FastQCFastQC Report
Thu 26 May 2016
SRR1512961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201899
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC64540.29311062859831444No Hit
CCCATGTACTCTGCGTTGATACCAC58120.2639539779072519No Hit
GTATCAACGCAGAGTACTTTTTTTT48240.21908361827676928No Hit
GTCCTACAGTGGACATTTCTAAATT45330.20586775324390447No Hit
CTGTAGGACGTGGAATATGGCAAGA38380.17430408933379776No Hit
GTCCTAAAGTGTGTATTTCTCATTT32610.1480994359868459No Hit
GTCCTACAGTGTGCATTTCTCATTT32470.1474636211742682No Hit
TATCAACGCAGAGTACTTTTTTTTT32350.1469186370492016No Hit
CTTTAGGACGTGAAATATGGCGAGG32110.14582866879906844No Hit
GGTATCAACGCAGAGTACTTTTTTT27680.12570967151536017No Hit
GAGTACATGGGAAGCAGTGGTATCA24020.10908765570082915No Hit
CTGTAGGACCTGGAATATGGCGAGA23100.10490944407531863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8350.012.7614624
CCAACGT1302.0190782E-1012.41103519
TCCAACG2650.011.81872118
TAGACAG3650.011.7297445
ATTATAC2000.011.4086413
GTCCTAC22600.011.2729991
GCCGGTT1950.011.1968111
TGTAGGA24300.010.9545492
AGAACCG1053.406556E-610.8732865
GACGTGG10200.010.7154887
GTAGGAC23550.010.5770123
CTGTAGG24400.010.5582661
GATATAC6850.010.5471751
CCAACGA1803.6379788E-1110.54532419
TCCTACA26050.010.4011142
AACAGCG1106.0161346E-610.3681897
ATCCCGT1659.295036E-1010.36394610
ACTAGAC1303.8032704E-710.2385233
TGTAGAA8450.010.2382912
AGGACCT14700.010.2260685
Produced by FastQC (version 0.11.5)