FastQCFastQC Report
Thu 26 May 2016
SRR1512959_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512959_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661340
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC39460.2375191110790085No Hit
CCCATGTACTCTGCGTTGATACCAC33790.20339003455042315No Hit
GTATCAACGCAGAGTACTTTTTTTT29920.18009558549122998No Hit
TATCAACGCAGAGTACTTTTTTTTT18590.1118976248088892No Hit
GGTATCAACGCAGAGTACTTTTTTT18000.10834627469392179No Hit
GTCCTACAGTGGACATTTCTAAATT17550.10563761782657374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG400.00528691614.2469055
TACACCG655.464048E-513.1509895
GGACCGT701.09689594E-412.2072196
AAAGCCG550.003073593812.0882825
TGGACCG658.0385606E-411.6897685
GTCTTAG1406.4574124E-1011.5719011
CCTAGAC909.5389434E-510.5529453
CCCAATA1002.4051988E-510.4474152
GGCGAGG3500.010.03704719
GGACGTG9750.010.0300926
AGGACGT10000.09.8778545
CTCGAAC1554.0505256E-89.80816318
TAGGACA3850.09.6212864
GACGTGG5750.09.57706457
GTATAAG1701.5808837E-89.5298011
ATAAGCC1201.7050419E-59.4976513
CTTAGAC1901.0550139E-99.4976513
CTTACAC1901.0550139E-99.4976513
GTTGGAC1106.853956E-59.497653
ATACTGC1502.6694215E-79.4945056
Produced by FastQC (version 0.11.5)