##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512962_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1751930
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.375169099222	31.0	31.0	34.0	26.0	34.0
2	30.553001546865456	31.0	31.0	34.0	27.0	34.0
3	30.641036456936064	31.0	31.0	34.0	27.0	34.0
4	34.101491497947976	37.0	35.0	37.0	30.0	37.0
5	33.75688925927406	37.0	35.0	37.0	28.0	37.0
6	33.61817880851404	37.0	35.0	37.0	28.0	37.0
7	33.47878739447352	37.0	35.0	37.0	28.0	37.0
8	33.358040560981316	37.0	35.0	37.0	27.0	37.0
9	34.802239244718685	39.0	35.0	39.0	27.0	39.0
10	34.6812304144572	39.0	35.0	39.0	27.0	39.0
11	34.71534707436941	39.0	35.0	39.0	27.0	39.0
12	34.57566626520466	39.0	35.0	39.0	27.0	39.0
13	34.553910829770594	39.0	35.0	39.0	27.0	39.0
14	35.60248525911423	40.0	36.0	41.0	27.0	41.0
15	35.62053506704035	40.0	36.0	41.0	27.0	41.0
16	35.602469847539574	40.0	36.0	41.0	27.0	41.0
17	35.58233376904328	40.0	36.0	41.0	27.0	41.0
18	35.51205470538206	40.0	36.0	41.0	27.0	41.0
19	35.49314641566729	40.0	36.0	41.0	26.0	41.0
20	35.47916583425137	40.0	36.0	41.0	26.0	41.0
21	35.41783575827802	40.0	36.0	41.0	26.0	41.0
22	35.38427562745087	40.0	36.0	41.0	25.0	41.0
23	35.268365174407656	40.0	36.0	41.0	25.0	41.0
24	35.25932771286524	40.0	36.0	41.0	25.0	41.0
25	35.149519672589655	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6565673644258112
1101	2	0.6437098021129337
1101	3	0.5884090158135571
1101	4	0.39259232037520775
1101	5	0.6491058273792234
1101	6	0.6433769079821801
1101	7	0.6765404656137264
1101	8	0.7435163036897237
1101	9	0.6489354958397371
1101	10	0.9076738891950455
1101	11	0.9505640724874027
1101	12	0.9616954647766605
1101	13	0.9735117383961551
1101	14	0.9941824100897492
1101	15	0.9412008545752215
1101	16	1.094745695438725
1101	17	1.142625683420782
1101	18	0.41270099343982025
1101	19	0.8322864054773333
1101	20	0.9070287912259474
1101	21	1.027803797864891
1101	22	0.9049723100998577
1101	23	0.8684264305746794
1101	24	0.825554254583345
1101	25	0.9125576246902725
1102	1	0.7400137287591235
1102	2	0.6962260043526776
1102	3	0.6031260248268886
1102	4	0.5285223502886041
1102	5	0.7678104073885592
1102	6	0.7397685246406809
1102	7	0.725668381457659
1102	8	0.6724447155821451
1102	9	0.9321892160339544
1102	10	0.99626714637904
1102	11	0.9001658712224057
1102	12	0.8446758770296938
1102	13	0.8521509468334543
1102	14	1.103194319185036
1102	15	1.0363498419733688
1102	16	0.9753821125017623
1102	17	1.0203935872261454
1102	18	1.11393514961172
1102	19	1.1237134701486866
1102	20	1.0752122762953462
1102	21	1.1597019933681878
1102	22	1.099265379994705
1102	23	1.206866392642354
1102	24	1.1287857549503997
1102	25	1.220995532881311
1103	1	0.862163527583828
1103	2	0.7340357221845579
1103	3	0.8418264284842429
1103	4	0.8421486934718345
1103	5	0.8812487081858151
1103	6	1.0273705333881153
1103	7	0.9775905562155458
1103	8	0.8680087970035828
1103	9	1.1492890509904967
1103	10	1.2102636939260591
1103	11	1.2210980335269
1103	12	1.285028806061007
1103	13	1.2379948442111797
1103	14	1.4399416432726255
1103	15	1.1642594352712692
1103	16	1.310845547470329
1103	17	1.4705028974804009
1103	18	1.5451585489319442
1103	19	1.5521648450534613
1103	20	1.4837674861409766
1103	21	1.285044853833945
1103	22	1.4613617063076987
1103	23	1.516877983580251
1103	24	1.5485345177778527
1103	25	1.4420556267326248
1104	1	0.7079947580154204
1104	2	0.716144246885122
1104	3	0.6259267405129911
1104	4	0.7311592525125619
1104	5	0.6799415723773095
1104	6	0.7117935567810108
1104	7	0.8368246610061547
1104	8	0.7554579435743776
1104	9	0.8004579344898701
1104	10	0.9785995380542829
1104	11	1.0013395367254674
1104	12	0.9198645818052853
1104	13	0.9928808713775013
1104	14	1.1749804648052162
1104	15	1.1196652312363895
1104	16	1.0347235748558816
1104	17	1.042252255445149
1104	18	1.1250027147149453
1104	19	1.1307409261986976
1104	20	1.2138111072375466
1104	21	1.0108109888312242
1104	22	1.2013774587227743
1104	23	1.1267452425864448
1104	24	1.270732761211029
1104	25	1.4091228533611044
1105	1	0.6127999692531141
1105	2	0.6417628014379737
1105	3	0.5554294466028615
1105	4	0.49869009048910584
1105	5	0.4885690006153425
1105	6	0.6259075263705753
1105	7	0.6710483503764593
1105	8	0.7329929289305142
1105	9	0.8960001792666077
1105	10	0.931399370753212
1105	11	0.9547057428120738
1105	12	0.7623416067784348
1105	13	0.8800408904373711
1105	14	1.0803073621686181
1105	15	1.0233442230515948
1105	16	1.1587933643085861
1105	17	0.8951760576382455
1105	18	1.2059310950929216
1105	19	1.2220164161392901
1105	20	1.1121264978820378
1105	21	1.087667054173039
1105	22	1.2683990627544688
1105	23	1.2585567235976214
1105	24	1.2411008386394968
1105	25	1.2643933644377725
1106	1	0.3459682860847977
1106	2	0.3239584813234231
1106	3	0.44360850260369844
1106	4	0.30689726041244825
1106	5	0.36181973838753123
1106	6	0.3700926591695648
1106	7	0.39992623816523576
1106	8	0.464146123615059
1106	9	0.4344961717229978
1106	10	0.4196552598341796
1106	11	0.3158867021778491
1106	12	0.4611969685050852
1106	13	0.40933088292868547
1106	14	0.6160181554701722
1106	15	0.5397349891123255
1106	16	0.5955588662556011
1106	17	0.4677527597528979
1106	18	0.6477405141300636
1106	19	0.6814956974007487
1106	20	0.7098459523671679
1106	21	0.5744884347237971
1106	22	0.6892295585024542
1106	23	0.755564598988336
1106	24	0.4944177576329807
1106	25	0.6307579374030041
1107	1	0.543300411323898
1107	2	0.5623430654444412
1107	3	0.5922700908001701
1107	4	0.6599542235469258
1107	5	0.6658396346554909
1107	6	0.6605218682034248
1107	7	0.7545925021338249
1107	8	0.7977363634068482
1107	9	0.6270663922001916
1107	10	0.8135625870748413
1107	11	0.8092552343060717
1107	12	0.6176999831476309
1107	13	0.8587165819267852
1107	14	0.8896500073965967
1107	15	0.8500671777390849
1107	16	0.8279920948128634
1107	17	0.8046469881199982
1107	18	0.8731664232768992
1107	19	1.0739361596086567
1107	20	0.996921954171853
1107	21	0.9442253013598858
1107	22	0.9827625256788295
1107	23	0.9349975404367825
1107	24	0.9909012339614165
1107	25	1.0211353177028712
1108	1	0.5971764551273608
1108	2	0.5137651518095012
1108	3	0.46140582677045217
1108	4	0.6023937274588036
1108	5	0.592030142907916
1108	6	0.534737285597636
1108	7	0.6785431626420362
1108	8	0.8593381612699389
1108	9	0.7605602703985568
1108	10	0.8475650319053116
1108	11	0.7781868852848248
1108	12	0.9054762796633753
1108	13	0.8307499058339047
1108	14	0.9423799434030897
1108	15	0.9735041844526435
1108	16	0.8110802717982395
1108	17	0.9007614432923674
1108	18	0.9261282740464836
1108	19	0.9054029531551393
1108	20	0.906178196045488
1108	21	0.898288971687812
1108	22	1.012605467016364
1108	23	0.9297712290336051
1108	24	0.8910701576824067
1108	25	0.959960691871153
1109	1	0.1809288029548597
1109	2	0.17473174800958802
1109	3	0.1961596179688847
1109	4	0.20988752035412972
1109	5	0.37406970072125034
1109	6	0.07185544133435684
1109	7	0.22624832478883405
1109	8	0.39382715664617507
1109	9	0.505541876437043
1109	10	0.4377767313894907
1109	11	0.40815580784728667
1109	12	0.5148435080812703
1109	13	0.43918562400620687
1109	14	0.5790724037840533
1109	15	0.5887401560404371
1109	16	0.4465244525645744
1109	17	0.5529184470961397
1109	18	0.5120408517972308
1109	19	0.7838460736204738
1109	20	0.708247129327809
1109	21	0.7948344327917383
1109	22	0.7898180388229292
1109	23	0.7369831556162723
1109	24	0.37537191111644574
1109	25	0.39078913395011483
1110	1	0.3607943225439172
1110	2	0.30307799370557476
1110	3	0.3503588827369022
1110	4	0.44795370341304164
1110	5	0.4213400879043476
1110	6	0.33422267314051624
1110	7	0.31682771773903795
1110	8	0.5258434721275336
1110	9	0.731764262416327
1110	10	0.5031413010438044
1110	11	0.45255697847343157
1110	12	0.763667413499391
1110	13	0.3521682008808824
1110	14	0.5880455886499547
1110	15	0.6024972716695913
1110	16	0.5958094829784741
1110	17	0.45177126798490974
1110	18	0.5499966257180944
1110	19	0.580932931938726
1110	20	0.7139310049499272
1110	21	0.7008134771322574
1110	22	0.7577721100425308
1110	23	0.780852200380572
1110	24	0.6527633880182009
1110	25	0.7177915144330527
1111	1	-0.4911671333488741
1111	2	-0.3696296755348456
1111	3	-0.2533831604720085
1111	4	-0.1937159242716433
1111	5	0.009391824809256377
1111	6	-0.10093274119759599
1111	7	0.07548627995807777
1111	8	0.16980514465774377
1111	9	-0.2711051316469977
1111	10	-0.2722640692241072
1111	11	-0.10276515728224211
1111	12	-0.12138777317225902
1111	13	-0.03631999205155978
1111	14	0.1880014724392325
1111	15	0.3818669225297455
1111	16	0.11220156041162
1111	17	0.27059771631051177
1111	18	0.23113277851897607
1111	19	0.250614250156346
1111	20	0.12284121755035926
1111	21	0.06900545258282165
1111	22	-0.057987236290884425
1111	23	-0.06898981074024135
1111	24	0.1308457420465885
1111	25	0.12597465086690107
1112	1	0.047404036264264704
1112	2	-0.1755912957807908
1112	3	-0.008434782605416302
1112	4	-0.25385978761428163
1112	5	0.00974809268105048
1112	6	-0.0681302011638465
1112	7	-0.05264614757297892
1112	8	0.012276546452348214
1112	9	-0.04578619247896398
1112	10	0.1911866380657301
1112	11	-0.010724307012722534
1112	12	0.09309189286231856
1112	13	0.0867089549466229
1112	14	0.14439903694988487
1112	15	0.014081146708065262
1112	16	-0.14572249646629842
1112	17	-0.07881163187011708
1112	18	0.010964039162630002
1112	19	0.02505210377123035
1112	20	0.23193700728904787
1112	21	0.30990980967615656
1112	22	0.22269671793276302
1112	23	0.31164216494865116
1112	24	0.15031807048471535
1112	25	0.2615716177979266
1113	1	-0.4946474505629759
1113	2	-0.9748061803340917
1113	3	-0.5051039937607804
1113	4	-0.6075376626622031
1113	5	-0.4410130456853878
1113	6	-0.5814851082072536
1113	7	-0.23237219995773728
1113	8	-0.19416729060176863
1113	9	-0.2781912621875904
1113	10	-0.49746542144133343
1113	11	-0.26229928105606604
1113	12	-0.473117906981976
1113	13	-0.3301888368361503
1113	14	-0.3229350331614498
1113	15	-0.3266164386758206
1113	16	-0.5965057172663251
1113	17	-0.38667172384280235
1113	18	-0.19897326501909873
1113	19	-0.43082693257750293
1113	20	-0.2682524573778835
1113	21	-0.491106034535278
1113	22	-0.6629239853025908
1113	23	-0.5588754603613211
1113	24	-0.8746842096245473
1113	25	-0.5829792869154602
1114	1	-0.6729316770012126
1114	2	-0.5429624817215704
1114	3	-0.4164382551174768
1114	4	-0.29220860070891774
1114	5	-0.41151195432400556
1114	6	-0.8181686918824838
1114	7	-0.7546695536398573
1114	8	-1.017811138187163
1114	9	-0.7488187046151396
1114	10	-0.887903905089324
1114	11	-0.6898554741069631
1114	12	-0.8535071043385258
1114	13	-0.8383537895621913
1114	14	-1.8516164780559308
1114	15	-1.1909241306940785
1114	16	-1.120982702999143
1114	17	-1.2027693681335734
1114	18	-1.337444671055124
1114	19	-1.3315472458747522
1114	20	-0.6335690019004971
1114	21	-0.7035313347688401
1114	22	-0.7603664383803661
1114	23	-0.5935269066923183
1114	24	-1.4734096489541173
1114	25	-1.3022144591352784
1115	1	-1.9916920151976143
1115	2	-1.836640986188364
1115	3	-1.5456833821017497
1115	4	-2.680962119005798
1115	5	-2.0222916209230917
1115	6	-1.7324317165809937
1115	7	-1.6711793973465134
1115	8	-1.659735302004247
1115	9	-2.016706904568281
1115	10	-1.818833502396906
1115	11	-1.9306950235623788
1115	12	-2.360726169842984
1115	13	-1.9743458852508908
1115	14	-2.7701158570587197
1115	15	-2.5299450547477775
1115	16	-2.3497335355897846
1115	17	-2.3864289872813274
1115	18	-2.195520868998038
1115	19	-2.0511415711048073
1115	20	-2.177513363791313
1115	21	-2.3277664475127935
1115	22	-2.56455631375983
1115	23	-2.83512079809978
1115	24	-2.1804430881673653
1115	25	-2.040096041774504
1116	1	-1.4084625607265941
1116	2	-1.5220955098589926
1116	3	-1.3532938791458626
1116	4	-1.657880846107659
1116	5	-1.6904021573700163
1116	6	-2.154933135335348
1116	7	-1.4397843167411821
1116	8	-1.6552826998746042
1116	9	-1.8828110869531827
1116	10	-1.989930805742702
1116	11	-1.845844653842505
1116	12	-2.632022208485079
1116	13	-1.9565159018878262
1116	14	-2.6156369217007622
1116	15	-2.3658538582472204
1116	16	-2.6986723512070654
1116	17	-2.541313088170746
1116	18	-3.3797339931550354
1116	19	-2.7221444595756097
1116	20	-2.5090530113732328
1116	21	-2.4954459155090163
1116	22	-4.056765441273576
1116	23	-3.9105441927051885
1116	24	-3.1293868312455047
1116	25	-3.50357908498108
1201	1	0.7265681918587603
1201	2	0.7028814479830459
1201	3	0.6460552774945931
1201	4	0.7470560358100116
1201	5	0.7596825264054488
1201	6	0.8422756205427078
1201	7	0.8809885494950507
1201	8	0.9072529202335176
1201	9	1.097435675116877
1201	10	0.8407675794685474
1201	11	1.00721822983526
1201	12	0.8695896778138774
1201	13	0.8583597020075828
1201	14	1.0023473662527849
1201	15	1.1589347399109542
1201	16	1.1454033415917095
1201	17	1.0339898513350505
1201	18	1.0931508724022763
1201	19	1.013842177786806
1201	20	1.244413013451883
1201	21	1.0966330400246278
1201	22	1.1151565830858843
1201	23	1.1633509786081078
1201	24	1.2631815029693385
1201	25	1.1450554137867144
1202	1	0.7662447837806496
1202	2	0.8028001494124055
1202	3	0.8165390368245582
1202	4	0.7812216616185665
1202	5	0.749229421496139
1202	6	0.9874505700758647
1202	7	0.8256814865011322
1202	8	0.8018032892292126
1202	9	1.043559151753719
1202	10	0.9463094101442451
1202	11	1.0220879879490283
1202	12	0.9370631572745012
1202	13	1.0299942498286114
1202	14	1.0040081423848903
1202	15	1.2107596235778217
1202	16	1.0114432606346213
1202	17	1.1840297125889947
1202	18	1.2554892767175332
1202	19	1.3703163152536249
1202	20	1.5170769929315426
1202	21	1.447714810100706
1202	22	1.3470437474994412
1202	23	1.4457221912964684
1202	24	1.3315856474890424
1202	25	1.3829568336879277
1203	1	0.5702483538431267
1203	2	0.48519415191350745
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2211	13	0.06710703426871589
2211	14	-1.1590136108920461
2211	15	-0.4266005449945851
2211	16	-0.1796674373088365
2211	17	-0.013904707375267833
2211	18	-0.27129948099062773
2211	19	-0.20251197226973972
2211	20	-0.3731967822845945
2211	21	-0.25481997345108454
2211	22	-0.11225304115497892
2211	23	-0.12503225283810337
2211	24	-0.087376940179972
2211	25	-0.26424318077294373
2212	1	-0.27240121892552693
2212	2	-0.1419008889655089
2212	3	-0.11862822931932016
2212	4	0.04194030150647876
2212	5	-0.16413291920652995
2212	6	-0.12685526816256498
2212	7	-0.1780216276218951
2212	8	-0.45602093066079874
2212	9	-0.48419392680496287
2212	10	-0.43584726755178593
2212	11	-0.17367271641458615
2212	12	-0.12530154975233643
2212	13	-0.22400089623789654
2212	14	-0.24449948152496148
2212	15	-0.18186699624244795
2212	16	-0.11231435601075646
2212	17	-0.34540531330412705
2212	18	0.00757102292073597
2212	19	0.031101156629048887
2212	20	-0.19927831196799417
2212	21	-0.10397162225817169
2212	22	-0.08975139300816437
2212	23	-0.39418732491468234
2212	24	-0.2829244804541773
2212	25	-0.6002628579698026
2213	1	-0.5258303815687917
2213	2	-0.5863813675311036
2213	3	-0.48223792117018505
2213	4	-0.5956148248284805
2213	5	-0.43666606545232867
2213	6	-0.32626394910413836
2213	7	-0.8369600189282735
2213	8	-0.5344932016218493
2213	9	-0.8828490046895396
2213	10	-0.6776672538959332
2213	11	-0.5986119316644789
2213	12	-0.7123397806793363
2213	13	-0.6805321357771632
2213	14	-0.6507517726123595
2213	15	-0.8990913032545578
2213	16	-1.0492538467781785
2213	17	-0.9063002965108637
2213	18	-1.1432675183287486
2213	19	-1.259563051948156
2213	20	-0.780406352738332
2213	21	-1.5442704178865228
2213	22	-1.0606091526488655
2213	23	-1.0465179918680008
2213	24	-1.1270569615964874
2213	25	-1.325756213066235
2214	1	-1.1611361980726436
2214	2	-1.0708506756755511
2214	3	-0.9733093142373725
2214	4	-1.546060843325911
2214	5	-1.3021785507322718
2214	6	-2.8751777181038776
2214	7	-1.4870415650727118
2214	8	-2.054524127237336
2214	9	-1.4860425119008767
2214	10	-1.6195056238137795
2214	11	-1.4576098144754894
2214	12	-1.5353011412700823
2214	13	-1.8390273353195283
2214	14	-1.9587948185241757
2214	15	-2.3481532243578798
2214	16	-1.732123418960576
2214	17	-1.8592148539888242
2214	18	-1.8705185299156284
2214	19	-3.552047570678205
2214	20	-2.2635830224176487
2214	21	-2.484187582227996
2214	22	-2.1336998906997025
2214	23	-2.3294734559141332
2214	24	-1.580358941369866
2214	25	-1.499331626633861
2215	1	-1.6513572194598858
2215	2	-1.3792173298432537
2215	3	-1.409971985908296
2215	4	-1.2757970451129097
2215	5	-1.9269109035394543
2215	6	-1.8332667667925975
2215	7	-1.9876145315673135
2215	8	-2.8205981444625436
2215	9	-2.349935226407034
2215	10	-1.9185184977459642
2215	11	-2.4761272622105466
2215	12	-3.1212738584862905
2215	13	-2.7682210003309606
2215	14	-2.6835010636837495
2215	15	-3.7870800189812215
2215	16	-3.2786951986377275
2215	17	-2.932285895993523
2215	18	-3.5004148819705385
2215	19	-4.085428271578014
2215	20	-3.729574453034971
2215	21	-2.8496412069857513
2215	22	-3.9581116509392125
2215	23	-3.8931863372055204
2215	24	-2.9185911090288528
2215	25	-3.198572110191879
2216	1	-1.8601347097062408
2216	2	-1.6726016010829916
2216	3	-2.059732991898972
2216	4	-1.6168713047194956
2216	5	-1.5698760119968327
2216	6	-2.078271897513879
2216	7	-3.3740607223035255
2216	8	-2.6485396041197724
2216	9	-2.730874794687832
2216	10	-2.826451067670181
2216	11	-3.2792612228372064
2216	12	-2.63773107565693
2216	13	-3.2404287140868355
2216	14	-3.147400702027255
2216	15	-3.1506015139335943
2216	16	-2.9203538856479057
2216	17	-3.224876817914229
2216	18	-2.9023534768651444
2216	19	-3.1869558811894763
2216	20	-3.6112592289055456
2216	21	-3.4584507477532256
2216	22	-3.8667406272482054
2216	23	-3.0756850380383085
2216	24	-3.366153554786976
2216	25	-3.096937006034281
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47398.0
3	0.0
4	788.0
5	6891.0
6	7946.0
7	6253.0
8	4984.0
9	4276.0
10	3756.0
11	3368.0
12	3141.0
13	2930.0
14	2806.0
15	2831.0
16	2961.0
17	3107.0
18	3259.0
19	4141.0
20	5281.0
21	6739.0
22	8400.0
23	10285.0
24	11817.0
25	14004.0
26	15795.0
27	17510.0
28	19778.0
29	23710.0
30	29679.0
31	38000.0
32	48567.0
33	62252.0
34	81364.0
35	107350.0
36	146841.0
37	229448.0
38	764274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.68850436234077	20.044717348994688	14.027240565970372	27.23953772269417
2	13.144817429920144	20.537921035657817	41.16665620201721	25.150605332404837
3	16.83617496132836	24.625983914882443	30.46286095905658	28.07498016473261
4	11.182766230169944	17.431492282087078	37.8744408188667	33.511300668876274
5	12.216015216683516	38.63883795717166	36.53129301826116	12.61385380788366
6	31.92787086403123	37.827065792187156	17.68158960130292	12.563473742478692
7	27.698044041191906	31.79787759483588	22.485161150335607	18.01891721363661
8	24.340654005426245	38.45797515350564	20.326488647722403	16.87488219334571
9	25.885452150092142	14.875356273817022	20.525883282099194	38.713308293991645
10	14.417916554287254	29.330340612471602	35.299573034426956	20.95216979881419
11	33.414846184450795	22.19096040747292	25.882385175620808	18.511808232455472
12	23.435835547370502	24.753468341076278	32.24521065797721	19.565485453576006
13	28.638338194916173	22.464108960344326	25.963934856692717	22.933617988046787
14	19.893721290391817	23.141678116735328	27.78014041485116	29.18446017802169
15	22.01105601876007	33.06730311461429	23.059402696417298	21.862238170208347
16	21.042923350425944	28.47107329499199	29.19668149853223	21.28932185604984
17	20.8382887540545	29.739261605676216	28.6205878675314	20.801861772737887
18	20.779913157788084	27.559816041093367	31.35515290999039	20.305117891128155
19	23.134198007390943	27.187800035847324	28.099509439605818	21.578492517155915
20	23.29291501650259	27.83737839969952	28.66056719597499	20.2091393878229
21	21.72015256352152	27.230259725582016	27.825490346053428	23.224097364843036
22	22.643206758280176	29.14131598099234	27.49449890834368	20.720978352383806
23	20.806481994143603	28.867534661773014	28.772325378297076	21.553657965786304
24	22.744599093113415	27.29632724632519	29.2458365868917	20.713237073669692
25	21.78703471675582	28.100370862333413	28.75321871630373	21.35937570460704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	143.0
1	143.0
2	279.5
3	416.0
4	416.0
5	416.0
6	1098.5
7	1781.0
8	1781.0
9	1781.0
10	2471.5
11	3162.0
12	3162.0
13	3162.0
14	5583.5
15	8005.0
16	8005.0
17	8005.0
18	14811.0
19	21617.0
20	21617.0
21	21617.0
22	35326.5
23	49036.0
24	49036.0
25	49036.0
26	71133.0
27	93230.0
28	93230.0
29	93230.0
30	120086.0
31	146942.0
32	146942.0
33	146942.0
34	165939.0
35	184936.0
36	184936.0
37	184936.0
38	196085.5
39	207235.0
40	207235.0
41	207235.0
42	214948.5
43	222662.0
44	222662.0
45	222662.0
46	227336.5
47	232011.0
48	232011.0
49	232011.0
50	217678.5
51	203346.0
52	203346.0
53	203346.0
54	176675.0
55	150004.0
56	150004.0
57	150004.0
58	127446.0
59	104888.0
60	104888.0
61	104888.0
62	84713.5
63	64539.0
64	64539.0
65	64539.0
66	49293.0
67	34047.0
68	34047.0
69	34047.0
70	24712.0
71	15377.0
72	15377.0
73	15377.0
74	10535.0
75	5693.0
76	5693.0
77	5693.0
78	3852.0
79	2011.0
80	2011.0
81	2011.0
82	1315.0
83	619.0
84	619.0
85	619.0
86	400.0
87	181.0
88	181.0
89	181.0
90	111.0
91	41.0
92	41.0
93	41.0
94	24.5
95	8.0
96	8.0
97	8.0
98	4.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07871319059551465
2	0.0
3	0.0
4	0.027683754487907622
5	0.0696374855159738
6	0.08008310834336989
7	0.03510414228879008
8	0.06735428926954845
9	0.04674844314555947
10	0.10879430114216893
11	0.0033677144634774218
12	0.013014218604624615
13	0.005707990616063427
14	0.006906668645436746
15	0.00707790836391865
16	0.016895652223547743
17	0.027455434863265085
18	9.703584047307826E-4
19	0.0031964747449955196
20	0.004794712117493279
21	2.2831962464253707E-4
22	2.8539953080317136E-4
23	0.0
24	1.1415981232126854E-4
25	0.0038243537127624965
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1751930.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.55136691357232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.72086375174509	59.066213768705644
2	11.283327194878458	15.921083138635176
3	2.7327454599983656	5.783967828892309
4	0.9283478455720834	2.619848379055217
5	0.4158737917004889	1.467023223399987
6	0.22634637059891616	0.9581427505007731
7	0.1442687127115753	0.7124848419264173
8	0.09683154669753864	0.5465278383893408
9	0.06824017583947316	0.43329939155076264
>10	0.33648565615446957	4.166181876051085
>50	0.023246147042297362	1.153493065733645
>100	0.018966274935222353	2.751446083447253
>500	0.0022690549004847544	1.0800015107357925
>1k	0.0021069795504501288	3.0508756281568745
>5k	8.103767501731262E-5	0.28941067481972377
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	5062	0.2889384849851307	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4809	0.2744972687264902	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4305	0.24572899602153053	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4247	0.24241836146421375	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3911	0.22323951299424066	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2987	0.17049767970181456	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2855	0.16296313208861082	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2425	0.13841877243953812	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2253	0.12860102857990902	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2200	0.1255757935533954	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2068	0.11804124594019168	No Hit
CCCATGTACTCTGCGTTGATACCAC	2065	0.11787000622170977	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1796	0.10251551146449915	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	5.7079906160634274E-5	0.0	0.0	0.0	0.0
11	5.7079906160634274E-5	0.0	0.0	0.0	0.0
12	5.7079906160634274E-5	0.0	0.0	0.0	0.0
13	5.7079906160634274E-5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	840	0.0	12.573215	4
ATAGGCG	55	0.003032985	12.109827	5
ACATCGG	55	0.0030577737	12.096675	8
TCGAACT	90	7.543793E-6	11.599739	19
GCCTTAC	100	1.90828E-6	11.410654	1
TGTCGAG	125	1.8586434E-8	11.388835	18
GGCGAGG	1180	0.0	11.340576	19
TGTAGGA	2830	0.0	10.95056	2
AGGACGT	2715	0.0	10.934209	5
TAGGGGT	140	8.1490725E-9	10.874131	4
ATGTCGA	140	8.463758E-9	10.847748	17
TATTGCG	70	0.0015023763	10.847748	16
CCACCTT	640	0.0	10.826561	13
GGACGTG	2665	0.0	10.814622	6
GTATTAG	195	1.8189894E-12	10.727965	1
TTAGGAC	1590	0.0	10.70221	3
GTCCACG	80	3.7387692E-4	10.69749	1
AATGTCC	725	0.0	10.6188545	8
GTCCTAG	260	0.0	10.606057	1
GTAGGAC	2780	0.0	10.532307	3
>>END_MODULE