FastQCFastQC Report
Thu 26 May 2016
SRR1512962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512962_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751930
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT50620.2889384849851307No Hit
CTGTAGGACGTGGAATATGGCAAGA48090.2744972687264902No Hit
CTTTAGGACGTGAAATATGGCGAGG43050.24572899602153053No Hit
GTCCTAAAGTGTGTATTTCTCATTT42470.24241836146421375No Hit
GTATCAACGCAGAGTACTTTTTTTT39110.22323951299424066No Hit
GTCCTACAGTGTGCATTTCTCATTT29870.17049767970181456No Hit
TATCAACGCAGAGTACTTTTTTTTT28550.16296313208861082No Hit
GGTATCAACGCAGAGTACTTTTTTT24250.13841877243953812No Hit
CTGAAGGACCTGGAATATGGCGAGA22530.12860102857990902No Hit
CTGTAGGACCTGGAATATGGCGAGA22000.1255757935533954No Hit
GTACATGGGAAGCAGTGGTATCAAC20680.11804124594019168No Hit
CCCATGTACTCTGCGTTGATACCAC20650.11787000622170977No Hit
ATTTAGAAATGTCCACTGTAGGACG17960.10251551146449915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8400.012.5732154
ATAGGCG550.00303298512.1098275
ACATCGG550.003057773712.0966758
TCGAACT907.543793E-611.59973919
GCCTTAC1001.90828E-611.4106541
TGTCGAG1251.8586434E-811.38883518
GGCGAGG11800.011.34057619
TGTAGGA28300.010.950562
AGGACGT27150.010.9342095
TAGGGGT1408.1490725E-910.8741314
ATGTCGA1408.463758E-910.84774817
TATTGCG700.001502376310.84774816
CCACCTT6400.010.82656113
GGACGTG26650.010.8146226
GTATTAG1951.8189894E-1210.7279651
TTAGGAC15900.010.702213
GTCCACG803.7387692E-410.697491
AATGTCC7250.010.61885458
GTCCTAG2600.010.6060571
GTAGGAC27800.010.5323073
Produced by FastQC (version 0.11.5)