##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512959_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1661340
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.29140814041677	31.0	31.0	34.0	26.0	34.0
2	30.435096367992102	31.0	31.0	34.0	26.0	34.0
3	30.536466346443234	31.0	31.0	34.0	27.0	34.0
4	33.98417482273346	37.0	35.0	37.0	30.0	37.0
5	33.612698785317875	37.0	35.0	37.0	28.0	37.0
6	33.461357097282914	37.0	35.0	37.0	28.0	37.0
7	33.313375949534716	37.0	35.0	37.0	27.0	37.0
8	33.20195143679199	37.0	35.0	37.0	26.0	37.0
9	34.63709957022645	39.0	35.0	39.0	27.0	39.0
10	34.53655964462422	39.0	35.0	39.0	27.0	39.0
11	34.55861292691442	39.0	35.0	39.0	27.0	39.0
12	34.411539480178654	39.0	35.0	39.0	26.0	39.0
13	34.38441559223278	39.0	35.0	39.0	26.0	39.0
14	35.4281236832918	40.0	36.0	41.0	26.0	41.0
15	35.43374143763468	40.0	36.0	41.0	26.0	41.0
16	35.40269722031613	40.0	36.0	41.0	26.0	41.0
17	35.37992524107046	40.0	36.0	41.0	25.0	41.0
18	35.304765430315285	40.0	36.0	41.0	25.0	41.0
19	35.28666738897517	40.0	36.0	41.0	25.0	41.0
20	35.26372927877497	40.0	36.0	41.0	25.0	41.0
21	35.20537036368233	40.0	36.0	41.0	25.0	41.0
22	35.17005971083583	40.0	36.0	41.0	25.0	41.0
23	35.055075421045665	40.0	35.0	41.0	24.0	41.0
24	35.03441318453778	40.0	35.0	41.0	24.0	41.0
25	34.93860136997845	40.0	35.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.600975900019435
1101	2	0.6316028827159528
1101	3	0.613875720019049
1101	4	0.5107104598429615
1101	5	0.6437565627324773
1101	6	0.7314666956482512
1101	7	0.7035521639527147
1101	8	0.6703102265798009
1101	9	0.6284845009539879
1101	10	0.9415187094254378
1101	11	1.0827079356144083
1101	12	1.0824491283043045
1101	13	1.2100553923138477
1101	14	1.1795181864597097
1101	15	1.0634198323750113
1101	16	1.27726483200761
1101	17	1.2357544299568985
1101	18	0.7012285662739259
1101	19	0.9995806344238716
1101	20	0.8935900574535012
1101	21	1.1332061503617936
1101	22	0.9142715644937667
1101	23	1.0382250594237306
1101	24	1.0439655318090217
1101	25	1.0609531330983444
1102	1	0.6307941840863407
1102	2	0.6066161625806856
1102	3	0.5904473577194018
1102	4	0.48997204948641127
1102	5	0.6185572400679291
1102	6	0.6704404177262475
1102	7	0.7206277008208559
1102	8	0.7219171525592074
1102	9	0.9024499807872957
1102	10	0.9137083034980051
1102	11	0.9130536111168652
1102	12	0.9254181569164857
1102	13	0.9914544790581701
1102	14	1.1247636831515848
1102	15	1.0235518703915858
1102	16	0.9742108371998555
1102	17	1.0802590598289825
1102	18	1.0102310676268544
1102	19	1.1091005517271029
1102	20	1.0046075444791356
1102	21	0.9816732300135484
1102	22	1.0987101815922529
1102	23	1.1067363803525438
1102	24	0.9862707542249396
1102	25	1.103907572563358
1103	1	0.8323589851520481
1103	2	0.6820074010390762
1103	3	0.7453495251896278
1103	4	0.7666954235782057
1103	5	0.8457700823442877
1103	6	0.789015765884173
1103	7	0.8393930921815311
1103	8	0.9476237038555979
1103	9	1.0151486553734443
1103	10	1.150828446244283
1103	11	1.022608934465289
1103	12	1.1369695449651545
1103	13	1.1255745668080266
1103	14	1.2370188726753781
1103	15	1.060630307515389
1103	16	1.22148270897263
1103	17	1.2713753039397844
1103	18	1.3572795928060515
1103	19	1.3566570300004557
1103	20	1.3518248781302304
1103	21	0.9811356399569036
1103	22	1.2343090621094603
1103	23	1.1987553296763949
1103	24	1.3780776617759471
1103	25	1.2987042996649905
1104	1	0.5144355454001825
1104	2	0.5619137007077377
1104	3	0.47122630229746676
1104	4	0.46886689397474157
1104	5	0.5489691411066175
1104	6	0.5382804239520667
1104	7	0.6637101126440754
1104	8	0.620627241058223
1104	9	0.7799302758326618
1104	10	0.7482414036874587
1104	11	0.7575139713873966
1104	12	0.6423532308845452
1104	13	0.7327407669389885
1104	14	0.945504227083724
1104	15	0.9918266502384157
1104	16	1.0011715440246647
1104	17	0.9888806006701003
1104	18	1.0146371708732786
1104	19	0.9368603397574802
1104	20	1.023313875600202
1104	21	0.7459254266812749
1104	22	1.0221478297898017
1104	23	0.8841053524360802
1104	24	0.9571665787930783
1104	25	1.0357567618695427
1105	1	0.3580231048999174
1105	2	0.4773660328172227
1105	3	0.46152886242159497
1105	4	0.3403981312801463
1105	5	0.37516494012946566
1105	6	0.4559670044835116
1105	7	0.5276046998706647
1105	8	0.5919429366496658
1105	9	0.6077213279307543
1105	10	0.4797082288313277
1105	11	0.5244629206066378
1105	12	0.5515623936724836
1105	13	0.6255133838425024
1105	14	0.8367428379006867
1105	15	0.6585415495295948
1105	16	0.7515349279222789
1105	17	0.4065355890808604
1105	18	0.6794633978173579
1105	19	0.7469474356577521
1105	20	0.7371125092111726
1105	21	0.684580383201876
1105	22	0.7485333901639848
1105	23	0.7418750917742614
1105	24	0.857165893303673
1105	25	0.8240923846381634
1106	1	0.4827942686174964
1106	2	0.5238693881586229
1106	3	0.45089460492064504
1106	4	0.38679730930066114
1106	5	0.483755644214952
1106	6	0.5505948835310122
1106	7	0.6601319672630623
1106	8	0.8031325438967869
1106	9	0.7440124022834453
1106	10	0.7989888100182156
1106	11	0.6003585792867128
1106	12	0.6647904476036572
1106	13	0.6651948755450121
1106	14	0.8957385714454489
1106	15	0.8269233241012515
1106	16	0.9614567526186946
1106	17	0.6541077395356751
1106	18	0.8758577828056744
1106	19	0.8932768849084383
1106	20	1.0133021988955306
1106	21	0.9498378740838049
1106	22	0.8697435571117893
1106	23	0.9531916377737275
1106	24	0.9136937624149368
1106	25	0.9036955303263525
1107	1	0.5432342420175651
1107	2	0.5241980293530233
1107	3	0.5992263793516166
1107	4	0.6097647849676378
1107	5	0.6648007917991379
1107	6	0.5612910113089953
1107	7	0.6006161900566411
1107	8	0.6450332673932309
1107	9	0.5963969227687969
1107	10	0.7702978621970118
1107	11	0.6982882254944656
1107	12	0.5862014785739404
1107	13	0.6797079564647603
1107	14	0.7776566471969559
1107	15	0.7364724083782832
1107	16	0.777178633006443
1107	17	0.7160058796265574
1107	18	0.8427547555058794
1107	19	0.9718119853597642
1107	20	0.8922593603729538
1107	21	0.8754289352719411
1107	22	0.9211324662430727
1107	23	0.9152110035990972
1107	24	1.0595873049583915
1107	25	0.9060804651853829
1108	1	0.5264706833535371
1108	2	0.5326046711058297
1108	3	0.47899942831601194
1108	4	0.5223840924377612
1108	5	0.5931365785104319
1108	6	0.6626343822017304
1108	7	0.544674314395607
1108	8	0.6850163266541003
1108	9	0.6346314679879654
1108	10	0.8491217382369811
1108	11	0.746199825014024
1108	12	0.7677148684164123
1108	13	0.7973635044853822
1108	14	0.8782680055510355
1108	15	0.7995780905273833
1108	16	0.7366181654557593
1108	17	0.7965308310662493
1108	18	0.8606659863757429
1108	19	0.7987079521321405
1108	20	0.9248099846912012
1108	21	0.9255309997959245
1108	22	0.951779257833401
1108	23	1.048520160371524
1108	24	0.9174917115224162
1108	25	1.0005432325671464
1109	1	0.30754477599406727
1109	2	0.20476372286268685
1109	3	0.3008077270169167
1109	4	0.4058715508289481
1109	5	0.46261766095069845
1109	6	0.22362613786962982
1109	7	0.33453191097125057
1109	8	0.635597826606535
1109	9	0.7738307461008418
1109	10	0.5291732982733492
1109	11	0.6716478467953451
1109	12	0.8834191621675984
1109	13	0.7052720524348928
1109	14	0.7979925894119191
1109	15	0.8643898662383052
1109	16	0.6918609304501189
1109	17	0.763234603127529
1109	18	0.7819365555043163
1109	19	1.0299948945441884
1109	20	0.8341695327600291
1109	21	1.0169380343202548
1109	22	1.0068454407750878
1109	23	0.88642931581807
1109	24	0.41278987383659427
1109	25	0.5621102893867658
1110	1	0.33879498501608296
1110	2	0.3052093069072761
1110	3	0.35842044695533204
1110	4	0.4402959863531848
1110	5	0.34252880587369106
1110	6	0.34092318585351933
1110	7	0.3846055885053161
1110	8	0.5684678072063818
1110	9	0.5097923913243889
1110	10	0.670614242974338
1110	11	0.5932544852473072
1110	12	0.6718496227605186
1110	13	0.21853648047940766
1110	14	0.6290318425104644
1110	15	0.7017210091663273
1110	16	0.7128736410243519
1110	17	0.6464902457041006
1110	18	0.5510671954999182
1110	19	0.648450978691578
1110	20	0.6534286685852209
1110	21	0.6682776800457546
1110	22	0.596399487397683
1110	23	0.7234757525852373
1110	24	0.7265660168562178
1110	25	0.6909843215966589
1111	1	-0.3097535773824802
1111	2	-0.10181226286688982
1111	3	0.00823757349767007
1111	4	0.0138818212282672
1111	5	0.353555751132383
1111	6	0.23494159960744554
1111	7	0.4969080309320759
1111	8	0.49523938168723447
1111	9	0.20753225528769548
1111	10	0.14495667400942125
1111	11	0.3399011577094271
1111	12	0.2620531996631499
1111	13	0.36739000629580687
1111	14	0.5544650743487054
1111	15	0.5611161386569918
1111	16	0.3058931613726301
1111	17	0.5606205560794635
1111	18	0.5070415947100315
1111	19	0.5248630967813028
1111	20	0.4980678121415565
1111	21	0.3840165118251804
1111	22	0.30946282924090696
1111	23	0.4229660557645971
1111	24	0.6169438457617318
1111	25	0.6779713039470323
1112	1	0.09527299466048689
1112	2	-0.19194362786551622
1112	3	0.04674756528735813
1112	4	-0.10306236707741334
1112	5	0.16885582586346715
1112	6	0.1281088480113084
1112	7	0.1501822571502629
1112	8	0.26837644039780173
1112	9	0.11576388122087167
1112	10	0.36977387416803964
1112	11	0.15962921266203978
1112	12	0.23890960123957683
1112	13	0.2699903448151346
1112	14	0.2833799992222623
1112	15	0.23948814844753485
1112	16	-0.04597030012124037
1112	17	0.22523865764834028
1112	18	0.23632883733517218
1112	19	0.09228411934272174
1112	20	0.3405585272353946
1112	21	0.38352112856529885
1112	22	0.3285254981215644
1112	23	0.5584088627045389
1112	24	0.38517062041689343
1112	25	0.47243525853526336
1113	1	-0.3345726003005218
1113	2	-0.6053628594157949
1113	3	-0.36854401828419725
1113	4	-0.24008165208627474
1113	5	-0.23895502796376178
1113	6	-0.4441912767832292
1113	7	-0.18637334411121742
1113	8	-0.18833773150623756
1113	9	-0.12720492708672992
1113	10	-0.5260626050638209
1113	11	-0.2405117235920784
1113	12	-0.2090242593897429
1113	13	-0.1593232678566352
1113	14	-0.11263265032724235
1113	15	-0.09188171028642955
1113	16	-0.44929748918931267
1113	17	-0.15544587251496722
1113	18	-0.05695872642893818
1113	19	-0.286077218996283
1113	20	0.008699643999001694
1113	21	-0.2577071517173337
1113	22	-0.4315895419636959
1113	23	-0.24168729331119465
1113	24	-0.557674261184502
1113	25	-0.631215372293326
1114	1	-0.7559627957053969
1114	2	-0.5132443790231882
1114	3	-0.4755660670484758
1114	4	-0.36587482952096906
1114	5	-0.5345010088203139
1114	6	-0.9121470619716732
1114	7	-0.7460147447743495
1114	8	-1.0317545507358545
1114	9	-0.8264242054862905
1114	10	-0.806308858405913
1114	11	-0.8535182029656951
1114	12	-0.8088062768064148
1114	13	-0.6311948719326423
1114	14	-1.762993909736025
1114	15	-1.1156739257665862
1114	16	-0.9655259020623035
1114	17	-1.119742852360865
1114	18	-1.1997826523353368
1114	19	-1.182323007886012
1114	20	-0.5495293886312638
1114	21	-0.6816864935316076
1114	22	-0.8450245283584294
1114	23	-0.8207385908749742
1114	24	-1.3259989613487733
1114	25	-1.1949455915730383
1115	1	-1.5923950746624236
1115	2	-1.2606447329708317
1115	3	-0.9614283360219851
1115	4	-2.063565269226004
1115	5	-1.5423102568521685
1115	6	-1.2406753013862541
1115	7	-1.0924103680470765
1115	8	-1.2044261137277061
1115	9	-1.691554873877699
1115	10	-1.4034171981303771
1115	11	-1.2516524864887373
1115	12	-1.5854969108414636
1115	13	-1.0773774739076885
1115	14	-2.0288483461715643
1115	15	-2.087794689261031
1115	16	-1.4598645198863736
1115	17	-1.4921193600873366
1115	18	-1.309726118619075
1115	19	-1.280373180632047
1115	20	-1.2404081904903066
1115	21	-1.307935286386197
1115	22	-1.4345253672410436
1115	23	-1.7810961581005458
1115	24	-1.3067335881387265
1115	25	-1.1453062888469319
1116	1	-1.0019808505326644
1116	2	-0.9975776404122279
1116	3	-0.9288795302348305
1116	4	-0.9813059231965795
1116	5	-1.1101020091078553
1116	6	-1.7494502108890764
1116	7	-1.0745393806474368
1116	8	-1.2314602409675395
1116	9	-1.422897002044735
1116	10	-1.4905934966901526
1116	11	-1.4053860143988999
1116	12	-1.8746328760159017
1116	13	-1.573490359568531
1116	14	-1.8278736154033979
1116	15	-1.66211477591672
1116	16	-1.8417235352712567
1116	17	-1.8729374574816902
1116	18	-2.580771104062265
1116	19	-1.9688907180591713
1116	20	-1.7059536592168456
1116	21	-1.8041680088240994
1116	22	-3.0499222062909226
1116	23	-2.947494625589357
1116	24	-2.2397080914794287
1116	25	-2.678451945930057
1201	1	0.9471672051074798
1201	2	0.9392196466193674
1201	3	0.8305296492036547
1201	4	0.9241863403096744
1201	5	0.8555599210463001
1201	6	1.0368551825918644
1201	7	1.025223346635748
1201	8	1.0701779695793476
1201	9	1.285742330749926
1201	10	1.2695113761409331
1201	11	1.2819002478085295
1201	12	1.2080153516314809
1201	13	1.287611948924912
1201	14	1.3239906512782795
1201	15	1.3943361737194948
1201	16	1.4154586883475915
1201	17	1.307029874407391
1201	18	1.3831417173016547
1201	19	1.0067636980167691
1201	20	1.4336420839649904
1201	21	1.295925762391235
1201	22	1.3919646969566983
1201	23	1.3952951756634207
1201	24	1.442215583290988
1201	25	1.2342248257813964
1202	1	0.69226280655041
1202	2	0.7647853627147541
1202	3	0.7358999316039849
1202	4	0.7237842278740629
1202	5	0.9392996347367983
1202	6	0.8729329750496433
1202	7	0.6444585598338932
1202	8	0.5526652647809698
1202	9	0.8766100919838422
1202	10	0.6855483697427616
1202	11	0.8362794445980626
1202	12	0.8219156628804853
1202	13	0.7906155760396985
1202	14	0.7978564356604068
1202	15	1.0131851090895552
1202	16	0.8322775156959423
1202	17	0.9389701952561254
1202	18	0.8032149894738154
1202	19	1.006540714578783
1202	20	1.0168263493134262
1202	21	1.0848430216186031
1202	22	1.0216513696603116
1202	23	1.0899176563526254
1202	24	0.9418562119249003
1202	25	1.0473875907018027
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2211	5	0.006892561369554073
2211	6	0.12015900532971813
2211	7	0.08820803372063324
2211	8	0.14277463621091613
2211	9	-0.09471012158807923
2211	10	-0.3830580013384477
2211	11	-0.0636314167846237
2211	12	-0.1648996382968022
2211	13	-0.13020648343815822
2211	14	-1.1548692819580708
2211	15	-0.5410991063102273
2211	16	-0.19137533373345406
2211	17	-0.05321852607821853
2211	18	-0.24656238946000286
2211	19	-0.0015366270761631995
2211	20	-0.3538691423469231
2211	21	-0.3504600797438826
2211	22	-0.06742892424158953
2211	23	-0.19290173948897404
2211	24	-0.3892833794504469
2211	25	-0.36748006926669063
2212	1	0.0071897235571327656
2212	2	0.03771029387360514
2212	3	0.10772328593327529
2212	4	0.17208138702152098
2212	5	0.13246066377568155
2212	6	0.05380568172711975
2212	7	0.10650647137597957
2212	8	-0.18638946689259228
2212	9	-0.23031466381374344
2212	10	-0.09624059955954323
2212	11	0.09880977735792129
2212	12	-0.0034790184596502627
2212	13	-0.011216712235267323
2212	14	0.11442588346199756
2212	15	0.09994835478965314
2212	16	0.15007715833811375
2212	17	-0.06420378444936148
2212	18	0.15492719082647
2212	19	0.3180797822255741
2212	20	0.011171540860068774
2212	21	0.20561243103028914
2212	22	0.12507778665044356
2212	23	-0.3195891163839093
2212	24	-0.04693192322650219
2212	25	-0.2384880070668416
2213	1	-0.5298336576106522
2213	2	-0.5342572701712704
2213	3	-0.4963416852612852
2213	4	-0.5223447222137381
2213	5	-0.3954292818690419
2213	6	-0.4810016965983692
2213	7	-1.0234031596933164
2213	8	-0.6927692057344359
2213	9	-0.9249885509130493
2213	10	-0.7125894072963277
2213	11	-0.675298777919636
2213	12	-0.8604248943652237
2213	13	-0.7782163373215312
2213	14	-0.7128717908317697
2213	15	-0.8633683994098007
2213	16	-1.155067991579763
2213	17	-0.9167778313560078
2213	18	-1.0178575350896537
2213	19	-1.2933400210125399
2213	20	-0.9062979385928429
2213	21	-1.7359815498003357
2213	22	-1.1113520821124254
2213	23	-1.298452091617385
2213	24	-1.2500637372981487
2213	25	-1.5688507959979106
2214	1	-1.1585129551307318
2214	2	-1.1321939173256368
2214	3	-0.960818858433587
2214	4	-1.5391360952933724
2214	5	-1.5335347527343046
2214	6	-2.710192004336953
2214	7	-1.452401923572637
2214	8	-2.239060387934547
2214	9	-1.5703604987138675
2214	10	-1.9400652754428407
2214	11	-1.5384835012540137
2214	12	-1.6822691752947776
2214	13	-1.9532911016884356
2214	14	-2.100861505739175
2214	15	-2.2261068617041033
2214	16	-1.7080562565443387
2214	17	-1.9481817236298085
2214	18	-1.9762740708340232
2214	19	-3.6766292020288986
2214	20	-2.218860654220663
2214	21	-2.6954713676791684
2214	22	-2.3761156595485673
2214	23	-2.334542304171123
2214	24	-1.6276518697980507
2214	25	-1.466505843706976
2215	1	-1.4754486667851587
2215	2	-1.308645101354319
2215	3	-1.3159859304778116
2215	4	-1.1620541294628453
2215	5	-1.80069980704058
2215	6	-1.7581888663407028
2215	7	-1.9052186014180172
2215	8	-2.293950975775765
2215	9	-2.0320432889844895
2215	10	-1.5973104743642708
2215	11	-2.05939851677293
2215	12	-2.772524439832477
2215	13	-2.340097832467613
2215	14	-2.257390391774372
2215	15	-3.464926516826857
2215	16	-2.7752138318534634
2215	17	-2.6035407831220496
2215	18	-3.326654107321982
2215	19	-4.069770643390775
2215	20	-3.320272195586277
2215	21	-2.523608468835789
2215	22	-3.491755276910343
2215	23	-3.5005306894476824
2215	24	-2.3955754058042444
2215	25	-2.6166237970396296
2216	1	-1.325469004623514
2216	2	-1.1057809197412602
2216	3	-1.6641520714305464
2216	4	-1.0818532069691926
2216	5	-1.3329717354513875
2216	6	-1.627445729401817
2216	7	-3.1834609517046637
2216	8	-2.144114829681012
2216	9	-2.0662630450839217
2216	10	-2.159448326535049
2216	11	-2.7123528755937976
2216	12	-2.0415280392461312
2216	13	-2.523680975853214
2216	14	-2.7284592411131996
2216	15	-2.8123083079380677
2216	16	-2.4439112124933757
2216	17	-2.765271111060102
2216	18	-2.5778195038330836
2216	19	-2.7270657876359365
2216	20	-3.026789513447504
2216	21	-2.837118165763627
2216	22	-3.381996184361668
2216	23	-2.2853763137425744
2216	24	-2.6818308644033166
2216	25	-2.458924965889736
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	49184.0
3	2.0
4	801.0
5	6998.0
6	8201.0
7	6144.0
8	4949.0
9	4316.0
10	3620.0
11	3429.0
12	3088.0
13	2861.0
14	2834.0
15	2715.0
16	2864.0
17	3007.0
18	3372.0
19	4096.0
20	5199.0
21	6462.0
22	8116.0
23	10091.0
24	11664.0
25	13382.0
26	15116.0
27	16831.0
28	19123.0
29	22795.0
30	28613.0
31	36331.0
32	46426.0
33	59462.0
34	77698.0
35	101871.0
36	138776.0
37	218425.0
38	712478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.651396042287885	19.22104755880847	13.683443269781032	27.444113129122616
2	13.840831907775538	20.255480823288217	39.384941535075946	26.5187457338603
3	16.99772472823143	24.116496322245897	30.13753957648645	28.74823937303622
4	11.625301194663571	17.10033078730268	36.75303422390218	34.521333794131564
5	12.963592095577829	37.90934948624629	35.7527765830951	13.374281835080781
6	32.71862334520465	36.773745439824964	17.40017011802239	13.107461096947995
7	27.898953017030635	31.71537092388359	21.872057091521295	18.513618967564483
8	25.38857388595569	36.47667713464177	20.171751886102648	17.962997093299894
9	25.85639484642553	15.039669516046958	19.86968526341462	39.2342503741129
10	14.455609018297514	29.1337529338391	34.5666053033567	21.844032744506677
11	34.04319356442617	22.65772007613471	24.083981734412188	19.21510462502694
12	22.705959619856692	25.01343937951905	31.422744185850515	20.857856814773747
13	28.452379519160154	21.683360822508217	25.78554831874601	24.078711339585617
14	20.883511895374163	21.982935576806156	27.470061412179135	29.663491115640543
15	23.35287492558283	30.158381404767393	23.325606187655872	23.163137481993903
16	22.303869924494272	28.109298486634486	27.19073173492203	22.39609985394921
17	21.98526817485041	28.480986745692384	27.55612338897004	21.97762169048717
18	21.955540199636314	27.584915859126564	28.817368828781433	21.642175112455693
19	23.144650756550178	27.38713446349857	27.738969271488255	21.729245508462995
20	23.325875188334717	27.22409528607915	27.8584288776485	21.591600647937632
21	22.78073456454978	26.840715134427796	27.60666402622465	22.771886274797783
22	22.977859960730353	27.44547454033927	27.03688722436307	22.539778274567304
23	22.138394308209037	27.390118819747915	27.573464793479964	22.898022078563088
24	22.7979823515957	27.124971408621956	27.845112981087556	22.231933258694788
25	22.653091690082178	26.84476901110702	27.54398383908428	22.958155459726527
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	138.0
1	138.0
2	285.5
3	433.0
4	433.0
5	433.0
6	1067.5
7	1702.0
8	1702.0
9	1702.0
10	2382.5
11	3063.0
12	3063.0
13	3063.0
14	5271.5
15	7480.0
16	7480.0
17	7480.0
18	13115.5
19	18751.0
20	18751.0
21	18751.0
22	29477.0
23	40203.0
24	40203.0
25	40203.0
26	57020.5
27	73838.0
28	73838.0
29	73838.0
30	93790.5
31	113743.0
32	113743.0
33	113743.0
34	131084.5
35	148426.0
36	148426.0
37	148426.0
38	163128.0
39	177830.0
40	177830.0
41	177830.0
42	190437.0
43	203044.0
44	203044.0
45	203044.0
46	214754.5
47	226465.0
48	226465.0
49	226465.0
50	219716.0
51	212967.0
52	212967.0
53	212967.0
54	190209.0
55	167451.0
56	167451.0
57	167451.0
58	143502.5
59	119554.0
60	119554.0
61	119554.0
62	97633.0
63	75712.0
64	75712.0
65	75712.0
66	58431.0
67	41150.0
68	41150.0
69	41150.0
70	29899.0
71	18648.0
72	18648.0
73	18648.0
74	12880.5
75	7113.0
76	7113.0
77	7113.0
78	4840.5
79	2568.0
80	2568.0
81	2568.0
82	1687.0
83	806.0
84	806.0
85	806.0
86	502.0
87	198.0
88	198.0
89	198.0
90	123.0
91	48.0
92	48.0
93	48.0
94	28.0
95	8.0
96	8.0
97	8.0
98	4.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07764816353064394
2	1.2038474965991309E-4
3	0.0
4	0.02732733817280027
5	0.07259200404492759
6	0.07921316527622281
7	0.03418926890341532
8	0.06247968507349489
9	0.04484331924831762
10	0.10985108406467067
11	0.004454235737416784
12	0.010654050344902308
13	0.004815389986396523
14	0.006380391731975393
15	0.005838660358505784
16	0.016492710703408093
17	0.026845799174160617
18	0.0011436551217691743
19	0.0029494263666678708
20	0.004153273863267002
21	1.2038474965991309E-4
22	3.6115424897973926E-4
23	0.0
24	0.0
25	0.002889233991837914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1661340.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.2886810925002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.89399533293987	59.20008912013589
2	12.413879487458859	17.947659507792864
3	3.254355390043858	7.057591768576186
4	1.1223775763812316	3.245407787375845
5	0.48364056750249224	1.7480869373791736
6	0.24882889444362613	1.079250755822081
7	0.14723794139297042	0.745054561305087
8	0.09543512857755153	0.55191036598115
9	0.05953124066734553	0.38730913844783577
>10	0.252867951071104	3.062245538681604
>50	0.013927199418009375	0.7069563719604177
>100	0.011339035157182733	1.8057085978425553
>500	0.0013338090043784273	0.6598092795827775
>1k	0.0012504459416047757	1.8029202691165893
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCAC	3877	0.23336583721574153	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3824	0.23017564134975382	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3709	0.22325351824430884	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2818	0.16962211227081753	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2329	0.14018804097896878	No Hit
GTCCTACAGTGGACATTTCTAAATT	1732	0.10425319320548473	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.019237482995654E-5	0.0
10	0.0	0.0	0.0	6.019237482995654E-5	0.0
11	0.0	0.0	0.0	1.2038474965991308E-4	0.0
12	0.0	0.0	0.0	1.2038474965991308E-4	0.0
13	0.0	0.0	0.0	1.2038474965991308E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGCCG	50	8.6123764E-5	15.221023	5
TCCAACG	95	1.0521362E-6	11.988385	18
CTGAGCG	85	5.3184922E-5	11.177427	9
TAGGACC	355	0.0	10.719029	4
GCGCCAC	120	1.5371515E-6	10.284492	13
CTAAGAC	185	5.820766E-11	10.277343	3
GGCGAGG	400	0.0	10.20323	19
GTCCTAC	960	0.0	10.201573	1
GCGTGCG	75	0.0026458537	10.134201	9
CGTGCGC	75	0.0026478383	10.133285	10
CTAGACA	160	6.031769E-9	10.10771	4
TGTAGGA	1105	0.0	9.978815	2
GTATTAT	115	1.015725E-5	9.921664	1
TTAATAC	175	2.5411282E-9	9.778157	3
ATACTGT	255	0.0	9.697623	6
AGGACGT	950	0.0	9.6132765	5
TCCTACA	1080	0.0	9.593698	2
GTAGGAC	1005	0.0	9.553838	3
AAGGCGT	120	1.6766566E-5	9.511129	6
GTCTTAG	210	6.548362E-11	9.508262	1
>>END_MODULE