##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512961_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2201899
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.408468326658035	31.0	31.0	34.0	27.0	34.0
2	30.518765392963076	31.0	31.0	34.0	27.0	34.0
3	30.62985268624946	31.0	31.0	34.0	27.0	34.0
4	34.10909991784364	37.0	35.0	37.0	30.0	37.0
5	33.76629627426144	37.0	35.0	37.0	28.0	37.0
6	33.63477025967131	37.0	35.0	37.0	28.0	37.0
7	33.49398950633067	37.0	35.0	37.0	28.0	37.0
8	33.370839443589375	37.0	35.0	37.0	27.0	37.0
9	34.81267260669086	39.0	35.0	39.0	27.0	39.0
10	34.691470862196674	39.0	35.0	39.0	27.0	39.0
11	34.74485705293476	39.0	35.0	39.0	27.0	39.0
12	34.58269975144182	39.0	35.0	39.0	27.0	39.0
13	34.572350957060245	39.0	35.0	39.0	27.0	39.0
14	35.61230419742232	40.0	36.0	41.0	27.0	41.0
15	35.62518716798545	40.0	36.0	41.0	27.0	41.0
16	35.59242226823301	40.0	36.0	41.0	27.0	41.0
17	35.57652916868576	40.0	36.0	41.0	27.0	41.0
18	35.49752372838173	40.0	36.0	41.0	27.0	41.0
19	35.4785932506441	40.0	36.0	41.0	26.0	41.0
20	35.45350263567948	40.0	36.0	41.0	26.0	41.0
21	35.398008718837694	40.0	36.0	41.0	26.0	41.0
22	35.355866458906604	40.0	36.0	41.0	25.0	41.0
23	35.25620929933662	40.0	36.0	41.0	25.0	41.0
24	35.24246116647494	40.0	36.0	41.0	25.0	41.0
25	35.14543037623433	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6030766232062028
1101	2	0.6111707464744249
1101	3	0.572660382797693
1101	4	0.39786758991046156
1101	5	0.6999702428453176
1101	6	0.6693209669950946
1101	7	0.6555913396518491
1101	8	0.6657592977361517
1101	9	0.46011957230204814
1101	10	0.8625861035854996
1101	11	0.8392717348156964
1101	12	0.892024376066253
1101	13	0.9186634197929777
1101	14	0.9406135206136454
1101	15	0.8445299844115794
1101	16	1.077894814175032
1101	17	1.0325823040213677
1101	18	0.5350422376797397
1101	19	0.9421945021084923
1101	20	0.8750639781502443
1101	21	1.0535003410861137
1101	22	1.0179151843057
1101	23	0.9369893714100712
1101	24	0.8648921791971986
1101	25	0.9658101545493594
1102	1	0.5669016530270916
1102	2	0.5588697764463149
1102	3	0.509742904668613
1102	4	0.4762641539536645
1102	5	0.6432304412105765
1102	6	0.6163966391373563
1102	7	0.6750870051966373
1102	8	0.6164604126870472
1102	9	0.8459057305060824
1102	10	1.0159186815999348
1102	11	0.9410064222266215
1102	12	0.9429539061316277
1102	13	0.8363288550671726
1102	14	0.9764620453642152
1102	15	0.9662094854932661
1102	16	1.0044567223883192
1102	17	0.9975184261045698
1102	18	1.0098449838645536
1102	19	1.0696931522469768
1102	20	1.0404999994607849
1102	21	1.0190018096950055
1102	22	1.0991033226113274
1102	23	1.0714686272269986
1102	24	1.0088959242663478
1102	25	1.220752344087579
1103	1	0.5660022681353425
1103	2	0.43393395974433346
1103	3	0.5273760538791166
1103	4	0.5571432966526757
1103	5	0.526423991838378
1103	6	0.6176635859841895
1103	7	0.5589519057963344
1103	8	0.6755893675545721
1103	9	0.7396353390047707
1103	10	0.7922702021688366
1103	11	0.7672231298608594
1103	12	0.7513742377457646
1103	13	0.8392621115040697
1103	14	0.8639258644268608
1103	15	0.6958303942443109
1103	16	0.7737337310389947
1103	17	0.8611297580784267
1103	18	0.975466866805597
1103	19	0.9294009159310974
1103	20	1.0671761207574377
1103	21	0.7011118238752303
1103	22	0.8868587436977151
1103	23	0.8119205052229717
1103	24	0.8780313720102413
1103	25	0.9635806630944685
1104	1	0.5212472119205565
1104	2	0.5703595029879835
1104	3	0.4806019496517351
1104	4	0.5076122655173023
1104	5	0.6004230668224224
1104	6	0.6533314866264348
1104	7	0.6800912301011408
1104	8	0.597284277240199
1104	9	0.707118284552287
1104	10	0.9061059310541495
1104	11	0.8813098425705874
1104	12	0.8055298406039952
1104	13	0.8165258899148213
1104	14	1.0402916220364418
1104	15	1.001822861681191
1104	16	0.9540553629148221
1104	17	0.8610542258343727
1104	18	0.9148476683720617
1104	19	0.963976308711473
1104	20	1.05158328493998
1104	21	0.7785880917626997
1104	22	0.9891301642441448
1104	23	0.886519011547442
1104	24	0.9362884942690002
1104	25	1.1323405269535272
1105	1	0.49125558343127196
1105	2	0.5089211618040892
1105	3	0.4659454371778473
1105	4	0.3819191019158623
1105	5	0.3907111088023214
1105	6	0.4820341203422558
1105	7	0.5736800271929852
1105	8	0.7265701513423934
1105	9	0.7357885718903603
1105	10	0.6893490827980457
1105	11	0.7605598088415277
1105	12	0.5632866444466771
1105	13	0.6877442338305642
1105	14	0.8613775217811082
1105	15	0.8427520909910342
1105	16	0.7831019911401356
1105	17	0.5887175855168749
1105	18	0.8118415502139271
1105	19	0.7622292691173271
1105	20	0.8798876224551577
1105	21	0.8181073206786849
1105	22	0.7743867523046504
1105	23	0.7406578814186346
1105	24	0.8971359693660617
1105	25	0.9072202126741118
1106	1	0.41321907675951053
1106	2	0.40741686054999704
1106	3	0.4081138616620237
1106	4	0.40362485923362357
1106	5	0.5172743004594551
1106	6	0.4958969253539962
1106	7	0.6439538396422506
1106	8	0.5648073363190775
1106	9	0.5074515111068578
1106	10	0.6350248682723247
1106	11	0.4370403015765163
1106	12	0.5773182396546943
1106	13	0.5296265423840012
1106	14	0.8380039683916891
1106	15	0.6916441571071417
1106	16	0.8054982161970443
1106	17	0.785865444901404
1106	18	0.8011342944694775
1106	19	0.8721131762132188
1106	20	0.9363369386480116
1106	21	0.6533141424502986
1106	22	0.7583407619405804
1106	23	0.7965698821533707
1106	24	0.790633492535413
1106	25	0.8759489329051888
1107	1	0.4168079369375164
1107	2	0.3742936986944798
1107	3	0.3132737988795924
1107	4	0.3113355161552178
1107	5	0.33428816336425626
1107	6	0.46106211563573396
1107	7	0.4564326549735682
1107	8	0.554315881352359
1107	9	0.5102939861902627
1107	10	0.6451000888252096
1107	11	0.5989261418295584
1107	12	0.5628573952808793
1107	13	0.5168482437018156
1107	14	0.7425131419340545
1107	15	0.6726113371948017
1107	16	0.5786795628303096
1107	17	0.5721542626733935
1107	18	0.7283169939868657
1107	19	0.7718552184813063
1107	20	0.7942691878154235
1107	21	0.7113785986236536
1107	22	0.8244853503307255
1107	23	0.8573432858014627
1107	24	0.8810108963380614
1107	25	0.8053827709533152
1108	1	0.3833027822983439
1108	2	0.39754860501723144
1108	3	0.33696045379879536
1108	4	0.43981462928017834
1108	5	0.5036292320713187
1108	6	0.3855553842604422
1108	7	0.3597360927656723
1108	8	0.4902004830020772
1108	9	0.4355736714674876
1108	10	0.606353945291481
1108	11	0.5794203744488726
1108	12	0.713171766801274
1108	13	0.5204183411355245
1108	14	0.6778586676526075
1108	15	0.6567049383391108
1108	16	0.6549658809368211
1108	17	0.6411374435043413
1108	18	0.6133118520318348
1108	19	0.5530250278874718
1108	20	0.5985519387516121
1108	21	0.67949506244166
1108	22	0.7728989547533871
1108	23	0.8172794553084017
1108	24	0.8489410008748877
1108	25	0.7592954377253633
1109	1	0.12339855797680954
1109	2	0.1527106986607656
1109	3	0.259335232970642
1109	4	0.3933561215206609
1109	5	0.3411509714785197
1109	6	0.07832877144016237
1109	7	0.34432938200205854
1109	8	0.487778199602964
1109	9	0.5379618820835219
1109	10	0.3876363282554962
1109	11	0.3124022421667405
1109	12	0.4528298228032597
1109	13	0.39105199104086097
1109	14	0.4159687069954501
1109	15	0.5305995412035642
1109	16	0.43229521800509474
1109	17	0.41853814244868914
1109	18	0.5017635237926612
1109	19	0.6750241018582486
1109	20	0.5415461959632637
1109	21	0.661824706064472
1109	22	0.6496156182875055
1109	23	0.5452832458636436
1109	24	0.305045490072942
1109	25	0.43913038072129496
1110	1	0.21688460935461507
1110	2	0.3009476171023593
1110	3	0.20203560791759756
1110	4	0.2988432674818853
1110	5	0.24138914596634464
1110	6	0.27989038607515226
1110	7	0.24367404475582788
1110	8	0.5210285253458053
1110	9	0.5346698064267486
1110	10	0.5463913724971476
1110	11	0.40742698382201326
1110	12	0.5436461872625387
1110	13	0.11089212265997617
1110	14	0.4286734767587106
1110	15	0.5880417917531702
1110	16	0.559101646445697
1110	17	0.46265769154356207
1110	18	0.382099723035104
1110	19	0.5710231202988183
1110	20	0.486931983635273
1110	21	0.38802378199252985
1110	22	0.4281642222143134
1110	23	0.6394045525486547
1110	24	0.4830477441612757
1110	25	0.39124412253651286
1111	1	-0.5174699064312343
1111	2	-0.3050095106149229
1111	3	-0.31888234965413176
1111	4	-0.35646284623815916
1111	5	-0.009007511586986539
1111	6	-0.2901550606627552
1111	7	-0.06598990465666787
1111	8	0.07218886325662766
1111	9	-0.36130899912652126
1111	10	-0.2416217278950583
1111	11	-0.0036052898795091437
1111	12	-0.09449892863886333
1111	13	-0.060930696164774645
1111	14	0.060147549238585896
1111	15	0.16589105577264718
1111	16	-0.18605376795536444
1111	17	0.15711256491526626
1111	18	0.17845349472736416
1111	19	0.12251663697608706
1111	20	0.03563497963033768
1111	21	0.07069383555233344
1111	22	0.06597768691365502
1111	23	0.059616130400463874
1111	24	0.14604039216359155
1111	25	0.18077086951007004
1112	1	0.11507961379934883
1112	2	-0.17545534658918882
1112	3	0.05699190849245639
1112	4	-0.05611990077962048
1112	5	0.212792601283752
1112	6	0.09924459907427519
1112	7	0.013970948249919957
1112	8	0.23488393240113226
1112	9	0.04303330872583189
1112	10	0.31382679572336514
1112	11	0.17751475788249138
1112	12	0.2854120403540108
1112	13	0.2806946699687032
1112	14	0.2960539249908436
1112	15	0.22403848655334713
1112	16	0.030469758302558603
1112	17	0.12924859031771518
1112	18	0.12038206770352389
1112	19	0.250525018711663
1112	20	0.40522585931707766
1112	21	0.545304023976307
1112	22	0.38241881271844846
1112	23	0.565806653591757
1112	24	0.5189083750135666
1112	25	0.5511504171398371
1113	1	-0.16877634034004885
1113	2	-0.5836956081363383
1113	3	-0.1239573936922902
1113	4	-0.13614946806826111
1113	5	-0.12322193497944767
1113	6	-0.19395667583526688
1113	7	0.0022503796016195565
1113	8	0.14508568760567186
1113	9	0.09918019219333729
1113	10	-0.18784425355412537
1113	11	0.03565750947623059
1113	12	-0.04855779454786102
1113	13	0.06117853355409153
1113	14	0.09266223176778965
1113	15	0.15239576512080788
1113	16	-0.2292790584940434
1113	17	-0.0012192281827196894
1113	18	0.16877358706604184
1113	19	0.038674397310437314
1113	20	0.20696038672397066
1113	21	-0.08802741705506634
1113	22	-0.19978646470585204
1113	23	-0.15782109400742428
1113	24	-0.41798279057873344
1113	25	-0.34404137894434683
1114	1	-0.7429022079900172
1114	2	-0.512867252346183
1114	3	-0.49718616704885576
1114	4	-0.2616042660831326
1114	5	-0.5184615094994811
1114	6	-0.9586500044541353
1114	7	-0.6550440257661307
1114	8	-0.8951155187908384
1114	9	-0.7835657279049926
1114	10	-0.838471992255208
1114	11	-0.896689491580311
1114	12	-0.8821412467950154
1114	13	-0.7369719671467578
1114	14	-1.9122857618508533
1114	15	-1.1611012273065953
1114	16	-1.0903232270864862
1114	17	-1.2345894353242883
1114	18	-1.3114612940411803
1114	19	-1.2516729152371795
1114	20	-0.5169966443451557
1114	21	-0.5936096738499899
1114	22	-0.6914379146126137
1114	23	-0.6722703230670177
1114	24	-1.3843141413134674
1114	25	-1.1807762789935978
1115	1	-1.3201362588249417
1115	2	-1.0837561498848807
1115	3	-0.7763505955776537
1115	4	-2.039873179969973
1115	5	-1.256272156548384
1115	6	-1.1044455489379388
1115	7	-0.7529935022364853
1115	8	-0.7861134039127364
1115	9	-1.235621571066666
1115	10	-1.014708547209601
1115	11	-0.80750495224747
1115	12	-1.3798709316392603
1115	13	-0.9213013691422702
1115	14	-1.669605186292614
1115	15	-1.6780622901950224
1115	16	-1.0823967158568735
1115	17	-1.0876879263652484
1115	18	-0.9591817668396985
1115	19	-0.7973132821620439
1115	20	-0.8625300996407859
1115	21	-0.8941489028925886
1115	22	-1.2037439102271605
1115	23	-1.530793541314651
1115	24	-0.9217739521421677
1115	25	-0.8971957773912251
1116	1	-0.463782321979739
1116	2	-0.4807034157419423
1116	3	-0.32283164957646804
1116	4	-0.4125812870783392
1116	5	-0.43833760078572226
1116	6	-1.0823280622204763
1116	7	-0.4782377846715775
1116	8	-0.6124181819622407
1116	9	-0.7386854249820374
1116	10	-0.6797392619337828
1116	11	-0.6017957271643084
1116	12	-1.2421728931119986
1116	13	-0.9227197885103848
1116	14	-1.1816788925532933
1116	15	-0.9964342614956365
1116	16	-1.1436877344037129
1116	17	-1.0288984513757384
1116	18	-1.6449794670567925
1116	19	-1.101539638583823
1116	20	-0.9315035679610233
1116	21	-0.9748067233426241
1116	22	-2.198125993712665
1116	23	-2.114642379841456
1116	24	-1.3879725185344185
1116	25	-1.6712611289097623
1201	1	0.7077367051390127
1201	2	0.7557933229151814
1201	3	0.6409759738405576
1201	4	0.6881062210581845
1201	5	0.707934637173345
1201	6	0.7653349039181236
1201	7	0.8041399654731975
1201	8	0.7551876826190806
1201	9	0.920273055182804
1201	10	0.8020618612430752
1201	11	0.8963356782432754
1201	12	0.8550288163434274
1201	13	0.850226328628608
1201	14	0.9444450698997429
1201	15	0.9896809022897699
1201	16	0.9730371411355421
1201	17	0.9085883618456023
1201	18	0.9007085124369283
1201	19	0.7326110879796275
1201	20	0.8803811425583206
1201	21	0.8614258308297806
1201	22	0.9992716656621852
1201	23	0.9031816557841736
1201	24	1.0309282315968886
1201	25	1.035552451564378
1202	1	0.6314931167658848
1202	2	0.7228463805683383
1202	3	0.6564075076208944
1202	4	0.6660958005743112
1202	5	0.7234216680902037
1202	6	0.8143210310000768
1202	7	0.6648678522549432
1202	8	0.6182160537798325
1202	9	0.8533176383378347
1202	10	0.8968154522493279
1202	11	0.8730326013795775
1202	12	0.8468064459770517
1202	13	0.937302787289731
1202	14	0.8233127507239288
1202	15	1.0959510883059238
1202	16	0.8721948312115302
1202	17	1.0053603410169316
1202	18	1.046812152400193
1202	19	1.2497254656996262
1202	20	1.210343807500081
1202	21	1.2658870834121103
1202	22	1.0983347373894148
1202	23	1.1107066235189933
1202	24	0.8994351093143251
1202	25	0.9919520227508158
1203	1	0.5686619402292195
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2211	10	-0.3623365595394148
2211	11	-0.10274312854748047
2211	12	-0.11466313623922986
2211	13	-0.10663388640320903
2211	14	-1.1511528969954696
2211	15	-0.5143453744637796
2211	16	-0.19828209121535423
2211	17	-0.039395217394357473
2211	18	-0.21331842079327146
2211	19	-0.18915415733005148
2211	20	-0.28903702864739245
2211	21	-0.35078602949239723
2211	22	-0.08074008288889445
2211	23	-0.21526833140943324
2211	24	-0.2970875558162476
2211	25	-0.3189597369621069
2212	1	-0.08452197661810246
2212	2	-0.07375722058520395
2212	3	-0.012618863923560042
2212	4	0.12851455880194607
2212	5	-0.042614969959331006
2212	6	0.039700820133568016
2212	7	0.03828278190441381
2212	8	-0.2299016794295241
2212	9	-0.23910283538430832
2212	10	-0.16948070277597793
2212	11	-0.05347704821521404
2212	12	0.07927870438641804
2212	13	-0.15072021698878046
2212	14	-0.04435922924938751
2212	15	-0.04693921740245344
2212	16	-0.023675393978244585
2212	17	-0.17788316036259033
2212	18	0.03786602576998632
2212	19	0.029022356961426965
2212	20	-0.20736261838293046
2212	21	-0.032540704202581594
2212	22	-0.0636481731197236
2212	23	-0.45905015629089974
2212	24	-0.16751047702990718
2212	25	-0.3754111929415842
2213	1	-0.3917539425473926
2213	2	-0.4768416261147692
2213	3	-0.40999851345601357
2213	4	-0.3860297914507953
2213	5	-0.16008507400740513
2213	6	-0.048656799935784534
2213	7	-0.7726741549657561
2213	8	-0.4186293384071291
2213	9	-0.5811240557891182
2213	10	-0.5106897553877943
2213	11	-0.4336498170796048
2213	12	-0.6797297483272047
2213	13	-0.5220375499554706
2213	14	-0.4892701920774982
2213	15	-0.5163340473459996
2213	16	-0.8735338571002913
2213	17	-0.6946255069375482
2213	18	-0.8607946957567165
2213	19	-1.0021771057841988
2213	20	-0.43811306932347094
2213	21	-1.176561904605741
2213	22	-0.6392156211589324
2213	23	-0.6354639430072666
2213	24	-0.8221684786685302
2213	25	-0.7997754199421436
2214	1	-1.2087371396267237
2214	2	-1.117000883735539
2214	3	-0.9194841084341121
2214	4	-1.553257021999066
2214	5	-1.4219772478274564
2214	6	-2.782598100378795
2214	7	-1.4222422550664433
2214	8	-2.0029742331817424
2214	9	-1.4310099856969956
2214	10	-1.6844391090922883
2214	11	-1.3264548826906548
2214	12	-1.5465536222926985
2214	13	-1.7334844471404125
2214	14	-1.8261082421531398
2214	15	-2.1563929121608467
2214	16	-1.7266943509252357
2214	17	-1.8877486087038307
2214	18	-1.7666685002950686
2214	19	-3.468347915126955
2214	20	-2.118361264742596
2214	21	-2.6470968852184455
2214	22	-2.4669944989611494
2214	23	-2.323948475472406
2214	24	-1.752872052804939
2214	25	-1.4910393707516434
2215	1	-1.4013968033549702
2215	2	-1.2381749766988328
2215	3	-1.2256985220665086
2215	4	-1.0246720816458534
2215	5	-1.842972899795253
2215	6	-1.5594853658959593
2215	7	-1.6539473123981523
2215	8	-2.4876441863783754
2215	9	-2.0446567406437026
2215	10	-1.3798967110607023
2215	11	-1.841696836621857
2215	12	-2.715076851609858
2215	13	-2.0386505832093818
2215	14	-2.1366984874094044
2215	15	-3.1420966448776824
2215	16	-2.6195047482774783
2215	17	-2.2074627717271866
2215	18	-3.060626590364272
2215	19	-3.751671161197411
2215	20	-3.060299501237438
2215	21	-2.211013694986171
2215	22	-3.0513423194449913
2215	23	-2.954567238149565
2215	24	-1.823370467915673
2215	25	-2.2015090291087063
2216	1	-1.1253861558894869
2216	2	-1.0671667408121372
2216	3	-1.5057898369536744
2216	4	-1.011683491998582
2216	5	-1.0083928641047777
2216	6	-1.468912256313196
2216	7	-2.7492689386261553
2216	8	-1.8801495428444568
2216	9	-1.7996895029324023
2216	10	-1.909631455208455
2216	11	-2.4654371930901675
2216	12	-1.7031398261041275
2216	13	-2.12976791241816
2216	14	-2.289639522415378
2216	15	-2.4017497961631733
2216	16	-2.0870649386563684
2216	17	-2.3358904980693325
2216	18	-2.0009023609672383
2216	19	-2.25031511018328
2216	20	-2.7057364957912355
2216	21	-2.4857773580385114
2216	22	-2.913395737585141
2216	23	-2.0366598739495103
2216	24	-2.187368157431699
2216	25	-2.015313146562171
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57804.0
3	1.0
4	884.0
5	8159.0
6	9726.0
7	7554.0
8	5983.0
9	5316.0
10	4651.0
11	4195.0
12	3847.0
13	3672.0
14	3451.0
15	3473.0
16	3679.0
17	3930.0
18	4267.0
19	4998.0
20	6436.0
21	8638.0
22	10484.0
23	12979.0
24	15397.0
25	18019.0
26	20426.0
27	22419.0
28	25544.0
29	30573.0
30	38586.0
31	49234.0
32	62374.0
33	80833.0
34	105495.0
35	137332.0
36	188319.0
37	295787.0
38	937434.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.536050780791946	18.846269569794046	13.213923085243993	27.403756564170017
2	13.64704450433217	19.936436656012223	40.39165301934967	26.024865820305937
3	17.627784017341394	24.120770298728505	29.549311753173058	28.70213393075704
4	11.374136519620983	16.77039485459731	36.92138978206177	34.93407884371994
5	12.604230436582764	38.21650116598108	35.93525953134423	13.244008866091924
6	32.835175763371446	36.98252956357088	17.37280778702048	12.809486886037194
7	28.303824957861405	31.21350070647458	21.711758372064498	18.77091596359952
8	25.39540502144608	37.63030573504296	19.701218231941255	17.273071011569705
9	26.301187493042477	14.772758768751235	19.471977935847658	39.45407580235863
10	14.431926163359021	29.34624565232217	34.551637909477364	21.67019027484144
11	34.77218835342223	22.171435422974962	24.001940233363932	19.054435990238883
12	23.383203438507337	24.597109535426338	31.69732014990555	20.322366876160775
13	29.34430324555587	21.503737884802295	25.45508633923462	23.696872530407216
14	20.612030504784144	22.08108720642143	27.175119098282142	30.131763190512288
15	23.311959465044687	30.987612603406195	22.858411975179788	22.84201595636933
16	22.0420051036955	28.002645418529376	27.859200505827282	22.096148971947844
17	21.735118994890776	28.635500721614783	27.76683733753446	21.86254294595998
18	21.78864749876923	27.353947270418498	29.673741845589852	21.18366338522242
19	23.482469613967265	26.89753178486662	27.445847416290235	22.174151184875882
20	23.6257285506469	27.197301484283564	27.94041973782396	21.23655022724558
21	22.70939349969004	26.718304006321823	27.196891768226916	23.37541072576122
22	22.93357445907932	27.95597789902693	26.949617011536432	22.16083063035732
23	21.980118070810693	27.696683635352937	27.650269154034767	22.6729291398016
24	23.03189201684546	27.082259449684116	28.05251285367767	21.83333567979276
25	22.583630966438612	27.054849269875415	27.689688875001362	22.671830888684614
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	99.0
1	99.0
2	261.5
3	424.0
4	424.0
5	424.0
6	1080.5
7	1737.0
8	1737.0
9	1737.0
10	2504.5
11	3272.0
12	3272.0
13	3272.0
14	5469.0
15	7666.0
16	7666.0
17	7666.0
18	14746.5
19	21827.0
20	21827.0
21	21827.0
22	35942.5
23	50058.0
24	50058.0
25	50058.0
26	73282.5
27	96507.0
28	96507.0
29	96507.0
30	127630.5
31	158754.0
32	158754.0
33	158754.0
34	180644.5
35	202535.0
36	202535.0
37	202535.0
38	220795.0
39	239055.0
40	239055.0
41	239055.0
42	254551.0
43	270047.0
44	270047.0
45	270047.0
46	287212.0
47	304377.0
48	304377.0
49	304377.0
50	292560.5
51	280744.0
52	280744.0
53	280744.0
54	248134.5
55	215525.0
56	215525.0
57	215525.0
58	185566.0
59	155607.0
60	155607.0
61	155607.0
62	127667.0
63	99727.0
64	99727.0
65	99727.0
66	76892.5
67	54058.0
68	54058.0
69	54058.0
70	39428.0
71	24798.0
72	24798.0
73	24798.0
74	17360.5
75	9923.0
76	9923.0
77	9923.0
78	6775.0
79	3627.0
80	3627.0
81	3627.0
82	2381.5
83	1136.0
84	1136.0
85	1136.0
86	714.0
87	292.0
88	292.0
89	292.0
90	193.5
91	95.0
92	95.0
93	95.0
94	51.5
95	8.0
96	8.0
97	8.0
98	4.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07706983835316697
2	4.541534375554919E-5
3	0.0
4	0.028747912597262636
5	0.072937042071412
6	0.07834146797832235
7	0.037649319973350276
8	0.06798676960205714
9	0.04741361888079335
10	0.11122217685733997
11	0.004632365063066017
12	0.0111721745638651
13	0.005404425906910353
14	0.006630640188310182
15	0.006585224844554633
16	0.01621327772073106
17	0.026613391440751824
18	0.0010445529063776314
19	0.002951997344110697
20	0.0043598730005327225
21	1.8166137502219676E-4
22	2.2707671877774595E-4
23	0.0
24	0.0
25	0.0037694735317105824
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2201899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.12104335226644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.3371261304516	56.91228064763975
2	11.819999830236338	16.340214413790953
3	3.1507558454029243	6.533505941475072
4	1.0991955281162893	3.0391016700617373
5	0.5168607010210556	1.7862975461179604
6	0.289938017583854	1.202449096973026
7	0.1669986214964286	0.8080183269356364
8	0.11069975813537229	0.6121346224928382
9	0.07951581253576225	0.4946594332927602
>10	0.3856777169443204	4.607438711105774
>50	0.02334231130205684	1.0915826700300333
>100	0.015545646599381143	2.224491128163532
>500	0.002435317673628285	1.1843272365492046
>1k	0.0017771237077824288	2.605456227332733
>5k	1.316387931690688E-4	0.5580423280389618
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	6454	0.29311062859831444	No Hit
CCCATGTACTCTGCGTTGATACCAC	5812	0.2639539779072519	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4824	0.21908361827676928	No Hit
GTCCTACAGTGGACATTTCTAAATT	4533	0.20586775324390447	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3838	0.17430408933379776	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3261	0.1480994359868459	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3247	0.1474636211742682	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3235	0.1469186370492016	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3211	0.14582866879906844	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2768	0.12570967151536017	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2402	0.10908765570082915	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2310	0.10490944407531863	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.541534375554919E-5	0.0	0.0	0.0
3	0.0	4.541534375554919E-5	0.0	0.0	0.0
4	0.0	4.541534375554919E-5	0.0	0.0	0.0
5	0.0	4.541534375554919E-5	0.0	0.0	0.0
6	0.0	4.541534375554919E-5	0.0	0.0	4.541534375554919E-5
7	0.0	4.541534375554919E-5	0.0	0.0	4.541534375554919E-5
8	0.0	4.541534375554919E-5	0.0	4.541534375554919E-5	4.541534375554919E-5
9	0.0	4.541534375554919E-5	0.0	4.541534375554919E-5	4.541534375554919E-5
10	0.0	4.541534375554919E-5	0.0	9.083068751109838E-5	4.541534375554919E-5
11	0.0	1.3624603126664755E-4	0.0	9.083068751109838E-5	4.541534375554919E-5
12	0.0	1.3624603126664755E-4	0.0	9.083068751109838E-5	9.083068751109838E-5
13	0.0	1.3624603126664755E-4	0.0	9.083068751109838E-5	9.083068751109838E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	835	0.0	12.761462	4
CCAACGT	130	2.0190782E-10	12.411035	19
TCCAACG	265	0.0	11.818721	18
TAGACAG	365	0.0	11.729744	5
ATTATAC	200	0.0	11.408641	3
GTCCTAC	2260	0.0	11.272999	1
GCCGGTT	195	0.0	11.19681	11
TGTAGGA	2430	0.0	10.954549	2
AGAACCG	105	3.406556E-6	10.873286	5
GACGTGG	1020	0.0	10.715488	7
GTAGGAC	2355	0.0	10.577012	3
CTGTAGG	2440	0.0	10.558266	1
GATATAC	685	0.0	10.547175	1
CCAACGA	180	3.6379788E-11	10.545324	19
TCCTACA	2605	0.0	10.401114	2
AACAGCG	110	6.0161346E-6	10.368189	7
ATCCCGT	165	9.295036E-10	10.363946	10
ACTAGAC	130	3.8032704E-7	10.238523	3
TGTAGAA	845	0.0	10.238291	2
AGGACCT	1470	0.0	10.226068	5
>>END_MODULE