FastQCFastQC Report
Thu 26 May 2016
SRR1512959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512959_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661340
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC38770.23336583721574153No Hit
GTACATGGGAAGCAGTGGTATCAAC38240.23017564134975382No Hit
GTATCAACGCAGAGTACTTTTTTTT37090.22325351824430884No Hit
TATCAACGCAGAGTACTTTTTTTTT28180.16962211227081753No Hit
GGTATCAACGCAGAGTACTTTTTTT23290.14018804097896878No Hit
GTCCTACAGTGGACATTTCTAAATT17320.10425319320548473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG508.6123764E-515.2210235
TCCAACG951.0521362E-611.98838518
CTGAGCG855.3184922E-511.1774279
TAGGACC3550.010.7190294
GCGCCAC1201.5371515E-610.28449213
CTAAGAC1855.820766E-1110.2773433
GGCGAGG4000.010.2032319
GTCCTAC9600.010.2015731
GCGTGCG750.002645853710.1342019
CGTGCGC750.002647838310.13328510
CTAGACA1606.031769E-910.107714
TGTAGGA11050.09.9788152
GTATTAT1151.015725E-59.9216641
TTAATAC1752.5411282E-99.7781573
ATACTGT2550.09.6976236
AGGACGT9500.09.61327655
TCCTACA10800.09.5936982
GTAGGAC10050.09.5538383
AAGGCGT1201.6766566E-59.5111296
GTCTTAG2106.548362E-119.5082621
Produced by FastQC (version 0.11.5)