##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512963_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1147200
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.256231694560668	31.0	30.0	34.0	23.0	34.0
2	29.327200139470015	31.0	30.0	34.0	23.0	34.0
3	29.46183141562064	31.0	30.0	34.0	23.0	34.0
4	32.885607566248254	35.0	35.0	37.0	26.0	37.0
5	33.02460251046025	37.0	35.0	37.0	26.0	37.0
6	32.978019525801955	37.0	35.0	37.0	26.0	37.0
7	32.88015341701534	37.0	35.0	37.0	26.0	37.0
8	32.84788877266388	37.0	35.0	37.0	26.0	37.0
9	34.151389470013946	39.0	35.0	39.0	25.0	39.0
10	34.040478556485354	39.0	34.0	39.0	25.0	39.0
11	34.12368636680613	39.0	35.0	39.0	25.0	39.0
12	34.01543322873082	39.0	35.0	39.0	25.0	39.0
13	34.013511157601116	39.0	35.0	39.0	25.0	39.0
14	35.02660826359833	40.0	35.0	41.0	24.0	41.0
15	34.9619560669456	40.0	35.0	41.0	23.0	41.0
16	34.850348675034866	39.0	35.0	41.0	22.0	41.0
17	34.807526150627616	39.0	34.0	41.0	22.0	41.0
18	34.4892050209205	39.0	34.0	41.0	17.0	41.0
19	34.74182357043236	39.0	34.0	41.0	19.0	41.0
20	34.82810669456067	39.0	35.0	41.0	21.0	41.0
21	34.779035041841006	39.0	35.0	41.0	19.0	41.0
22	34.693154637377965	39.0	35.0	41.0	18.0	41.0
23	34.41834030683403	39.0	34.0	41.0	17.0	41.0
24	34.434350592747556	39.0	34.0	41.0	17.0	41.0
25	34.35924947698745	39.0	34.0	41.0	16.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6754505636245263
1101	2	0.981131041267929
1101	3	0.9917860715920526
1101	4	0.9614183213950867
1101	5	0.7666299530890939
1101	6	1.0594496290467177
1101	7	1.0377432973417697
1101	8	0.8416275402836817
1101	9	0.9204505668556777
1101	10	0.9637736283807143
1101	11	1.1821393315428423
1101	12	1.4064971734018528
1101	13	1.1728905987208407
1101	14	1.110314049222751
1101	15	0.8283072253659256
1101	16	1.4665485979923645
1101	17	1.412837440535938
1101	18	1.657358052099859
1101	19	1.1172144914661786
1101	20	1.349510373924467
1101	21	1.2018313010955382
1101	22	1.1478510018080854
1101	23	1.5538453082385217
1101	24	1.2436018489794662
1101	25	1.441712136309043
1102	1	0.5866757760891161
1102	2	0.6739934770039504
1102	3	0.5175887155958314
1102	4	0.6759931299146515
1102	5	0.5786381369103069
1102	6	0.5531452013895972
1102	7	0.7703041513969566
1102	8	0.7277861936254126
1102	9	0.6860365494388247
1102	10	0.7610342122648817
1102	11	0.9858836920506562
1102	12	0.9131734140901315
1102	13	0.8136913410353941
1102	14	0.7403000816432694
1102	15	0.9916387225855274
1102	16	0.9726326920013904
1102	17	0.8658116169077772
1102	18	1.0545848646064115
1102	19	1.1424613831879284
1102	20	1.1365716738929876
1102	21	0.9545995704271917
1102	22	1.2210660369565502
1102	23	1.3628898077663791
1102	24	1.1275427392323252
1102	25	0.9593781734510003
1103	1	0.5348962649819917
1103	2	0.716380643245472
1103	3	0.6991767201591728
1103	4	0.793798495687021
1103	5	0.4243096916511817
1103	6	0.6574364733570945
1103	7	0.5174546263178073
1103	8	0.47000287546048725
1103	9	0.6665122393879344
1103	10	0.8416566726240546
1103	11	0.8431603638873995
1103	12	0.9212082928923806
1103	13	0.8178938457461484
1103	14	1.0116228099065125
1103	15	0.9509714620007514
1103	16	1.0390620050646007
1103	17	0.889540033966469
1103	18	1.214793740050247
1103	19	1.1279218192690905
1103	20	1.0115735966494555
1103	21	0.8220838358701315
1103	22	1.0571542530231
1103	23	1.245610956805642
1103	24	1.0677309129982362
1103	25	0.9129916737650561
1104	1	0.46786127791024157
1104	2	0.49958755581819503
1104	3	0.5384750596872934
1104	4	0.7984925608130737
1104	5	0.5090654689621061
1104	6	0.31011100470809083
1104	7	0.5095108237967949
1104	8	0.5297682479556443
1104	9	0.7325372070673168
1104	10	0.6566059696505562
1104	11	0.8407868183153226
1104	12	0.7735813334547643
1104	13	0.823663581525075
1104	14	0.7800809143021183
1104	15	0.9742852346251851
1104	16	0.7801233334421056
1104	17	0.7455920701655643
1104	18	1.0379797452215485
1104	19	0.8610140946407796
1104	20	0.8296691040831945
1104	21	0.9509219095611456
1104	22	0.8219788126546419
1104	23	1.125087867762332
1104	24	0.9323832643233771
1104	25	0.7199239418646002
1105	1	0.5188712513671767
1105	2	0.6242133654806601
1105	3	0.5872519617750811
1105	4	0.726582091654528
1105	5	0.2955169932074142
1105	6	0.13304995566285527
1105	7	0.4575952416321485
1105	8	0.52905917428874
1105	9	0.34110749666402285
1105	10	0.5524077335757624
1105	11	0.4849178796416851
1105	12	0.6600425761257043
1105	13	0.6497684489088016
1105	14	0.6997281045009913
1105	15	0.803768647686752
1105	16	0.8132662599138527
1105	17	0.5408291749908543
1105	18	0.6662456314429264
1105	19	0.6808555295508469
1105	20	0.8315484123788934
1105	21	0.8098524618153249
1105	22	0.6240519323559397
1105	23	1.0335752721874911
1105	24	0.8545906716207412
1105	25	0.8352460612489594
1106	1	0.5951721733268407
1106	2	0.6431380903511901
1106	3	0.741280742668863
1106	4	0.8876275377779521
1106	5	0.6771714206867898
1106	6	0.7497570119533279
1106	7	0.4978197602434804
1106	8	0.6678294829926514
1106	9	0.7609299657694706
1106	10	0.7631446470013188
1106	11	0.7739382004227409
1106	12	0.8323350495911583
1106	13	0.7172325736891949
1106	14	1.0525438513788217
1106	15	0.9611194063451407
1106	16	0.9419278499013899
1106	17	0.9835330844985322
1106	18	1.0796152390000984
1106	19	0.5701365942470176
1106	20	0.6784986441682506
1106	21	0.6589172631601414
1106	22	0.7201462159858778
1106	23	0.9530953541775133
1106	24	0.8328683308692888
1106	25	0.9497208340917211
1107	1	0.529776176703546
1107	2	0.5577056830077609
1107	3	0.7383573704113076
1107	4	0.7097064937636404
1107	5	0.643908184999475
1107	6	0.6132883110529761
1107	7	0.6277687792269262
1107	8	0.5735149027824207
1107	9	0.7172211794301049
1107	10	0.5875257098218327
1107	11	0.6061490701642924
1107	12	0.8062518156462772
1107	13	0.8017207657407255
1107	14	1.095777075259683
1107	15	0.9340583102382638
1107	16	0.9548836973969514
1107	17	0.9574789911464876
1107	18	1.376737530351953
1107	19	1.1756282441152806
1107	20	1.0368873796768128
1107	21	0.9456718122470207
1107	22	0.9015887654572907
1107	23	1.224573623406549
1107	24	1.2884307292902903
1107	25	1.2920224602569732
1108	1	0.31886115056912345
1108	2	0.4133516000741295
1108	3	0.3584664519175256
1108	4	0.4695995346983537
1108	5	0.3187266191462612
1108	6	0.41480989194263884
1108	7	0.5846757550325918
1108	8	0.5171201526848961
1108	9	0.6713902064404067
1108	10	0.8111986984727508
1108	11	0.6175492683584238
1108	12	0.6059744036491068
1108	13	0.633391769460971
1108	14	0.7937866142864394
1108	15	0.7590215038243073
1108	16	0.6701875427517194
1108	17	0.7228616806976547
1108	18	0.8862650480974992
1108	19	0.9448118965371819
1108	20	0.8513636225378605
1108	21	0.7312144023125029
1108	22	0.7448967241623663
1108	23	0.9956851098475852
1108	24	0.9521278719168293
1108	25	0.9793713671251609
1109	1	-0.007736692914150467
1109	2	0.12773050488012316
1109	3	0.2151444466616148
1109	4	0.30796426216056005
1109	5	0.20870934466764623
1109	6	0.24251322269044806
1109	7	0.11046094403688045
1109	8	0.18366654819811146
1109	9	0.27765083891404174
1109	10	0.4464907726953129
1109	11	-0.06340493977355521
1109	12	0.4007603744158885
1109	13	0.27645537489100036
1109	14	0.13287190027079276
1109	15	0.3583998179585208
1109	16	0.3659372395554641
1109	17	0.20702084033807466
1109	18	-1.3781776401031962
1109	19	0.03179724512968818
1109	20	0.12601867479629192
1109	21	0.28436952065901266
1109	22	0.25622220690299713
1109	23	-0.00998503399853945
1109	24	0.36202356263802926
1109	25	0.39244020160305126
1110	1	0.15714796058765046
1110	2	0.09884891505277693
1110	3	0.3040601135298715
1110	4	0.17854393399250057
1110	5	0.22029348647063074
1110	6	0.2421492755478809
1110	7	0.30627687973110085
1110	8	0.23300824446943125
1110	9	0.5074393995061115
1110	10	0.6125707782963588
1110	11	0.5619045360097772
1110	12	0.125437982432139
1110	13	0.4586610152870847
1110	14	0.5604714666727375
1110	15	0.6855870920004534
1110	16	0.7648082187845233
1110	17	0.19936105064278564
1110	18	0.6543126395506462
1110	19	0.615207230680447
1110	20	0.6230627359394063
1110	21	0.5840757676336636
1110	22	0.6713757467265395
1110	23	0.7604726582023673
1110	24	0.7723299059409783
1110	25	0.7703154142073316
1111	1	0.14693804940382194
1111	2	0.45678005845918435
1111	3	0.3463082558923958
1111	4	0.38817378846625417
1111	5	0.33081273655515275
1111	6	0.4506217311829843
1111	7	0.32079063648978945
1111	8	0.14593680902341077
1111	9	0.5830090297554378
1111	10	0.601613740250265
1111	11	0.3611859122532408
1111	12	0.5152895195022182
1111	13	0.36317746194925604
1111	14	0.42424204505134355
1111	15	0.6348503299039123
1111	16	0.5391610995724463
1111	17	0.5522615809733011
1111	18	0.8248266085402562
1111	19	0.3286467535063693
1111	20	0.3965216556840403
1111	21	0.4229375929676138
1111	22	0.2731275155231643
1111	23	0.5099211045980212
1111	24	0.5724102328753062
1111	25	0.24513458606255512
1112	1	0.033902423580762786
1112	2	0.4599621357650534
1112	3	0.44373720215368806
1112	4	0.43303778002220383
1112	5	0.42004595114404
1112	6	0.389100532685589
1112	7	0.296673315981856
1112	8	0.5188261078337248
1112	9	0.7353261645540314
1112	10	0.6332173130391183
1112	11	0.450308348481002
1112	12	0.4379649787345343
1112	13	0.38044768397896433
1112	14	0.5327892801977043
1112	15	0.5420249091328415
1112	16	0.7988840789875837
1112	17	0.8116478688526527
1112	18	0.712262724277501
1112	19	0.5572179864819802
1112	20	0.4648382975811671
1112	21	0.5710961827715266
1112	22	0.5160787755336713
1112	23	0.7833880689970911
1112	24	0.5604237652611701
1112	25	0.5834274292825441
1113	1	0.13784024922849625
1113	2	0.2112511486201818
1113	3	0.0386926720700842
1113	4	0.30008479399013055
1113	5	0.16681074612702673
1113	6	0.22120449615872673
1113	7	0.0667148709561971
1113	8	0.3316612602281168
1113	9	0.3505132826326758
1113	10	0.21588703004768917
1113	11	0.027605971652334915
1113	12	0.3314252902942556
1113	13	0.22399495562876837
1113	14	0.23515796201309058
1113	15	-0.0902120592247897
1113	16	-0.26887177135657225
1113	17	-0.0684247527798334
1113	18	-0.03863705014524754
1113	19	-0.05240634110108289
1113	20	-0.3090684855472503
1113	21	-0.21506678321325978
1113	22	0.0469146062288317
1113	23	-0.47196079122829104
1113	24	0.183826831615157
1113	25	0.31945467476587197
1114	1	-1.2598660302619358
1114	2	-1.0128671190102523
1114	3	-0.9851062137063451
1114	4	-1.1615701146392006
1114	5	-1.1268076985363322
1114	6	-1.426125738897646
1114	7	-1.6126185820048278
1114	8	-1.1255581907530292
1114	9	-1.3091911928079156
1114	10	-1.6024639383344734
1114	11	-2.0957092047663934
1114	12	-1.7087735285227765
1114	13	-1.570258459179854
1114	14	-1.872908597550662
1114	15	-1.8936016376455598
1114	16	-2.398555315965261
1114	17	-4.390597886166315
1114	18	-3.542630828702343
1114	19	-2.4773163794702597
1114	20	-1.4916619938581661
1114	21	-2.3773298059722308
1114	22	-2.6694605783310053
1114	23	-2.4386719770598795
1114	24	-1.7840419930066247
1114	25	-1.6910510854279792
1115	1	-1.316330354114701
1115	2	-0.9574761707986426
1115	3	-1.0502070267105026
1115	4	-0.922000246865398
1115	5	-0.6449692510394911
1115	6	-0.7366303415226199
1115	7	-1.9761676127289043
1115	8	-1.4898485654387166
1115	9	-1.2101428133180079
1115	10	-0.9563895485763183
1115	11	-1.747443405923221
1115	12	-1.5582825370584672
1115	13	-1.2186793690505127
1115	14	-1.3055180794224057
1115	15	-2.579298372677556
1115	16	-1.5006307736008253
1115	17	-1.4498122387320294
1115	18	-1.3997176126807602
1115	19	-1.6912507746533265
1115	20	-1.3508234412841418
1115	21	-2.2153933434566824
1115	22	-2.4115608512873195
1115	23	-1.6070184531865905
1115	24	-1.5768856870316483
1115	25	-2.1387843506647783
1116	1	-1.2134493307230692
1116	2	-2.0848751144519753
1116	3	-1.530995227932511
1116	4	-1.1446045993069163
1116	5	-1.7378162522287468
1116	6	-1.3627674572947726
1116	7	-0.9470508272106741
1116	8	-1.7524737172733964
1116	9	-1.5175960375934636
1116	10	-2.858056249238583
1116	11	-1.2831531201798825
1116	12	-1.159317303912232
1116	13	-1.687445544672947
1116	14	-1.5281593835999274
1116	15	-1.1212620943499871
1116	16	-1.2301813133770025
1116	17	-1.544852990584566
1116	18	-1.0091747148192027
1116	19	-1.556140742729987
1116	20	-1.8427494869665395
1116	21	-2.2459358747570164
1116	22	-1.5640884534950814
1116	23	-1.4956544936632241
1116	24	-2.1088818996265104
1116	25	-1.9044095400424226
1201	1	0.14001241929462793
1201	2	0.132972154288165
1201	3	0.28087541867109067
1201	4	0.45313748901631357
1201	5	0.4307193001681284
1201	6	0.46449449349879757
1201	7	0.32924291551664453
1201	8	-0.014394557845371025
1201	9	0.37100421328560884
1201	10	0.514514607762159
1201	11	0.11539152703730338
1201	12	-0.39971464408574064
1201	13	0.0022803944443978708
1201	14	0.603172726198693
1201	15	0.2679072635484374
1201	16	0.800908086346702
1201	17	0.9854975684473501
1201	18	1.237911221248389
1201	19	0.6282217938716741
1201	20	0.599904131083683
1201	21	0.6015007837224928
1201	22	0.83315550734455
1201	23	1.0615593689487142
1201	24	0.886458139548381
1201	25	0.862244782357152
1202	1	0.8972574389738845
1202	2	0.982838686238221
1202	3	0.6189753766041655
1202	4	1.0450479613760422
1202	5	0.8593415859069324
1202	6	0.8287716690856399
1202	7	0.9886922549634463
1202	8	1.0586485457077757
1202	9	1.2661640836144699
1202	10	1.4923500754958
1202	11	1.1118318454653675
1202	12	1.1941378220158114
1202	13	1.336016789123093
1202	14	1.5679069394159058
1202	15	1.2598345114558143
1202	16	1.4156601956157004
1202	17	1.4446907286339794
1202	18	1.7879522957286156
1202	19	1.6843017453634488
1202	20	1.3892419899731578
1202	21	1.4440894812292768
1202	22	1.5081715305537102
1202	23	1.6458456110147424
1202	24	1.7401875294754987
1202	25	1.5054426499417417
1203	1	0.5523720281298381
1203	2	0.7329784291113342
1203	3	0.6944365280854399
1203	4	0.8113978775303181
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2211	16	0.25207597319365505
2211	17	0.32013114556457367
2211	18	0.18923230045388095
2211	19	-0.09193769009072383
2211	20	-0.25965346451496174
2211	21	-0.19270671690319574
2211	22	-0.21232118881953
2211	23	0.2975534581192747
2211	24	0.1637085699684775
2211	25	0.3377327441485818
2212	1	-0.22295252007591415
2212	2	-0.3017836878885376
2212	3	-0.2639040899351137
2212	4	-0.3863647590421806
2212	5	-0.2531326509246128
2212	6	-0.467461367853268
2212	7	-0.43599834077211597
2212	8	-0.2732795612010577
2212	9	-0.22080412395848725
2212	10	-0.1358883270769411
2212	11	-0.48476187583193564
2212	12	-0.2759805962179911
2212	13	-0.4344857065491894
2212	14	-0.5042616360440135
2212	15	-0.5657925462118172
2212	16	-0.43177290633133936
2212	17	-0.5410268725657161
2212	18	-3.0653281528275365
2212	19	-1.2688828531251133
2212	20	-0.7031542124680925
2212	21	-0.60976877640487
2212	22	-0.5110554266468057
2212	23	-0.49500289293622046
2212	24	-1.176809820950382
2212	25	-0.7951331891926685
2213	1	-0.31855913166633343
2213	2	-0.08157062334682408
2213	3	-0.4925860429047155
2213	4	-0.5095663405521833
2213	5	-0.4633023337684037
2213	6	-0.4260830989721569
2213	7	-0.18547025554629926
2213	8	-0.6109831989181203
2213	9	-0.5026539781325283
2213	10	-0.5003470626011222
2213	11	-0.4470606787167313
2213	12	-0.5789361650612648
2213	13	-0.32440632597393915
2213	14	-0.5027390055633347
2213	15	-0.503465371330492
2213	16	-0.9658934354538218
2213	17	-0.6335284284493952
2213	18	-0.48521722747334906
2213	19	-0.33459087172249724
2213	20	-1.0273921345396744
2213	21	-0.5869540302884744
2213	22	-0.5364300590311473
2213	23	-0.8511379558427379
2213	24	-0.5307937983992161
2213	25	-0.7073721973320772
2214	1	-1.4592892293599462
2214	2	-3.4039159072763603
2214	3	-1.61605847508228
2214	4	-1.1877747546596922
2214	5	-1.1867116864227611
2214	6	-1.1938475511121638
2214	7	-1.5007353408709108
2214	8	-1.1564799421988
2214	9	-1.4405700282074747
2214	10	-1.6154375641948349
2214	11	-1.3810501877637051
2214	12	-1.7813535762840047
2214	13	-1.548428792478866
2214	14	-2.5967064686355315
2214	15	-3.0105861893253625
2214	16	-2.1285025701970994
2214	17	-2.723175899932194
2214	18	-2.0994273237014767
2214	19	-3.9832133965282033
2214	20	-2.353957056271689
2214	21	-2.1737340914246417
2214	22	-2.421698600986197
2214	23	-2.598376394847545
2214	24	-2.7907187082799254
2214	25	-2.7060819343573677
2215	1	-1.0046630727391097
2215	2	-2.7327683695064806
2215	3	-1.4091608981049148
2215	4	-1.0257870658103023
2215	5	-1.0588119661028266
2215	6	-1.6555545797074913
2215	7	-2.2039023255538517
2215	8	-1.9597508267196844
2215	9	-1.7712371435820344
2215	10	-3.170727634245548
2215	11	-2.2922083115548055
2215	12	-2.58247589057121
2215	13	-1.820238610033364
2215	14	-3.1251341831004815
2215	15	-2.94595624264754
2215	16	-2.4965406275968576
2215	17	-2.8617942934707976
2215	18	-1.5352345404927377
2215	19	-2.21008980146312
2215	20	-2.668545181631089
2215	21	-2.3705066618674238
2215	22	-1.8132180217609388
2215	23	-2.3167039341857247
2215	24	-2.2895526122999996
2215	25	-2.036255035408125
2216	1	-2.1459131996362224
2216	2	-2.017528343619471
2216	3	-2.7144534593962213
2216	4	-3.478059158597471
2216	5	-2.571588919049379
2216	6	-2.3748166095536902
2216	7	-2.4835553254015785
2216	8	-3.014403192081847
2216	9	-3.178190788067088
2216	10	-2.652318987476349
2216	11	-3.1285227752876104
2216	12	-3.399064198271329
2216	13	-4.1741774963843135
2216	14	-3.377480827940847
2216	15	-3.262482370762868
2216	16	-2.42051402996222
2216	17	-2.5539263552950757
2216	18	-2.5502017375893296
2216	19	-2.967785159566766
2216	20	-2.81539400910178
2216	21	-3.4294471978808225
2216	22	-3.8700742727126674
2216	23	-3.7517713165521016
2216	24	-3.7653346157515983
2216	25	-3.620613380162201
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	52211.0
3	3.0
4	401.0
5	2465.0
6	3681.0
7	3064.0
8	2843.0
9	2569.0
10	2343.0
11	2262.0
12	2089.0
13	2133.0
14	2159.0
15	2107.0
16	2167.0
17	2340.0
18	2485.0
19	3119.0
20	4000.0
21	5238.0
22	6578.0
23	7809.0
24	8878.0
25	10198.0
26	11496.0
27	12781.0
28	14233.0
29	17352.0
30	21135.0
31	26212.0
32	33165.0
33	42684.0
34	55193.0
35	73526.0
36	100717.0
37	156653.0
38	448911.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62867618433565	18.39052304020674	12.604160561016982	25.37664021444062
2	15.083654679621675	19.933149507559705	40.61712439981932	24.366071412999307
3	18.8560999515171	24.07655764407968	29.48990055772779	27.577441846675434
4	12.622712092013359	16.409891959295074	37.14437691294832	33.82301903574324
5	13.359779477873227	37.634392661587306	35.59076088330937	13.415066977230097
6	32.920163872825064	36.321422997819504	17.43010164892889	13.328311480426544
7	28.423152404857575	31.532767040020364	21.584824989908377	18.459255565213674
8	25.698526655006393	36.21779837023526	20.179891430394957	17.903783544363396
9	26.554386075044377	14.998587555843857	19.898896898551634	38.54812947056013
10	15.288401262117892	28.590945986829542	34.1841614492559	21.93649130179666
11	34.14407675165567	22.569687838935913	24.21602271023181	19.070212699176615
12	23.432578034339382	24.82233515815244	31.12245120374415	20.622635603764028
13	28.94351614914143	21.772155209618425	25.53329874930796	23.751029891932184
14	21.17511448411522	22.25063737919441	27.095068027804032	29.47918010888634
15	23.4997964007844	30.487216846679765	23.03906053131112	22.973926221224716
16	22.335178512109913	28.140473062104547	27.395717174112626	22.12863125167291
17	22.07774996447046	28.630530292527983	27.485302105702775	21.80641763729878
18	22.052077670614537	27.1825336229155	29.32080532696229	21.44458337950767
19	23.382581818023287	27.22963105052293	27.563573268928117	21.824213862525667
20	23.729123889986006	27.143672974728517	27.735244497338584	21.391958637946892
21	22.908937451008942	26.787154512234356	27.30673218861742	22.99717584813928
22	23.367219718039706	27.669686216727552	26.764518688370227	22.198575376862518
23	22.217707798395907	27.63777735733573	27.60630232941363	22.53821251485473
24	23.15157422648774	27.007714198030296	27.80755656613565	22.033155009346316
25	22.832807147409657	27.00009503606575	27.426187929024625	22.740909887499967
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	211.0
1	211.0
2	360.5
3	510.0
4	510.0
5	510.0
6	956.5
7	1403.0
8	1403.0
9	1403.0
10	1884.5
11	2366.0
12	2366.0
13	2366.0
14	3626.0
15	4886.0
16	4886.0
17	4886.0
18	8848.5
19	12811.0
20	12811.0
21	12811.0
22	19845.0
23	26879.0
24	26879.0
25	26879.0
26	38170.0
27	49461.0
28	49461.0
29	49461.0
30	64227.5
31	78994.0
32	78994.0
33	78994.0
34	90549.0
35	102104.0
36	102104.0
37	102104.0
38	111503.0
39	120902.0
40	120902.0
41	120902.0
42	129118.5
43	137335.0
44	137335.0
45	137335.0
46	146426.5
47	155518.0
48	155518.0
49	155518.0
50	150185.0
51	144852.0
52	144852.0
53	144852.0
54	130473.0
55	116094.0
56	116094.0
57	116094.0
58	100024.0
59	83954.0
60	83954.0
61	83954.0
62	69347.5
63	54741.0
64	54741.0
65	54741.0
66	42668.0
67	30595.0
68	30595.0
69	30595.0
70	22520.0
71	14445.0
72	14445.0
73	14445.0
74	10141.5
75	5838.0
76	5838.0
77	5838.0
78	4037.5
79	2237.0
80	2237.0
81	2237.0
82	1511.0
83	785.0
84	785.0
85	785.0
86	491.0
87	197.0
88	197.0
89	197.0
90	128.5
91	60.0
92	60.0
93	60.0
94	34.0
95	8.0
96	8.0
97	8.0
98	11.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.360268479776848
2	0.040271966527196654
3	0.035216178521617854
4	0.03573919107391911
5	0.04070781032078103
6	0.01856694560669456
7	0.018218270571827056
8	0.024930264993026502
9	0.02196652719665272
10	0.020833333333333336
11	0.0198744769874477
12	0.020658995815899583
13	0.02048465829846583
14	0.027545327754532774
15	0.02955020920502092
16	0.02153068340306834
17	0.023274058577405856
18	0.10294630404463041
19	0.025714783821478383
20	0.022228033472803346
21	0.026760808926080893
22	0.02004881450488145
23	0.02257670850767085
24	0.019525801952580194
25	0.023274058577405856
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1147200.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.60495202459865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.24594041487985	60.440675100649486
2	11.537942075511618	16.75423461710238
3	2.8925784658530596	6.300465622219457
4	1.0137771777652245	2.9442097342110856
5	0.4455503294198782	1.6174580146037187
6	0.2360636242979763	1.028363287014447
7	0.13849639900420638	0.7038867083696053
8	0.0935254107483208	0.5432326368370172
9	0.06343840508970128	0.4145348122249334
>10	0.29933481720052335	3.706021962579194
>50	0.016872937330006676	0.8257174190450061
>100	0.013232080349000815	1.9656186579599455
>500	0.002044950494874671	1.0449043198258983
>1k	0.0012029120558086298	1.710677107357896
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3081	0.26856694560669453	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2996	0.26115760111576014	No Hit
CCCATGTACTCTGCGTTGATACCAC	2547	0.22201882845188284	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1759	0.15332984658298465	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1731	0.15088912133891214	No Hit
GTCCTACAGTGGACATTTCTAAATT	1722	0.15010460251046023	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1615	0.14077754532775452	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1535	0.13380404463040446	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1350	0.11767782426778244	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1251	0.10904811715481172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.716875871687587E-5	0.0
5	0.0	0.0	0.0	8.716875871687587E-5	0.0
6	0.0	0.0	0.0	8.716875871687587E-5	0.0
7	0.0	0.0	0.0	8.716875871687587E-5	0.0
8	0.0	0.0	0.0	8.716875871687587E-5	0.0
9	0.0	0.0	0.0	8.716875871687587E-5	0.0
10	0.0	0.0	0.0	8.716875871687587E-5	0.0
11	0.0	0.0	0.0	8.716875871687587E-5	0.0
12	0.0	0.0	0.0	8.716875871687587E-5	0.0
13	0.0	0.0	0.0	8.716875871687587E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGGAC	40	0.005292473	14.244113	3
CGAAATC	80	1.988792E-6	13.067931	13
TAGGACC	350	0.0	13.023189	4
TAGTCCC	75	1.4840725E-5	12.661434	4
GTATAAG	145	9.276846E-11	11.827945	1
ACTATAC	75	2.0808798E-4	11.395291	3
GTCCTAC	800	0.0	11.195478	1
TAGGGTC	60	0.0058921585	11.078755	5
ACGAAAT	95	1.3529723E-5	11.004573	12
GTCTATA	105	3.342724E-6	10.889219	1
GCATTAG	70	0.0014546344	10.889219	1
GTGTAGG	125	2.1480082E-7	10.671435	1
ACCATCC	145	1.4530087E-8	10.477057	8
TCCTACA	890	0.0	10.456353	2
GTAGGAC	1075	0.0	10.423599	3
TGACGAA	110	6.075754E-6	10.358452	10
TGTAGGA	1075	0.0	10.335263	2
AGGACGT	950	0.0	10.295745	5
TAGAAAT	325	0.0	10.226542	4
GTCTAGG	140	9.264295E-8	10.208643	1
>>END_MODULE