##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512964_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2211339
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.43811102684844	31.0	31.0	34.0	27.0	34.0
2	30.570292931115493	31.0	31.0	34.0	27.0	34.0
3	30.65896499813009	31.0	31.0	34.0	27.0	34.0
4	34.14590707259267	37.0	35.0	37.0	30.0	37.0
5	33.80424530115012	37.0	35.0	37.0	29.0	37.0
6	33.674740960115116	37.0	35.0	37.0	29.0	37.0
7	33.54840121754285	37.0	35.0	37.0	28.0	37.0
8	33.43284363003592	37.0	35.0	37.0	28.0	37.0
9	34.88053889521236	39.0	35.0	39.0	27.0	39.0
10	34.76094438708855	39.0	35.0	39.0	27.0	39.0
11	34.803380214431165	39.0	35.0	39.0	27.0	39.0
12	34.652200318449594	39.0	35.0	39.0	27.0	39.0
13	34.640410629035166	39.0	35.0	39.0	27.0	39.0
14	35.689174748873874	40.0	36.0	41.0	27.0	41.0
15	35.71386431478846	40.0	36.0	41.0	27.0	41.0
16	35.69159409751286	40.0	36.0	41.0	27.0	41.0
17	35.66696015400624	40.0	36.0	41.0	27.0	41.0
18	35.58898206019068	40.0	36.0	41.0	27.0	41.0
19	35.56845422614986	40.0	36.0	41.0	26.0	41.0
20	35.54224838434993	40.0	36.0	41.0	26.0	41.0
21	35.48998819267421	40.0	36.0	41.0	26.0	41.0
22	35.45034207780897	40.0	36.0	41.0	26.0	41.0
23	35.34034989660111	40.0	36.0	41.0	25.0	41.0
24	35.331867253279576	40.0	36.0	41.0	25.0	41.0
25	35.23467546133813	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.660439837143663
1101	2	0.7080988697770216
1101	3	0.6781144026765666
1101	4	0.5738772463889745
1101	5	0.7817223122446748
1101	6	0.8227059222358974
1101	7	0.7025196601717525
1101	8	0.6905887250367329
1101	9	0.654472324805532
1101	10	0.9661514085188401
1101	11	1.005299308156772
1101	12	0.9460690601342279
1101	13	1.025571094435179
1101	14	1.0966927940105364
1101	15	1.0835296836285622
1101	16	1.2278881083277753
1101	17	1.1916772845723074
1101	18	0.6098603229737165
1101	19	1.0583124447457948
1101	20	0.9813241743930519
1101	21	1.1576868006451448
1101	22	1.0141407821545556
1101	23	1.0856856502378633
1101	24	0.9676683488429703
1101	25	1.014680659016868
1102	1	0.6025214937499683
1102	2	0.5225271058601884
1102	3	0.4305791914089596
1102	4	0.3517863677840154
1102	5	0.5242315342433344
1102	6	0.5609730248515916
1102	7	0.5289406863286956
1102	8	0.5203254788932057
1102	9	0.7681376500905799
1102	10	0.8280184440654494
1102	11	0.7067790197597219
1102	12	0.7071438421597165
1102	13	0.999586017305738
1102	14	0.893997455311677
1102	15	0.8390461155534439
1102	16	0.8713195301922951
1102	17	0.8154406983885423
1102	18	0.8579111278026943
1102	19	1.0148307210704104
1102	20	0.9464105258350344
1102	21	1.036027679250104
1102	22	0.9805014461384616
1102	23	0.9424135174410821
1102	24	0.9174210316062172
1102	25	0.9724858233361147
1103	1	0.6318995446385856
1103	2	0.5306163338052983
1103	3	0.5546656397698833
1103	4	0.6369804859314314
1103	5	0.6447119660255325
1103	6	0.7598825594015821
1103	7	0.7083852428162629
1103	8	0.6943636686787116
1103	9	0.7967564944640486
1103	10	0.834387924340831
1103	11	0.8829893599226537
1103	12	0.9138635829696611
1103	13	0.9289137859828713
1103	14	1.096749325974578
1103	15	0.8955280276768747
1103	16	0.8909292496820385
1103	17	1.1328709961305776
1103	18	1.0778745377186496
1103	19	1.082570223544046
1103	20	1.0808936617438079
1103	21	0.7193948313150358
1103	22	1.0085162865936184
1103	23	1.0233023670005466
1103	24	1.149232144729531
1103	25	1.197166534723081
1104	1	0.6245940453385685
1104	2	0.7111858022244206
1104	3	0.5962345577153236
1104	4	0.6340101367544264
1104	5	0.6867956788005998
1104	6	0.7805616808184013
1104	7	0.6347109669270168
1104	8	0.6785455356679648
1104	9	0.7728693347723095
1104	10	0.8809977540337925
1104	11	0.9573549559973031
1104	12	0.953793312625919
1104	13	0.9867352694074967
1104	14	1.149606692127925
1104	15	1.0272312340161562
1104	16	1.1570616963676414
1104	17	1.0429300531215944
1104	18	1.024374442575045
1104	19	1.1140364203825612
1104	20	1.157300088014317
1104	21	0.9374279412874458
1104	22	1.035437847492318
1104	23	0.9788680976354271
1104	24	0.9027251041032187
1104	25	0.9942349225827627
1105	1	0.4014407317211486
1105	2	0.452614421662517
1105	3	0.44152962425652476
1105	4	0.3921782373055791
1105	5	0.30327302790666266
1105	6	0.3573966048673327
1105	7	0.5137689720352299
1105	8	0.5610986342027147
1105	9	0.7237229441284114
1105	10	0.6816396413842369
1105	11	0.7524349398660988
1105	12	0.7313911080285322
1105	13	0.8325814852967284
1105	14	0.9019759621911021
1105	15	0.7839391248617034
1105	16	0.8232483477992858
1105	17	0.6382486443300834
1105	18	0.9933605294146801
1105	19	0.9852237439475573
1105	20	1.0327537780264109
1105	21	0.8606165419058129
1105	22	0.936634244857558
1105	23	0.858137824749285
1105	24	0.8136926400623494
1105	25	0.8968579229860438
1106	1	0.3574560586464308
1106	2	0.4212548120406119
1106	3	0.36854169257269476
1106	4	0.29610003068683
1106	5	0.4392184339228109
1106	6	0.38475991522119557
1106	7	0.4317175220360454
1106	8	0.46391430172633363
1106	9	0.4395239233486379
1106	10	0.4967184866248715
1106	11	0.4234726185574189
1106	12	0.5192884045292061
1106	13	0.4259209865166582
1106	14	0.5480173252394565
1106	15	0.6100759536946612
1106	16	0.7404968699627403
1106	17	0.4973522811998592
1106	18	0.700468882261454
1106	19	0.5798683263052169
1106	20	0.7361425677560618
1106	21	0.7115204374993169
1106	22	0.7041709656160435
1106	23	0.8144855625540615
1106	24	0.7696555672430776
1106	25	0.8344162586391519
1107	1	0.37907927128882335
1107	2	0.35115291073182675
1107	3	0.36535994975035635
1107	4	0.36052099209761224
1107	5	0.4669925675053861
1107	6	0.4448582203901097
1107	7	0.4119861673004763
1107	8	0.43130876623387593
1107	9	0.43012814693082646
1107	10	0.5417332676407725
1107	11	0.464037797191061
1107	12	0.48168944060193297
1107	13	0.4376810847417403
1107	14	0.5922158866233218
1107	15	0.5475210605016159
1107	16	0.5510617522552366
1107	17	0.550744946836538
1107	18	0.689996586554642
1107	19	0.6861581271239174
1107	20	0.7949699698613699
1107	21	0.7006391109161569
1107	22	0.842386319131009
1107	23	0.672499788531006
1107	24	0.6978894300659633
1107	25	0.7234168761500328
1108	1	0.37325867860214856
1108	2	0.27949712347578526
1108	3	0.24031728892444448
1108	4	0.29728960793801207
1108	5	0.31980491795249577
1108	6	0.27037196233614935
1108	7	0.23763719190431232
1108	8	0.37064471177777847
1108	9	0.27645773187158795
1108	10	0.5705671858654355
1108	11	0.5072674341168337
1108	12	0.6127902987145504
1108	13	0.5548878338643988
1108	14	0.6360325749144096
1108	15	0.621595614584983
1108	16	0.6039002152078794
1108	17	0.49263886190273354
1108	18	0.6101559208393539
1108	19	0.5378191752818111
1108	20	0.6964987034576495
1108	21	0.790793256300411
1108	22	0.7735973873772934
1108	23	0.7721290773540375
1108	24	0.8552587967368979
1108	25	0.7400909589602733
1109	1	0.2586961497551634
1109	2	0.33554157596612555
1109	3	0.29164706357954273
1109	4	0.3986765465124833
1109	5	0.27959609432235055
1109	6	0.20340699949677798
1109	7	0.4144836028093266
1109	8	0.5282431095571098
1109	9	0.5424457706680315
1109	10	0.4815255336732278
1109	11	0.547402539939398
1109	12	0.5689900152597929
1109	13	0.48225165570868
1109	14	0.6645336407238602
1109	15	0.619684822917705
1109	16	0.42521982920056445
1109	17	0.5889541131711837
1109	18	0.5135784168395077
1109	19	0.8202214951094575
1109	20	0.7893187194733358
1109	21	0.8051077214630382
1109	22	0.7844895196322597
1109	23	0.604202358073735
1109	24	0.22618951667610077
1109	25	0.4857661537334863
1110	1	0.24028808190231743
1110	2	0.2337977158683593
1110	3	0.23102964727269537
1110	4	0.24669335235354595
1110	5	0.26672860998561276
1110	6	0.27579079953152785
1110	7	0.40713834211552324
1110	8	0.4339517186983599
1110	9	0.5346506930811756
1110	10	0.5125220813613538
1110	11	0.4550545088718678
1110	12	0.6048899605080322
1110	13	0.20516133496825262
1110	14	0.49976345060081684
1110	15	0.5283106847662182
1110	16	0.5391498911979227
1110	17	0.5284388236589379
1110	18	0.27500842146226034
1110	19	0.47694055298567406
1110	20	0.3748953998115496
1110	21	0.46435732721754874
1110	22	0.4983304051027133
1110	23	0.5014614912910034
1110	24	0.38674136200728526
1110	25	0.5159320810874064
1111	1	-0.48550148186181374
1111	2	-0.22623530637131495
1111	3	-0.27243869466856907
1111	4	-0.1900766094896582
1111	5	0.08796704465091665
1111	6	-0.14985781564863743
1111	7	0.1304774140697731
1111	8	0.11332892731233102
1111	9	-0.35880727907141363
1111	10	-0.25205602932355475
1111	11	-0.014751787502731872
1111	12	-0.11972318596775011
1111	13	-0.022724838982433937
1111	14	0.12895943960683098
1111	15	0.26321257448401525
1111	16	-0.1342405263119204
1111	17	0.08614367732410244
1111	18	0.013687229842659576
1111	19	0.025018618365685086
1111	20	0.05488875509259117
1111	21	-0.05433648211928954
1111	22	-0.22189219298240204
1111	23	-0.08165790222737002
1111	24	-0.012133062872152323
1111	25	0.0884269717012316
1112	1	0.0707850752389021
1112	2	-0.1299487492705964
1112	3	0.05399535505751629
1112	4	-0.05890846789673532
1112	5	0.19709135986372672
1112	6	0.21757496985495095
1112	7	0.10946013636223029
1112	8	0.2456601536081635
1112	9	-0.010027675194464791
1112	10	0.2671871228045575
1112	11	0.057620736728196675
1112	12	0.21421191727708333
1112	13	0.10813061824470793
1112	14	0.22234755591529876
1112	15	0.12312492172380018
1112	16	-0.09321903452936908
1112	17	0.11961776076278596
1112	18	0.05296746583086076
1112	19	0.1571576828410315
1112	20	0.35307417439305055
1112	21	0.5301987054070523
1112	22	0.31784316310693583
1112	23	0.5293880311902441
1112	24	0.44039453931916483
1112	25	0.39715684949305796
1113	1	-0.2855217817394191
1113	2	-0.5581300663698521
1113	3	-0.24445374205901516
1113	4	-0.24101673164565796
1113	5	-0.1293519785427577
1113	6	-0.3141977256326882
1113	7	-0.12218734831548517
1113	8	0.00969890150005881
1113	9	-0.06200735517472111
1113	10	-0.4386352621240803
1113	11	-0.15315134175479272
1113	12	-0.19679528111047517
1113	13	-0.02115818106425138
1113	14	-0.16243587545321958
1113	15	-0.08805948039869804
1113	16	-0.30566229066856465
1113	17	-0.09684137296268602
1113	18	0.03436337714672533
1113	19	-0.13548605867515562
1113	20	0.16507869909380446
1113	21	-0.2072499392394107
1113	22	-0.33607662628880774
1113	23	-0.06175935065336802
1113	24	-0.3737898216696891
1113	25	-0.3910588553319201
1114	1	-0.7361408430696841
1114	2	-0.650557362655487
1114	3	-0.5234632439305962
1114	4	-0.4204005496704184
1114	5	-0.6251769137572722
1114	6	-0.9629164126467984
1114	7	-0.8043065536135003
1114	8	-1.1144013000547872
1114	9	-0.9056583773457518
1114	10	-1.1121164023486685
1114	11	-0.9219391332894915
1114	12	-0.839755258075634
1114	13	-0.9128944321440073
1114	14	-1.866150243434788
1114	15	-1.2357703931542403
1114	16	-1.1582393134154643
1114	17	-1.27190615845074
1114	18	-1.4323800723205906
1114	19	-1.4841759521390188
1114	20	-0.83117813937438
1114	21	-0.7865761701636771
1114	22	-0.9119476311837147
1114	23	-0.8265160451538023
1114	24	-1.5534615751968985
1114	25	-1.466785892475599
1115	1	-1.5907871145606407
1115	2	-1.5056476412607864
1115	3	-1.1384116482411386
1115	4	-2.540711901881341
1115	5	-1.5938544237783319
1115	6	-1.4052380217715452
1115	7	-1.280705303881362
1115	8	-1.2544516623437971
1115	9	-1.5920080524252853
1115	10	-1.5347467349303727
1115	11	-1.4208060585014408
1115	12	-1.8370041869265847
1115	13	-1.4675709803343366
1115	14	-2.207383476817512
1115	15	-2.204902820811938
1115	16	-1.6914634733350837
1115	17	-1.5950176109702738
1115	18	-1.495413824873694
1115	19	-1.1525577870058754
1115	20	-1.258868141432032
1115	21	-1.372761795311888
1115	22	-1.5990892567526558
1115	23	-1.9465750915366442
1115	24	-1.3202878593952079
1115	25	-1.1578474641329208
1116	1	-0.9400918831565228
1116	2	-0.9886351240742535
1116	3	-0.8261626859429256
1116	4	-1.0099707740709718
1116	5	-0.9090424352951629
1116	6	-1.563379317470357
1116	7	-0.9132189083032856
1116	8	-1.0851025029627834
1116	9	-1.1492617160338057
1116	10	-1.1495542683106734
1116	11	-1.0763000816696433
1116	12	-1.6480074230104833
1116	13	-1.45566413788724
1116	14	-1.7624013264400133
1116	15	-1.5358164774465237
1116	16	-1.8919709778214866
1116	17	-1.6847151563905385
1116	18	-2.6333217926974726
1116	19	-2.030945664051714
1116	20	-1.6818673053282396
1116	21	-1.6264022853698634
1116	22	-2.9681577229537197
1116	23	-2.8020701829947825
1116	24	-2.103767690331651
1116	25	-2.3103451844188996
1201	1	0.6447833785982517
1201	2	0.6937763949438569
1201	3	0.5540744185118776
1201	4	0.6674597031246421
1201	5	0.6374958389950436
1201	6	0.6977066291695024
1201	7	0.7859738791515056
1201	8	0.8307185180465737
1201	9	1.0641304517142558
1201	10	0.9869967597234535
1201	11	1.0111209063922644
1201	12	0.9779743875858742
1201	13	0.924840832021431
1201	14	1.078195876241054
1201	15	1.162399438181211
1201	16	1.1069367170824407
1201	17	1.1465855245904066
1201	18	1.1767821926717161
1201	19	0.9573531358440874
1201	20	1.2375105503686186
1201	21	1.158497653489846
1201	22	1.3467495085429704
1201	23	1.1870677054352328
1201	24	1.2786717704016226
1201	25	1.2299023533953317
1202	1	0.5245293048084605
1202	2	0.6254257568637343
1202	3	0.5899229907406109
1202	4	0.5652865830494065
1202	5	0.5451645559543863
1202	6	0.6720573986343226
1202	7	0.5985726337629345
1202	8	0.49414715714320323
1202	9	0.7764389498835129
1202	10	0.7753775989950284
1202	11	0.7794090503069029
1202	12	0.7074695591984792
1202	13	0.789964679380077
1202	14	0.7549052602197293
1202	15	0.9176817942544702
1202	16	0.7061879107815088
1202	17	0.8296984428172678
1202	18	0.7923735793679825
1202	19	0.9718014757293645
1202	20	1.0604973392921977
1202	21	1.0166686575800554
1202	22	0.9226183762489626
1202	23	0.983724483675168
1202	24	0.7723795673915248
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2211	10	-0.5867010255582414
2211	11	-0.31249806941494995
2211	12	-0.3485339534850098
2211	13	-0.35542777062808284
2211	14	-1.3798749882747927
2211	15	-0.6444972167867746
2211	16	-0.5132793069141783
2211	17	-0.3687792256179705
2211	18	-0.5469873298805226
2211	19	-0.5772213377327162
2211	20	-0.5736942985133524
2211	21	-0.6443808367546424
2211	22	-0.6395616470930037
2211	23	-0.5463998563785779
2211	24	-0.6868949419906016
2211	25	-0.7495229534626091
2212	1	-0.1007217651784238
2212	2	0.0052053935459497325
2212	3	0.04498354163649765
2212	4	0.11051864207768602
2212	5	0.08820117190447974
2212	6	0.10589640857536153
2212	7	0.02923661506965658
2212	8	-0.09734159201762793
2212	9	-0.181814871232298
2212	10	-0.13533567182054185
2212	11	-0.030522810545782875
2212	12	0.03237503179825296
2212	13	0.07912806069995071
2212	14	-0.007360965174292744
2212	15	0.008355628499259637
2212	16	0.10928476119182307
2212	17	0.0036914018134126536
2212	18	0.15292881848786521
2212	19	0.15834365288122143
2212	20	-0.017472409457880644
2212	21	-0.013589789701136112
2212	22	0.1317865723546916
2212	23	-0.5106376254492275
2212	24	-0.10834528124674137
2212	25	-0.2718780224267263
2213	1	-0.5509751610784868
2213	2	-0.6230702722048704
2213	3	-0.4954041690255231
2213	4	-0.5564122013832389
2213	5	-0.5119336668040333
2213	6	-0.30336061724606367
2213	7	-0.7866745973704354
2213	8	-0.5779074183947941
2213	9	-0.8194332713214862
2213	10	-0.6249861766128575
2213	11	-0.5051806089680326
2213	12	-0.8231091625620905
2213	13	-0.622392069865576
2213	14	-0.49091165471898535
2213	15	-0.70742139812522
2213	16	-0.997357542775859
2213	17	-0.865977217492734
2213	18	-1.0048751533535167
2213	19	-1.2517742938554477
2213	20	-0.9030828782039748
2213	21	-1.53109923502128
2213	22	-0.933748532962646
2213	23	-1.1437988072441527
2213	24	-1.2350146672123898
2213	25	-1.3111474091431958
2214	1	-1.042658533783655
2214	2	-0.9557231853608243
2214	3	-0.8030572765214288
2214	4	-1.4747129791749316
2214	5	-1.2983372115648493
2214	6	-2.8144400677390387
2214	7	-1.3914872320588216
2214	8	-1.8599187937602615
2214	9	-1.4514863218110037
2214	10	-1.67013618546612
2214	11	-1.4190709925951097
2214	12	-1.5078031203169004
2214	13	-1.655929532131232
2214	14	-1.7906599628816906
2214	15	-2.13245402564462
2214	16	-1.522722793927862
2214	17	-1.749359682846233
2214	18	-1.7221603537180101
2214	19	-3.3834889337003204
2214	20	-1.9843243218475521
2214	21	-2.462147159254606
2214	22	-2.1790891677201287
2214	23	-2.098220991366148
2214	24	-1.4849287689515123
2214	25	-1.2817604437400263
2215	1	-1.4644106919568642
2215	2	-1.3515148868367355
2215	3	-1.2179411528789892
2215	4	-1.1300936530819214
2215	5	-1.74891922214686
2215	6	-1.4918112735312974
2215	7	-1.7548467419446503
2215	8	-2.440361010413003
2215	9	-1.9182710614378493
2215	10	-1.3182520306345964
2215	11	-1.9194374643300023
2215	12	-2.9053436382784703
2215	13	-2.156313826199735
2215	14	-2.1355466239788825
2215	15	-3.1300814274825512
2215	16	-2.5341290874394176
2215	17	-2.444435149290122
2215	18	-3.0185880897246946
2215	19	-3.4985566028732507
2215	20	-3.2429152435963644
2215	21	-2.3350182258098826
2215	22	-3.273881704617928
2215	23	-3.274506148703935
2215	24	-2.3407059897813625
2215	25	-2.4789489706127625
2216	1	-1.0774834843588508
2216	2	-0.9047005978361113
2216	3	-1.2775976647591065
2216	4	-0.7128019905231753
2216	5	-0.7073286342208647
2216	6	-1.1919328680628283
2216	7	-2.361679171534842
2216	8	-1.516964513650045
2216	9	-1.5425736675044348
2216	10	-1.3920876479733195
2216	11	-1.9999598607281754
2216	12	-1.3831013036634658
2216	13	-1.7874446553429877
2216	14	-1.9229851858770672
2216	15	-2.0142853119348842
2216	16	-1.763040980699138
2216	17	-1.9050489059038824
2216	18	-1.7525232173989522
2216	19	-1.9760579338404227
2216	20	-2.308806334332175
2216	21	-2.1486815075476997
2216	22	-2.382755476348848
2216	23	-1.5290159912883183
2216	24	-1.763969849914794
2216	25	-1.6477269127074763
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57672.0
3	1.0
4	883.0
5	8240.0
6	9301.0
7	7345.0
8	6042.0
9	5167.0
10	4460.0
11	4150.0
12	3646.0
13	3661.0
14	3405.0
15	3409.0
16	3625.0
17	3788.0
18	4158.0
19	4908.0
20	6196.0
21	8228.0
22	10308.0
23	12872.0
24	15269.0
25	17630.0
26	19871.0
27	21977.0
28	24985.0
29	30093.0
30	38334.0
31	48896.0
32	62096.0
33	80229.0
34	104503.0
35	136349.0
36	187807.0
37	294194.0
38	957641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.20312811136656	19.64342607845622	13.658333484187468	27.49511232598976
2	13.504261445214869	20.81272930111575	40.146987865722984	25.536021387946402
3	16.805338304077303	25.04663464082169	30.30155032765216	27.846476727448845
4	11.472849352444571	17.616845849602132	37.89687421973099	33.013430578222305
5	12.682039686156182	38.23219175261744	36.08262677446695	13.003141786759425
6	31.487118494642925	37.75476307497912	18.057143322643626	12.700975107734333
7	27.266838352808236	31.801668579400467	22.808683344016597	18.1228097237747
8	24.70309766668205	37.329060003095044	20.69130754674464	17.27653478347827
9	26.182895083293296	14.950402238165166	20.231751796748256	38.63495088179329
10	14.57768405956398	29.15287828895249	34.85526843538167	21.414169216101868
11	33.80535127523079	22.384757105141535	25.08029264418985	18.729598975437824
12	22.908465635266364	24.874270952429196	32.04023872237579	20.17702468992865
13	28.70996714069805	22.040379044213083	25.936417748183604	23.313236066905265
14	20.715744839809208	22.103584151099376	27.79893515170674	29.381735857384676
15	22.7004610587392	31.070836621495424	23.41956797507253	22.80913434469285
16	21.99947896078004	28.104367050420493	27.76338487339923	22.132769115400237
17	21.563130168569668	28.652501133116026	27.95761377072969	21.826754927584613
18	21.478964478859112	27.611379752737154	29.621074366524052	21.288581401879682
19	23.24414866964652	27.06815743918035	27.59287330664607	22.094820584527056
20	23.35647555640374	27.454647905668004	28.013832874920748	21.175043663007507
21	22.74112900928262	26.815677574245804	27.351100261968213	23.092093154503363
22	23.070477272347002	28.0193837483839	27.16799836479017	21.742140614478934
23	22.04392904027831	27.78551818604022	27.751466419214783	22.419086354466682
24	22.979074207561215	27.01369035398478	28.111532474908856	21.895702963545148
25	22.533021478884706	27.308292723516793	27.898898741367024	22.25978705623148
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	133.0
1	133.0
2	286.5
3	440.0
4	440.0
5	440.0
6	1101.0
7	1762.0
8	1762.0
9	1762.0
10	2666.5
11	3571.0
12	3571.0
13	3571.0
14	6705.5
15	9840.0
16	9840.0
17	9840.0
18	18463.0
19	27086.0
20	27086.0
21	27086.0
22	42828.5
23	58571.0
24	58571.0
25	58571.0
26	83815.5
27	109060.0
28	109060.0
29	109060.0
30	139458.5
31	169857.0
32	169857.0
33	169857.0
34	192391.5
35	214926.0
36	214926.0
37	214926.0
38	232209.0
39	249492.0
40	249492.0
41	249492.0
42	261479.5
43	273467.0
44	273467.0
45	273467.0
46	280150.5
47	286834.0
48	286834.0
49	286834.0
50	276172.0
51	265510.0
52	265510.0
53	265510.0
54	236945.0
55	208380.0
56	208380.0
57	208380.0
58	178717.5
59	149055.0
60	149055.0
61	149055.0
62	121812.0
63	94569.0
64	94569.0
65	94569.0
66	72910.5
67	51252.0
68	51252.0
69	51252.0
70	37329.0
71	23406.0
72	23406.0
73	23406.0
74	16320.5
75	9235.0
76	9235.0
77	9235.0
78	6343.5
79	3452.0
80	3452.0
81	3452.0
82	2265.5
83	1079.0
84	1079.0
85	1079.0
86	687.5
87	296.0
88	296.0
89	296.0
90	177.5
91	59.0
92	59.0
93	59.0
94	32.0
95	5.0
96	5.0
97	5.0
98	3.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07683127733920489
2	0.0
3	0.0
4	0.02545968754677596
5	0.0655711313371672
6	0.07854969319493754
7	0.03237857243959429
8	0.061184648758060164
9	0.043276946682530355
10	0.10640611864576167
11	0.002396737904048181
12	0.010627045423609857
13	0.004205596699556242
14	0.005969234025176601
15	0.005607462266074989
16	0.01686760826811267
17	0.028082532800262648
18	3.6177175910161223E-4
19	0.0021254090847219716
20	0.00384382494045463
21	9.044293977540306E-5
22	9.044293977540306E-5
23	0.0
24	4.522146988770153E-5
25	0.0023062949642727775
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2211339.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.64461240239262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.53352813566813	55.281928242701206
2	12.958780898444754	17.791009839625392
3	3.4801714658713583	7.16685064105818
4	1.278902804921789	3.511591493767558
5	0.5868779547754793	2.014300486653584
6	0.2990055363291941	1.231507148849223
7	0.1890986176842458	0.9086420921734297
8	0.12926176765702999	0.7098499151411963
9	0.08931369434339728	0.5517813537385446
>10	0.41499958854897284	4.846277220549987
>50	0.021163952151714415	1.002310136046109
>100	0.01539736543652463	2.0827722947521052
>500	0.0022441399564201636	1.0670550632038718
>1k	0.0012540782109401087	1.8341240717396796
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3970	0.17952923545417504	No Hit
GTCCTACAGTGGACATTTCTAAATT	3650	0.16505836509011054	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3287	0.1486429715208749	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3279	0.14828119976177329	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3184	0.14398516012244164	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2966	0.13412687968692272	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2598	0.11748537876824856	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCAT	40	0.005275072	14.252021	10
TAGGACC	680	0.0	11.892027	4
GGCGAGG	795	0.0	11.699376	19
AATCCCG	195	0.0	11.194301	19
TGGCGAG	1690	0.0	10.557836	18
ACACCGT	130	3.784644E-7	10.242189	6
CCTATAC	200	2.5465852E-11	9.981611	3
TTATACT	305	0.0	9.981506	4
TGTACCG	125	2.5241752E-6	9.894167	5
GTAGGAC	2035	0.0	9.763224	3
GTATAGG	195	1.6189006E-10	9.749385	1
TGTAGGA	2135	0.0	9.661481	2
AGGACGT	1900	0.0	9.563694	5
GGACGTG	1875	0.0	9.535966	6
GTCCTAG	340	0.0	9.50565	1
GATATAC	490	0.0	9.50565	1
AATGCCG	190	1.0713848E-9	9.490819	18
GGTATCA	1415	0.0	9.472061	1
ATTTAGA	705	0.0	9.438234	1
AGGACCT	1355	0.0	9.408305	5
>>END_MODULE