##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512960_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2216367 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.278479601979274 31.0 30.0 34.0 23.0 34.0 2 29.3648633100926 31.0 30.0 34.0 23.0 34.0 3 29.49979673943891 31.0 30.0 34.0 23.0 34.0 4 32.93188312224465 35.0 35.0 37.0 26.0 37.0 5 33.0627644248448 37.0 35.0 37.0 26.0 37.0 6 33.040321842005405 37.0 35.0 37.0 26.0 37.0 7 32.93859590943197 37.0 35.0 37.0 26.0 37.0 8 32.911036394243375 37.0 35.0 37.0 26.0 37.0 9 34.25253669631429 39.0 35.0 39.0 25.0 39.0 10 34.12557441975991 39.0 35.0 39.0 25.0 39.0 11 34.21330808480725 39.0 35.0 39.0 25.0 39.0 12 34.10190685928819 39.0 35.0 39.0 25.0 39.0 13 34.114852368763835 39.0 35.0 39.0 25.0 39.0 14 35.131008086657125 40.0 35.0 41.0 25.0 41.0 15 35.08264019451652 40.0 35.0 41.0 24.0 41.0 16 34.96203787549625 39.0 35.0 41.0 23.0 41.0 17 34.910540988924666 39.0 35.0 41.0 23.0 41.0 18 34.60678714310401 39.0 34.0 41.0 18.0 41.0 19 34.84214753242581 39.0 34.0 41.0 21.0 41.0 20 34.928220371445704 40.0 35.0 41.0 22.0 41.0 21 34.8671239916494 40.0 35.0 41.0 22.0 41.0 22 34.78114725584707 39.0 35.0 41.0 19.0 41.0 23 34.510437576448304 39.0 34.0 41.0 18.0 41.0 24 34.523323980189204 39.0 34.0 41.0 18.0 41.0 25 34.442265202468725 39.0 34.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8179273470243764 1101 2 1.057283524349515 1101 3 1.001716381988654 1101 4 0.9775660590436743 1101 5 0.9167969798445625 1101 6 0.9585866201215794 1101 7 0.9856226117791351 1101 8 0.8444239889321921 1101 9 0.7543503846929056 1101 10 0.8670239882811615 1101 11 1.0249911871707056 1101 12 1.1896897941222448 1101 13 1.0597048991352267 1101 14 1.023606772346369 1101 15 0.8006752365178471 1101 16 1.395454652999895 1101 17 1.4069358792056406 1101 18 1.6350610427742112 1101 19 1.1798710423149075 1101 20 1.3027452783833056 1101 21 1.2439484576550228 1101 22 1.184772876307406 1101 23 1.5007027355692273 1101 24 1.1667431024452384 1101 25 1.3653389019568039 1102 1 0.6889437378927568 1102 2 0.7161517403324638 1102 3 0.6479299160679233 1102 4 0.7904701220243453 1102 5 0.7314542065401142 1102 6 0.5506411709665215 1102 7 0.8587167045970645 1102 8 0.7982583806414922 1102 9 0.7693834149403358 1102 10 1.0063720376855798 1102 11 0.9264909019785179 1102 12 1.000974185716089 1102 13 0.7806259979751502 1102 14 0.8916867760838016 1102 15 1.0788141847119874 1102 16 1.116517504172208 1102 17 0.970167633433384 1102 18 1.1803859957854428 1102 19 1.163275983076467 1102 20 1.2218829445892467 1102 21 0.9769872492998743 1102 22 1.2755885153199884 1102 23 1.4836670539492331 1102 24 1.3926793561726782 1102 25 1.1690788009929065 1103 1 0.3184114323174718 1103 2 0.5195575387520321 1103 3 0.4911070129477082 1103 4 0.6546355214938373 1103 5 0.20886461902583164 1103 6 0.5613781292812163 1103 7 0.4143816686691295 1103 8 0.46143577580364337 1103 9 0.7471252570684399 1103 10 0.6890828761202457 1103 11 0.7935917965066466 1103 12 0.9139093557676361 1103 13 0.8316515074108324 1103 14 1.024706918678291 1103 15 1.0766311198572112 1103 16 1.097084235498329 1103 17 1.0078734558103761 1103 18 1.2096839623704057 1103 19 1.2709399691961636 1103 20 1.1482351314084909 1103 21 1.0017312372506453 1103 22 1.085852394351143 1103 23 1.4968262202655325 1103 24 1.237688820603232 1103 25 1.2793378437116871 1104 1 0.7095378202155267 1104 2 0.6867019029529011 1104 3 0.656968996379625 1104 4 0.9728539513763437 1104 5 0.7165403655350318 1104 6 0.7015433439089733 1104 7 0.8757879110020994 1104 8 0.918734244437978 1104 9 0.9091918697564267 1104 10 1.033466890379131 1104 11 1.0947778889250515 1104 12 1.0230870519026851 1104 13 1.011781750474519 1104 14 1.2420504429298873 1104 15 1.3834484043297834 1104 16 1.1420230500410185 1104 17 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1107 17 1.2290783884145071 1107 18 1.3736499522443353 1107 19 1.179176190115136 1107 20 1.2612236831376649 1107 21 1.2383096262540434 1107 22 1.218802558172868 1107 23 1.2587536747205306 1107 24 1.286373760787498 1107 25 1.2420522810356474 1108 1 0.6795160294903191 1108 2 0.6724589510780703 1108 3 0.5925477918688102 1108 4 0.7725242550904312 1108 5 0.7039213215556401 1108 6 0.7842798855431994 1108 7 0.8805008518525383 1108 8 0.8552862011898696 1108 9 1.0040266443809571 1108 10 0.9692077561335068 1108 11 0.8574325604196389 1108 12 0.990761841642815 1108 13 0.966760488203569 1108 14 1.085460075853888 1108 15 1.061657399689743 1108 16 1.015152441121991 1108 17 1.0241322457186683 1108 18 1.170356450934534 1108 19 1.10616964023707 1108 20 1.0561712968979577 1108 21 0.9515384767895227 1108 22 0.901071247043383 1108 23 0.9583757454775963 1108 24 1.0906436993345494 1108 25 1.178944506063516 1109 1 0.09001639669188322 1109 2 0.16487455644265125 1109 3 0.3556829331796898 1109 4 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2215 16 -2.588405410084235 2215 17 -2.792226379130213 2215 18 -1.7141997440938255 2215 19 -2.48265584082489 2215 20 -2.688997854035513 2215 21 -2.3489312277390297 2215 22 -2.148941889754866 2215 23 -2.581927307494496 2215 24 -2.422348684955523 2215 25 -2.40535344477761 2216 1 -2.251744325729689 2216 2 -2.249213571559981 2216 3 -2.6902039588277127 2216 4 -3.5143822609820354 2216 5 -2.5428666708262355 2216 6 -2.615508727379858 2216 7 -2.514629476423174 2216 8 -3.0481434184206613 2216 9 -3.3134864645108557 2216 10 -2.9161218246972673 2216 11 -3.343448712076608 2216 12 -3.876466237634329 2216 13 -4.452660500391019 2216 14 -3.7806857132914864 2216 15 -3.6743028154615374 2216 16 -2.818471009028457 2216 17 -2.7929950159328953 2216 18 -2.8245773130394234 2216 19 -3.4743541108222225 2216 20 -3.1814247870288526 2216 21 -3.3646776392728057 2216 22 -4.073574168444534 2216 23 -3.988641934907612 2216 24 -3.7368759205262805 2216 25 -3.813046969540501 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99867.0 3 1.0 4 798.0 5 5102.0 6 6217.0 7 5588.0 8 5008.0 9 4653.0 10 4243.0 11 4017.0 12 3973.0 13 3829.0 14 3849.0 15 3954.0 16 4123.0 17 4427.0 18 4855.0 19 6078.0 20 7515.0 21 9698.0 22 12266.0 23 14870.0 24 16793.0 25 19393.0 26 21468.0 27 24138.0 28 27710.0 29 32786.0 30 40435.0 31 50715.0 32 64194.0 33 82026.0 34 106906.0 35 142003.0 36 194577.0 37 303057.0 38 875235.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33583293937369 18.372511941052768 12.564414666529009 25.727240453044537 2 14.688253919535274 19.580953326668187 41.60265943881869 24.12813331497785 3 18.86283030466608 23.964414250548156 29.362485929357472 27.81026951542828 4 12.37261322579262 16.369553099731675 37.80420158016244 33.45363209431327 5 13.168841673467323 37.825122592386634 35.9025525937588 13.10348314038724 6 32.68008010880935 36.71877890925303 17.747900934392185 12.853240047545428 7 28.6606437754683 31.214491040122024 21.763500737819214 18.361364446590464 8 25.25260001218491 37.175311561451444 20.209264595381917 17.362823830981718 9 26.539998835701716 14.948190982825698 19.932786594504243 38.57902358696834 10 15.07823330637708 28.760126646256936 34.52956222562384 21.632077821742143 11 34.717036987564306 22.021371314747867 24.57944882603981 18.68214287164801 12 23.629847211498152 24.318133306016097 31.60222370742155 20.449795775064207 13 29.192152488020874 21.716131046987776 25.5155153257898 23.576201139201547 14 20.846453343611206 22.10362750889874 27.265100103215012 29.78481904427504 15 23.106369861684474 31.162447066002148 22.886261915367886 22.844921156945496 16 22.304370527516674 27.846334983670374 27.772415047355242 22.076879441457713 17 21.890821852958844 28.674393007212025 27.694964039166326 21.739821100662805 18 21.83576149129368 27.27743692028632 29.699813552156616 21.186988036263383 19 23.783479584155447 26.826991986742655 27.260780168857874 22.128748260244027 20 23.917708428762065 27.26563793928995 27.693820550508896 21.122833081439087 21 22.873536866276257 26.721822264905732 26.97234216930109 23.43229869951692 22 23.414516594138902 28.055992667648344 26.64394636480435 21.885544373408404 23 22.265671186462093 27.889526608366328 27.498621316478044 22.34618088869353 24 23.29604632566233 26.807971609647087 28.010834111023463 21.885147953667115 25 22.899935195163646 27.1224035193178 27.614802940948174 22.362858344570384 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 142.0 1 142.0 2 307.0 3 472.0 4 472.0 5 472.0 6 884.5 7 1297.0 8 1297.0 9 1297.0 10 1946.0 11 2595.0 12 2595.0 13 2595.0 14 5017.0 15 7439.0 16 7439.0 17 7439.0 18 14419.5 19 21400.0 20 21400.0 21 21400.0 22 35579.0 23 49758.0 24 49758.0 25 49758.0 26 73220.0 27 96682.0 28 96682.0 29 96682.0 30 128233.0 31 159784.0 32 159784.0 33 159784.0 34 183775.0 35 207766.0 36 207766.0 37 207766.0 38 226605.0 39 245444.0 40 245444.0 41 245444.0 42 259465.5 43 273487.0 44 273487.0 45 273487.0 46 286217.0 47 298947.0 48 298947.0 49 298947.0 50 287939.0 51 276931.0 52 276931.0 53 276931.0 54 247212.5 55 217494.0 56 217494.0 57 217494.0 58 187860.0 59 158226.0 60 158226.0 61 158226.0 62 129686.0 63 101146.0 64 101146.0 65 101146.0 66 78355.5 67 55565.0 68 55565.0 69 55565.0 70 40762.5 71 25960.0 72 25960.0 73 25960.0 74 18163.0 75 10366.0 76 10366.0 77 10366.0 78 7061.0 79 3756.0 80 3756.0 81 3756.0 82 2482.0 83 1208.0 84 1208.0 85 1208.0 86 794.0 87 380.0 88 380.0 89 380.0 90 232.5 91 85.0 92 85.0 93 85.0 94 53.0 95 21.0 96 21.0 97 21.0 98 18.5 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3618083106272562 2 0.03889247583996694 3 0.03433546880999401 4 0.0348317765063277 5 0.04038139892896799 6 0.017190293845739446 7 0.017009818319799926 8 0.023100867320258782 9 0.019852307853347392 10 0.01944623791998347 11 0.018318265882861458 12 0.019310881275528827 13 0.019356000157013706 14 0.027251804416867784 15 0.02869560862438396 16 0.020574209957105478 17 0.02183753863868213 18 0.10571353931907487 19 0.024319077120350554 20 0.022108251927591416 21 0.02594335685380625 22 0.019581594564438108 23 0.021882657520167013 24 0.018182909238406816 25 0.021792419757197253 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2216367.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.16812595076341 #Duplication Level Percentage of deduplicated Percentage of total 1 81.57424965680433 56.42337974600859 2 12.243383563603306 16.9370379278164 3 3.278569321780478 6.803174873616633 4 1.2395153412281554 3.4293981295989013 5 0.5427643793174423 1.8770997475108384 6 0.3088956283245482 1.2819439035355533 7 0.18741652245450574 0.9074274741271131 8 0.12611901517940727 0.6978732741372387 9 0.08273255297099318 0.5150210079713275 >10 0.3758335136626871 4.512947952703329 >50 0.02108830668741541 1.0024000569441671 >100 0.015444637820713447 2.149045901745437 >500 0.0024186840350597 1.1707996032501686 >1k 0.001503506292063854 2.06149170892783 >5k 6.536983878538496E-5 0.230958692106318 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5108 0.23046724662476925 No Hit CTGTAGGACGTGGAATATGGCAAGA 4687 0.21147219751963459 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4238 0.19121381973292328 No Hit CTTTAGGACGTGAAATATGGCGAGG 3882 0.17515149792430587 No Hit GTACATGGGAAGCAGTGGTATCAAC 3395 0.1531786026411691 No Hit CCCATGTACTCTGCGTTGATACCAC 2807 0.1266487003280594 No Hit GTATCAACGCAGAGTACTTTTTTTT 2607 0.1176249240310833 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.511888148488044E-5 2 0.0 0.0 0.0 0.0 4.511888148488044E-5 3 0.0 0.0 0.0 0.0 4.511888148488044E-5 4 0.0 0.0 0.0 0.0 4.511888148488044E-5 5 0.0 0.0 0.0 0.0 4.511888148488044E-5 6 0.0 0.0 0.0 0.0 9.023776296976088E-5 7 0.0 0.0 0.0 0.0 9.023776296976088E-5 8 0.0 0.0 0.0 0.0 1.3535664445464131E-4 9 0.0 0.0 0.0 0.0 1.3535664445464131E-4 10 0.0 0.0 0.0 0.0 1.3535664445464131E-4 11 0.0 0.0 0.0 0.0 1.3535664445464131E-4 12 0.0 0.0 0.0 0.0 1.8047552593952176E-4 13 0.0 0.0 0.0 0.0 1.8047552593952176E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACAC 50 0.0015028826 13.296493 3 TAGGACC 615 0.0 11.73674 4 CTACACG 95 1.3647916E-5 10.997099 4 GGACCGC 115 8.89233E-7 10.732421 6 AATCCCG 160 5.493348E-10 10.681305 19 TTGCGAG 90 9.495895E-5 10.558015 18 TGTAGGA 2405 0.0 10.544234 2 GTCCTAT 190 9.094947E-12 10.532658 1 TTAGGAC 1395 0.0 10.484689 3 GTCTAGA 220 0.0 10.395869 1 GTCCTAC 2105 0.0 10.367062 1 GGACCTG 1340 0.0 10.273429 6 GCCGGTT 130 3.870191E-7 10.225532 11 AGGACGT 2350 0.0 10.224731 5 GGACGTG 2355 0.0 10.19957 6 AGGACCT 1370 0.0 10.121189 5 TCCAACG 160 6.151822E-9 10.096102 18 CTGTAGG 2345 0.0 10.0375185 1 GGCGAGG 1015 0.0 10.008974 19 GACGTGG 1230 0.0 9.880002 7 >>END_MODULE