FastQCFastQC Report
Thu 26 May 2016
SRR1512962_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512962_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751930
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT55240.3153094016313437No Hit
CTGTAGGACGTGGAATATGGCAAGA47200.2694171570781938No Hit
GTCCTAAAGTGTGTATTTCTCATTT46470.2652503239284675No Hit
CTTTAGGACGTGAAATATGGCGAGG41080.2344842545078856No Hit
GTCCTACAGTGTGCATTTCTCATTT32640.18630881370831026No Hit
GTATCAACGCAGAGTACTTTTTTTT32230.18396853755572426No Hit
CTGTAGGACCTGGAATATGGCGAGA21670.12369215665009446No Hit
GTACATGGGAAGCAGTGGTATCAAC21430.12232223890223924No Hit
CTGAAGGACCTGGAATATGGCGAGA20720.11826956556483421No Hit
GTCCTTCAGTGTGCATTTCTCATTT18940.10810934226824132No Hit
GGTATCAACGCAGAGTACTTTTTTT18270.10428498855547881No Hit
TATCAACGCAGAGTACTTTTTTTTT17940.10240135165217787No Hit
CCCATGTACTCTGCGTTGATACCAC17760.10137391334128645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGC551.9697694E-413.8103968
TAGGACC8750.013.5707554
GACCGTG751.4883035E-512.6588077
GGACCGT604.1139708E-412.6588066
CCCGTTT701.0886505E-412.2171712
GCGCCAC550.003063291312.09376413
AATGTCC7150.012.0840968
CCGTTTC658.008439E-411.69506713
GTCCTAA14550.011.3273251
GACGTGG11450.011.2768417
AAATGTC7300.011.1848367
TGTAGGA27800.011.0713932
GGACGTG24600.010.9606736
CCACCTT6850.010.95879113
AGGACGT24950.010.8889285
GGCGAGG10900.010.88804519
CTGTAGG28000.010.8536791
CGTGCGC700.001498237810.85133610
CTGCGTG700.001498237810.8513369
AGGACCT15600.010.8388965
Produced by FastQC (version 0.11.5)