FastQCFastQC Report
Thu 26 May 2016
SRR1512963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512963_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147200
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36030.31406903765690375No Hit
CCCATGTACTCTGCGTTGATACCAC30220.26342398884239887No Hit
GTACATGGGAAGCAGTGGTATCAAC29580.25784518828451886No Hit
TATCAACGCAGAGTACTTTTTTTTT27190.23701185495118549No Hit
GGTATCAACGCAGAGTACTTTTTTT22570.19673988842398882No Hit
GTCCTACAGTGGACATTTCTAAATT16570.14443863319386332No Hit
CTGTAGGACGTGGAATATGGCAAGA15630.13624476987447698No Hit
CTTTAGGACGTGAAATATGGCGAGG14170.1235181311018131No Hit
GTCCTAAAGTGTGTATTTCTCATTT14170.1235181311018131No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12470.1086994421199442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC550.003050491412.099943
TAGGACC3100.011.35759454
CTACACT1701.0913936E-1111.1950854
TAGAACG600.00580174111.1017924
TTGACCC600.00580174111.1017924
ATTAGGC600.00584150311.0916133
ACGGCCA700.001470326210.8752275
TAGGACT1752.0008883E-1110.8752264
CCTACAC1408.240022E-910.8652533
GTAATAC1158.7226726E-710.7471513
GTATAGG1355.6383215E-810.5606731
GACGTGG4250.010.5123537
CGTGGAA4150.010.303959
GTAGGAC9000.010.2465373
GTCCTAG1409.604446E-810.1835071
CTAGGAC1502.41198E-810.1409023
ATGGAAC951.6306332E-410.0074693
CCCCTAA951.6349503E-410.00484851
GTCCTAA5350.09.9487471
TGTAGGA9150.09.8703242