FastQCFastQC Report
Thu 26 May 2016
SRR1512958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424289
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA32110.13245120528121854No Hit
GTCCTACAGTGGACATTTCTAAATT30900.12746005117376683No Hit
CTTTAGGACGTGAAATATGGCGAGG29040.11978769857884106No Hit
GTCCTAAAGTGTGTATTTCTCATTT28910.11925145888134624No Hit
CCCATGTACTCTGCGTTGATACCAC27200.1121978443989145No Hit
GTACATGGGAAGCAGTGGTATCAAC26520.10939289828894162No Hit
GTATCAACGCAGAGTACTTTTTTTT26370.1087741601764476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5850.013.6618254
TGAACCG801.9667768E-613.0824185
CTACACT3700.011.57167154
CCTACAC3500.011.4086533
CGAATGC600.00591372811.07412714
ACCGCTA700.001490706710.85820510
GGCGAGG6800.010.606420519
GAACCGT1002.3663442E-510.4633386
GTAGGAC19350.010.3179043
TGTAGGA21100.010.2729842
GTCCTAC17350.010.192381
ACCGTCC856.569856E-410.0631338
GTCTAGG1803.8926373E-1010.0355981
GGACGTG16800.010.0217036
AGGACGT17100.010.0152485
TGGCGAG17050.09.90740318
GTCCTAA9250.09.8671471
TCTACAC3000.09.8241193
TAGGACA6500.09.8072394
CTGTAGG21700.09.7264731
Produced by FastQC (version 0.11.5)