##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512963_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1147200
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.339415969316597	31.0	31.0	34.0	26.0	34.0
2	30.493021269177127	31.0	31.0	34.0	27.0	34.0
3	30.593401324965132	31.0	31.0	34.0	27.0	34.0
4	34.045258019525804	37.0	35.0	37.0	30.0	37.0
5	33.68350418410042	37.0	35.0	37.0	28.0	37.0
6	33.51636506276151	37.0	35.0	37.0	28.0	37.0
7	33.38131363319386	37.0	35.0	37.0	27.0	37.0
8	33.26101290097629	37.0	35.0	37.0	27.0	37.0
9	34.69768392608089	39.0	35.0	39.0	27.0	39.0
10	34.59253748256625	39.0	35.0	39.0	27.0	39.0
11	34.60868288005579	39.0	35.0	39.0	27.0	39.0
12	34.46529027196653	39.0	35.0	39.0	27.0	39.0
13	34.44613406555091	39.0	35.0	39.0	27.0	39.0
14	35.48850069735007	40.0	36.0	41.0	26.0	41.0
15	35.49299511854951	40.0	36.0	41.0	26.0	41.0
16	35.46718880753138	40.0	36.0	41.0	26.0	41.0
17	35.450472454672244	40.0	36.0	41.0	26.0	41.0
18	35.38190027894003	40.0	36.0	41.0	25.0	41.0
19	35.35905247559275	40.0	36.0	41.0	25.0	41.0
20	35.33883978382148	40.0	36.0	41.0	25.0	41.0
21	35.28406032078103	40.0	36.0	41.0	25.0	41.0
22	35.24631537656904	40.0	36.0	41.0	25.0	41.0
23	35.13757235006973	40.0	35.0	41.0	25.0	41.0
24	35.119347105997214	40.0	36.0	41.0	25.0	41.0
25	35.02119944211994	40.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5886991584939096
1101	2	0.7034362188442458
1101	3	0.6019964275282454
1101	4	0.4903236307363912
1101	5	0.7526412821707353
1101	6	0.7320050187957889
1101	7	0.8229486611658459
1101	8	0.6664929417990564
1101	9	0.7520929409087458
1101	10	1.020587889359696
1101	11	1.0068876308493202
1101	12	1.2231281642562806
1101	13	1.0954548906609887
1101	14	1.161806939842073
1101	15	1.0483604428593623
1101	16	1.2362204035798428
1101	17	1.109155296003422
1101	18	0.7545440700601986
1101	19	1.001283420597943
1101	20	0.9970718623208441
1101	21	1.1788465154506298
1101	22	1.1446720241146338
1101	23	1.1450463619747069
1101	24	0.9517377928145336
1101	25	1.0781098454813005
1102	1	0.5744607522406326
1102	2	0.48827123091013647
1102	3	0.5611415854340258
1102	4	0.5947555502621498
1102	5	0.6330004391322248
1102	6	0.5018471785901397
1102	7	0.483967638712798
1102	8	0.5974790004497805
1102	9	0.6377174818803084
1102	10	0.6617591331956021
1102	11	0.7030370380468867
1102	12	0.6323057820298601
1102	13	0.7268196477693678
1102	14	0.953051956104801
1102	15	0.8508664102289956
1102	16	0.8027716573832251
1102	17	0.7476307314246853
1102	18	0.8346274820841231
1102	19	0.8531428136312087
1102	20	0.9653005371713377
1102	21	0.8274982833658555
1102	22	0.8252288911800036
1102	23	0.8770422831792004
1102	24	0.7798650879567006
1102	25	0.8145458831478578
1103	1	0.6057250927992577
1103	2	0.6597061199670868
1103	3	0.7090749426949969
1103	4	0.6739926645892496
1103	5	0.7246566374581462
1103	6	0.8649366636759623
1103	7	0.8280993859856878
1103	8	0.6854121013432177
1103	9	0.7632689925259726
1103	10	0.8242397454012576
1103	11	0.6804254220341761
1103	12	0.6196816040082496
1103	13	0.7530822578471756
1103	14	1.0048865862305476
1103	15	0.72695469038743
1103	16	0.9934895505548766
1103	17	1.0255574872217892
1103	18	1.2069699121919228
1103	19	1.0937267547692215
1103	20	1.0196648738844445
1103	21	0.7221052719446845
1103	22	1.0807475226901317
1103	23	1.1484845428025992
1103	24	1.1307274442306579
1103	25	1.0348694189688104
1104	1	0.3280758119330649
1104	2	0.4323962056167332
1104	3	0.4528355478986157
1104	4	0.4517984984612582
1104	5	0.5305637358744377
1104	6	0.5099274724994913
1104	7	0.6434678410600299
1104	8	0.5222510436509111
1104	9	0.697860136675942
1104	10	0.7495892121110188
1104	11	0.7422133583625481
1104	12	0.7975535941504646
1104	13	0.8422720202906149
1104	14	0.8413290959267314
1104	15	0.9905520764572486
1104	16	0.7953515213047098
1104	17	0.6406946478552769
1104	18	0.7920564708538507
1104	19	0.7343770052540322
1104	20	0.7622008305748125
1104	21	0.7248121239162373
1104	22	0.8770215611516718
1104	23	0.6249157402815868
1104	24	0.6419866354071289
1104	25	0.9589588864865917
1105	1	0.49042737381405743
1105	2	0.5477427758171913
1105	3	0.510255039717805
1105	4	0.5114622037936414
1105	5	0.42220917056661733
1105	6	0.544147782399655
1105	7	0.6089717630947291
1105	8	0.7310891780103148
1105	9	0.6948542338156258
1105	10	0.7375767428138147
1105	11	0.7361043683855257
1105	12	0.7942316877048583
1105	13	0.7256044870127454
1105	14	0.8586644150377367
1105	15	0.7889538596954964
1105	16	0.8946908540636045
1105	17	0.5762924362634863
1105	18	0.9434190931698296
1105	19	0.9273508930204528
1105	20	0.9768393794219747
1105	21	0.9948630311343649
1105	22	1.0658951399093013
1105	23	0.8985946534025757
1105	24	0.9418796089234931
1105	25	0.9846727884159137
1106	1	0.5207107249114848
1106	2	0.5580520667759714
1106	3	0.48594177033522
1106	4	0.382419954596088
1106	5	0.4557420365910829
1106	6	0.4682507566781027
1106	7	0.5291388128666128
1106	8	0.6160053504315073
1106	9	0.4774415668224208
1106	10	0.6270330024717055
1106	11	0.5547662702384386
1106	12	0.7159776323848277
1106	13	0.5712118631792578
1106	14	0.753408015835376
1106	15	0.7899108583520302
1106	16	0.8715464683436167
1106	17	0.8461883771827274
1106	18	0.7710055425580506
1106	19	0.7425709629639101
1106	20	0.8283455974672407
1106	21	0.6921790320907562
1106	22	0.7486192044813293
1106	23	0.8326809887459135
1106	24	0.8527992387698617
1106	25	0.813272188778285
1107	1	0.4253735201853033
1107	2	0.4077593089643372
1107	3	0.46817742460161327
1107	4	0.4287946545531227
1107	5	0.4535946452749826
1107	6	0.5283602892569874
1107	7	0.5258831777669144
1107	8	0.700403717356636
1107	9	0.6383087548152915
1107	10	0.7510598719008215
1107	11	0.707503232827321
1107	12	0.6244278034731536
1107	13	0.6983474630699078
1107	14	0.8753142311933715
1107	15	0.7696103797853908
1107	16	0.7613995336636066
1107	17	0.7325926383184935
1107	18	0.7565346846529906
1107	19	0.8890499081345311
1107	20	1.011282428472832
1107	21	0.9796437704713199
1107	22	0.9834461183723775
1107	23	0.9167687229596737
1107	24	0.9894427832272896
1107	25	1.0856159636566929
1108	1	0.23892784645230236
1108	2	0.10860753799664025
1108	3	0.09170991364189618
1108	4	0.16255417003872452
1108	5	0.28061301933576743
1108	6	0.10737801761742816
1108	7	0.21310384137348137
1108	8	0.2963292286481689
1108	9	0.1446756291922
1108	10	0.2820575237637115
1108	11	0.30430133638151347
1108	12	0.4506393510464761
1108	13	0.2781220957157373
1108	14	0.49928167115594846
1108	15	0.36112360543224753
1108	16	0.32242344699627523
1108	17	0.19245085328588374
1108	18	0.3209105599730364
1108	19	0.38494746897161036
1108	20	0.5264152828121027
1108	21	0.631955960606156
1108	22	0.6065239267578164
1108	23	0.4942986501240796
1108	24	0.6779871325272637
1108	25	0.5705993951653738
1109	1	0.25500449763940125
1109	2	0.13436662755011497
1109	3	0.16724019800991385
1109	4	0.3040708869659099
1109	5	0.12929817173976943
1109	6	-0.04273625524118785
1109	7	0.039077587462763574
1109	8	0.20235807968318653
1109	9	0.32055967559000464
1109	10	0.01967104492761962
1109	11	0.1819324586487454
1109	12	0.2709209748047243
1109	13	0.03953221088605119
1109	14	0.32953053781400143
1109	15	0.30023953919275925
1109	16	0.1847148078338492
1109	17	0.14959402876790762
1109	18	0.2595292305128041
1109	19	0.43193655390654584
1109	20	0.5287798014787057
1109	21	0.5745368635616828
1109	22	0.4854444534722049
1109	23	0.2899744442723673
1109	24	-0.19873096684662528
1109	25	0.09650180275576759
1110	1	0.27470532463836506
1110	2	0.3280495794933991
1110	3	0.31008371763831377
1110	4	0.46934091401750777
1110	5	0.25498245875947134
1110	6	0.3345360001567599
1110	7	0.3078232795477831
1110	8	0.47343562879191126
1110	9	0.3481351599173408
1110	10	0.5395595793251502
1110	11	0.4070844654279355
1110	12	0.6217771861089361
1110	13	-0.002003332203962316
1110	14	0.5529880477395608
1110	15	0.5817055762248344
1110	16	0.5753583484661604
1110	17	0.37318854698759907
1110	18	0.3989463356663663
1110	19	0.5993178365214646
1110	20	0.6918326480523476
1110	21	0.5434323594290973
1110	22	0.6493066750345378
1110	23	0.655582284285309
1110	24	0.6955641164266524
1110	25	0.7359177007877946
1111	1	-0.250136797769521
1111	2	-0.11993472035433683
1111	3	-0.124262894934148
1111	4	-0.07961656329722899
1111	5	0.1752194170462218
1111	6	0.09748702170313095
1111	7	0.26269829774781783
1111	8	0.33519988466342454
1111	9	-0.1680894001353721
1111	10	0.07854713913848599
1111	11	0.41195652226633683
1111	12	0.09916904088376555
1111	13	0.20140704588788338
1111	14	0.4269796424081207
1111	15	0.4734548525420834
1111	16	0.15197062479081325
1111	17	0.4384495539986233
1111	18	0.34465593960892704
1111	19	0.40405922327776267
1111	20	0.2690902544330953
1111	21	0.09301665828971295
1111	22	0.05990871985474655
1111	23	0.16897072362304755
1111	24	0.29308607368926687
1111	25	0.3667407865759742
1112	1	0.2395496123394416
1112	2	0.031506767511075395
1112	3	0.17791931430999597
1112	4	0.14449055066507555
1112	5	0.3644070755899733
1112	6	0.3540279238124029
1112	7	0.18578575290747779
1112	8	0.5727416754889845
1112	9	0.28130418169001103
1112	10	0.4996038651275896
1112	11	0.41719235570547397
1112	12	0.4606917330219815
1112	13	0.4746742358267184
1112	14	0.5151505049608076
1112	15	0.5004318728780035
1112	16	0.3399565248214387
1112	17	0.44394615223164635
1112	18	0.43929364458001174
1112	19	0.6389199288238956
1112	20	0.8156501506748484
1112	21	0.8821855218530956
1112	22	0.756244072092592
1112	23	0.6736003878276975
1112	24	0.5789994226062518
1112	25	0.647791089909532
1113	1	0.03524710950614818
1113	2	-0.48329515538712897
1113	3	-0.041870653838834926
1113	4	-0.10879685454480637
1113	5	0.18665214252087736
1113	6	0.057321725808925805
1113	7	0.24668074784436556
1113	8	0.12723716816828556
1113	9	0.17858959061967994
1113	10	-0.02149979781727751
1113	11	0.13732418985730988
1113	12	0.05805716213171053
1113	13	0.0814344927120132
1113	14	0.1214457878737818
1113	15	0.28648983500661274
1113	16	-0.13819482797824634
1113	17	0.2126015356103821
1113	18	0.22063005400332258
1113	19	0.09011938666605346
1113	20	0.4919736518654929
1113	21	0.1350782573966569
1113	22	-0.03736843471153861
1113	23	0.22097975710453
1113	24	-0.10861018271201317
1113	25	-0.08588865557077696
1114	1	-0.48659297315738925
1114	2	-0.32823327816367964
1114	3	-0.5672074379092429
1114	4	-0.23091555331665603
1114	5	-0.4686686604137691
1114	6	-0.8967830897275846
1114	7	-0.8831229262512679
1114	8	-1.0955334814582613
1114	9	-0.955563132194925
1114	10	-0.9416245277517348
1114	11	-0.7444668896100026
1114	12	-0.9376377463049366
1114	13	-0.7833761783993438
1114	14	-2.04055740594476
1114	15	-1.4253502356138696
1114	16	-1.2649723830932942
1114	17	-1.4068285508346818
1114	18	-1.3261813059708558
1114	19	-1.4522408596867038
1114	20	-0.8249502143409657
1114	21	-0.9058413383420287
1114	22	-0.8431271833898109
1114	23	-0.9259118096248358
1114	24	-1.4061335883919952
1114	25	-1.3116104561522164
1115	1	-1.6767875614381076
1115	2	-1.3456904469933875
1115	3	-1.146372709655946
1115	4	-2.32801878309602
1115	5	-1.6171283633141442
1115	6	-1.5112607095094646
1115	7	-1.263029480868184
1115	8	-1.310514677529838
1115	9	-1.619426417005407
1115	10	-1.527040364593752
1115	11	-1.4481477066706248
1115	12	-1.9733704030715415
1115	13	-1.5155772032684212
1115	14	-2.260124732316932
1115	15	-2.0992679406304546
1115	16	-1.633124038792019
1115	17	-1.7540967273545718
1115	18	-1.7116080781708547
1115	19	-1.4388264132846231
1115	20	-1.687459164228926
1115	21	-1.81405489183242
1115	22	-2.143311923739205
1115	23	-2.2374852060370642
1115	24	-1.6133186073181065
1115	25	-1.547009004132164
1116	1	-0.829264318057124
1116	2	-0.7763174056201549
1116	3	-0.7000117018372549
1116	4	-0.677532542592246
1116	5	-0.712277901077492
1116	6	-1.4635335516684975
1116	7	-0.7299096398848093
1116	8	-0.9530382623448617
1116	9	-0.9495674159615959
1116	10	-0.8830528998050653
1116	11	-0.7632596955579061
1116	12	-1.341087770180529
1116	13	-1.1174603819809121
1116	14	-1.2909374547109564
1116	15	-1.1675730768330723
1116	16	-1.3702830888190505
1116	17	-1.1153844923982916
1116	18	-1.8668021176489091
1116	19	-1.1899795291584638
1116	20	-1.1164274333297968
1116	21	-1.2138575991235285
1116	22	-2.693720151737921
1116	23	-2.2760710956175885
1116	24	-1.674518663721237
1116	25	-1.9272929176225375
1201	1	0.5780438511246864
1201	2	0.5807033055185506
1201	3	0.49921387693641606
1201	4	0.5214960725080644
1201	5	0.6118253696086882
1201	6	0.6446684876770377
1201	7	0.7006393350871889
1201	8	0.7378286138112742
1201	9	0.8470480764566659
1201	10	0.7835520455261715
1201	11	0.8537757560879626
1201	12	0.6850112780401716
1201	13	0.7506429395346004
1201	14	0.7489227283109585
1201	15	0.9320887265554276
1201	16	0.8916232290857593
1201	17	0.7722363861141517
1201	18	0.9391087894187322
1201	19	0.7415170498457542
1201	20	0.9077963754938168
1201	21	0.8745318915483793
1201	22	0.9062938263292182
1201	23	0.9157424769040716
1201	24	0.936763088729009
1201	25	0.8206344254736635
1202	1	0.8864802228170383
1202	2	1.0113428188483873
1202	3	0.9749869767694932
1202	4	0.9802948152383877
1202	5	1.0026959501002608
1202	6	1.0661499606213454
1202	7	0.9145989598015447
1202	8	0.9528315879494045
1202	9	1.189869838823988
1202	10	1.2023548732944391
1202	11	1.2799969615327171
1202	12	1.2815196590909679
1202	13	1.2452424297372318
1202	14	1.3455332353102634
1202	15	1.4693083524094916
1202	16	1.3866251403456786
1202	17	1.593911354115761
1202	18	1.5134487964747052
1202	19	1.5920021000795543
1202	20	1.72993395163207
1202	21	1.6337147430969452
1202	22	1.470872001341725
1202	23	1.6720558774292371
1202	24	1.3436421517570452
1202	25	1.4916035078832195
1203	1	0.46613366049171745
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2211	9	-0.28070048129716696
2211	10	-0.4635724456450916
2211	11	-0.14668256014519443
2211	12	-0.11427584163458704
2211	13	-0.09697794531202675
2211	14	-1.0505311377787478
2211	15	-0.6497661113916422
2211	16	-0.2693545164144453
2211	17	-0.17027896937091924
2211	18	-0.39548191905495145
2211	19	-0.24384393880603739
2211	20	-0.32381344924277755
2211	21	-0.34190400942758004
2211	22	-0.13196044902994686
2211	23	-0.36652484565284027
2211	24	-0.4118645788196176
2211	25	-0.5408632914558567
2212	1	-0.13937128529789788
2212	2	-0.11324472421334875
2212	3	-0.0626001624538084
2212	4	0.09065351734106031
2212	5	-0.124257987693845
2212	6	-0.15453081494544563
2212	7	-0.23121749562971416
2212	8	-0.40807315789093934
2212	9	-0.3603132428080116
2212	10	-0.46075337756802526
2212	11	-0.2835349503221565
2212	12	-0.4015225740370809
2212	13	-0.3065784753041001
2212	14	-0.447310575250107
2212	15	-0.25061522609152576
2212	16	-0.25052294770095074
2212	17	-0.5131806274751227
2212	18	-0.3530795226955519
2212	19	-0.16338200198322284
2212	20	-0.4824007270085673
2212	21	-0.38103274707180645
2212	22	-0.47990988157960857
2212	23	-0.8864662216441488
2212	24	-0.5463909802316351
2212	25	-0.6441302222295846
2213	1	-0.48777116570840917
2213	2	-0.5304036806119967
2213	3	-0.471019628694183
2213	4	-0.49856878487867107
2213	5	-0.41391990332223827
2213	6	-0.129604643151481
2213	7	-0.751674620356674
2213	8	-0.384676328232743
2213	9	-0.5582192064186344
2213	10	-0.30543774673346036
2213	11	-0.4437946303592568
2213	12	-0.6675615992607007
2213	13	-0.3643545378811268
2213	14	-0.4054438809232863
2213	15	-0.5532031792807857
2213	16	-0.7505857931986526
2213	17	-0.6043581568538627
2213	18	-0.6985107160278261
2213	19	-1.0890369985053638
2213	20	-0.67114528141569
2213	21	-1.4021219876785338
2213	22	-0.8009432934189675
2213	23	-0.7679444941861604
2213	24	-0.8975151824599052
2213	25	-0.8286156213289999
2214	1	-1.1480156147900118
2214	2	-1.129232886431243
2214	3	-0.9219330870927571
2214	4	-1.4532359527832952
2214	5	-1.4433369433952592
2214	6	-3.0142535402952184
2214	7	-1.2944583069725013
2214	8	-1.9367569171023966
2214	9	-1.3220969404619893
2214	10	-1.5985262380656025
2214	11	-1.4743808716957716
2214	12	-1.504687084503118
2214	13	-1.5509721214575194
2214	14	-1.7745183482119344
2214	15	-2.0713160820698207
2214	16	-1.621594858490738
2214	17	-1.8076188584701995
2214	18	-1.6800048405525416
2214	19	-3.5542105707338116
2214	20	-2.0607718856824775
2214	21	-2.533110122037918
2214	22	-2.232789213718945
2214	23	-2.256493808514932
2214	24	-1.423592990096033
2214	25	-1.1881055055079912
2215	1	-1.4496636361188848
2215	2	-1.2851159697079417
2215	3	-1.310019565737747
2215	4	-1.0892323456945832
2215	5	-1.6337118323073838
2215	6	-1.590711732045964
2215	7	-1.8510938472516614
2215	8	-2.4995529336218176
2215	9	-2.0147210321552294
2215	10	-1.3256137099669019
2215	11	-2.0755829078712154
2215	12	-2.778755253252136
2215	13	-2.166392752436657
2215	14	-2.2058908716057317
2215	15	-3.3952738163999
2215	16	-2.6919151183056798
2215	17	-2.359731488508359
2215	18	-2.9255064349903037
2215	19	-3.542834845395319
2215	20	-2.9946579356589496
2215	21	-2.2846846293305134
2215	22	-3.0967000297574216
2215	23	-3.109236045432695
2215	24	-2.1511241600474165
2215	25	-2.3325488245523758
2216	1	-1.3214709047854498
2216	2	-1.0388981381257878
2216	3	-1.3606524650829606
2216	4	-0.9951622477300148
2216	5	-1.1086239560574427
2216	6	-1.4688400630951506
2216	7	-2.7875662978886844
2216	8	-1.8743042152826455
2216	9	-1.869754723338417
2216	10	-1.849331731894722
2216	11	-2.3117425571276016
2216	12	-1.7505917082544187
2216	13	-2.218417713098553
2216	14	-2.19812349939113
2216	15	-2.2894600252217856
2216	16	-2.125216410118277
2216	17	-2.2810800241156883
2216	18	-2.1425106479744187
2216	19	-2.278969115229337
2216	20	-2.6520417613530753
2216	21	-2.4683710538774903
2216	22	-2.950933317407795
2216	23	-1.9475142419709428
2216	24	-2.1666376492104007
2216	25	-1.991366587610088
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32123.0
3	0.0
4	531.0
5	4658.0
6	5473.0
7	4275.0
8	3507.0
9	2940.0
10	2634.0
11	2293.0
12	2175.0
13	1952.0
14	1967.0
15	1882.0
16	1951.0
17	2093.0
18	2234.0
19	2672.0
20	3617.0
21	4507.0
22	5623.0
23	7021.0
24	8135.0
25	9325.0
26	10525.0
27	11642.0
28	12990.0
29	15583.0
30	19872.0
31	25248.0
32	31854.0
33	41030.0
34	53598.0
35	70491.0
36	96750.0
37	150514.0
38	493515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.92877961373147	19.19530734074209	13.698932479340067	27.17698056618637
2	13.96652719665272	20.328626220362622	39.39757670850767	26.307269874476987
3	17.143305439330543	23.968880753138073	30.185059274755925	28.702754532775455
4	11.71859196584893	16.803847575734324	36.442457258846424	35.03510319957032
5	13.05352114641814	37.922413702865	35.49627176837162	13.527793382345244
6	33.00609889616173	36.55743439826289	17.173483105682504	13.26298359989287
7	28.182523696165884	31.632005301662875	21.584656569091653	18.600814433079584
8	25.614196984379372	36.50388967437333	19.959232339901455	17.92268100134584
9	26.249902983232886	14.749862432404385	19.687122016134868	39.31311256822786
10	14.86847317892988	28.903876258505655	34.20081291306647	22.026837649497992
11	34.09584812699785	22.457719830295275	24.151367409684187	19.295064633022683
12	23.183916783775206	24.84996739558742	31.121609228269246	20.844506592368127
13	28.80102723515613	21.6302563642117	25.634154697221046	23.934561703411127
14	20.97329433763245	22.09949134147245	27.138479777881418	29.788734543013685
15	23.35552754400922	30.56376433339784	23.036997906107853	23.043710216485085
16	22.212128609289998	28.032675408006696	27.394685451248428	22.360510531454874
17	21.898764859440202	28.620628922482418	27.55803835335518	21.922567864722204
18	22.000869951036925	27.12277205800952	29.322928788667273	21.55342920228628
19	23.300830385519948	27.11061404594579	27.74669667654017	21.841858891994093
20	23.61488129795547	27.02879638201538	27.798524701915518	21.557797618113632
21	22.673640167364017	26.736488842398888	27.5263249651325	23.063546025104603
22	23.22292480609208	27.49970798313101	27.005458536219678	22.271908674557224
23	22.094926778242677	27.40062761506276	27.78634937238494	22.718096234309623
24	23.070519525801952	26.93924337517434	27.963563458856346	22.026673640167363
25	22.69752756568561	26.75769655560776	27.704026172255354	22.840749706451273
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	178.0
1	178.0
2	304.0
3	430.0
4	430.0
5	430.0
6	930.5
7	1431.0
8	1431.0
9	1431.0
10	1886.5
11	2342.0
12	2342.0
13	2342.0
14	3638.0
15	4934.0
16	4934.0
17	4934.0
18	8689.5
19	12445.0
20	12445.0
21	12445.0
22	19601.5
23	26758.0
24	26758.0
25	26758.0
26	38765.5
27	50773.0
28	50773.0
29	50773.0
30	65112.0
31	79451.0
32	79451.0
33	79451.0
34	90687.5
35	101924.0
36	101924.0
37	101924.0
38	111370.5
39	120817.0
40	120817.0
41	120817.0
42	129046.0
43	137275.0
44	137275.0
45	137275.0
46	146405.0
47	155535.0
48	155535.0
49	155535.0
50	150882.0
51	146229.0
52	146229.0
53	146229.0
54	130897.5
55	115566.0
56	115566.0
57	115566.0
58	100010.5
59	84455.0
60	84455.0
61	84455.0
62	69252.0
63	54049.0
64	54049.0
65	54049.0
66	42023.0
67	29997.0
68	29997.0
69	29997.0
70	22056.5
71	14116.0
72	14116.0
73	14116.0
74	9816.5
75	5517.0
76	5517.0
77	5517.0
78	3783.0
79	2049.0
80	2049.0
81	2049.0
82	1369.5
83	690.0
84	690.0
85	690.0
86	443.0
87	196.0
88	196.0
89	196.0
90	117.0
91	38.0
92	38.0
93	38.0
94	21.5
95	5.0
96	5.0
97	5.0
98	2.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0783647140864714
2	0.0
3	0.0
4	0.02580195258019526
5	0.07078103207810321
6	0.08080543933054393
7	0.03399581589958159
8	0.06127963737796374
9	0.04279986052998606
10	0.1044281729428173
11	0.002702231520223152
12	0.010111576011157601
13	0.0037482566248256623
14	0.005665969316596932
15	0.004707112970711297
16	0.01394700139470014
17	0.024930264993026502
18	7.845188284518829E-4
19	0.001917712691771269
20	0.003661087866108787
21	0.0
22	5.230125523012553E-4
23	0.0
24	0.0
25	0.002702231520223152
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1147200.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.96810625434938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.93961789858747	59.69007233620681
2	11.744094103386294	16.904004245871658
3	2.9381881588523067	6.34367512834662
4	1.0197151225312941	2.9354786514999636
5	0.4651515377841011	1.6738037647810098
6	0.23641768914661104	1.0208720023746627
7	0.14511392521596936	0.7310502072250015
8	0.09834778115208173	0.5662322851066014
9	0.07323877778531794	0.4743770527433201
>10	0.30746250582310386	3.7825657015707543
>50	0.015184756552100324	0.7722229111394371
>100	0.014071245859451865	2.0594059897542074
>500	0.0020621590894886643	1.0415740855059872
>1k	0.001334338234375018	2.0046656378739405
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3603	0.31406903765690375	No Hit
CCCATGTACTCTGCGTTGATACCAC	3022	0.26342398884239887	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2958	0.25784518828451886	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2719	0.23701185495118549	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2257	0.19673988842398882	No Hit
GTCCTACAGTGGACATTTCTAAATT	1657	0.14443863319386332	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1563	0.13624476987447698	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1417	0.1235181311018131	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1417	0.1235181311018131	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1247	0.1086994421199442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.615062761506276E-4	8.716875871687587E-5
10	0.0	0.0	0.0	2.615062761506276E-4	8.716875871687587E-5
11	0.0	0.0	0.0	2.615062761506276E-4	8.716875871687587E-5
12	0.0	0.0	0.0	2.615062761506276E-4	8.716875871687587E-5
13	0.0	0.0	0.0	2.615062761506276E-4	8.716875871687587E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	55	0.0030504914	12.09994	3
TAGGACC	310	0.0	11.3575945	4
CTACACT	170	1.0913936E-11	11.195085	4
TAGAACG	60	0.005801741	11.101792	4
TTGACCC	60	0.005801741	11.101792	4
ATTAGGC	60	0.005841503	11.091613	3
ACGGCCA	70	0.0014703262	10.875227	5
TAGGACT	175	2.0008883E-11	10.875226	4
CCTACAC	140	8.240022E-9	10.865253	3
GTAATAC	115	8.7226726E-7	10.747151	3
GTATAGG	135	5.6383215E-8	10.560673	1
GACGTGG	425	0.0	10.512353	7
CGTGGAA	415	0.0	10.30395	9
GTAGGAC	900	0.0	10.246537	3
GTCCTAG	140	9.604446E-8	10.183507	1
CTAGGAC	150	2.41198E-8	10.140902	3
ATGGAAC	95	1.6306332E-4	10.007469	3
CCCCTAA	95	1.6349503E-4	10.0048485	1
GTCCTAA	535	0.0	9.948747	1
TGTAGGA	915	0.0	9.870324	2
>>END_MODULE