FastQCFastQC Report
Thu 26 May 2016
SRR1512964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211339
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39700.17952923545417504No Hit
GTCCTACAGTGGACATTTCTAAATT36500.16505836509011054No Hit
CTGTAGGACGTGGAATATGGCAAGA32870.1486429715208749No Hit
CTTTAGGACGTGAAATATGGCGAGG32790.14828119976177329No Hit
GTCCTAAAGTGTGTATTTCTCATTT31840.14398516012244164No Hit
TATCAACGCAGAGTACTTTTTTTTT29660.13412687968692272No Hit
GGTATCAACGCAGAGTACTTTTTTT25980.11748537876824856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAT400.00527507214.25202110
TAGGACC6800.011.8920274
GGCGAGG7950.011.69937619
AATCCCG1950.011.19430119
TGGCGAG16900.010.55783618
ACACCGT1303.784644E-710.2421896
CCTATAC2002.5465852E-119.9816113
TTATACT3050.09.9815064
TGTACCG1252.5241752E-69.8941675
GTAGGAC20350.09.7632243
GTATAGG1951.6189006E-109.7493851
TGTAGGA21350.09.6614812
AGGACGT19000.09.5636945
GGACGTG18750.09.5359666
GTCCTAG3400.09.505651
GATATAC4900.09.505651
AATGCCG1901.0713848E-99.49081918
GGTATCA14150.09.4720611
ATTTAGA7050.09.4382341
AGGACCT13550.09.4083055
Produced by FastQC (version 0.11.5)