##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512960_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2216367
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.40262375319611	31.0	31.0	34.0	27.0	34.0
2	30.558340744109618	31.0	31.0	34.0	27.0	34.0
3	30.650781662062286	31.0	31.0	34.0	27.0	34.0
4	34.128250871809584	37.0	35.0	37.0	30.0	37.0
5	33.758072106289255	37.0	35.0	37.0	28.0	37.0
6	33.615653003315785	37.0	35.0	37.0	28.0	37.0
7	33.483318872731815	37.0	35.0	37.0	28.0	37.0
8	33.36458537778265	37.0	35.0	37.0	27.0	37.0
9	34.825136811728385	39.0	35.0	39.0	27.0	39.0
10	34.71543837279656	39.0	35.0	39.0	27.0	39.0
11	34.73205114495929	39.0	35.0	39.0	27.0	39.0
12	34.5848665857234	39.0	35.0	39.0	27.0	39.0
13	34.565735277596175	39.0	35.0	39.0	27.0	39.0
14	35.62616434913532	40.0	36.0	41.0	27.0	41.0
15	35.63949066197069	40.0	36.0	41.0	27.0	41.0
16	35.61507277449989	40.0	36.0	41.0	27.0	41.0
17	35.587387377631956	40.0	36.0	41.0	27.0	41.0
18	35.515635271595364	40.0	36.0	41.0	26.0	41.0
19	35.49097825405269	40.0	36.0	41.0	26.0	41.0
20	35.46357394781641	40.0	36.0	41.0	26.0	41.0
21	35.40990594066777	40.0	36.0	41.0	25.0	41.0
22	35.36042767285382	40.0	36.0	41.0	25.0	41.0
23	35.25076848734889	40.0	36.0	41.0	25.0	41.0
24	35.23186548076198	40.0	36.0	41.0	25.0	41.0
25	35.13507194431247	40.0	36.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7860150856928136
1101	2	0.8529446423784286
1101	3	0.8148686920064812
1101	4	0.5480095501986639
1101	5	0.924466958652431
1101	6	0.8968363770603034
1101	7	0.907243805787374
1101	8	0.9909621930090466
1101	9	0.9054675437730495
1101	10	1.2373516834934435
1101	11	1.179296685969753
1101	12	1.1819755144675952
1101	13	1.2582939543085345
1101	14	1.380594417979502
1101	15	1.36045254863744
1101	16	1.4789350625719209
1101	17	1.4160801337540434
1101	18	0.9048451357972809
1101	19	1.3116523295308795
1101	20	1.2928370181003714
1101	21	1.3623022306164927
1101	22	1.168710563858376
1101	23	1.1677977480229984
1101	24	1.2168404079152708
1101	25	1.33947518246827
1102	1	0.6359834756332319
1102	2	0.5593925261031494
1102	3	0.5800949441831698
1102	4	0.5099645031548334
1102	5	0.7494767241091651
1102	6	0.6538600896675746
1102	7	0.6154786925995523
1102	8	0.6260139743544784
1102	9	0.7936561283760071
1102	10	0.917429281349186
1102	11	0.7391422825081548
1102	12	0.8897610748076374
1102	13	0.9531023373069942
1102	14	1.1392673781181415
1102	15	0.963623821870037
1102	16	0.9402469786318051
1102	17	0.9913146512640978
1102	18	0.9524079282613869
1102	19	1.0145960174398496
1102	20	0.9672393712601419
1102	21	1.0617865754214009
1102	22	1.0447885610364764
1102	23	1.067610645602322
1102	24	1.0329623198948923
1102	25	1.1262723915460313
1103	1	0.5288579495333927
1103	2	0.4886752940807604
1103	3	0.4875220630677468
1103	4	0.4543201860848143
1103	5	0.553441758863805
1103	6	0.6689922300496036
1103	7	0.5985037534047635
1103	8	0.6596366042273516
1103	9	0.7852487898366434
1103	10	0.8185096238773468
1103	11	0.8285575085471351
1103	12	0.7894895775068775
1103	13	0.8997965195163147
1103	14	0.9537626425407169
1103	15	0.756075588274939
1103	16	0.9411251874410169
1103	17	1.0182557561002596
1103	18	1.136036588438202
1103	19	1.1506747612262487
1103	20	1.0821335150720728
1103	21	0.8369288263687693
1103	22	1.099272766597167
1103	23	1.143637562396286
1103	24	1.0427175326594451
1103	25	1.058742125584196
1104	1	0.6506186691395364
1104	2	0.6799984139179074
1104	3	0.6578325159321032
1104	4	0.6034908656106452
1104	5	0.6184181107455728
1104	6	0.8109586390042409
1104	7	0.7659800299094996
1104	8	0.6993818780030665
1104	9	0.9099927507429513
1104	10	0.9575681263346354
1104	11	0.9193715019360837
1104	12	0.8476978743516739
1104	13	0.9067882986481806
1104	14	1.1433047530485965
1104	15	1.0829300722623643
1104	16	1.0752218120955774
1104	17	1.0236311307578845
1104	18	1.139026924047144
1104	19	1.169069459345252
1104	20	1.1569896209536168
1104	21	1.0042081513486707
1104	22	1.1414184504239273
1104	23	1.006951599491245
1104	24	1.0249547347586514
1104	25	1.1343055509428197
1105	1	0.28474277366540335
1105	2	0.3132573720870617
1105	3	0.2706439054986127
1105	4	0.28245869617840214
1105	5	0.21917112584082332
1105	6	0.30497827719113957
1105	7	0.32408872031635383
1105	8	0.38722038490826804
1105	9	0.5342608083321991
1105	10	0.47986863644324984
1105	11	0.4867714027134653
1105	12	0.3845090659411241
1105	13	0.5256272869519876
1105	14	0.6437289657093999
1105	15	0.6592563304241139
1105	16	0.5789572077344971
1105	17	0.36430926236948835
1105	18	0.6174233466820098
1105	19	0.6145962563925167
1105	20	0.6745727090311675
1105	21	0.5590787297008433
1105	22	0.7441896944572335
1105	23	0.7999169262511998
1105	24	0.6808503794473566
1105	25	0.7446064442975597
1106	1	0.3433922106020084
1106	2	0.34951956883490354
1106	3	0.3419531039268797
1106	4	0.30986436978476917
1106	5	0.3743266468080364
1106	6	0.2986175727067888
1106	7	0.4194711763321166
1106	8	0.4142131026151006
1106	9	0.4373110268601792
1106	10	0.4675702113636788
1106	11	0.2869650251093532
1106	12	0.45767229169221224
1106	13	0.29661118397341113
1106	14	0.4909121034311781
1106	15	0.5496706244848824
1106	16	0.5641095361843682
1106	17	0.4373710574716867
1106	18	0.5784716760025006
1106	19	0.5935627947995528
1106	20	0.6490212271408993
1106	21	0.5423393847685816
1106	22	0.6538685785030935
1106	23	0.7726056143830249
1106	24	0.7365417238456757
1106	25	0.801416813699106
1107	1	0.5812517971906246
1107	2	0.534434451412725
1107	3	0.5487156271437286
1107	4	0.5481498741911679
1107	5	0.5475358118147682
1107	6	0.5973479685245664
1107	7	0.6652758123365103
1107	8	0.7685079726407551
1107	9	0.6719102517485425
1107	10	0.8130155282029321
1107	11	0.8309173595489057
1107	12	0.7222782270725787
1107	13	0.7568322888871961
1107	14	0.9620856491006577
1107	15	0.8630872643819814
1107	16	0.8600765288599845
1107	17	0.8709459238783523
1107	18	1.027025441676173
1107	19	1.0984379043615249
1107	20	0.9840719771759652
1107	21	0.9658019692437776
1107	22	1.069121221422776
1107	23	1.0480294213926058
1107	24	1.0539325623991402
1107	25	1.063286210768993
1108	1	0.4898868807993608
1108	2	0.41495731529848
1108	3	0.4474113156949464
1108	4	0.5669044894738278
1108	5	0.545645356433738
1108	6	0.6382255968385238
1108	7	0.5489832313725387
1108	8	0.5756724611124824
1108	9	0.5643856356939381
1108	10	0.6479486643586014
1108	11	0.7308562513049424
1108	12	0.7352352462590446
1108	13	0.8257236613773813
1108	14	0.7782802723843503
1108	15	0.8245673041088111
1108	16	0.7185017066216943
1108	17	0.7870166799747338
1108	18	0.8399906010762805
1108	19	0.7708381620648268
1108	20	0.7360886220517813
1108	21	0.795018668689778
1108	22	0.8067020955781459
1108	23	0.7912798815250639
1108	24	0.8578035010110838
1108	25	0.9175892523120837
1109	1	0.310088824716523
1109	2	0.2595146231905794
1109	3	0.247556598740303
1109	4	0.3528400159469598
1109	5	0.4237385515617973
1109	6	0.027257380469905
1109	7	0.3400361001680494
1109	8	0.4316156801800446
1109	9	0.5737828006170602
1109	10	0.44076032456958814
1109	11	0.5061833560089468
1109	12	0.4698402540460407
1109	13	0.3846085595412774
1109	14	0.5086783169452431
1109	15	0.5610712547329086
1109	16	0.4343699943005248
1109	17	0.40737068455349856
1109	18	0.4670965637809985
1109	19	0.7704808848656484
1109	20	0.7777145625065529
1109	21	0.744549633439874
1109	22	0.7490671834959315
1109	23	0.564120569298197
1109	24	0.221595683805333
1109	25	0.3740551708267361
1110	1	0.19731902004749458
1110	2	0.21250352409496998
1110	3	0.21246163307252175
1110	4	0.2852295862305496
1110	5	0.20382063194892908
1110	6	0.16303385496516398
1110	7	0.24870589608222815
1110	8	0.3602323502948508
1110	9	0.4130442434261212
1110	10	0.29113162313883123
1110	11	0.2332660687994874
1110	12	0.42229823932517974
1110	13	-0.036289169626705586
1110	14	0.305822083809943
1110	15	0.4041058422596464
1110	16	0.3582196730366576
1110	17	0.28704774308040015
1110	18	0.21268380145952648
1110	19	0.28436857138919436
1110	20	0.25780852796940223
1110	21	0.3544831601624665
1110	22	0.30255662705429387
1110	23	0.3975700633510115
1110	24	0.30913538020278253
1110	25	0.31108114338542237
1111	1	-0.305625986700818
1111	2	-0.1158302862570686
1111	3	-0.03794233172015993
1111	4	-0.07525545172746462
1111	5	0.07781097015185168
1111	6	-0.07653678149787879
1111	7	0.1428569462239011
1111	8	0.20293643257424065
1111	9	-0.10072174920977517
1111	10	-0.04872802661601838
1111	11	0.04425190968339621
1111	12	-0.009702577277472813
1111	13	0.11633680167344806
1111	14	0.3355064909023753
1111	15	0.33323720787413436
1111	16	0.06695355197264519
1111	17	0.2638376140051406
1111	18	0.16475209452647022
1111	19	0.27504302246742185
1111	20	0.06353482064005789
1111	21	0.002596134203976419
1111	22	-0.034273286755428956
1111	23	-0.04614456471116313
1111	24	0.013307559270934632
1111	25	0.10608652402299867
1112	1	0.18130102024682415
1112	2	0.03788326271363118
1112	3	0.16825354037472806
1112	4	-0.008255944164275775
1112	5	0.1773641640327881
1112	6	0.10482415059937011
1112	7	0.0997523328403247
1112	8	0.11120669474331635
1112	9	-0.13897860318084554
1112	10	0.23931974243111398
1112	11	0.09905323551598855
1112	12	0.29652420593257034
1112	13	0.21298187516428868
1112	14	0.3059760053168574
1112	15	0.2881598364161988
1112	16	0.04312054693051692
1112	17	0.22022417155066876
1112	18	0.23032346842518336
1112	19	0.25799373596554176
1112	20	0.5936550929790272
1112	21	0.5774816560264213
1112	22	0.3753100287359885
1112	23	0.520438111147655
1112	24	0.5010956615693303
1112	25	0.5993871085240841
1113	1	-0.2627930164504235
1113	2	-0.6344217831579293
1113	3	-0.22853545045095913
1113	4	-0.25768254966886417
1113	5	-0.1382895056919793
1113	6	-0.2211506200428346
1113	7	-0.08086693321472183
1113	8	0.011436052541483832
1113	9	-0.040928144465254945
1113	10	-0.3816664961009977
1113	11	-0.1819100672363092
1113	12	-0.11517304177385057
1113	13	-0.0573736294228695
1113	14	-0.09310656349047974
1113	15	-0.017459429638847723
1113	16	-0.4224757833447086
1113	17	-0.18774601193557316
1113	18	0.004119339984811177
1113	19	-0.12188011724546755
1113	20	0.14698078105242018
1113	21	-0.12234632562495307
1113	22	-0.35032070520896497
1113	23	-0.11574308151289614
1113	24	-0.464213971458193
1113	25	-0.4267023410172044
1114	1	-0.6718129325513154
1114	2	-0.5429764580290062
1114	3	-0.5074436755316505
1114	4	-0.275555829154122
1114	5	-0.504154115690973
1114	6	-0.7914224176461673
1114	7	-0.6901098471055107
1114	8	-1.0789257524288658
1114	9	-0.8171234285191247
1114	10	-0.8937301399087332
1114	11	-0.8252214254160748
1114	12	-0.7882503980244167
1114	13	-0.786535658583503
1114	14	-1.6952371993853035
1114	15	-1.1209983767394789
1114	16	-1.0985211434536808
1114	17	-1.1126894674870655
1114	18	-1.2326952366241386
1114	19	-1.1437606988152211
1114	20	-0.51666918794254
1114	21	-0.6558728442094264
1114	22	-0.819814859301367
1114	23	-0.7738252276446858
1114	24	-1.3696610204143553
1114	25	-1.2595137892970811
1115	1	-1.528384604365165
1115	2	-1.160920966012739
1115	3	-0.9401674326647864
1115	4	-2.111736903089465
1115	5	-1.3647509818064023
1115	6	-1.3569608805211288
1115	7	-1.065406340572217
1115	8	-1.061516043648183
1115	9	-1.5617454488412221
1115	10	-1.349087971897049
1115	11	-1.246068069422435
1115	12	-1.7395870452016169
1115	13	-1.2335622705740832
1115	14	-2.1539386122963293
1115	15	-2.1605785630404526
1115	16	-1.5513416362267378
1115	17	-1.5028083789504976
1115	18	-1.4275392657744703
1115	19	-1.2066614181512207
1115	20	-1.4016098424718066
1115	21	-1.4042574372077254
1115	22	-1.6554955822392259
1115	23	-1.8325470555446586
1115	24	-1.4715963012240323
1115	25	-1.3108260788197157
1116	1	-0.6437113510974903
1116	2	-0.7141793362886055
1116	3	-0.5148596690730756
1116	4	-0.627665229786821
1116	5	-0.5902879814668083
1116	6	-1.2224962004823823
1116	7	-0.5728764770930184
1116	8	-0.7971320320344404
1116	9	-0.8523668216317191
1116	10	-0.7904116986636538
1116	11	-0.8101189830406526
1116	12	-1.3496898164353226
1116	13	-0.9976211381552105
1116	14	-1.34070131287789
1116	15	-1.306960533930848
1116	16	-1.5385814793256927
1116	17	-1.458308020387122
1116	18	-2.292982900833948
1116	19	-1.729100087302406
1116	20	-1.5170407369020822
1116	21	-1.502055935861506
1116	22	-2.7380901046101016
1116	23	-2.6222417360898547
1116	24	-1.841175679471803
1116	25	-2.1909398538292564
1201	1	0.7872570466793363
1201	2	0.7718528982554105
1201	3	0.7281335528367094
1201	4	0.7079125849669694
1201	5	0.7656465904565621
1201	6	0.873579035640347
1201	7	0.8707030950433605
1201	8	0.8473693891542808
1201	9	0.9125601164055013
1201	10	0.8055642288254248
1201	11	1.0161393154038265
1201	12	0.7881702734404215
1201	13	0.8868414230622861
1201	14	0.9368825493587494
1201	15	1.1161054478046992
1201	16	0.9891618531973094
1201	17	0.9521544732890561
1201	18	1.0816539930307556
1201	19	0.8893326744563907
1201	20	1.0298677309558144
1201	21	0.9491344635243806
1201	22	1.1424048538720228
1201	23	0.934522367514603
1201	24	0.9961655279036776
1201	25	0.8898770952149064
1202	1	0.5855714039414828
1202	2	0.613182481402621
1202	3	0.6116690798872817
1202	4	0.6149432571562699
1202	5	0.7026320622886786
1202	6	0.786889861911483
1202	7	0.7297140380990612
1202	8	0.709315553191793
1202	9	1.0611667061256753
1202	10	0.9326195764210397
1202	11	0.9989770465849404
1202	12	0.8654246146970408
1202	13	0.8626431398837155
1202	14	0.9397479986226003
1202	15	1.0402317646912351
1202	16	0.8851940370197298
1202	17	1.0220540186275144
1202	18	0.9181423107708113
1202	19	1.2355988689486352
1202	20	1.1972387160109363
1202	21	1.100344814849457
1202	22	1.1251428237371073
1202	23	1.0978137180336418
1202	24	0.9999404576314035
1202	25	1.1508116917485793
1203	1	0.6173288412600719
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2211	9	-0.6625087833586605
2211	10	-0.8522249698672937
2211	11	-0.6408191472844109
2211	12	-0.6323325366878052
2211	13	-0.6266775606706645
2211	14	-1.6667552627022317
2211	15	-1.0168351615619855
2211	16	-0.7808834468097317
2211	17	-0.6326523848527046
2211	18	-0.7332203590011304
2211	19	-0.6901014258583942
2211	20	-0.8269158201984794
2211	21	-0.8091895640992988
2211	22	-0.7441790495266147
2211	23	-0.7533671145866307
2211	24	-0.8633823689206821
2211	25	-0.9820372073411221
2212	1	-0.2658441953716668
2212	2	-0.24292174144466117
2212	3	-0.2414981744316549
2212	4	-0.10268663626560937
2212	5	-0.21772289286150226
2212	6	-0.19352356403494753
2212	7	-0.258439582737239
2212	8	-0.5325033354727324
2212	9	-0.5827906679615538
2212	10	-0.6823234311925432
2212	11	-0.36455087884615267
2212	12	-0.381234116516211
2212	13	-0.3322427214553727
2212	14	-0.3906904039007273
2212	15	-0.27512652386651126
2212	16	-0.3848499265932901
2212	17	-0.4625205158735639
2212	18	-0.24818776222051753
2212	19	-0.3353556700114453
2212	20	-0.5028078174078345
2212	21	-0.2287562210550078
2212	22	-0.40025784201023384
2212	23	-0.8106663323582382
2212	24	-0.6464402574652155
2212	25	-0.8019292383843108
2213	1	-0.39692448855840823
2213	2	-0.553512524484514
2213	3	-0.3864337948567389
2213	4	-0.30506338906567976
2213	5	-0.32735876120183605
2213	6	-0.2029614921297238
2213	7	-0.7934176819229393
2213	8	-0.4034695680427234
2213	9	-0.6086282393719245
2213	10	-0.46676944348051563
2213	11	-0.3395045784669293
2213	12	-0.5858798328737151
2213	13	-0.5213974088175704
2213	14	-0.5306243584920622
2213	15	-0.7266284689723861
2213	16	-0.8435384744762118
2213	17	-0.7928215418311737
2213	18	-0.7986527258171492
2213	19	-1.060916419048695
2213	20	-0.43604806003162366
2213	21	-1.1857255305179208
2213	22	-0.8430827530841114
2213	23	-0.8705866173138048
2213	24	-0.9489096110237298
2213	25	-0.851188922267859
2214	1	-1.147388783437993
2214	2	-1.0185509512170938
2214	3	-0.8648766680147517
2214	4	-1.5039504320306776
2214	5	-1.429444204966444
2214	6	-2.7879453515513646
2214	7	-1.4401807868569563
2214	8	-2.0165167606626255
2214	9	-1.535863945843019
2214	10	-1.8726070652101896
2214	11	-1.669572652538001
2214	12	-1.632983308163098
2214	13	-1.8969532119970651
2214	14	-1.882295820058573
2214	15	-2.187417331297979
2214	16	-1.714817362059442
2214	17	-1.9271584274424214
2214	18	-1.9977288634240224
2214	19	-3.7411182100450873
2214	20	-2.2128529008544575
2214	21	-2.6776859692174924
2214	22	-2.4866383401554373
2214	23	-2.480015348893957
2214	24	-1.654072570084736
2214	25	-1.5354684041485385
2215	1	-1.602063110522593
2215	2	-1.3526367878561842
2215	3	-1.4328563050781362
2215	4	-1.2415607444148478
2215	5	-1.9645450338373323
2215	6	-1.797121291424709
2215	7	-2.035538400087347
2215	8	-2.7332810884570087
2215	9	-2.3240247357546053
2215	10	-1.7622710215505393
2215	11	-2.272994209490342
2215	12	-3.0366655138587255
2215	13	-2.492155066901283
2215	14	-2.5145129511085074
2215	15	-3.4700930621791386
2215	16	-3.063276340442556
2215	17	-2.8123605978065385
2215	18	-3.4163086629492554
2215	19	-4.050667181251484
2215	20	-3.547918634811346
2215	21	-2.765297070251407
2215	22	-3.6808039092845313
2215	23	-3.4881601292155686
2215	24	-2.479759286856954
2215	25	-2.6598148106659423
2216	1	-1.064080535750204
2216	2	-1.0888522354446586
2216	3	-1.4107194478372556
2216	4	-1.036161582071415
2216	5	-1.1015924226594507
2216	6	-1.4341105212911955
2216	7	-2.6298351854055433
2216	8	-1.7954719514001525
2216	9	-1.835643034673872
2216	10	-1.8115541160494573
2216	11	-2.3187772913350813
2216	12	-1.7912341344707627
2216	13	-2.22131735837754
2216	14	-2.3264987073601304
2216	15	-2.3446223224190135
2216	16	-2.1544469561916983
2216	17	-2.2861081983306093
2216	18	-2.0986190551166004
2216	19	-2.066634496253627
2216	20	-2.6661166558313667
2216	21	-2.394078009935882
2216	22	-2.690027467114227
2216	23	-1.9299710046721046
2216	24	-2.2066911740044475
2216	25	-2.055266378083317
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58990.0
3	2.0
4	981.0
5	8984.0
6	10049.0
7	7828.0
8	6176.0
9	5384.0
10	4778.0
11	4362.0
12	4079.0
13	3692.0
14	3663.0
15	3635.0
16	3709.0
17	3995.0
18	4440.0
19	5223.0
20	6516.0
21	8403.0
22	10627.0
23	12862.0
24	15522.0
25	17824.0
26	20116.0
27	22186.0
28	24954.0
29	29993.0
30	37948.0
31	48122.0
32	61218.0
33	79296.0
34	103253.0
35	135783.0
36	185786.0
37	290312.0
38	965676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.6657053075243	19.06168819000322	13.543875049501642	27.728731452970845
2	13.608955929621668	19.881301085922708	40.29541176449356	26.214331219962062
3	17.12487146758637	23.833056528995424	29.89716955720781	29.144902446210395
4	11.487631540823294	16.780585596705777	37.120135466118356	34.61164739635258
5	12.818264526805084	38.03439160008759	35.9582984352883	13.189045437819022
6	32.87607274868398	36.87817151155178	17.385595298193206	12.86016044157104
7	28.39768786753306	31.35203421891058	21.83811552267382	18.41216239088254
8	25.2227539503386	37.404559819413095	19.930293453724605	17.4423927765237
9	26.122631004273185	14.742378838002152	19.79540886405112	39.33958129367355
10	14.731495018501933	28.894134076572215	34.58558425504258	21.78878664988327
11	34.536135967397854	21.882907337287076	24.61494313033999	18.966013564975075
12	23.396807060925752	24.358795021975148	31.587174095499382	20.657223821599718
13	28.980863698692254	21.547075499749806	25.683370039332043	23.788690762225894
14	20.635932644509328	22.022785428119054	27.229710126290417	30.1115718010812
15	22.94183975645226	31.26152007973869	22.83363842979029	22.96300173401876
16	22.243105026869625	27.84477573146348	27.743876437171917	22.168242804494984
17	21.828929055596973	28.738882500645342	27.641438419839375	21.790750023918307
18	21.840784100337988	27.328322389659753	29.521839746357635	21.309053763644627
19	23.60009926454	26.901818345891805	27.250597843252265	22.24748454631593
20	23.8287302539853	27.212117657144663	27.61378351915606	21.34536856971398
21	22.742959756177346	26.705844930776294	27.05285456141024	23.498340751636125
22	23.29831498645077	27.963347142750145	26.724018910268267	22.014318960530815
23	22.10008541004265	27.77419082669973	27.550310936771755	22.575412826485866
24	23.269702174775205	26.82881490294703	27.926782883881597	21.97470003839617
25	22.75912572773677	27.08351663524026	27.663134185117556	22.494223451905416
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	105.0
1	105.0
2	224.5
3	344.0
4	344.0
5	344.0
6	826.5
7	1309.0
8	1309.0
9	1309.0
10	1936.0
11	2563.0
12	2563.0
13	2563.0
14	4983.0
15	7403.0
16	7403.0
17	7403.0
18	14290.5
19	21178.0
20	21178.0
21	21178.0
22	35780.5
23	50383.0
24	50383.0
25	50383.0
26	73804.0
27	97225.0
28	97225.0
29	97225.0
30	128070.0
31	158915.0
32	158915.0
33	158915.0
34	182317.0
35	205719.0
36	205719.0
37	205719.0
38	224927.5
39	244136.0
40	244136.0
41	244136.0
42	259758.0
43	275380.0
44	275380.0
45	275380.0
46	288048.0
47	300716.0
48	300716.0
49	300716.0
50	289698.0
51	278680.0
52	278680.0
53	278680.0
54	248306.5
55	217933.0
56	217933.0
57	217933.0
58	188276.0
59	158619.0
60	158619.0
61	158619.0
62	129416.5
63	100214.0
64	100214.0
65	100214.0
66	77503.5
67	54793.0
68	54793.0
69	54793.0
70	40176.0
71	25559.0
72	25559.0
73	25559.0
74	17770.5
75	9982.0
76	9982.0
77	9982.0
78	6812.0
79	3642.0
80	3642.0
81	3642.0
82	2376.0
83	1110.0
84	1110.0
85	1110.0
86	742.0
87	374.0
88	374.0
89	374.0
90	226.5
91	79.0
92	79.0
93	79.0
94	42.5
95	6.0
96	6.0
97	6.0
98	3.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08094327338387551
2	1.3535664445464131E-4
3	0.0
4	0.027522517705777065
5	0.07137807050908086
6	0.07801054608735827
7	0.03383916111366033
8	0.061677510989831555
9	0.041689846492029524
10	0.10260033649661811
11	0.003203440585426511
12	0.010241986097067859
13	0.004872839200367088
14	0.005684979067094935
15	0.0057752168300646955
16	0.01335518891952461
17	0.022153370809076294
18	8.121398667278479E-4
19	0.0030229650594869894
20	0.004376531504033402
21	9.023776296976087E-5
22	2.255944074244022E-4
23	0.0
24	0.0
25	0.0035192727558206738
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2216367.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.32150512247193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.86940937777983	55.251197670552656
2	12.65726733469356	17.295271100875254
3	3.4664489659692004	7.104990323557566
4	1.2575588229901111	3.4367324626691462
5	0.5892656391567217	2.012975769207133
6	0.31090630863077806	1.2744952174635928
7	0.1905653560068765	0.9113798362612651
8	0.12637453194731366	0.6907278585430706
9	0.08980942299362703	0.5522323457794803
>10	0.40141155699193604	4.695060820956233
>50	0.020739456332298567	0.9860682525334311
>100	0.01614506555371127	2.19159664136983
>500	0.0025117760686929596	1.2212641682909635
>1k	0.0015863848854892526	2.376007531940323
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGA	4915	0.22175930249818737	No Hit
GTCCTACAGTGGACATTTCTAAATT	4592	0.20718590377857096	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4145	0.1870177637548294	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4114	0.1856190784287981	No Hit
CCCATGTACTCTGCGTTGATACCAC	3342	0.15078730192247042	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3219	0.14523767949983013	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3025	0.13648461649176333	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2337	0.10544282603016557	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.023776296976088E-5
2	0.0	0.0	0.0	0.0	9.023776296976088E-5
3	0.0	0.0	0.0	0.0	9.023776296976088E-5
4	0.0	0.0	0.0	0.0	9.023776296976088E-5
5	0.0	0.0	0.0	0.0	9.023776296976088E-5
6	0.0	0.0	0.0	0.0	9.023776296976088E-5
7	0.0	0.0	0.0	0.0	9.023776296976088E-5
8	0.0	0.0	0.0	0.0	9.023776296976088E-5
9	0.0	0.0	0.0	0.0	9.023776296976088E-5
10	0.0	0.0	0.0	0.0	9.023776296976088E-5
11	0.0	0.0	0.0	0.0	9.023776296976088E-5
12	0.0	0.0	0.0	0.0	1.8047552593952176E-4
13	0.0	0.0	0.0	0.0	2.2559440742440218E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	95	7.396011E-8	12.99949	10
TAGGACC	685	0.0	12.638778	4
GTTCGGA	55	0.0030503802	12.100819	1
GACGTGG	1335	0.0	11.318764	7
AGGACGT	2535	0.0	11.108822	5
AAGCGAC	60	0.0058609485	11.087405	7
TGGACCG	95	1.339078E-5	11.0159855	5
GTCCTAC	2135	0.0	10.9996395	1
GGACGTG	2510	0.0	10.950923	6
GTAGGAC	2410	0.0	10.769987	3
TCCAACG	150	2.1664164E-9	10.756771	18
TGTAGGA	2540	0.0	10.74232	2
CTGTAGG	2475	0.0	10.602622	1
CTAGATA	90	9.379222E-5	10.570896	4
AGGACCT	1590	0.0	10.531005	5
GGCGAGG	1110	0.0	10.517352	19
TAGATAG	190	9.094947E-12	10.515259	5
GTATAAG	190	9.094947E-12	10.508605	1
CCACCTT	660	0.0	10.499577	13
TTAGGAC	1395	0.0	10.495812	3
>>END_MODULE