##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512959_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1661340
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.138592341122227	31.0	30.0	34.0	23.0	34.0
2	29.215176303465878	31.0	30.0	34.0	22.0	34.0
3	29.351159305139223	31.0	30.0	34.0	23.0	34.0
4	32.7656825213382	35.0	33.0	37.0	25.0	37.0
5	32.891350957660684	37.0	35.0	37.0	26.0	37.0
6	32.85100160111717	37.0	35.0	37.0	26.0	37.0
7	32.73881986829908	37.0	35.0	37.0	25.0	37.0
8	32.717204786497646	37.0	35.0	37.0	25.0	37.0
9	34.029846389059436	39.0	35.0	39.0	25.0	39.0
10	33.90887777336367	38.0	34.0	39.0	23.0	39.0
11	33.985037981388516	39.0	35.0	39.0	25.0	39.0
12	33.87568348441619	39.0	34.0	39.0	23.0	39.0
13	33.878262125753906	39.0	35.0	39.0	23.0	39.0
14	34.88744086099173	39.0	35.0	41.0	22.0	41.0
15	34.83032612228683	39.0	34.0	41.0	22.0	41.0
16	34.70914201788917	39.0	34.0	41.0	19.0	41.0
17	34.654917115099856	39.0	34.0	41.0	19.0	41.0
18	34.35348935196889	39.0	34.0	41.0	17.0	41.0
19	34.59656903463469	39.0	34.0	41.0	18.0	41.0
20	34.674443521494695	39.0	34.0	41.0	18.0	41.0
21	34.61340062840839	39.0	34.0	41.0	18.0	41.0
22	34.5319982664596	39.0	34.0	41.0	18.0	41.0
23	34.269835193277714	39.0	34.0	41.0	15.0	41.0
24	34.276115665667476	39.0	34.0	41.0	10.0	41.0
25	34.19269204377189	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.49261722139446107
1101	2	0.709179112469954
1101	3	0.6536963481682783
1101	4	0.581875291684014
1101	5	0.43426588072676253
1101	6	0.6452916039047736
1101	7	0.645907556742813
1101	8	0.5267107667607718
1101	9	0.4241805694575689
1101	10	0.5157530821997938
1101	11	0.8283714390763208
1101	12	0.9944461810110852
1101	13	0.8657936447575167
1101	14	0.7946336018305971
1101	15	0.5057793112664157
1101	16	0.9762012818056078
1101	17	0.8878094179574205
1101	18	1.220609923019019
1101	19	0.6634298872910023
1101	20	0.9687287857784881
1101	21	1.0057908057422864
1101	22	0.8198689227138374
1101	23	1.1228125216285463
1101	24	0.8134212628992046
1101	25	0.9925680409734454
1102	1	0.6776400278476018
1102	2	0.6983831435635395
1102	3	0.7836660102237758
1102	4	0.7289741088408519
1102	5	0.6832505633691639
1102	6	0.6016778472118531
1102	7	0.7412654023372625
1102	8	0.8083049989351991
1102	9	0.7504945902514706
1102	10	0.8588860828506952
1102	11	0.9299960715420355
1102	12	0.755062902917004
1102	13	0.7891722543245052
1102	14	0.9196223794097023
1102	15	0.9718723251847123
1102	16	0.9416006877654581
1102	17	0.84089595778579
1102	18	1.2211348829300377
1102	19	1.1131867971849374
1102	20	1.150771727748598
1102	21	0.9639149656193879
1102	22	1.3660423265738544
1102	23	1.531525609215798
1102	24	1.3312596126546836
1102	25	1.2282547783164262
1103	1	0.658320394734865
1103	2	0.7849242961663982
1103	3	0.8044074536966193
1103	4	0.8250496555969775
1103	5	0.3617908054756498
1103	6	0.723690930250342
1103	7	0.5637798694524534
1103	8	0.773269220705231
1103	9	0.8545327027741578
1103	10	0.8639365879012004
1103	11	0.9819227796393477
1103	12	1.1643629511916416
1103	13	1.1280545741959358
1103	14	1.2585972765969373
1103	15	1.2220298288358649
1103	16	1.221309145604586
1103	17	1.1857382097060807
1103	18	1.5960210708833742
1103	19	1.5248822453141386
1103	20	1.4276828535254182
1103	21	1.18745465776162
1103	22	1.2429997410085818
1103	23	1.5321366806072731
1103	24	1.4116022098303063
1103	25	1.2135806626894947
1104	1	0.4952855988839353
1104	2	0.5905305884249294
1104	3	0.5456534442872645
1104	4	0.6656954256984662
1104	5	0.5284450715174458
1104	6	0.4802376284327394
1104	7	0.8011952767751751
1104	8	0.7029002113790526
1104	9	0.7674517342551113
1104	10	0.7618764206742341
1104	11	0.8626508475463766
1104	12	0.8596824714386884
1104	13	0.8882670180717653
1104	14	0.9599409296697985
1104	15	1.0790515314416638
1104	16	0.8271338098425502
1104	17	0.8428512534999442
1104	18	1.2526190350637165
1104	19	1.039873239738398
1104	20	1.099008374465086
1104	21	1.0681657882298055
1104	22	1.0381879961045613
1104	23	1.3465507435207869
1104	24	1.2161151299203823
1104	25	1.0582477134730794
1105	1	0.5222779366025136
1105	2	0.7232917069886682
1105	3	0.6925962956311373
1105	4	0.7790087626102533
1105	5	0.39642890112028084
1105	6	0.24989858238961205
1105	7	0.6803661445481453
1105	8	0.6527387786227337
1105	9	0.6180363524091916
1105	10	0.8906152065890396
1105	11	0.7762028837926707
1105	12	0.6955335378366811
1105	13	0.7813378228691192
1105	14	0.8203878031972991
1105	15	0.9747197450294323
1105	16	0.9175769847076154
1105	17	0.796554788866878
1105	18	0.8749584792867822
1105	19	0.8871736185160017
1105	20	0.9709534387475998
1105	21	0.7803204394095786
1105	22	0.5344924630814063
1105	23	0.8166090131011146
1105	24	0.8279650959588523
1105	25	0.979648305437891
1106	1	0.455929093444972
1106	2	0.5419433577250103
1106	3	0.4976029059301901
1106	4	0.4996111701568964
1106	5	0.5757660736456529
1106	6	0.4654789573083207
1106	7	0.3375643961783936
1106	8	0.3366812744924843
1106	9	0.46519494516326887
1106	10	0.47241023435433505
1106	11	0.5556805060226537
1106	12	0.5975422195719062
1106	13	0.5747308504882227
1106	14	0.7363310040877948
1106	15	0.7347042439109828
1106	16	0.7892044080887715
1106	17	0.5923684071772257
1106	18	0.8435377822296175
1106	19	0.4469945269043052
1106	20	0.5940636745487353
1106	21	0.6017870664091021
1106	22	0.6234280874792688
1106	23	0.8581480418795948
1106	24	0.8233388350156687
1106	25	0.9068966424308087
1107	1	0.48536662832737676
1107	2	0.5381028281497322
1107	3	0.62352220822779
1107	4	0.6956633606647671
1107	5	0.6254947731952996
1107	6	0.5523098188312119
1107	7	0.6716774664673153
1107	8	0.5852084618353928
1107	9	0.8412777821769524
1107	10	0.780665958355172
1107	11	0.8155188088395064
1107	12	0.9512946018633457
1107	13	0.9898932692531162
1107	14	1.1506776782531958
1107	15	1.1515714531499768
1107	16	1.066996665130766
1107	17	1.1626288939034097
1107	18	1.4534612602708137
1107	19	1.3128127563170011
1107	20	1.1682911462663057
1107	21	1.1151046078186084
1107	22	1.1814602640241745
1107	23	1.1059929415254288
1107	24	1.0315648955765298
1107	25	1.1375924730028402
1108	1	0.5706271564909251
1108	2	0.6961400914887754
1108	3	0.5108957278063748
1108	4	0.5983578469514228
1108	5	0.4823365256015961
1108	6	0.6591743617899013
1108	7	0.6592506855821156
1108	8	0.668242231575654
1108	9	0.7321907674192474
1108	10	0.779284522238882
1108	11	0.6650614403181336
1108	12	0.6727416119000509
1108	13	0.620306239778003
1108	14	0.8434331689187857
1108	15	0.8201580988293315
1108	16	0.8701007240781422
1108	17	0.9763410619436499
1108	18	1.135220273684233
1108	19	0.9198391907858081
1108	20	0.873592728859407
1108	21	0.8327023721440057
1108	22	0.7639522044560962
1108	23	0.9865187217124856
1108	24	1.050456191643093
1108	25	0.935381260736925
1109	1	0.36131660152854295
1109	2	0.29605783901213556
1109	3	0.4961294706684676
1109	4	0.6508075678305758
1109	5	0.5141220234964123
1109	6	0.47719039301029653
1109	7	0.6264038889052514
1109	8	0.571998238621461
1109	9	0.7950248998952674
1109	10	0.8084449627893235
1109	11	0.2725519225003339
1109	12	0.48522738051710235
1109	13	0.695543363936558
1109	14	0.45489379721058043
1109	15	0.8898732643008884
1109	16	0.7459107019183406
1109	17	0.6443429109490211
1109	18	-0.8729883661473821
1109	19	0.3471408069464559
1109	20	0.591751252370635
1109	21	0.7401936847178376
1109	22	0.8627454997283621
1109	23	0.48429203382858077
1109	24	0.7395783963008355
1109	25	0.81595797792491
1110	1	0.3607998264030776
1110	2	0.3058396331146156
1110	3	0.3272991535046401
1110	4	0.20227278671261217
1110	5	0.3974627289453494
1110	6	0.3651602635954987
1110	7	0.44355928176049986
1110	8	0.2781964575066951
1110	9	0.5442418125463107
1110	10	0.6589412210407843
1110	11	0.6953052375531428
1110	12	0.08717632360000493
1110	13	0.3850797291142314
1110	14	0.6164927523767787
1110	15	0.6453208505946293
1110	16	0.7203925672409781
1110	17	0.2867393225463033
1110	18	0.824976493725714
1110	19	0.749825827418249
1110	20	0.7220619019805241
1110	21	0.7173593200153547
1110	22	0.6523325245722447
1110	23	0.7373574234761548
1110	24	0.889863844035439
1110	25	0.885455362496046
1111	1	0.263047471042551
1111	2	0.5564326009361196
1111	3	0.5381298822222753
1111	4	0.4619565332233151
1111	5	0.38277950988239695
1111	6	0.4885383617533776
1111	7	0.4476950399389992
1111	8	0.2615660148958483
1111	9	0.5525058579719655
1111	10	0.5871442259689061
1111	11	0.5049367921374142
1111	12	0.48836937156966087
1111	13	0.45535892977239456
1111	14	0.41041831739584467
1111	15	0.8011737425772125
1111	16	0.6076877404515386
1111	17	0.5068307189321075
1111	18	0.761362708602249
1111	19	0.6182660134982285
1111	20	0.44614920070989683
1111	21	0.698165302019973
1111	22	0.38432750530171944
1111	23	0.8918084698996225
1111	24	0.9269000897071606
1111	25	0.5950291817228148
1112	1	-0.08261307266919005
1112	2	0.2887012095408359
1112	3	0.35273377477835766
1112	4	0.4535180740034761
1112	5	0.5433437325394124
1112	6	0.48349000057572766
1112	7	0.06928955870827735
1112	8	0.30573285843203024
1112	9	0.6173809999877804
1112	10	0.6001105446633943
1112	11	0.4922770968048056
1112	12	0.5312104369245816
1112	13	0.27317025429614006
1112	14	0.42942807405312067
1112	15	0.42941959535875895
1112	16	0.5748011586941288
1112	17	0.5969882427827429
1112	18	0.6845386191532441
1112	19	0.5599289495558892
1112	20	0.36061926695719393
1112	21	0.35093582170718207
1112	22	0.331629308895117
1112	23	0.5951565354227952
1112	24	0.47645848072373553
1112	25	0.6904049506592926
1113	1	-0.35713372451672143
1113	2	-0.11782785335456936
1113	3	-0.32665533945811376
1113	4	-0.14857869991012507
1113	5	-0.2844970789201611
1113	6	-0.2558360741933825
1113	7	-0.42461260514921406
1113	8	-0.17215212578206973
1113	9	-0.28514387647604167
1113	10	-0.47962561642049906
1113	11	-0.4873253310478063
1113	12	-0.0326092132688629
1113	13	-0.3182656118977363
1113	14	-0.30498345633755974
1113	15	-0.708515688518446
1113	16	-0.5413927119673545
1113	17	-0.5263513736693142
1113	18	-0.6735253818742706
1113	19	-0.664366672915456
1113	20	-0.6349461009089978
1113	21	-0.45351555962431433
1113	22	-0.25463079486312523
1113	23	-0.7362679968580323
1113	24	-0.2101722749688264
1113	25	-0.29566457673562496
1114	1	-1.4709096359057625
1114	2	-1.1219212053537397
1114	3	-0.9349084940708821
1114	4	-1.2294176857134715
1114	5	-1.0732127877196476
1114	6	-1.42194710901693
1114	7	-1.6587104861438249
1114	8	-1.1209756471937489
1114	9	-1.2004746211016766
1114	10	-1.3435018837385684
1114	11	-2.07057190859987
1114	12	-1.5058926004251418
1114	13	-1.3930162648876205
1114	14	-1.6764533588449737
1114	15	-1.5700663305697233
1114	16	-2.3213364717004197
1114	17	-4.053266724156089
1114	18	-3.2178692485951608
1114	19	-2.148025656668544
1114	20	-1.3770833509678937
1114	21	-2.4370079050078175
1114	22	-2.342831847748542
1114	23	-2.246582685266219
1114	24	-1.8099001842707594
1114	25	-1.8822287129120028
1115	1	-1.24944729513399
1115	2	-0.8524517856290963
1115	3	-0.8924000644502428
1115	4	-0.780789195746685
1115	5	-0.682292598017952
1115	6	-0.8175276588076912
1115	7	-2.0321409745945402
1115	8	-1.3179626419171697
1115	9	-1.1308451387167295
1115	10	-0.88232368014895
1115	11	-1.6962099711741345
1115	12	-1.3430998259414153
1115	13	-1.0396754945990523
1115	14	-1.136244995533886
1115	15	-2.2084098674649795
1115	16	-1.0384909165915417
1115	17	-1.1003382005153242
1115	18	-1.276246281457702
1115	19	-1.31617630248207
1115	20	-1.2180404040348236
1115	21	-1.9518639153681292
1115	22	-2.0911823419549904
1115	23	-1.5056125779693446
1115	24	-1.3446033711346388
1115	25	-1.658830044146768
1116	1	-1.0304880090229567
1116	2	-1.6044259580336906
1116	3	-1.2538700622488612
1116	4	-0.9563372449001726
1116	5	-1.3792816625475766
1116	6	-1.0503745986691655
1116	7	-0.8463155368331279
1116	8	-1.4643817528215877
1116	9	-1.5628363098660998
1116	10	-2.551158537197672
1116	11	-1.1116944281108943
1116	12	-1.144640501196875
1116	13	-1.5730988755998183
1116	14	-1.4339799055032287
1116	15	-1.003442035329769
1116	16	-0.9534649738245378
1116	17	-1.3192076819369802
1116	18	-1.0305520226889868
1116	19	-1.3937486018174283
1116	20	-1.732145717527537
1116	21	-2.2105272250499794
1116	22	-1.4497831090853026
1116	23	-1.4362126511543352
1116	24	-2.016170565015095
1116	25	-1.6719894926643377
1201	1	0.6442486518668993
1201	2	0.6780242328250772
1201	3	0.9199300656290177
1201	4	0.867467791153544
1201	5	0.9884446198349579
1201	6	0.8993345808577615
1201	7	0.8942189797292386
1201	8	0.33162605781067356
1201	9	0.7236660002191826
1201	10	0.9433578659849502
1201	11	0.5480460605665485
1201	12	0.0942704851283338
1201	13	0.40748214040142017
1201	14	0.9790635791660165
1201	15	0.6449480387763131
1201	16	1.0299465709714823
1201	17	1.017522364559511
1201	18	1.3114988991194991
1201	19	0.8609932413986314
1201	20	0.9710897578830924
1201	21	0.7093367079346962
1201	22	1.0115680277101262
1201	23	1.3088761514541574
1201	24	1.144828951831741
1201	25	1.036019441689625
1202	1	0.6377403807586255
1202	2	0.6961811463631697
1202	3	0.32026450985903665
1202	4	0.8021033353939586
1202	5	0.7141635481289299
1202	6	0.8008581670004986
1202	7	0.7962364695480844
1202	8	0.9357522101494951
1202	9	1.2211083399803826
1202	10	1.2996022063498387
1202	11	0.822396408680504
1202	12	0.9044389010242995
1202	13	0.9182583330690122
1202	14	1.0381285208501296
1202	15	0.7208372509551069
1202	16	0.927158937799959
1202	17	1.1754036074772358
1202	18	1.4108220933556979
1202	19	1.1963389925280836
1202	20	1.0582397883152268
1202	21	1.0931168740991097
1202	22	1.202182682639389
1202	23	1.361879407445329
1202	24	1.4707543485628847
1202	25	1.320420745075758
1203	1	0.4349706424718143
1203	2	0.4978238240934907
1203	3	0.6496585440221452
1203	4	0.7429071343927021
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2211	16	-0.2845652706250661
2211	17	-0.05485977985034651
2211	18	0.016185141565713934
2211	19	-0.14823617853387816
2211	20	-0.4453908441029739
2211	21	-0.26894585062640886
2211	22	-0.33729835924523854
2211	23	0.1768189194527423
2211	24	-0.05473944630706029
2211	25	-0.028467201819871946
2212	1	0.05864415481748253
2212	2	-0.0933749645030666
2212	3	-0.006879933321542353
2212	4	-0.17061880467704071
2212	5	-0.0018261411024695917
2212	6	-0.0970270708983918
2212	7	-0.1487276406356699
2212	8	-0.13060899614644939
2212	9	0.006117202833650026
2212	10	0.11366446196969093
2212	11	-0.2722071445179495
2212	12	0.042964231248525664
2212	13	-0.09771020617162662
2212	14	-0.037655391412364736
2212	15	-0.18041717454715922
2212	16	0.040670997441750956
2212	17	0.10357865356881746
2212	18	-2.6425404589564643
2212	19	-0.9239739443939925
2212	20	-0.32290348421039994
2212	21	-0.35627836650128586
2212	22	-0.06954493391661032
2212	23	-0.1673650461621321
2212	24	-0.5018795184374412
2212	25	-0.2409408877746202
2213	1	-0.19670038629127617
2213	2	-0.12795202630495695
2213	3	-0.5323665635627961
2213	4	-0.5359820568387121
2213	5	-0.3731414014193035
2213	6	-0.4440502875543757
2213	7	-0.256577249140868
2213	8	-0.6119035712687477
2213	9	-0.503059669577631
2213	10	-0.48718473641047666
2213	11	-0.5459097597072358
2213	12	-0.5455757322760348
2213	13	-0.4056910200548103
2213	14	-0.5628698556363858
2213	15	-0.6987346979114477
2213	16	-1.1820277638421004
2213	17	-0.8601410102383298
2213	18	-0.7178392426947298
2213	19	-0.5918501576324573
2213	20	-1.31443920765971
2213	21	-0.8321732024758077
2213	22	-0.8781803316036871
2213	23	-1.342602296713899
2213	24	-1.0329495926726722
2213	25	-0.9787895986803434
2214	1	-1.9095848239166209
2214	2	-3.87301345305179
2214	3	-2.1299694998476575
2214	4	-1.7547403465025297
2214	5	-1.8264856091951067
2214	6	-1.6960644103219664
2214	7	-1.8480454672746873
2214	8	-1.6384236976648268
2214	9	-1.7251195339087175
2214	10	-1.7581332431238579
2214	11	-1.5579803599495747
2214	12	-2.051819138203328
2214	13	-1.9505497174484177
2214	14	-3.057333422365968
2214	15	-3.291097910057779
2214	16	-2.513018426272076
2214	17	-3.0673793225944834
2214	18	-2.3909470441287297
2214	19	-4.609062068205979
2214	20	-2.9899246990389265
2214	21	-2.7235424351679143
2214	22	-2.9328030845978574
2214	23	-2.8393565722685423
2214	24	-2.9722979511319103
2214	25	-3.0482011505455766
2215	1	-0.9480789039721245
2215	2	-2.5664211944503457
2215	3	-1.3085030276349165
2215	4	-0.9820370948096091
2215	5	-0.8785381563108032
2215	6	-1.2827821887733855
2215	7	-1.915470854332682
2215	8	-1.6942671415679733
2215	9	-1.4490072121017903
2215	10	-3.025872748061982
2215	11	-1.967660250915344
2215	12	-2.328703706247481
2215	13	-1.4066998002345485
2215	14	-2.755024878725621
2215	15	-2.5864987246319586
2215	16	-2.173910988886803
2215	17	-2.445307847250909
2215	18	-1.3841120363752353
2215	19	-1.9744775941266681
2215	20	-2.105629717843833
2215	21	-1.8581893668297695
2215	22	-1.513178673469426
2215	23	-2.020883608445409
2215	24	-1.8721492463907836
2215	25	-1.7392504618015465
2216	1	-2.173571808752669
2216	2	-2.176965404306138
2216	3	-2.6013494807926527
2216	4	-3.389349507030733
2216	5	-2.499413220586046
2216	6	-2.5525471140606086
2216	7	-2.501790780042011
2216	8	-3.0778848209054956
2216	9	-3.3043859947478573
2216	10	-2.954308115946617
2216	11	-3.3771438233142135
2216	12	-3.8668352345017283
2216	13	-4.210492615221618
2216	14	-3.764260559736222
2216	15	-3.5928456651303513
2216	16	-2.8719539516109975
2216	17	-2.976213686896646
2216	18	-3.0848065927417494
2216	19	-3.4297949795248712
2216	20	-3.143721337886962
2216	21	-3.4440818142008176
2216	22	-3.958497517052262
2216	23	-3.972538648008257
2216	24	-3.806860180473162
2216	25	-4.022643273718753
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80876.0
3	1.0
4	615.0
5	4066.0
6	5172.0
7	4483.0
8	3984.0
9	3652.0
10	3390.0
11	3343.0
12	3218.0
13	2995.0
14	3110.0
15	2999.0
16	3117.0
17	3327.0
18	3899.0
19	4566.0
20	5809.0
21	7416.0
22	9463.0
23	11471.0
24	13247.0
25	14878.0
26	16835.0
27	18360.0
28	21271.0
29	25124.0
30	30862.0
31	38365.0
32	48240.0
33	62417.0
34	80484.0
35	106689.0
36	145021.0
37	225241.0
38	643334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38308535847976	18.487360440525602	12.758377192797003	25.371177008197638
2	15.05385149920695	19.830635439260348	40.74835699635932	24.367156065173383
3	18.765572895905315	24.176299934125673	29.618157710608173	27.439969459360835
4	12.512193300849978	16.614682058927663	37.50749662200406	33.365628018218295
5	13.329619939120407	37.59667119299313	35.80924394290205	13.264464924984418
6	32.643582250574035	36.516290455222475	17.742803578975504	13.097323715227985
7	28.18568708230009	31.5323434661966	21.910427920971976	18.37154153053134
8	25.53134612501603	36.12750259945825	20.41515470614833	17.92599656937739
9	26.339228465208684	15.19800117400924	19.984707777058656	38.478062583723414
10	14.85921288778994	28.890422117505114	34.558571641010175	21.69179335369477
11	34.13313457708507	22.64978362380162	24.182538669569343	19.03454312954396
12	23.038803989671212	24.88469219242271	31.346745824325144	20.729757993580932
13	28.646899776218206	21.85800903435475	25.6039007991003	23.89119039032675
14	21.073689242816794	22.09593504060865	27.528203880776925	29.30217183579763
15	23.61160617518846	30.0814648009441	23.35372462127598	22.95320440259146
16	22.460050163821123	28.201375350851738	27.1616741663546	22.176900318972546
17	22.120445484287757	28.438517123497988	27.495312878175778	21.945724514038485
18	21.9540399581798	27.583798588116192	28.88962539583063	21.572536057873375
19	23.180360598372214	27.49553556914118	27.669657403850668	21.654446428635936
20	23.46444831642919	27.362375627207342	27.724099813719267	21.4490762426442
21	22.983738168191255	27.031524797351768	27.407589818187073	22.5771472162699
22	23.14242143103553	27.60005611101311	26.88554376809535	22.371978689856007
23	22.398942767348608	27.537177742724	27.353004924922033	22.71087456500536
24	22.90819047986004	27.258513713813027	27.69541235356942	22.137883452757514
25	22.847363541127315	27.048598986116296	27.351921201247485	22.752116271508903
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	243.0
1	243.0
2	404.5
3	566.0
4	566.0
5	566.0
6	1134.0
7	1702.0
8	1702.0
9	1702.0
10	2354.5
11	3007.0
12	3007.0
13	3007.0
14	5285.5
15	7564.0
16	7564.0
17	7564.0
18	13276.0
19	18988.0
20	18988.0
21	18988.0
22	29453.0
23	39918.0
24	39918.0
25	39918.0
26	55881.5
27	71845.0
28	71845.0
29	71845.0
30	92504.0
31	113163.0
32	113163.0
33	113163.0
34	131119.0
35	149075.0
36	149075.0
37	149075.0
38	163913.5
39	178752.0
40	178752.0
41	178752.0
42	190512.0
43	202272.0
44	202272.0
45	202272.0
46	214642.5
47	227013.0
48	227013.0
49	227013.0
50	219440.5
51	211868.0
52	211868.0
53	211868.0
54	189731.5
55	167595.0
56	167595.0
57	167595.0
58	143714.0
59	119833.0
60	119833.0
61	119833.0
62	97871.0
63	75909.0
64	75909.0
65	75909.0
66	58693.5
67	41478.0
68	41478.0
69	41478.0
70	30287.5
71	19097.0
72	19097.0
73	19097.0
74	13268.0
75	7439.0
76	7439.0
77	7439.0
78	5128.5
79	2818.0
80	2818.0
81	2818.0
82	1841.0
83	864.0
84	864.0
85	864.0
86	563.5
87	263.0
88	263.0
89	263.0
90	158.0
91	53.0
92	53.0
93	53.0
94	30.5
95	8.0
96	8.0
97	8.0
98	7.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3529078936280352
2	0.03984735213743123
3	0.036235809647633836
4	0.03563388589933427
5	0.04123177675852023
6	0.0184188666979667
7	0.018599443822456572
8	0.025100220304091876
9	0.021970216812934137
10	0.021488677814294484
11	0.02070617694150505
12	0.021127523565314745
13	0.02088675406599492
14	0.029313686542188834
15	0.03009618741497827
16	0.0229934871850434
17	0.02443810418096236
18	0.11165685530956938
19	0.02612349067620114
20	0.02443810418096236
21	0.027507915297290138
22	0.02088675406599492
23	0.024076949931982616
24	0.018900405696606355
25	0.024076949931982616
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1661340.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.26402726568983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.62628485310373	60.535343863404464
2	11.980642346743737	17.55500215104623
3	3.076227200329447	6.7613038044118
4	1.088026903638103	3.1885293093578433
5	0.43935535944189164	1.6094471516738855
6	0.2328407542537356	1.0235310820925685
7	0.14013156606787494	0.7186622014026425
8	0.0933254320166046	0.5469917596677443
9	0.057143005963822865	0.37678740722792964
>10	0.23811103901208835	2.9560214933092803
>50	0.014881059289392679	0.7432405052097978
>100	0.010721509898241737	1.8066031222523364
>500	0.00123694834360711	0.66064762718593
>1k	0.0010720218977928288	1.5178885217575953
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3946	0.2375191110790085	No Hit
CCCATGTACTCTGCGTTGATACCAC	3379	0.20339003455042315	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2992	0.18009558549122998	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1859	0.1118976248088892	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1800	0.10834627469392179	No Hit
GTCCTACAGTGGACATTTCTAAATT	1755	0.10563761782657374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.2038474965991308E-4	0.0	0.0	0.0
6	0.0	1.2038474965991308E-4	0.0	0.0	0.0
7	0.0	1.2038474965991308E-4	0.0	0.0	0.0
8	0.0	1.2038474965991308E-4	0.0	0.0	0.0
9	0.0	1.2038474965991308E-4	0.0	6.019237482995654E-5	0.0
10	0.0	1.2038474965991308E-4	0.0	6.019237482995654E-5	0.0
11	0.0	1.2038474965991308E-4	0.0	1.2038474965991308E-4	0.0
12	0.0	1.2038474965991308E-4	0.0	1.2038474965991308E-4	0.0
13	0.0	1.2038474965991308E-4	0.0	1.2038474965991308E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACCG	40	0.005286916	14.246905	5
TACACCG	65	5.464048E-5	13.150989	5
GGACCGT	70	1.09689594E-4	12.207219	6
AAAGCCG	55	0.0030735938	12.088282	5
TGGACCG	65	8.0385606E-4	11.689768	5
GTCTTAG	140	6.4574124E-10	11.571901	1
CCTAGAC	90	9.5389434E-5	10.552945	3
CCCAATA	100	2.4051988E-5	10.447415	2
GGCGAGG	350	0.0	10.037047	19
GGACGTG	975	0.0	10.030092	6
AGGACGT	1000	0.0	9.877854	5
CTCGAAC	155	4.0505256E-8	9.808163	18
TAGGACA	385	0.0	9.621286	4
GACGTGG	575	0.0	9.5770645	7
GTATAAG	170	1.5808837E-8	9.529801	1
ATAAGCC	120	1.7050419E-5	9.497651	3
CTTAGAC	190	1.0550139E-9	9.497651	3
CTTACAC	190	1.0550139E-9	9.497651	3
GTTGGAC	110	6.853956E-5	9.49765	3
ATACTGC	150	2.6694215E-7	9.494505	6
>>END_MODULE