FastQCFastQC Report
Thu 26 May 2016
SRR1512960_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512960_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2216367
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT51080.23046724662476925No Hit
CTGTAGGACGTGGAATATGGCAAGA46870.21147219751963459No Hit
GTCCTAAAGTGTGTATTTCTCATTT42380.19121381973292328No Hit
CTTTAGGACGTGAAATATGGCGAGG38820.17515149792430587No Hit
GTACATGGGAAGCAGTGGTATCAAC33950.1531786026411691No Hit
CCCATGTACTCTGCGTTGATACCAC28070.1266487003280594No Hit
GTATCAACGCAGAGTACTTTTTTTT26070.1176249240310833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAC500.001502882613.2964933
TAGGACC6150.011.736744
CTACACG951.3647916E-510.9970994
GGACCGC1158.89233E-710.7324216
AATCCCG1605.493348E-1010.68130519
TTGCGAG909.495895E-510.55801518
TGTAGGA24050.010.5442342
GTCCTAT1909.094947E-1210.5326581
TTAGGAC13950.010.4846893
GTCTAGA2200.010.3958691
GTCCTAC21050.010.3670621
GGACCTG13400.010.2734296
GCCGGTT1303.870191E-710.22553211
AGGACGT23500.010.2247315
GGACGTG23550.010.199576
AGGACCT13700.010.1211895
TCCAACG1606.151822E-910.09610218
CTGTAGG23450.010.03751851
GGCGAGG10150.010.00897419
GACGTGG12300.09.8800027
Produced by FastQC (version 0.11.5)