FastQCFastQC Report
Thu 26 May 2016
SRR1512961_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512961_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201899
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC65690.29833339313020263No Hit
CCCATGTACTCTGCGTTGATACCAC50230.22812127168412355No Hit
GTCCTACAGTGGACATTTCTAAATT46190.2097734728068817No Hit
CTGTAGGACGTGGAATATGGCAAGA38750.1759844570527531No Hit
GTATCAACGCAGAGTACTTTTTTTT36410.16535726661395458No Hit
GTCCTAAAGTGTGTATTTCTCATTT33500.15214140158108977No Hit
GTCCTACAGTGTGCATTTCTCATTT33450.15191432486231202No Hit
CTTTAGGACGTGAAATATGGCGAGG30160.13697267676673633No Hit
GAGTACATGGGAAGCAGTGGTATCA27250.12375681173387154No Hit
CTGTAGGACCTGGAATATGGCGAGA23120.1050002747628297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1950.013.64522118
GTAGGAC21100.012.1524013
TAGGACC7750.011.8864124
TGTAGGA23500.011.7599622
GGACCGA658.064809E-411.6855436
GTCCTAC23500.011.3140871
CCAACGA1850.011.29101819
CTGTAGG23300.011.24760151
GGCGAGG9200.011.14597419
CTACACG951.3657658E-510.9963844
TAGGACT4850.010.9654664
AGGACCG1303.285095E-810.9576855
GACGTGG10250.010.9302587
AGGACGT19600.010.9017785
GGACGTG19000.010.8936936
TCCTACA25700.010.716322
TAGGACG20000.010.6839864
TGGCGAG21650.010.53441718
AGGACCT13900.010.521485
GTCCTAA11650.010.4704941
Produced by FastQC (version 0.11.5)