FastQCFastQC Report
Thu 26 May 2016
SRR1512963_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512963_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147200
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC30810.26856694560669453No Hit
GTATCAACGCAGAGTACTTTTTTTT29960.26115760111576014No Hit
CCCATGTACTCTGCGTTGATACCAC25470.22201882845188284No Hit
GGTATCAACGCAGAGTACTTTTTTT17590.15332984658298465No Hit
TATCAACGCAGAGTACTTTTTTTTT17310.15088912133891214No Hit
GTCCTACAGTGGACATTTCTAAATT17220.15010460251046023No Hit
CTGTAGGACGTGGAATATGGCAAGA16150.14077754532775452No Hit
GTCCTAAAGTGTGTATTTCTCATTT15350.13380404463040446No Hit
CTTTAGGACGTGAAATATGGCGAGG13500.11767782426778244No Hit
GAGTACATGGGAAGCAGTGGTATCA12510.10904811715481172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAC400.00529247314.2441133
CGAAATC801.988792E-613.06793113
TAGGACC3500.013.0231894
TAGTCCC751.4840725E-512.6614344
GTATAAG1459.276846E-1111.8279451
ACTATAC752.0808798E-411.3952913
GTCCTAC8000.011.1954781
TAGGGTC600.005892158511.0787555
ACGAAAT951.3529723E-511.00457312
GTCTATA1053.342724E-610.8892191
GCATTAG700.001454634410.8892191
GTGTAGG1252.1480082E-710.6714351
ACCATCC1451.4530087E-810.4770578
TCCTACA8900.010.4563532
GTAGGAC10750.010.4235993
TGACGAA1106.075754E-610.35845210
TGTAGGA10750.010.3352632
AGGACGT9500.010.2957455
TAGAAAT3250.010.2265424
GTCTAGG1409.264295E-810.2086431
Produced by FastQC (version 0.11.5)