FastQCFastQC Report
Thu 26 May 2016
SRR1512958_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512958_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424289
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT32770.1351736529761922No Hit
CTGTAGGACGTGGAATATGGCAAGA30460.12564508604378438No Hit
GTCCTAAAGTGTGTATTTCTCATTT30330.12510884634628958No Hit
GTACATGGGAAGCAGTGGTATCAAC27220.11228034281391369No Hit
CTTTAGGACGTGAAATATGGCGAGG26550.10951664591144043No Hit
CCCATGTACTCTGCGTTGATACCAC24360.10048306946902784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG1152.5465852E-1114.036547
TGGACCG1354.7457434E-911.257185
GGACCGT1354.76939E-911.2536976
AATACCG700.001494223510.8551395
AACCGTC700.001498086410.8517797
TAGGACC6700.010.7743524
AGACCGT803.7895775E-410.682226
GTCCTAC18200.010.2093491
TGTAGGA20950.010.1560612
GTATAAT3300.010.1062241
GTAGGAC19100.010.0454733
GGACGTG16600.010.0101136
ACCGTCC951.6525609E-49.9952668
TGGCGAG14150.09.93784218
GGCGAGG6700.09.92067419
AGGACGT16950.09.8624865
AATGTCC5850.09.7389778
GTCCTAA9800.09.62595751
TTACACA4150.09.6128814
GACGTGG8600.09.6057187
Produced by FastQC (version 0.11.5)