FastQCFastQC Report
Thu 26 May 2016
SRR1512960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512960_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2216367
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA49150.22175930249818737No Hit
GTCCTACAGTGGACATTTCTAAATT45920.20718590377857096No Hit
CTTTAGGACGTGAAATATGGCGAGG41450.1870177637548294No Hit
GTCCTAAAGTGTGTATTTCTCATTT41140.1856190784287981No Hit
CCCATGTACTCTGCGTTGATACCAC33420.15078730192247042No Hit
GTACATGGGAAGCAGTGGTATCAAC32190.14523767949983013No Hit
GTATCAACGCAGAGTACTTTTTTTT30250.13648461649176333No Hit
CTGAAGGACCTGGAATATGGCGAGA23370.10544282603016557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC957.396011E-812.9994910
TAGGACC6850.012.6387784
GTTCGGA550.003050380212.1008191
GACGTGG13350.011.3187647
AGGACGT25350.011.1088225
AAGCGAC600.005860948511.0874057
TGGACCG951.339078E-511.01598555
GTCCTAC21350.010.99963951
GGACGTG25100.010.9509236
GTAGGAC24100.010.7699873
TCCAACG1502.1664164E-910.75677118
TGTAGGA25400.010.742322
CTGTAGG24750.010.6026221
CTAGATA909.379222E-510.5708964
AGGACCT15900.010.5310055
GGCGAGG11100.010.51735219
TAGATAG1909.094947E-1210.5152595
GTATAAG1909.094947E-1210.5086051
CCACCTT6600.010.49957713
TTAGGAC13950.010.4958123
Produced by FastQC (version 0.11.5)