443827
1108	24	1.200994608667152
1108	25	1.1968682646880993
1109	1	0.20937307412349782
1109	2	0.0894371109867933
1109	3	0.38871123507576044
1109	4	0.4106838877510839
1109	5	0.40493739425571107
1109	6	0.2649393402292688
1109	7	0.4458049754150579
1109	8	0.41414114708440763
1109	9	0.659045172906886
1109	10	0.7221323777342477
1109	11	0.24811455920939096
1109	12	0.4229701508239856
1109	13	0.6349554479807438
1109	14	0.4446958339485363
1109	15	0.7226484192035727
1109	16	0.8094366302579346
1109	17	0.48701948891756786
1109	18	-1.0082383684434006
1109	19	0.359422516793785
1109	20	0.5788713214985819
1109	21	0.6839816209016547
1109	22	0.878625908956316
1109	23	0.4224523799237403
1109	24	0.8510547082514321
1109	25	0.9319276058922767
1110	1	0.3508527815458038
1110	2	0.27732795498360474
1110	3	0.36400032111471603
1110	4	0.26840109980434335
1110	5	0.4695018554426156
1110	6	0.3649326192042679
1110	7	0.39820241421274716
1110	8	0.3326690458952797
1110	9	0.7260928069421553
1110	10	0.8058747100282773
1110	11	0.7754078221194973
1110	12	0.21440985995113948
1110	13	0.41330751822037826
1110	14	0.5940088140510298
1110	15	0.6868732227365371
1110	16	0.7973561615565998
1110	17	0.48403502596024595
1110	18	0.9281476216619424
1110	19	0.7513107313944758
1110	20	0.7739867755939827
1110	21	0.6605157358410167
1110	22	0.737564052576424
1110	23	0.7183828812466331
1110	24	0.9448297276768329
1110	25	0.9260785109356675
1111	1	-0.07416810904789273
1111	2	0.19659411624892087
1111	3	0.09281977604640801
1111	4	-0.08869213768395667
1111	5	-0.0010821750828213794
1111	6	0.2354144620063252
1111	7	0.16935023907406332
1111	8	0.07025550274991588
1111	9	0.39321755650672685
1111	10	0.30219494521105617
1111	11	0.12272627530946778
1111	12	0.2715262532782319
1111	13	0.2882780700340817
1111	14	0.2208997903213259
1111	15	0.39177017925390345
1111	16	0.3567881387631999
1111	17	0.3466031091601849
1111	18	0.5684407947166008
1111	19	0.13425695950969185
1111	20	0.17202205301099838
1111	21	0.43565844628228945
1111	22	0.24970375275864853
1111	23	0.4296773933128364
1111	24	0.5250637023262215
1111	25	-0.008669845503163742
1112	1	-0.263712146131752
1112	2	0.057230441575512714
1112	3	-0.004745331412504328
1112	4	0.14038582270374178
1112	5	0.07623093069045694
1112	6	0.1938417692123906
1112	7	-0.03813985784966434
1112	8	0.1377953257587592
1112	9	0.42770470357794466
1112	10	0.46319536661855665
1112	11	0.32187166089732955
1112	12	0.43411959503971076
1112	13	0.12605219561351788
1112	14	0.34448175408027737
1112	15	0.3334709799281512
1112	16	0.4109878859186935
1112	17	0.5063064382794451
1112	18	0.38276780693741586
1112	19	0.18159872099304408
1112	20	0.2704662165171072
1112	21	0.26196185968304775
1112	22	0.3080594206895384
1112	23	0.5910781518463395
1112	24	0.32170929861783293
1112	25	0.5882446087741258
1113	1	-0.24587594029900117
1113	2	-0.1595903391410367
1113	3	-0.320246380320409
1113	4	-0.05393008367608587
1113	5	-0.2543909277275418
1113	6	-0.06431034300018723
1113	7	-0.17109127901703403
1113	8	0.14134499463293082
1113	9	-0.016500224232515848
1113	10	-0.4088575312466034
1113	11	-0.3648382235171326
1113	12	0.07917582472997253
1113	13	4.273344395642198E-4
1113	14	0.031170084465699688
1113	15	-0.34381057985959984
1113	16	-0.28957202523263703
1113	17	-0.3600146509394335
1113	18	-0.3665302885749
1113	19	-0.18107225099494428
1113	20	-0.3353359306590633
1113	21	-0.32961092629860644
1113	22	-0.18313575844747731
1113	23	-0.6559757743599022
1113	24	-0.035116006799462696
1113	25	-0.027671889930168447
1114	1	-1.5588618314593816
1114	2	-1.2808111856714142
1114	3	-1.145757465837253
1114	4	-1.4014682351301637
1114	5	-1.4340626812547512
1114	6	-1.7789662600747604
1114	7	-2.088741725317064
1114	8	-1.5986492466570184
1114	9	-1.6606119432074635
1114	10	-1.8724336901870018
1114	11	-2.490953973589118
1114	12	-1.9775474692521726
1114	13	-1.9952536330237258
1114	14	-2.2351702552981934
1114	15	-2.166442310351968
1114	16	-2.885886450801305
1114	17	-4.698626801817632
1114	18	-3.747181022810434
1114	19	-2.753775965362923
1114	20	-2.04740042821161
1114	21	-2.9731467742686597
1114	22	-3.1621164667688113
1114	23	-3.1494972255548355
1114	24	-2.6466910139978523
1114	25	-2.394501720048222
1115	1	-1.1579701178817317
1115	2	-0.822122514526999
1115	3	-0.8404873596625251
1115	4	-1.027287525854156
1115	5	-0.7925218393340714
1115	6	-0.9340401558489297
1115	7	-1.8520809895421237
1115	8	-1.262616752393992
1115	9	-0.9485566514081043
1115	10	-0.7331299315291204
1115	11	-1.6452916648237235
1115	12	-1.2861488647420671
1115	13	-1.0815548415174945
1115	14	-1.2477165035531428
1115	15	-2.37872186452757
1115	16	-1.2719873318771278
1115	17	-1.326779580497515
1115	18	-1.3248950535886763
1115	19	-1.4229214515519928
1115	20	-1.2127366930659065
1115	21	-2.011988745070397
1115	22	-2.0287314107407255
1115	23	-1.3087445665838402
1115	24	-1.295311762026671
1115	25	-1.563297131900832
1116	1	-1.1711500614726837
1116	2	-1.9036246069564449
1116	3	-1.6069803791389532
1116	4	-1.2776473642953334
1116	5	-1.6691382746742356
1116	6	-1.5795564345010078
1116	7	-1.09023611625717
1116	8	-1.5824211952449048
1116	9	-1.8469247216908968
1116	10	-2.884150156761361
1116	11	-1.3961337360175676
1116	12	-1.411709153600988
1116	13	-1.8767579860289132
1116	14	-1.6906739106665896
1116	15	-1.2752401240228721
1116	16	-1.1915965113586822
1116	17	-1.7222387306762172
1116	18	-1.4532889814954615
1116	19	-1.9694611686521242
1116	20	-2.0553940275523246
1116	21	-2.6055524985271106
1116	22	-1.7620438456522365
1116	23	-1.6923239988645236
1116	24	-2.1532361281009216
1116	25	-1.866123025846683
1201	1	0.16593902455029763
1201	2	0.22769701775467865
1201	3	0.4508053981429754
1201	4	0.45864914133285595
1201	5	0.5895594003205886
1201	6	0.5501118744041094
1201	7	0.4794580383159328
1201	8	-0.04746358160466002
1201	9	0.3774987314028522
1201	10	0.5842316747284997
1201	11	0.17052860015759563
1201	12	-0.3813880398432232
1201	13	0.06795684440890426
1201	14	0.6269842990412613
1201	15	0.2501459035793232
1201	16	0.7845375974897877
1201	17	0.8072348400439466
1201	18	1.088800382437924
1201	19	0.6327904413866676
1201	20	0.6172979097644813
1201	21	0.5904647439721558
1201	22	0.8438697362647005
1201	23	1.0456570456366379
1201	24	0.7631124204366344
1201	25	0.6698811883465723
1202	1	0.33996928451911046
1202	2	0.5057914729749733
1202	3	0.1694334999453666
1202	4	0.5201599961285694
1202	5	0.4645836027636534
1202	6	0.4037016153342492
1202	7	0.38455550501164026
1202	8	0.49824947423037713
1202	9	0.7426861966118352
1202	10	0.8483311536844766
1202	11	0.5075493485662861
1202	12	0.6324722671129095
1202	13	0.6241902700572695
1202	14	0.9080750167128429
1202	15	0.5628642009720295
1202	16	0.6521340701565848
1202	17	0.8910209319371702
1202	18	1.2301094580083287
1202	19	0.9202999062706496
1202	20	0.7006047068477415
1202	21	0.7637722152663287
1202	22	0.8813856839459362
1202	23	0.9132174739507306
1202	24	1.0981659424107235
1202	25	0.8839039974204113
1203	1	0.2824534250472155
1203	2	0.4882338528410095
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2211	15	0.050807404153012214
2211	16	-0.17933144181239413
2211	17	-0.11403896884892362
2211	18	-0.009821587441024349
2211	19	-0.195525011258475
2211	20	-0.3902396658358285
2211	21	-0.38972344892314936
2211	22	-0.47098607337014897
2211	23	0.007233469145674576
2211	24	-0.06339067240505614
2211	25	-0.07260326140843176
2212	1	0.1884474771378457
2212	2	0.0672012124925736
2212	3	0.1380453152809551
2212	4	0.06914634720339308
2212	5	0.18645526390200473
2212	6	0.03734406288348424
2212	7	0.11393475750372772
2212	8	0.09732473752346493
2212	9	0.15565509532884647
2212	10	0.3414873326803445
2212	11	0.061464402341734115
2212	12	0.35894750881391957
2212	13	0.2211929417670504
2212	14	0.15620748785205052
2212	15	0.20244447077376293
2212	16	0.4001351274239937
2212	17	0.32298341631703664
2212	18	-2.4887767877120375
2212	19	-0.702316433474536
2212	20	-0.1088285506651232
2212	21	-0.1650416721644703
2212	22	0.15896925708786824
2212	23	0.10136987433243405
2212	24	-0.3182996324908416
2212	25	0.04552394949591587
2213	1	-0.3794607306890647
2213	2	-0.22316752346920765
2213	3	-0.556963432667942
2213	4	-0.696845489350089
2213	5	-0.6184368300381848
2213	6	-0.4949505504725167
2213	7	-0.3933457329624872
2213	8	-0.8996682009500248
2213	9	-0.8308102713953929
2213	10	-0.758543043162355
2213	11	-0.7283759800315295
2213	12	-0.7312489123862349
2213	13	-0.578675957336344
2213	14	-0.6755510677790753
2213	15	-0.7731150543706917
2213	16	-1.4109620607457813
2213	17	-0.9078680099030478
2213	18	-0.7171388148727118
2213	19	-0.6194842362564827
2213	20	-1.415720047534748
2213	21	-1.0198746874573459
2213	22	-1.03722506277645
2213	23	-1.4766067583038165
2213	24	-1.1758503961388698
2213	25	-1.327336091972576
2214	1	-1.9598925972595715
2214	2	-3.8229951223342624
2214	3	-2.1265348050967177
2214	4	-1.677373575792501
2214	5	-1.7610923775802192
2214	6	-1.8335868022161783
2214	7	-1.9589368503308648
2214	8	-1.6401896366472535
2214	9	-1.9268667249934879
2214	10	-1.8660828288701623
2214	11	-1.604534354140263
2214	12	-2.1318503297723197
2214	13	-2.011376375815054
2214	14	-3.136721253438516
2214	15	-3.340032779470345
2214	16	-2.633944444908373
2214	17	-3.079528148186725
2214	18	-2.417217155468599
2214	19	-4.576611805322742
2214	20	-2.907338294761086
2214	21	-2.848549418512441
2214	22	-2.880948098107453
2214	23	-2.9724105699581713
2214	24	-3.1779899495024644
2214	25	-2.985875691977757
2215	1	-0.9713100297296364
2215	2	-2.676007653662584
2215	3	-1.4098141144812892
2215	4	-1.067794071476154
2215	5	-0.8481500216904934
2215	6	-1.3634069080362856
2215	7	-1.9185737202835256
2215	8	-1.8210194890560523
2215	9	-1.6326021747289374
2215	10	-3.1848152153920246
2215	11	-2.0562235771979083
2215	12	-2.401150246230131
2215	13	-1.678127698566378
2215	14	-3.0118357062371786
2215	15	-2.900349125891953
2215	16	-2.4812872463564375
2215	17	-2.708614196641193
2215	18	-1.4684808703112608
2215	19	-2.2366129192185937
2215	20	-2.4317983337474374
2215	21	-2.216101632380443
2215	22	-1.7144273517972337
2215	23	-2.3258742291060415
2215	24	-2.328131971223435
2215	25	-2.139829465299954
2216	1	-1.9358399189800224
2216	2	-1.9576261097406658
2216	3	-2.357648614464857
2216	4	-3.1684559400883323
2216	5	-2.1673384274875964
2216	6	-2.369972486924844
2216	7	-2.0291253650960392
2216	8	-2.5836433018662
2216	9	-2.949546405561364
2216	10	-2.2923024151028244
2216	11	-2.9159526514362994
2216	12	-3.2470688107573906
2216	13	-3.736854045143655
2216	14	-3.268153560915284
2216	15	-2.8893719659458483
2216	16	-2.195268251318808
2216	17	-2.311786433651939
2216	18	-2.3365477801669883
2216	19	-2.6695323053305273
2216	20	-2.485042804482596
2216	21	-3.0176435824938217
2216	22	-3.58707988720753
2216	23	-3.4838961161217163
2216	24	-3.151340095052678
2216	25	-3.427302862388256
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	104684.0
3	2.0
4	823.0
5	4957.0
6	6188.0
7	5606.0
8	4922.0
9	4593.0
10	4355.0
11	4154.0
12	3865.0
13	3724.0
14	3929.0
15	4029.0
16	4097.0
17	4439.0
18	5081.0
19	5981.0
20	7711.0
21	9924.0
22	12362.0
23	15020.0
24	17413.0
25	19962.0
26	22258.0
27	24735.0
28	28404.0
29	33727.0
30	41960.0
31	52118.0
32	66036.0
33	84420.0
34	109865.0
35	144668.0
36	199210.0
37	310003.0
38	836114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08424218880474	18.773008807789775	12.652529788918015	25.49021921448747
2	14.629029777146425	20.213805521122683	41.62661394667077	23.530550755060126
3	18.382400175162246	25.027436958329304	29.885248823581033	26.70491404292742
4	12.221514497893716	17.127446474326497	38.61769676746913	32.03334226031066
5	13.017342975608543	38.1207063863862	36.012404966510665	12.849545671494592
6	31.486462588203157	37.44006527519655	18.342366415839003	12.731105720761285
7	27.534629847568066	31.787092811519376	22.753526305839017	17.924751035073548
8	24.76277968205376	37.12044522967559	20.926183749514884	17.19059133875577
9	26.51479446775829	15.12632446422126	20.353440734833963	38.005440333186485
10	14.980055570305753	28.87977844246646	34.789245793441836	21.35092019378595
11	33.85066015594435	22.395458535983035	25.19248800384637	18.561393304226257
12	23.162891971485006	24.775261917394083	32.010120733255626	20.051725377865285
13	28.745855778842195	22.18077624145714	25.872197420971453	23.20117055872921
14	20.839249535335956	22.219422753588507	27.865322740787345	29.07600497028819
15	22.88317833104147	31.011344720272128	23.41142252297894	22.69405442570746
16	22.133710498213155	28.09006907201247	27.84500839258196	21.931212037192413
17	21.68970611250173	28.594393277505535	27.942742280556853	21.773158329435883
18	21.554706014164555	27.54610044657509	29.72804867283678	21.171144866423575
19	23.42040426035246	27.058855990334735	27.522352737896593	21.998387011416217
20	23.480450070832422	27.464163137107324	27.98093223788838	21.074454554171876
21	22.937650145854356	26.91663119632236	27.246064374943174	22.899654282880107
22	23.156225505028917	28.125032509194163	27.139873889203237	21.57886809657368
23	22.291140282553492	27.87142251037842	27.64788736216765	22.18954984490044
24	23.03635557726578	27.00602458682732	28.136313061412793	21.821306774494108
25	22.651904213523043	27.386995133963023	27.854827635182016	22.10627301733192
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	310.0
1	310.0
2	454.5
3	599.0
4	599.0
5	599.0
6	1187.0
7	1775.0
8	1775.0
9	1775.0
10	2685.0
11	3595.0
12	3595.0
13	3595.0
14	6651.5
15	9708.0
16	9708.0
17	9708.0
18	18110.5
19	26513.0
20	26513.0
21	26513.0
22	42185.5
23	57858.0
24	57858.0
25	57858.0
26	83043.0
27	108228.0
28	108228.0
29	108228.0
30	139211.0
31	170194.0
32	170194.0
33	170194.0
34	193047.0
35	215900.0
36	215900.0
37	215900.0
38	231633.0
39	247366.0
40	247366.0
41	247366.0
42	260369.5
43	273373.0
44	273373.0
45	273373.0
46	280125.5
47	286878.0
48	286878.0
49	286878.0
50	276090.0
51	265302.0
52	265302.0
53	265302.0
54	237029.5
55	208757.0
56	208757.0
57	208757.0
58	179365.5
59	149974.0
60	149974.0
61	149974.0
62	122595.5
63	95217.0
64	95217.0
65	95217.0
66	73351.5
67	51486.0
68	51486.0
69	51486.0
70	37651.0
71	23816.0
72	23816.0
73	23816.0
74	16632.0
75	9448.0
76	9448.0
77	9448.0
78	6455.0
79	3462.0
80	3462.0
81	3462.0
82	2273.5
83	1085.0
84	1085.0
85	1085.0
86	725.0
87	365.0
88	365.0
89	365.0
90	232.5
91	100.0
92	100.0
93	100.0
94	56.5
95	13.0
96	13.0
97	13.0
98	15.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35431021657014145
2	0.0415585308267977
3	0.03694594089825214
4	0.03667461207892593
5	0.04223685287511322
6	0.017726816195978996
7	0.017410265906765084
8	0.0237412716910433
9	0.02012355410002718
10	0.02016877556991488
11	0.019083460292610044
12	0.01953567499148706
13	0.019400010581823952
14	0.02722332487239632
15	0.027992089860487245
16	0.020711433208567297
17	0.02229418465463685
18	0.10419026662126432
19	0.024238707859808015
20	0.02202285583531064
21	0.02586668077576527
22	0.01926434617216085
23	0.02265595641373846
24	0.01804336648519291
25	0.02283684229328927
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2211339.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.44493249959397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.23710279873097	56.41505120320449
2	12.427374047175041	17.260363037065535
3	3.405159236512224	7.094131599898811
4	1.2428990100054171	3.4525215141455354
5	0.5583399831412885	1.938694122053561
6	0.30688591368360657	1.2787002936500567
7	0.18377570239273952	0.8933603873410486
8	0.11994786260110728	0.6663816977443574
9	0.08765587758842971	0.5478530852089056
>10	0.3911338588107897	4.6679593138589475
>50	0.02212600033961526	1.0529565372368128
>100	0.013939096549221477	1.9154148800753952
>500	0.0026147231925933946	1.2344213104479953
>1k	0.001045889277036885	1.582191018068593
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3843	0.17378610877843698	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3385	0.15307467556986964	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3214	0.1453418042190727	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3200	0.14470870364064486	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3197	0.14457303923098178	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.522146988770152E-5	0.0
6	0.0	0.0	0.0	4.522146988770152E-5	0.0
7	0.0	0.0	0.0	4.522146988770152E-5	0.0
8	4.522146988770152E-5	0.0	0.0	4.522146988770152E-5	0.0
9	4.522146988770152E-5	0.0	0.0	4.522146988770152E-5	0.0
10	4.522146988770152E-5	0.0	0.0	4.522146988770152E-5	0.0
11	4.522146988770152E-5	0.0	0.0	4.522146988770152E-5	0.0
12	4.522146988770152E-5	0.0	0.0	4.522146988770152E-5	0.0
13	4.522146988770152E-5	0.0	0.0	4.522146988770152E-5	4.522146988770152E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	730	0.0	12.881675	4
GTCCTAA	1015	0.0	11.267619	1
GGCGAGG	945	0.0	11.151544	19
TTAGGAC	1255	0.0	11.126112	3
TAGGACT	410	0.0	11.120322	4
GGACCGC	60	0.0059072264	11.075694	6
GTCCTAC	1965	0.0	10.815815	1
TGGCGAG	2155	0.0	10.758944	18
AGGACCT	1470	0.0	10.725592	5
TGTAGGA	2105	0.0	10.695121	2
TCGTTGG	80	3.762159E-4	10.690531	12
CTAATAC	205	0.0	10.657217	3
AGGACGT	1910	0.0	10.641697	5
GGACGTG	1935	0.0	10.499219	6
TAATACC	190	9.094947E-12	10.498462	4
GTAGGAC	2030	0.0	10.481461	3
GGACCTG	1445	0.0	10.446083	6
GATATAC	480	0.0	10.324738	1
GACGTGA	1070	0.0	10.291966	7
CTGTAGG	2170	0.0	10.189321	1
>>END_MODULE