##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512958_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2424289
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.42999988862714	31.0	30.0	34.0	16.0	34.0
2	28.531299279912584	31.0	29.0	34.0	16.0	34.0
3	28.658325389423457	31.0	29.0	34.0	16.0	34.0
4	32.04605556515745	35.0	33.0	37.0	19.0	37.0
5	32.17188421017461	35.0	33.0	37.0	22.0	37.0
6	32.12553577564391	35.0	33.0	37.0	19.0	37.0
7	31.99817925998097	35.0	33.0	37.0	18.0	37.0
8	31.972801097558914	35.0	33.0	37.0	18.0	37.0
9	33.22900075032308	38.0	33.0	39.0	17.0	39.0
10	33.08441072825888	38.0	33.0	39.0	17.0	39.0
11	33.174364525021566	38.0	33.0	39.0	17.0	39.0
12	33.05896656710483	38.0	33.0	39.0	17.0	39.0
13	33.06342849388006	38.0	33.0	39.0	17.0	39.0
14	33.979550292889996	39.0	33.0	41.0	16.0	41.0
15	33.91680117345746	39.0	33.0	41.0	13.0	41.0
16	33.80013686487048	39.0	33.0	41.0	11.0	41.0
17	33.7133753442762	39.0	33.0	41.0	10.0	41.0
18	33.40626014472697	38.0	33.0	41.0	10.0	41.0
19	33.636483109068266	39.0	33.0	41.0	10.0	41.0
20	33.713054425441854	39.0	33.0	41.0	10.0	41.0
21	33.642013802809814	39.0	33.0	41.0	2.0	41.0
22	33.53191966799338	39.0	33.0	41.0	2.0	41.0
23	33.245678629899324	39.0	33.0	41.0	2.0	41.0
24	33.24585806395195	39.0	33.0	41.0	2.0	41.0
25	33.13886958196816	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8220134094635618
1101	2	0.9852236765542095
1101	3	1.0673586835354527
1101	4	1.1154960032884027
1101	5	0.8287021743554277
1101	6	1.0866125315510473
1101	7	1.078809534105126
1101	8	0.9004732966190261
1101	9	0.8221323293294773
1101	10	0.8276932921041009
1101	11	1.0433952120257004
1101	12	1.339715806112686
1101	13	1.1759925992433367
1101	14	1.0287261696076655
1101	15	0.931078122272126
1101	16	1.4624256466649967
1101	17	1.467660111197688
1101	18	1.52499381448645
1101	19	1.046144705970434
1101	20	1.2816143681699188
1101	21	1.1604845659188712
1101	22	1.2112192637288786
1101	23	1.4127463634181439
1101	24	1.1009952450966907
1101	25	1.2518808154305034
1102	1	0.7513468974186814
1102	2	0.8229032583286084
1102	3	0.808041075429152
1102	4	0.9400194013821697
1102	5	0.8150283427880964
1102	6	0.7296327402114713
1102	7	1.0923030613523679
1102	8	1.0151712581605707
1102	9	0.9878422677165233
1102	10	1.1918782192804755
1102	11	1.1189121328788545
1102	12	1.2206302240316802
1102	13	1.0623711499944122
1102	14	1.2165436005331927
1102	15	1.3128186298169382
1102	16	1.1749486110518461
1102	17	1.0927191804295973
1102	18	1.4331746113942074
1102	19	1.318980542513188
1102	20	1.2434628787923288
1102	21	1.0206771274860458
1102	22	1.3187312615775824
1102	23	1.4891490804053475
1102	24	1.4345142203132824
1102	25	1.3205588099380847
1103	1	0.5348717857742571
1103	2	0.7202884187920731
1103	3	0.6750606912098043
1103	4	0.81412262764972
1103	5	0.3921516081379153
1103	6	0.7724111082843734
1103	7	0.5456913162776331
1103	8	0.7684118680184469
1103	9	1.0605993727473404
1103	10	1.0241612320124958
1103	11	0.9401047258730983
1103	12	1.2168156803647605
1103	13	1.1281361897230866
1103	14	1.305867279609302
1103	15	1.3983581883056218
1103	16	1.4503012807701978
1103	17	1.2212545526749423
1103	18	1.5001029027471375
1103	19	1.5936705961806084
1103	20	1.4266027116820084
1103	21	1.2382028356780737
1103	22	1.3904722633481867
1103	23	1.6184611035097802
1103	24	1.5225388131993611
1103	25	1.3807720170866915
1104	1	0.8149928100342301
1104	2	0.8134029677655512
1104	3	0.7638355638482572
1104	4	0.9832652112017932
1104	5	0.769344497476105
1104	6	0.7863742996306584
1104	7	1.0875379521948858
1104	8	0.9507055612317643
1104	9	1.00191636591385
1104	10	1.1415818199639958
1104	11	1.1417794351017179
1104	12	1.0936325379121357
1104	13	1.106313144595056
1104	14	1.351393120533018
1104	15	1.4558532858064623
1104	16	1.2587110419185592
1104	17	1.252380403833044
1104	18	1.4687032274981533
1104	19	1.2906402807779855
1104	20	1.2687554399267071
1104	21	1.3741624850035947
1104	22	1.4135817509310442
1104	23	1.6521388808747304
1104	24	1.4864969894274793
1104	25	1.192177920064502
1105	1	0.6358188765362414
1105	2	0.7505935486841651
1105	3	0.8326586231552149
1105	4	0.9883039944876195
1105	5	0.6601149132812196
1105	6	0.45591695333713744
1105	7	0.7161708366827284
1105	8	0.8358545292570696
1105	9	0.7651099808872175
1105	10	0.8825790331684118
1105	11	0.8050298985507567
1105	12	0.7603136797272967
1105	13	0.8317079429084018
1105	14	0.8184140852064559
1105	15	1.0237483562957763
1105	16	0.9036436707825501
1105	17	0.8626041035051131
1105	18	0.9108572959340648
1105	19	0.9880842263335197
1105	20	1.020903925772771
1105	21	0.994061264857649
1105	22	0.6879362848577344
1105	23	0.9538378111021757
1105	24	1.11822507878491
1105	25	1.1135636158045585
1106	1	0.795044169681109
1106	2	0.8540758239281239
1106	3	0.851587828923666
1106	4	0.8864059174487444
1106	5	0.8862560466449594
1106	6	0.865147951857729
1106	7	0.6896982259793063
1106	8	0.8073845380363593
1106	9	1.0000805075482901
1106	10	1.0909319028393298
1106	11	1.0355291405508353
1106	12	1.0661961383417022
1106	13	1.0502370737584528
1106	14	1.3415163990606729
1106	15	1.4503477161625966
1106	16	1.255763093112428
1106	17	1.2934047490276228
1106	18	1.654376138810683
1106	19	1.235720554040988
1106	20	1.229885218461213
1106	21	1.0873509778489705
1106	22	1.1767323574301116
1106	23	1.5202939277806067
1106	24	1.377020456066603
1106	25	1.4664899745406217
1107	1	0.5522126332148716
1107	2	0.5304551653747787
1107	3	0.6443354068280094
1107	4	0.7665331926701207
1107	5	0.7292039366806904
1107	6	0.6022041410720789
1107	7	0.8686159020639366
1107	8	0.7677460787619097
1107	9	0.9440397962397
1107	10	0.665365808675439
1107	11	0.7784749025668631
1107	12	1.0015637275540143
1107	13	0.9256365439797634
1107	14	1.0230938051225138
1107	15	1.1356780322913806
1107	16	1.0617203696793567
1107	17	1.167614427064791
1107	18	1.4938870478581379
1107	19	1.306291318389654
1107	20	1.198173010536003
1107	21	1.1525571027480126
1107	22	1.127497904332813
1107	23	1.1892629153310352
1107	24	1.266700509941515
1107	25	1.2228534573296912
1108	1	0.2959812938537354
1108	2	0.38258758919327107
1108	3	0.29589309165854516
1108	4	0.4229380777351075
1108	5	0.32937336408413387
1108	6	0.38693900482617494
1108	7	0.45865168734021466
1108	8	0.49795615339692034
1108	9	0.6074026416547653
1108	10	0.4827968299306562
1108	11	0.34966480743215556
1108	12	0.4536027420237474
1108	13	0.34504392973184395
1108	14	0.5266386584306844
1108	15	0.5002114259040411
1108	16	0.41488738531952407
1108	17	0.4982388630527339
1108	18	0.6359496566479947
1108	19	0.4863834804442462
1108	20	0.5384724358953861
1108	21	0.4009900992791344
1108	22	0.42099684580500707
1108	23	0.5441196801214545
1108	24	0.5482343460091599
1108	25	0.7267217414916303
1109	1	0.10977520809857211
1109	2	-0.026409849652143436
1109	3	0.17926371427295962
1109	4	0.2609067429167702
1109	5	0.16109181375641413
1109	6	0.10749388771878188
1109	7	0.3086448074596433
1109	8	0.3308286496704582
1109	9	0.32521719751168376
1109	10	0.5491261689925153
1109	11	0.10155936891119666
1109	12	0.39206042057799095
1109	13	0.3209259911739366
1109	14	0.2109583433288691
1109	15	0.5082144182415931
1109	16	0.479911024851873
1109	17	0.3406418493478114
1109	18	-1.3551481391302218
1109	19	0.10173799461466615
1109	20	0.35660103613360405
1109	21	0.4789772728173034
1109	22	0.6287904533286763
1109	23	0.16182997048525039
1109	24	0.6645472817280833
1109	25	0.6690111358683879
1110	1	0.3086883057572969
1110	2	0.29568042085642077
1110	3	0.5256400699068884
1110	4	0.28502270419741293
1110	5	0.47721985201744843
1110	6	0.35977971649315066
1110	7	0.5962458346762176
1110	8	0.4037592788891615
1110	9	0.6350555733856851
1110	10	0.9707100168080629
1110	11	0.7856227523430235
1110	12	0.17961885209828665
1110	13	0.5471104978633434
1110	14	0.6902393564590952
1110	15	0.7409940722583741
1110	16	0.9834122875100135
1110	17	0.5164454223369646
1110	18	0.9244839324512597
1110	19	0.6194442746315261
1110	20	0.7546524448665011
1110	21	0.7913019944401896
1110	22	0.9186501529448634
1110	23	0.8233331713123064
1110	24	1.0376764535007368
1110	25	1.019291082811499
1111	1	0.16303684959888898
1111	2	0.4381559145538958
1111	3	0.41391292382382616
1111	4	0.3243685881028391
1111	5	0.3333479742766343
1111	6	0.5706441937678761
1111	7	0.4466602715935224
1111	8	0.25157709575233156
1111	9	0.6092026822995535
1111	10	0.6070884988808345
1111	11	0.4427129233215794
1111	12	0.4176767412262379
1111	13	0.6338450605053296
1111	14	0.4676454378923154
1111	15	0.7298135024951691
1111	16	0.6848131828261899
1111	17	0.5370215751947143
1111	18	0.8153893515056367
1111	19	0.5889077062143144
1111	20	0.4581790466684481
1111	21	0.7199872129909153
1111	22	0.33935985003135727
1111	23	0.740644771918511
1111	24	0.7893192097618069
1111	25	0.3220410201879105
1112	1	-0.2932041857647292
1112	2	0.0706320166045522
1112	3	0.12555463231859676
1112	4	0.270330833124401
1112	5	0.245151480590593
1112	6	0.20842062832234376
1112	7	-0.10038799299002221
1112	8	0.2625096909180016
1112	9	0.5090648132555629
1112	10	0.3742862428107614
1112	11	0.4357590062918604
1112	12	0.49190130945163446
1112	13	0.11868510333579252
1112	14	0.33843130463060334
1112	15	0.3970036042508198
1112	16	0.48637914939540394
1112	17	0.5320308859213654
1112	18	0.5437767848171191
1112	19	0.43865510894654847
1112	20	0.35298804539129947
1112	21	0.4319944139104024
1112	22	0.4204449016285636
1112	23	0.6531813730868805
1112	24	0.21206352047956045
1112	25	0.529980823855901
1113	1	-0.1780396262354813
1113	2	-0.06260088023386956
1113	3	-0.20920604450616054
1113	4	0.11870935093250878
1113	5	-0.14679967069917055
1113	6	0.09511251685777466
1113	7	-0.025122664161624897
1113	8	0.14989095344866854
1113	9	0.12547021990801
1113	10	-0.30222562904622663
1113	11	-0.10963859788260066
1113	12	0.09590586982549354
1113	13	-0.11648129921638883
1113	14	0.009528156137434962
1113	15	-0.39814804629743605
1113	16	-0.36855643734411103
1113	17	-0.36077751868030106
1113	18	-0.399974455052444
1113	19	-0.25680164401200045
1113	20	-0.4145662918516564
1113	21	-0.26051526195999486
1113	22	-0.007341901545188989
1113	23	-0.268385032176397
1113	24	0.3076447882760007
1113	25	0.2194441679024024
1114	1	-1.333084336347003
1114	2	-1.021627355272102
1114	3	-1.0520022100595199
1114	4	-1.166304541244262
1114	5	-1.12360415043187
1114	6	-1.574651291760084
1114	7	-1.7833072391596083
1114	8	-1.20653587792701
1114	9	-1.4534223929425636
1114	10	-1.5162097070786018
1114	11	-2.1605262454108036
1114	12	-1.5948775416857544
1114	13	-1.670175009356619
1114	14	-1.9239208956788403
1114	15	-1.8597634932929168
1114	16	-2.595658451804539
1114	17	-4.566968678716336
1114	18	-3.610021648908578
1114	19	-2.424240429322751
1114	20	-1.5230857525459243
1114	21	-2.6701360751439402
1114	22	-2.695473908914625
1114	23	-2.712066732585331
1114	24	-2.232736646640408
1114	25	-2.083935214471296
1115	1	-1.3380031749123482
1115	2	-0.8319903028536508
1115	3	-0.9665175704357978
1115	4	-1.0330381805416025
1115	5	-0.6774156519939503
1115	6	-0.845857837207884
1115	7	-2.218852115259743
1115	8	-1.4641957229785092
1115	9	-1.084910079840924
1115	10	-0.8665019444860107
1115	11	-1.9399629154390645
1115	12	-1.3236855056449848
1115	13	-1.0084341617260364
1115	14	-1.232987569844525
1115	15	-2.754686517149075
1115	16	-1.3030146314208295
1115	17	-1.2802352727661628
1115	18	-1.4638432621811255
1115	19	-1.507212548225695
1115	20	-1.3878832087028314
1115	21	-2.005342268776019
1115	22	-2.187167198598079
1115	23	-1.4169943067111994
1115	24	-1.3301652207187082
1115	25	-1.784100711460372
1116	1	-1.873764437425283
1116	2	-2.700556850226512
1116	3	-2.1900023342940997
1116	4	-1.9141946346251224
1116	5	-2.702496150877579
1116	6	-2.1831900497472816
1116	7	-1.7181803021567852
1116	8	-2.6870236843669737
1116	9	-2.655314312699833
1116	10	-3.6496752691248098
1116	11	-2.280031426761269
1116	12	-2.343672338177985
1116	13	-2.966001720047583
1116	14	-2.728145767954164
1116	15	-2.3429088951405532
1116	16	-2.291362016568538
1116	17	-2.7452625921184293
1116	18	-2.5834357688730734
1116	19	-2.896359371766277
1116	20	-3.2678246742153796
1116	21	-3.621601018578783
1116	22	-2.807241965514365
1116	23	-2.8222733718350064
1116	24	-3.4768973011645308
1116	25	-3.127990301621214
1201	1	0.20180919059126623
1201	2	0.1940931789697835
1201	3	0.41633095032484846
1201	4	0.49033630407300066
1201	5	0.5702686349612804
1201	6	0.5548810159143187
1201	7	0.48941773499849006
1201	8	-0.0045014023870031394
1201	9	0.297046820595007
1201	10	0.5669611719921122
1201	11	0.15728472225042367
1201	12	-0.4576554243720494
1201	13	0.004728830789218819
1201	14	0.5342409953566616
1201	15	0.26807276001464686
1201	16	0.7607555555928727
1201	17	0.7861905169476842
1201	18	1.0374360913933813
1201	19	0.5010271231843433
1201	20	0.6008556855036886
1201	21	0.5479898582089646
1201	22	0.6431523784872013
1201	23	1.0184016607723763
1201	24	0.7695472494743925
1201	25	0.6356234013033912
1202	1	0.5676156449563052
1202	2	0.8051956132067986
1202	3	0.4288527575646732
1202	4	0.8902473687892076
1202	5	0.7977344183389832
1202	6	0.7444996276211882
1202	7	0.7985507542763841
1202	8	0.8036365132165315
1202	9	1.2113438520582491
1202	10	1.203956529963854
1202	11	0.9050183380263235
1202	12	0.9807140614821108
1202	13	1.068638044968992
1202	14	1.3054709057938894
1202	15	0.9216503582858664
1202	16	1.0674695158866996
1202	17	1.322647515781604
1202	18	1.6277516403303594
1202	19	1.3622267078319368
1202	20	1.2421982491351429
1202	21	1.225101937032349
1202	22	1.4112069428914609
1202	23	1.5452495069918086
1202	24	1.6449068504806803
1202	25	1.469718571827137
1203	1	0.16337791705799276
1203	2	0.46303081167176074
1203	3	0.5751646131315731
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2211	17	0.2937217297125372
2211	18	0.060517927595554966
2211	19	0.009236373450875135
2211	20	-0.03255635571161264
2211	21	-0.003003987852601142
2211	22	-0.10771583943365215
2211	23	0.4605310543490475
2211	24	0.12137734406017842
2211	25	0.19173091936031028
2212	1	-0.17671526244703273
2212	2	-0.23411940244648832
2212	3	-0.1484205331059023
2212	4	-0.4201934284475719
2212	5	-0.3115175308210425
2212	6	-0.40229721976166744
2212	7	-0.4500226044474154
2212	8	-0.29699256634074445
2212	9	-0.2866333531267884
2212	10	-0.1982815614433875
2212	11	-0.4003624914053674
2212	12	-0.18373482667188767
2212	13	-0.30074084951863966
2212	14	-0.3339642970332619
2212	15	-0.567757107023894
2212	16	-0.25346816942860784
2212	17	-0.2702235098723875
2212	18	-3.0869303897152562
2212	19	-1.3349634567614643
2212	20	-0.5495564102305863
2212	21	-0.6496110903768297
2212	22	-0.36211065286193644
2212	23	-0.39599720335435507
2212	24	-0.7875646763091453
2212	25	-0.3766970779500909
2213	1	0.14070932160127114
2213	2	0.30333775626944615
2213	3	-0.13783864646913102
2213	4	-0.10228100523978156
2213	5	-2.835001191243691E-4
2213	6	0.06866453981697873
2213	7	0.17000652144398032
2213	8	-0.20157325696794715
2213	9	-0.039798906749730634
2213	10	-0.12379677933121513
2213	11	-0.16055033908919114
2213	12	-0.1940640416458237
2213	13	0.07323448977911795
2213	14	-0.04703092934231279
2213	15	-0.08986434834778123
2213	16	-0.565909662241161
2213	17	-0.2009817786387771
2213	18	-0.09606788982100056
2213	19	0.08282917027550951
2213	20	-0.6782528313769234
2213	21	-0.40073301632964586
2213	22	-0.3548634725618669
2213	23	-0.81949638378196
2213	24	-0.48536630920708745
2213	25	-0.6070643335766377
2214	1	-1.5130247916188324
2214	2	-3.6562594444433145
2214	3	-1.7717938698190885
2214	4	-1.2565731556026343
2214	5	-1.2266171932470478
2214	6	-1.4273477131086594
2214	7	-1.6491028376239107
2214	8	-1.2908135499801112
2214	9	-1.4396899728936283
2214	10	-1.3693139236145306
2214	11	-1.1488991094591796
2214	12	-1.7311507578304663
2214	13	-1.4905627794794896
2214	14	-2.789484415581235
2214	15	-2.8771727008440884
2214	16	-2.0657037402781917
2214	17	-2.649966603270908
2214	18	-2.084028849974814
2214	19	-4.150702690033178
2214	20	-2.4884165308048054
2214	21	-2.410158354685887
2214	22	-2.410398549690168
2214	23	-2.604733713110626
2214	24	-2.778074851413269
2214	25	-2.5701033321815423
2215	1	-0.9835129760552128
2215	2	-2.761342000376775
2215	3	-1.3830512394365222
2215	4	-0.9871644059302191
2215	5	-0.8573706558702412
2215	6	-1.4416789920863735
2215	7	-2.0079838243936514
2215	8	-1.982378510608541
2215	9	-1.7365250597375308
2215	10	-3.343420433523473
2215	11	-2.2249319169035466
2215	12	-2.4045756638596494
2215	13	-1.6572998765766265
2215	14	-3.3181463672488825
2215	15	-2.739751588357546
2215	16	-2.391497177728315
2215	17	-2.8691849558445313
2215	18	-1.7227591651368428
2215	19	-2.4205287930563237
2215	20	-2.614145307663165
2215	21	-2.345020614438667
2215	22	-1.9146250575922963
2215	23	-2.5877432559287143
2215	24	-2.3360004195844937
2215	25	-2.1579542799684965
2216	1	-2.3962581680246657
2216	2	-2.518836037071914
2216	3	-2.9249083011314028
2216	4	-3.6921099451448463
2216	5	-2.775044258130624
2216	6	-2.8955869851952087
2216	7	-2.8050907509485725
2216	8	-3.4994666006504644
2216	9	-3.723242366031325
2216	10	-3.218588850262936
2216	11	-3.7920221458513197
2216	12	-4.025076935006091
2216	13	-4.569061171855587
2216	14	-4.097257317775107
2216	15	-3.899359394338635
2216	16	-3.100705149730924
2216	17	-3.2500513470578376
2216	18	-3.4434664959980026
2216	19	-3.8420360747049713
2216	20	-3.656685228307989
2216	21	-4.032447630905416
2216	22	-4.596690933064135
2216	23	-4.484639862634129
2216	24	-4.347262731108884
2216	25	-4.3960723044396985
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	147145.0
3	1.0
4	1188.0
5	6849.0
6	8320.0
7	7392.0
8	6515.0
9	6207.0
10	5771.0
11	5481.0
12	5275.0
13	5257.0
14	5216.0
15	5429.0
16	5587.0
17	5771.0
18	6557.0
19	8002.0
20	9902.0
21	12996.0
22	16359.0
23	19232.0
24	22390.0
25	25059.0
26	28220.0
27	31222.0
28	35142.0
29	41660.0
30	50496.0
31	62751.0
32	78429.0
33	98788.0
34	125588.0
35	161191.0
36	215667.0
37	326812.0
38	820422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.679634056733626	17.99517733138771	12.369627536816832	25.955561075061834
2	15.181226027552711	19.424723162722753	40.77103849828028	24.62301231144426
3	18.8969554822529	23.660188542062972	29.128494312625286	28.314361663058847
4	12.666580288645637	16.184448985250814	37.25679360384509	33.89217712225846
5	13.656867223675473	37.362878156696176	35.566733146233176	13.413521473395173
6	33.15522715036296	36.290630344062194	17.533454216149202	13.020688289425642
7	29.112546388073497	31.009037294051396	21.46081948309401	18.4175968347811
8	25.925244380042944	36.18772084149584	19.981193918019017	17.905840860442204
9	26.79266628269089	14.954797579302276	19.4643537902205	38.78818234778634
10	15.216715034602945	28.383864555475697	34.167676796015485	22.23174361390587
11	35.05061490424921	21.963724610032546	24.048350683557004	18.93730980216124
12	23.673697399977804	24.17629932003101	31.09324375450483	21.056759525486356
13	29.21972419984619	21.344971334079275	25.313229231647703	24.12207523442683
14	21.312657101784627	21.473740586271266	26.979585639143288	30.234016672800823
15	23.57448869827927	30.080926105260463	22.779545372960293	23.56503982349997
16	23.059550160804207	27.54688489554431	26.743833432009424	22.64973151164206
17	22.477074440301127	28.102408122410484	27.117762244225187	22.30275519306321
18	22.52773363489494	27.00962280439907	28.546744732477613	21.91589882822838
19	23.976183039554787	26.74382574058724	26.886709348829513	22.39328187102846
20	24.1580242471853	26.812350022940095	27.1481170968072	21.881508633067405
21	23.54063704548474	26.457630013871775	26.480158242237046	23.521574698406436
22	23.80826839355322	27.36646499853122	26.290255237265363	22.53501137065019
23	23.03662286094337	27.18609153157197	26.831514375351727	22.945771232132923
24	23.59529237804551	26.47781554195165	27.33666857275352	22.59022350724932
25	23.341972930911275	26.67321031602608	27.047345324797707	22.937471428264935
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	139.0
1	139.0
2	281.5
3	424.0
4	424.0
5	424.0
6	737.0
7	1050.0
8	1050.0
9	1050.0
10	1737.0
11	2424.0
12	2424.0
13	2424.0
14	4770.0
15	7116.0
16	7116.0
17	7116.0
18	14109.5
19	21103.0
20	21103.0
21	21103.0
22	35004.5
23	48906.0
24	48906.0
25	48906.0
26	71528.0
27	94150.0
28	94150.0
29	94150.0
30	124414.0
31	154678.0
32	154678.0
33	154678.0
34	181661.0
35	208644.0
36	208644.0
37	208644.0
38	233222.5
39	257801.0
40	257801.0
41	257801.0
42	276727.5
43	295654.0
44	295654.0
45	295654.0
46	311090.0
47	326526.0
48	326526.0
49	326526.0
50	319865.5
51	313205.0
52	313205.0
53	313205.0
54	284994.5
55	256784.0
56	256784.0
57	256784.0
58	223613.0
59	190442.0
60	190442.0
61	190442.0
62	156770.5
63	123099.0
64	123099.0
65	123099.0
66	96211.0
67	69323.0
68	69323.0
69	69323.0
70	50981.5
71	32640.0
72	32640.0
73	32640.0
74	22889.5
75	13139.0
76	13139.0
77	13139.0
78	9020.0
79	4901.0
80	4901.0
81	4901.0
82	3229.0
83	1557.0
84	1557.0
85	1557.0
86	1002.0
87	447.0
88	447.0
89	447.0
90	278.0
91	109.0
92	109.0
93	109.0
94	62.5
95	16.0
96	16.0
97	16.0
98	14.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35532067340156226
2	0.038526759804627254
3	0.03510307558216038
4	0.03436058984716756
5	0.0407542170096057
6	0.018727140204818814
7	0.018314648129822805
8	0.024955770537258552
9	0.02165583393729048
10	0.020913348202297663
11	0.020500856127301653
12	0.020707102164799658
13	0.02083084978729846
14	0.02891569445722024
15	0.029699429399712658
16	0.02202707680478689
17	0.023718294312270526
18	0.09198573272411004
19	0.02631699438474538
20	0.024130786387266533
21	0.027348224572235406
22	0.02083084978729846
23	0.02297580857727771
24	0.019593373562310435
25	0.023305802237274516
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2424289.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.93098335913824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.65230053091148	57.91677970193436
2	12.068071280375339	17.120003263303946
3	3.32392838662411	7.073085272358058
4	1.2613467535105778	3.5787426233344735
5	0.5940290902653951	2.106753375822938
6	0.3170804610626785	1.3494497344286838
7	0.18999189398602934	0.9433418309485943
8	0.12910225443047474	0.7325879888508188
9	0.08717802833156287	0.5565260949181711
>10	0.3466196401170219	4.094627119821272
>50	0.016524873334830055	0.8077894255744872
>100	0.010972614163622581	1.5317387804070925
>500	0.0019804603706021005	0.947014833074031
>1k	8.737325164341051E-4	1.2415599552232939
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3277	0.1351736529761922	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3046	0.12564508604378438	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3033	0.12510884634628958	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2722	0.11228034281391369	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2655	0.10951664591144043	No Hit
CCCATGTACTCTGCGTTGATACCAC	2436	0.10048306946902784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	4.1249207499600914E-5
13	0.0	0.0	0.0	0.0	8.249841499920183E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTG	115	2.5465852E-11	14.03654	7
TGGACCG	135	4.7457434E-9	11.25718	5
GGACCGT	135	4.76939E-9	11.253697	6
AATACCG	70	0.0014942235	10.855139	5
AACCGTC	70	0.0014980864	10.851779	7
TAGGACC	670	0.0	10.774352	4
AGACCGT	80	3.7895775E-4	10.68222	6
GTCCTAC	1820	0.0	10.209349	1
TGTAGGA	2095	0.0	10.156061	2
GTATAAT	330	0.0	10.106224	1
GTAGGAC	1910	0.0	10.045473	3
GGACGTG	1660	0.0	10.010113	6
ACCGTCC	95	1.6525609E-4	9.995266	8
TGGCGAG	1415	0.0	9.937842	18
GGCGAGG	670	0.0	9.920674	19
AGGACGT	1695	0.0	9.862486	5
AATGTCC	585	0.0	9.738977	8
GTCCTAA	980	0.0	9.6259575	1
TTACACA	415	0.0	9.612881	4
GACGTGG	860	0.0	9.605718	7
>>END_MODULE