##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512962_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1751930
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.118834085836763	31.0	30.0	34.0	23.0	34.0
2	29.24513251100215	31.0	30.0	34.0	22.0	34.0
3	29.366706432334624	31.0	30.0	34.0	23.0	34.0
4	32.78714389273544	35.0	33.0	37.0	25.0	37.0
5	32.925096322341645	37.0	35.0	37.0	26.0	37.0
6	32.88575856341292	37.0	35.0	37.0	26.0	37.0
7	32.776653176782176	37.0	35.0	37.0	25.0	37.0
8	32.75327610121409	37.0	35.0	37.0	25.0	37.0
9	34.068000433807285	39.0	35.0	39.0	25.0	39.0
10	33.94340413144361	38.0	34.0	39.0	24.0	39.0
11	34.020649797651735	39.0	35.0	39.0	25.0	39.0
12	33.9230677024767	39.0	34.0	39.0	23.0	39.0
13	33.9246807806248	39.0	35.0	39.0	23.0	39.0
14	34.918757027963444	39.0	35.0	41.0	23.0	41.0
15	34.8786732346612	39.0	35.0	41.0	22.0	41.0
16	34.78000433807287	39.0	34.0	41.0	21.0	41.0
17	34.71624151649895	39.0	34.0	41.0	19.0	41.0
18	34.41914631292346	39.0	34.0	41.0	17.0	41.0
19	34.657993184659205	39.0	34.0	41.0	18.0	41.0
20	34.74986158122756	39.0	34.0	41.0	19.0	41.0
21	34.679924997003305	39.0	34.0	41.0	18.0	41.0
22	34.591047016718704	39.0	34.0	41.0	18.0	41.0
23	34.32187815723231	39.0	34.0	41.0	16.0	41.0
24	34.33728002831163	39.0	34.0	41.0	16.0	41.0
25	34.246661110889136	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.196760391909109
1101	2	0.45849698514888004
1101	3	0.5906121622439748
1101	4	0.4992549857224944
1101	5	0.2653617365767502
1101	6	0.3675495932192021
1101	7	0.5848725881282348
1101	8	0.29088874001663356
1101	9	0.17100748544667255
1101	10	0.17990171607365113
1101	11	0.3341457094903717
1101	12	0.5980255372062686
1101	13	0.4848462945148242
1101	14	0.3700431113457583
1101	15	0.1521111901781893
1101	16	0.684916281368622
1101	17	0.5798469411347327
1101	18	0.7798601579308553
1101	19	0.32956069280514555
1101	20	0.5340894416001021
1101	21	0.518791044581441
1101	22	0.472784275488884
1101	23	0.714794238465899
1101	24	0.35636696111124166
1101	25	0.5390820456637684
1102	1	0.6251442892451031
1102	2	0.6756988459746722
1102	3	0.7132431036583284
1102	4	0.8386466450853547
1102	5	0.6815774654758755
1102	6	0.5661168956386433
1102	7	0.7579070180147838
1102	8	0.7459033510103623
1102	9	0.7345568011939392
1102	10	0.9815544428151952
1102	11	0.8926888534400064
1102	12	0.8430935557711408
1102	13	0.754247396287461
1102	14	0.9573736072112311
1102	15	1.07991735845102
1102	16	1.081535993568309
1102	17	0.9024762557161026
1102	18	1.3630457544938963
1102	19	1.23358568095977
1102	20	1.1528796336951856
1102	21	1.0211412081798343
1102	22	1.2634941101687645
1102	23	1.4335728393759766
1102	24	1.2492596667310636
1102	25	1.1505931091448218
1103	1	0.6082820227789796
1103	2	0.7235712489577395
1103	3	0.7582409017721545
1103	4	0.8186641033638651
1103	5	0.30145285309278336
1103	6	0.7338661236717243
1103	7	0.6048037959431269
1103	8	0.673031634074917
1103	9	0.9312689996433434
1103	10	0.8373030645128523
1103	11	0.8926665078889258
1103	12	0.9757423660338915
1103	13	0.9540913694571174
1103	14	1.1459567293943493
1103	15	1.1775291286760279
1103	16	1.2215425012200285
1103	17	0.9308543215322445
1103	18	1.3582739992704589
1103	19	1.4004416096526313
1103	20	1.2725164074157718
1103	21	1.0772322003092327
1103	22	1.24151693399304
1103	23	1.4782983092532547
1103	24	1.3575728651112087
1103	25	1.2216631996900418
1104	1	0.6029558135565587
1104	2	0.6310924181685671
1104	3	0.6462468891108486
1104	4	0.7901051118635181
1104	5	0.5298835517281262
1104	6	0.535456742052979
1104	7	0.7733326883539817
1104	8	0.7280446853872391
1104	9	0.8415312362477678
1104	10	1.0145375260672438
1104	11	0.9834353592135869
1104	12	0.9437771177696916
1104	13	0.8694234071746081
1104	14	1.0422762517064257
1104	15	1.1534936278885297
1104	16	0.9841202641791682
1104	17	1.0409098584376295
1104	18	1.1942755755269445
1104	19	1.201083226223929
1104	20	1.1193980103052041
1104	21	1.2273399272392922
1104	22	1.0474060992089278
1104	23	1.3902341130869686
1104	24	1.1690772537370933
1104	25	0.8802618285092976
1105	1	0.6215307769406202
1105	2	0.7236943415284074
1105	3	0.7079056451279371
1105	4	0.8496043182880371
1105	5	0.5569184665386402
1105	6	0.2921260648501587
1105	7	0.6762765998149547
1105	8	0.7090651516511244
1105	9	0.5850556000944636
1105	10	0.7956906984360543
1105	11	0.7156002461723858
1105	12	0.7036999493956202
1105	13	0.6957855276404246
1105	14	0.7880687451501984
1105	15	1.0182154948721305
1105	16	1.0068556618631632
1105	17	0.8861843562205891
1105	18	0.9408410581820092
1105	19	0.9218854981444125
1105	20	0.8952359090197675
1105	21	0.9100618735553283
1105	22	0.6070103709094852
1105	23	0.9128009293304373
1105	24	0.9392790342985364
1105	25	0.9765424173166934
1106	1	0.2247464159648409
1106	2	0.2730936465382747
1106	3	0.2690359645090794
1106	4	0.282737710992393
1106	5	0.29798312856039644
1106	6	0.16022811315022523
1106	7	-0.10742867547448043
1106	8	0.08972067752117141
1106	9	0.27284001345643816
1106	10	0.39930010699595897
1106	11	0.25607032492629145
1106	12	0.43456152761693545
1106	13	0.34767055741323816
1106	14	0.5945873335169765
1106	15	0.47473146392935206
1106	16	0.42552871964512917
1106	17	0.43336947155751915
1106	18	0.63965538405904
1106	19	0.24975145934370602
1106	20	0.4137142647918495
1106	21	0.18954220254060772
1106	22	0.3749063668582906
1106	23	0.521186212303185
1106	24	0.4653848194802279
1106	25	0.47455610757461386
1107	1	0.4601577459498145
1107	2	0.5023347470972048
1107	3	0.6165457616312828
1107	4	0.607599469962679
1107	5	0.5557195924984129
1107	6	0.4491628648756745
1107	7	0.5934695649586459
1107	8	0.5695093356044012
1107	9	0.7616230076657828
1107	10	0.46242414918976493
1107	11	0.4788805609148241
1107	12	0.7570033694386211
1107	13	0.6980477139919472
1107	14	0.8372382800438913
1107	15	0.9110085947801281
1107	16	0.9017706517010211
1107	17	0.9995286640733028
1107	18	1.3148159972041498
1107	19	1.2118784509280616
1107	20	1.084144667005198
1107	21	1.0255490036636914
1107	22	1.0148857099384117
1107	23	1.1454457885007443
1107	24	1.0689024617575598
1107	25	1.0390455945447172
1108	1	0.5990238174184626
1108	2	0.5757267922712082
1108	3	0.5065807583171456
1108	4	0.6461938271024366
1108	5	0.598985161834193
1108	6	0.7345414619858701
1108	7	0.7091149837443425
1108	8	0.7503998583702014
1108	9	0.7953104972857332
1108	10	0.795111113634988
1108	11	0.6630036762333162
1108	12	0.5781320013360229
1108	13	0.6767182887964296
1108	14	0.8263884096917238
1108	15	0.8953034766457364
1108	16	0.7908069186812909
1108	17	0.8950540557474156
1108	18	1.1222040534442286
1108	19	1.1416307210743497
1108	20	1.0272866482156147
1108	21	0.7989682801195528
1108	22	0.7525482185978092
1108	23	1.0031586002203667
1108	24	0.9836618165258031
1108	25	1.1787091535535623
1109	1	0.0764130383182291
1109	2	0.06135328689171615
1109	3	0.3536964804928786
1109	4	0.43373963418861905
1109	5	0.22005202684370317
1109	6	0.20008879220688414
1109	7	0.23795166538324963
1109	8	0.35552943914232316
1109	9	0.5573410016425129
1109	10	0.6146440223398741
1109	11	0.19679334035618012
1109	12	0.414771218044379
1109	13	0.4428909273404784
1109	14	0.256524887246826
1109	15	0.6351083040381837
1109	16	0.5809300649962239
1109	17	0.4983096929466555
1109	18	-1.214159545970297
1109	19	0.21765003511497838
1109	20	0.46080775694397147
1109	21	0.5453955433185698
1109	22	0.659088299448932
1109	23	0.2697163409003096
1109	24	0.5185829086917195
1109	25	0.6379879050097585
1110	1	0.34662880939194807
1110	2	0.36034267913901985
1110	3	0.45338887774145675
1110	4	0.18131836001489177
1110	5	0.48323863604991146
1110	6	0.45043891173854433
1110	7	0.4619250139216291
1110	8	0.24798139746446424
1110	9	0.6640269080721026
1110	10	0.6582990241072864
1110	11	0.5145818486198621
1110	12	-0.005233415536089581
1110	13	0.2530290795974963
1110	14	0.45097235561426174
1110	15	0.6237915577048909
1110	16	0.7344190673477797
1110	17	0.24159867738305252
1110	18	0.8159679891450651
1110	19	0.5795383790210451
1110	20	0.5988104249321964
1110	21	0.5641711869256909
1110	22	0.5659552352489783
1110	23	0.48926774128962336
1110	24	0.6567718334193842
1110	25	0.7198575115893391
1111	1	-0.05121909247866441
1111	2	0.21946881272253904
1111	3	0.27982195459344794
1111	4	0.05293479716815597
1111	5	0.00288106338271632
1111	6	0.3056927830477676
1111	7	0.14101757133547466
1111	8	-0.08549574735435783
1111	9	0.25223060665637576
1111	10	0.30988388846110837
1111	11	0.06430440681848637
1111	12	0.2859149086591728
1111	13	0.20287396957711223
1111	14	0.1416389770605022
1111	15	0.37214604013406216
1111	16	0.15840437806932073
1111	17	0.021577095143847203
1111	18	0.3030432933871978
1111	19	-0.04966526422077777
1111	20	-0.018602122501604867
1111	21	0.1904414176458502
1111	22	0.027297485420227474
1111	23	0.40754955948219873
1111	24	0.5025327006841422
1111	25	-0.048202036356208566
1112	1	-0.24088775134437057
1112	2	-0.016177222799598212
1112	3	0.003732374247366721
1112	4	0.13941084739752085
1112	5	0.11775482655654912
1112	6	0.07338383886876443
1112	7	-0.23297439348250748
1112	8	0.06802046570370379
1112	9	0.16322757737833626
1112	10	0.1738687172429607
1112	11	0.19607546526643915
1112	12	0.18899950232291474
1112	13	-0.13105020690215952
1112	14	0.2016074428821426
1112	15	0.06619229542368288
1112	16	0.1448209479825877
1112	17	0.2104417819631621
1112	18	0.20830994433838868
1112	19	0.0988417077639383
1112	20	0.02577545256492897
1112	21	0.04754916858777847
1112	22	0.11458004388540388
1112	23	0.295918781675617
1112	24	-4.40781186242134E-4
1112	25	0.14157728045842788
1113	1	-0.4565504164301615
1113	2	-0.2600442515310668
1113	3	-0.5431818212556045
1113	4	-0.25412372964286334
1113	5	-0.44502566281631317
1113	6	-0.2929596838308015
1113	7	-0.5343344100613194
1113	8	-0.14245243549164854
1113	9	-0.32619167029797325
1113	10	-0.5093093813732708
1113	11	-0.5158650684385364
1113	12	-0.1931432829540327
1113	13	-0.3980632846681331
1113	14	-0.2700671974159192
1113	15	-0.5912308910270596
1113	16	-0.5506349837118734
1113	17	-0.5484120325877768
1113	18	-0.657433824691374
1113	19	-0.5260577126309798
1113	20	-0.7150286511613544
1113	21	-0.6245195059513904
1113	22	-0.40910055684042135
1113	23	-0.9382341975844071
1113	24	-0.3656927260737177
1113	25	-0.3754454012061714
1114	1	-1.1252310254854692
1114	2	-1.0014069892026711
1114	3	-0.789575249140892
1114	4	-1.0303749587094266
1114	5	-0.999601055845492
1114	6	-1.4606159867636421
1114	7	-1.5549246764588496
1114	8	-1.0818883623049267
1114	9	-1.1505552533496868
1114	10	-1.3689292519310712
1114	11	-1.9377609371220217
1114	12	-1.3324291618543782
1114	13	-1.2313458898202612
1114	14	-1.4807254100128233
1114	15	-1.5409528804371604
1114	16	-2.211286833449492
1114	17	-4.3764906654344
1114	18	-3.1526829121552247
1114	19	-2.0912189684484233
1114	20	-1.2697933846072686
1114	21	-2.296122864519809
1114	22	-2.3862519121294454
1114	23	-2.1691956424197585
1114	24	-1.7734076828781653
1114	25	-1.5652699865150481
1115	1	-1.3356414942989367
1115	2	-0.8990601652601207
1115	3	-0.8656976565697647
1115	4	-0.8705327497391977
1115	5	-0.7469048947413199
1115	6	-0.807117818209818
1115	7	-1.8396088143970708
1115	8	-1.267240005324787
1115	9	-1.0415704228147078
1115	10	-0.9139722065899605
1115	11	-1.7082923726913393
1115	12	-1.2933288387158726
1115	13	-1.1573843472235268
1115	14	-1.1900359113702166
1115	15	-2.5189804462597607
1115	16	-1.2889868038864662
1115	17	-1.0869205969358688
1115	18	-1.2827349086435476
1115	19	-1.4616895704561372
1115	20	-1.157766297176103
1115	21	-1.6734910720959562
1115	22	-1.8790111410249821
1115	23	-1.226131746487404
1115	24	-1.2880963688631084
1115	25	-1.6366434980716207
1116	1	-0.6551281001272109
1116	2	-1.334620030281446
1116	3	-0.7733224466253397
1116	4	-0.37192264807319475
1116	5	-0.8523275822898455
1116	6	-0.4899906561387084
1116	7	-0.25600001293948793
1116	8	-1.0817226227784964
1116	9	-1.0373999684437578
1116	10	-1.6602160723652517
1116	11	-0.4312234424076493
1116	12	-0.35996464687767116
1116	13	-0.9000880348711107
1116	14	-0.563170416032996
1116	15	-0.268144507587742
1116	16	0.022429734798407708
1116	17	-0.604083769970913
1116	18	-0.22907469115690304
1116	19	-0.5901678811065594
1116	20	-0.9696862974865965
1116	21	-1.306468475131716
1116	22	-0.49018537350967506
1116	23	-0.4141162344356246
1116	24	-0.9137127866226891
1116	25	-0.5544058638468243
1201	1	0.5259166154616537
1201	2	0.4823104782297598
1201	3	0.6627482829801643
1201	4	0.6995554430660178
1201	5	0.6743914620714548
1201	6	0.6567880872477616
1201	7	0.5336100599578089
1201	8	0.1668108895814555
1201	9	0.5588287050393603
1201	10	0.847879639782164
1201	11	0.36471113695051827
1201	12	-0.21763517343771355
1201	13	0.29279584637632183
1201	14	0.8059957930505135
1201	15	0.5263527269589474
1201	16	0.9462435191433656
1201	17	1.1096199215223947
1201	18	1.3781167336111437
1201	19	0.8451174512407817
1201	20	0.8414366249925322
1201	21	0.7213299186449262
1201	22	0.9856666184637817
1201	23	1.2346637024265732
1201	24	1.1529376115890244
1201	25	0.960418647425179
1202	1	0.648595764545373
1202	2	0.691447176719759
1202	3	0.47172698117859113
1202	4	0.8284849943740795
1202	5	0.7772514906244581
1202	6	0.6993132812676492
1202	7	0.8186508602824816
1202	8	0.9407589661938687
1202	9	1.2142407075453008
1202	10	1.2691243090782223
1202	11	0.8478152188217223
1202	12	0.9244005217570006
1202	13	1.0481882794363386
1202	14	1.1316300598070157
1202	15	0.6807108688334864
1202	16	0.9070272688856136
1202	17	1.13215197141529
1202	18	1.479946673812698
1202	19	1.2627457131981785
1202	20	1.0738496690132777
1202	21	1.031545957447591
1202	22	1.19142226832043
1202	23	1.3148313167009746
1202	24	1.4088450231554859
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2211	10	0.20938465634564096
2211	11	0.12857374298686608
2211	12	0.14525954895376003
2211	13	0.08556058480855455
2211	14	0.41147496699922925
2211	15	0.32402116807016057
2211	16	0.23617934556953202
2211	17	0.3061087230911994
2211	18	0.2696693866715094
2211	19	0.10560307739513064
2211	20	-0.05016750843190465
2211	21	-0.07830731638960486
2211	22	-0.13847273544972438
2211	23	0.5414465439948302
2211	24	0.27936163765973987
2211	25	0.2918893595860581
2212	1	-0.12407338088691588
2212	2	-0.15409793062393007
2212	3	-0.04262358096552887
2212	4	-0.273141111247611
2212	5	-0.28832727335334596
2212	6	-0.21975148374865228
2212	7	-0.22911844016741156
2212	8	-0.19176440962717578
2212	9	-0.09676023620491492
2212	10	-0.021767680162767533
2212	11	-0.23368752967705575
2212	12	-0.13526811705703068
2212	13	-0.20975621491189855
2212	14	-0.2027000860004975
2212	15	-0.4355835408046431
2212	16	-0.08391209737411032
2212	17	-0.08695486439364686
2212	18	-2.8298297950959785
2212	19	-0.9933418546930923
2212	20	-0.524675705486267
2212	21	-0.6520540137471826
2212	22	-0.24822572674730736
2212	23	-0.2717526486417867
2212	24	-0.5915337403169616
2212	25	-0.3401492939008506
2213	1	-0.4943441525212364
2213	2	-0.3928291724069979
2213	3	-0.6409027183138853
2213	4	-0.7520970250513273
2213	5	-0.7541584035465903
2213	6	-0.6716961130745176
2213	7	-0.3983051181643944
2213	8	-0.847874702215833
2213	9	-0.6875715700984983
2213	10	-0.7217734462019436
2213	11	-0.83776323229241
2213	12	-0.9835192721564425
2213	13	-0.6490305601859774
2213	14	-0.6714588508366361
2213	15	-0.9281242168192492
2213	16	-1.345063708460252
2213	17	-0.9242732439435741
2213	18	-0.8213702850840718
2213	19	-0.8271892612930429
2213	20	-1.471881224603294
2213	21	-1.0306787586129929
2213	22	-0.9843044600554762
2213	23	-1.3665850773679367
2213	24	-1.023407204717671
2213	25	-1.1280610667178692
2214	1	-1.6363810053570056
2214	2	-3.683576823588325
2214	3	-1.8284713480148582
2214	4	-1.4969126955446868
2214	5	-1.5494770129391426
2214	6	-1.5641608630422787
2214	7	-1.6073222868799846
2214	8	-1.3703213364988436
2214	9	-1.505114131164376
2214	10	-1.5572669523339542
2214	11	-1.2678255550197264
2214	12	-1.9240465414030048
2214	13	-1.8353448960038463
2214	14	-3.0176326012151122
2214	15	-3.112167985313409
2214	16	-2.1986186488614052
2214	17	-2.665503838034642
2214	18	-1.9977496817164493
2214	19	-4.290700841072777
2214	20	-2.543571391291451
2214	21	-2.428965352267788
2214	22	-2.451245465637875
2214	23	-2.546931860087085
2214	24	-2.8461171952707005
2214	25	-2.51928458836381
2215	1	-0.9950851899398252
2215	2	-2.375099197316672
2215	3	-1.3417187889397368
2215	4	-1.0304583944411583
2215	5	-0.8270053955148882
2215	6	-1.2944069524437438
2215	7	-1.8487891161499874
2215	8	-1.7295999477355295
2215	9	-1.550015196613387
2215	10	-2.8598613049051806
2215	11	-1.8459639337289673
2215	12	-2.2112551958253626
2215	13	-1.6738498214130715
2215	14	-2.99840872842886
2215	15	-2.8261286264284564
2215	16	-2.3992991414841
2215	17	-2.483459066047274
2215	18	-1.3657866202414084
2215	19	-2.1789516403639624
2215	20	-2.369779731487533
2215	21	-2.1055581816008697
2215	22	-1.8241175755446477
2215	23	-2.18344039734626
2215	24	-2.1114917962091724
2215	25	-2.124553602265692
2216	1	-1.3096709468465164
2216	2	-1.425194953194577
2216	3	-1.859890643042597
2216	4	-2.747836107854102
2216	5	-1.769370512941137
2216	6	-1.7138803478733138
2216	7	-1.690564398471551
2216	8	-2.1158529120789957
2216	9	-2.1168992264849997
2216	10	-1.9126523381453957
2216	11	-2.459380572038178
2216	12	-2.7617703782759193
2216	13	-3.273384495328699
2216	14	-2.6392463681387923
2216	15	-2.4825793543577817
2216	16	-1.7500657160879527
2216	17	-1.859872652302741
2216	18	-1.9125929111124194
2216	19	-2.2787210876441506
2216	20	-2.15909635634344
2216	21	-2.4275155583646395
2216	22	-2.983808967275877
2216	23	-2.9428306724531588
2216	24	-2.891443206036417
2216	25	-2.77734358034472
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83742.0
3	6.0
4	635.0
5	4050.0
6	5266.0
7	4552.0
8	4112.0
9	3747.0
10	3580.0
11	3445.0
12	3181.0
13	3121.0
14	3126.0
15	3093.0
16	3275.0
17	3650.0
18	3904.0
19	4900.0
20	6143.0
21	7994.0
22	9891.0
23	11865.0
24	13880.0
25	15692.0
26	17613.0
27	19467.0
28	22208.0
29	26686.0
30	32153.0
31	40600.0
32	51216.0
33	65373.0
34	85937.0
35	112662.0
36	155211.0
37	243101.0
38	672853.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.361150891214336	19.213350686369083	12.873401508156004	25.552096914260574
2	14.185953551584923	19.953802842638435	42.68668733832423	23.17355626745242
3	18.298481502862778	24.65894770141229	29.95612900573355	27.086441789991383
4	11.979354328847238	16.884842539355883	38.74483729742477	32.39096583437211
5	12.546704515669246	38.341359400491896	36.5814493061492	12.530486777689646
6	31.688503719435264	37.703370042418115	18.104829271356067	12.503296966790552
7	27.851750696642018	31.68957597465656	22.518678455392934	17.93999487330849
8	24.468238774849542	38.17478936517516	20.563851027496003	16.7931208324793
9	26.38126231052496	15.018355171134138	20.69395678113671	37.90642573720419
10	14.717095963113772	28.96900341869021	35.39235008118533	20.921550537010692
11	33.58190890510282	22.175635711757387	25.91089188047363	18.331563502666164
12	23.673995566235565	24.63274146060738	32.3012242236773	19.39203874947975
13	28.716596377305148	22.580326552936253	25.936688763046163	22.76638830671244
14	19.955727024044098	23.277342159760693	27.788629370557498	28.978301445637705
15	22.126400521639532	33.04097690934286	23.063821477776933	21.768801091240668
16	21.10846834831639	28.331165709600842	29.397145587810456	21.163220354272312
17	21.00063145370234	29.641156165465993	28.634655503752754	20.723556877078916
18	20.94953187302781	27.475599520760476	31.362478881865858	20.212389724345858
19	23.19962865521116	27.24873706231102	27.939470342660343	21.61216393981748
20	23.315002546365548	27.905254647973525	28.689032815001404	20.09070999065952
21	21.91516305880714	27.288812609409096	27.59570048656716	23.2003238452166
22	22.780168341075942	29.332544704358337	27.345233942484516	20.54205301208121
23	21.053593154710416	28.99313792491169	28.649720155911098	21.303548764466797
24	22.797159839254714	27.31716479474946	29.239074646992364	20.646600719003466
25	21.788622992095412	28.109607967959167	28.843601111038158	21.25816792890726
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	177.0
1	177.0
2	345.5
3	514.0
4	514.0
5	514.0
6	1067.0
7	1620.0
8	1620.0
9	1620.0
10	2424.5
11	3229.0
12	3229.0
13	3229.0
14	5706.5
15	8184.0
16	8184.0
17	8184.0
18	14824.5
19	21465.0
20	21465.0
21	21465.0
22	34851.0
23	48237.0
24	48237.0
25	48237.0
26	70298.5
27	92360.0
28	92360.0
29	92360.0
30	119821.0
31	147282.0
32	147282.0
33	147282.0
34	166504.5
35	185727.0
36	185727.0
37	185727.0
38	196278.0
39	206829.0
40	206829.0
41	206829.0
42	214730.5
43	222632.0
44	222632.0
45	222632.0
46	226721.0
47	230810.0
48	230810.0
49	230810.0
50	217030.5
51	203251.0
52	203251.0
53	203251.0
54	176910.0
55	150569.0
56	150569.0
57	150569.0
58	127733.0
59	104897.0
60	104897.0
61	104897.0
62	84949.0
63	65001.0
64	65001.0
65	65001.0
66	49872.5
67	34744.0
68	34744.0
69	34744.0
70	24992.5
71	15241.0
72	15241.0
73	15241.0
74	10549.0
75	5857.0
76	5857.0
77	5857.0
78	4077.0
79	2297.0
80	2297.0
81	2297.0
82	1502.0
83	707.0
84	707.0
85	707.0
86	458.0
87	209.0
88	209.0
89	209.0
90	132.0
91	55.0
92	55.0
93	55.0
94	34.5
95	14.0
96	14.0
97	14.0
98	18.0
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3551511761314664
2	0.04223913055886936
3	0.037615658159857984
4	0.03807229740914306
5	0.04343780858824268
6	0.018265569971402967
7	0.017866010628278528
8	0.02505807880451845
9	0.021690364341041025
10	0.021804524153362294
11	0.019977967156221996
12	0.021176645185595314
13	0.020605846123988972
14	0.029852790922011724
15	0.030195270358975526
16	0.02260364283961117
17	0.023745240962823856
18	0.09458140450817099
19	0.026142597021570495
20	0.023631081150502588
21	0.02717003533246191
22	0.020948325560952778
23	0.02391648068130576
24	0.019350088188455017
25	0.023688161056663222
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1751930.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.51242649550522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.47377118370709	60.409243525729806
2	10.84164296163819	15.506243907693252
3	2.5678243250357857	5.508940448924758
4	0.862426039706852	2.466967150893837
5	0.39690983250737577	1.4191992611263502
6	0.19676517726862913	0.8442693165778737
7	0.12491582164459042	0.6253123459439089
8	0.09154229859971892	0.5237129519873592
9	0.06733479204612584	0.4333746930109834
>10	0.33179823426586824	4.22619753453494
>50	0.022841924722517046	1.1561809629970368
>100	0.018150259452021252	2.7628623190348884
>500	0.002238434967603619	1.1456290294434968
>1k	0.0017587703316885575	2.6560596376523486
>5k	7.994410598584353E-5	0.31580691444911807
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	5524	0.3153094016313437	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4720	0.2694171570781938	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4647	0.2652503239284675	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4108	0.2344842545078856	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3264	0.18630881370831026	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3223	0.18396853755572426	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2167	0.12369215665009446	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2143	0.12232223890223924	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2072	0.11826956556483421	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1894	0.10810934226824132	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1827	0.10428498855547881	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1794	0.10240135165217787	No Hit
CCCATGTACTCTGCGTTGATACCAC	1776	0.10137391334128645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTGC	55	1.9697694E-4	13.810396	8
TAGGACC	875	0.0	13.570755	4
GACCGTG	75	1.4883035E-5	12.658807	7
GGACCGT	60	4.1139708E-4	12.658806	6
CCCGTTT	70	1.0886505E-4	12.21717	12
GCGCCAC	55	0.0030632913	12.093764	13
AATGTCC	715	0.0	12.084096	8
CCGTTTC	65	8.008439E-4	11.695067	13
GTCCTAA	1455	0.0	11.327325	1
GACGTGG	1145	0.0	11.276841	7
AAATGTC	730	0.0	11.184836	7
TGTAGGA	2780	0.0	11.071393	2
GGACGTG	2460	0.0	10.960673	6
CCACCTT	685	0.0	10.958791	13
AGGACGT	2495	0.0	10.888928	5
GGCGAGG	1090	0.0	10.888045	19
CTGTAGG	2800	0.0	10.853679	1
CGTGCGC	70	0.0014982378	10.851336	10
CTGCGTG	70	0.0014982378	10.851336	9
AGGACCT	1560	0.0	10.838896	5
>>END_MODULE