##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512958_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2424289 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.514434541426372 31.0 31.0 34.0 27.0 34.0 2 30.63578063506455 31.0 31.0 34.0 27.0 34.0 3 30.745690798415534 31.0 31.0 34.0 27.0 34.0 4 34.253436780845846 37.0 35.0 37.0 30.0 37.0 5 33.92696539067743 37.0 35.0 37.0 30.0 37.0 6 33.80367480939773 37.0 35.0 37.0 29.0 37.0 7 33.67607781085506 37.0 35.0 37.0 28.0 37.0 8 33.55422558944086 37.0 35.0 37.0 28.0 37.0 9 35.02168223343009 39.0 35.0 39.0 28.0 39.0 10 34.90335104436806 39.0 35.0 39.0 27.0 39.0 11 34.96109374748637 39.0 35.0 39.0 27.0 39.0 12 34.806811811628066 39.0 35.0 39.0 27.0 39.0 13 34.80849849172273 39.0 35.0 39.0 27.0 39.0 14 35.86742545958836 40.0 36.0 41.0 27.0 41.0 15 35.87810776685453 40.0 36.0 41.0 27.0 41.0 16 35.84894787708891 40.0 36.0 41.0 27.0 41.0 17 35.81445240233322 40.0 36.0 41.0 27.0 41.0 18 35.73292994358346 40.0 36.0 41.0 27.0 41.0 19 35.70991659822736 40.0 36.0 41.0 27.0 41.0 20 35.681968610178075 40.0 36.0 41.0 27.0 41.0 21 35.62767722825125 40.0 36.0 41.0 27.0 41.0 22 35.593502259837834 40.0 36.0 41.0 27.0 41.0 23 35.48333016401922 40.0 36.0 41.0 27.0 41.0 24 35.46773301367948 40.0 36.0 41.0 27.0 41.0 25 35.36981193248825 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6966973637436595 1101 2 0.7329194784287054 1101 3 0.7298848085394027 1101 4 0.5377508326510068 1101 5 0.8648119111103654 1101 6 0.9065839524074804 1101 7 0.9618691429045114 1101 8 0.92109858322398 1101 9 0.6734741450462494 1101 10 1.016000054173908 1101 11 0.9871328407250388 1101 12 1.1643941136873863 1101 13 1.1038992743848794 1101 14 1.2580042622278853 1101 15 1.2357131753623705 1101 16 1.3680469959771102 1101 17 1.3841650600734496 1101 18 0.7882949266584234 1101 19 1.1444079849542987 1101 20 1.0427080563488502 1101 21 1.2694311365158768 1101 22 1.1353775267578996 1101 23 1.1124607396561856 1101 24 1.099307799344352 1101 25 1.1699315191594621 1102 1 0.6721289434242017 1102 2 0.5624676297708824 1102 3 0.520650513092356 1102 4 0.42765418565792856 1102 5 0.7319028542110999 1102 6 0.6396892545105644 1102 7 0.6286615893670202 1102 8 0.6643613159151016 1102 9 0.7650253689578648 1102 10 0.9503152837136568 1102 11 0.9069073992900201 1102 12 0.8801247859941554 1102 13 0.9713429992954872 1102 14 1.0626880098147637 1102 15 1.0560672424957502 1102 16 1.0467160470744616 1102 17 1.0433178739087055 1102 18 1.0356534970914595 1102 19 1.0933815033407086 1102 20 0.9629881994290557 1102 21 1.0090110942294501 1102 22 1.1070342046418276 1102 23 1.1017222566754086 1102 24 1.016582655005756 1102 25 1.056944402193949 1103 1 0.6158996419836384 1103 2 0.5751304605080421 1103 3 0.5838811930226591 1103 4 0.6302790696397551 1103 5 0.652358574005298 1103 6 0.751140446439905 1103 7 0.6624680464276338 1103 8 0.7389435297687967 1103 9 0.8321350950025206 1103 10 0.9456129518156615 1103 11 0.8276346502426222 1103 12 0.9624817178410794 1103 13 0.946918716407481 1103 14 1.07638632903965 1103 15 0.9100926160339071 1103 16 1.0363561261772318 1103 17 1.195216170588779 1103 18 1.1892888067383893 1103 19 1.200569738866946 1103 20 1.3050969010230702 1103 21 0.8602942514193614 1103 22 1.0252289584335657 1103 23 1.126099076799953 1103 24 1.1840414945108293 1103 25 1.1834130056449013 1104 1 0.5092715611053649 1104 2 0.6097574357466975 1104 3 0.6177374595999368 1104 4 0.529226684178731 1104 5 0.5752184352722907 1104 6 0.6214997779552718 1104 7 0.7203932736696501 1104 8 0.6518292260393608 1104 9 0.8865255145345046 1104 10 0.9230312424755809 1104 11 0.8900357853877381 1104 12 0.9532501577909258 1104 13 0.9760277687570138 1104 14 1.145006687044237 1104 15 1.1388912890486154 1104 16 1.0556689583607621 1104 17 1.029955097963196 1104 18 1.0177137888044072 1104 19 1.0948660864863555 1104 20 1.090387375714549 1104 21 0.9716673009500809 1104 22 1.0578752682116317 1104 23 0.927898748891522 1104 24 1.0029071305809225 1104 25 1.069162285393638 1105 1 0.586131668248278 1105 2 0.6976120203591343 1105 3 0.6451112379525092 1105 4 0.5638154264626181 1105 5 0.547568083870452 1105 6 0.5751935961147296 1105 7 0.6398197114762638 1105 8 0.7347023598615152 1105 9 0.8320181953017851 1105 10 0.8251441528605241 1105 11 0.8143920679214602 1105 12 0.8387420300404926 1105 13 0.9174379122730016 1105 14 1.0712327860914783 1105 15 0.8869373996455181 1105 16 0.9675419591797123 1105 17 0.6824759996117891 1105 18 0.9428692495132793 1105 19 1.001275593618459 1105 20 0.9759295587628287 1105 21 0.8420488224617984 1105 22 0.960068144134361 1105 23 0.9022984602793329 1105 24 0.9637153592460947 1105 25 1.069447243576029 1106 1 0.5280057660753457 1106 2 0.5284140631165144 1106 3 0.5774120147152964 1106 4 0.4947425217410597 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2216 18 -2.5067233212948707 2216 19 -2.638549243093877 2216 20 -3.0759136282299337 2216 21 -2.853361868638025 2216 22 -3.0558836057095604 2216 23 -2.088164346111803 2216 24 -2.5561191645471055 2216 25 -2.2836981820118467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56208.0 3 3.0 4 882.0 5 7778.0 6 8836.0 7 7215.0 8 5647.0 9 5171.0 10 4537.0 11 4091.0 12 3913.0 13 3749.0 14 3665.0 15 3607.0 16 3744.0 17 4053.0 18 4425.0 19 5433.0 20 6698.0 21 8934.0 22 11437.0 23 13965.0 24 16707.0 25 19813.0 26 22292.0 27 25255.0 28 28340.0 29 34509.0 30 43716.0 31 55338.0 32 70467.0 33 91058.0 34 117881.0 35 153641.0 36 209240.0 37 322465.0 38 1039576.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.98015164506452 18.921121366002758 13.33468462871233 27.764042360220397 2 14.224353067253617 19.9063559332521 39.23542849317283 26.63386250632145 3 17.257678436853034 23.6381058528913 29.738987389704775 29.36522832055089 4 11.853315514486203 16.723942514825772 36.51470446858892 34.908037502099106 5 13.282029774941858 37.553775447107085 35.62566097052144 13.538533807429618 6 33.29546570984869 36.51180569946261 17.16971988141049 13.0230087092782 7 28.87608894434706 31.070978209559275 21.517771373679153 18.53516147241451 8 25.748547978521103 36.59420215077908 19.755499849958124 17.901750020741687 9 26.411420619480786 14.75321547455642 19.37076322073223 39.464600685230565 10 14.842211149900027 28.75724608057138 34.18019254608627 22.220350223442324 11 34.99598839211869 21.89578894611245 24.00748283464957 19.100739827119295 12 23.400870647176667 24.27254920080662 31.129720818632773 21.196859333383937 13 29.083781533889574 21.22333794528199 25.446076089727566 24.24680443110087 14 21.12450677265855 21.397063221792965 27.018937042224177 30.45949296332431 15 23.43884328204282 30.159935646219218 22.6977022400066 23.703518831731365 16 22.938445251433066 27.62657055415694 26.631779673953492 22.803204520456504 17 22.37713704749306 28.139459653054978 27.143030442069787 22.340372857382178 18 22.445964168197367 27.096228103211235 28.463071991538904 21.994735737052498 19 23.886933607897685 26.765651735580008 26.900293869657833 22.44712078686447 20 24.091073534610675 26.804518365398856 27.126899273188844 21.977508826801632 21 23.402366632031082 26.384973078704725 26.58181429689282 23.63084599237137 22 23.746361295277822 27.211674544597308 26.413294157489037 22.628670002635833 23 22.85354592624889 27.033163125353454 26.95016971986426 23.1631212285334 24 23.518648147972456 26.47803129082382 27.355360685132833 22.647959876070882 25 23.210460466963916 26.624434404072577 27.078973671042935 23.08613145792057 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 80.0 1 80.0 2 164.5 3 249.0 4 249.0 5 249.0 6 650.5 7 1052.0 8 1052.0 9 1052.0 10 1652.5 11 2253.0 12 2253.0 13 2253.0 14 4531.0 15 6809.0 16 6809.0 17 6809.0 18 13860.0 19 20911.0 20 20911.0 21 20911.0 22 34929.0 23 48947.0 24 48947.0 25 48947.0 26 72095.5 27 95244.0 28 95244.0 29 95244.0 30 124865.0 31 154486.0 32 154486.0 33 154486.0 34 182091.5 35 209697.0 36 209697.0 37 209697.0 38 233716.5 39 257736.0 40 257736.0 41 257736.0 42 277950.5 43 298165.0 44 298165.0 45 298165.0 46 313354.5 47 328544.0 48 328544.0 49 328544.0 50 321488.5 51 314433.0 52 314433.0 53 314433.0 54 285438.0 55 256443.0 56 256443.0 57 256443.0 58 222720.0 59 188997.0 60 188997.0 61 188997.0 62 155676.0 63 122355.0 64 122355.0 65 122355.0 66 95068.5 67 67782.0 68 67782.0 69 67782.0 70 49395.5 71 31009.0 72 31009.0 73 31009.0 74 21753.5 75 12498.0 76 12498.0 77 12498.0 78 8569.5 79 4641.0 80 4641.0 81 4641.0 82 3047.0 83 1453.0 84 1453.0 85 1453.0 86 928.0 87 403.0 88 403.0 89 403.0 90 246.5 91 90.0 92 90.0 93 90.0 94 51.0 95 12.0 96 12.0 97 12.0 98 6.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07928097681423296 2 1.2374762249880276E-4 3 0.0 4 0.02631699438474538 5 0.07074239086181557 6 0.07486731161177566 7 0.03469058350716437 8 0.06731870663934869 9 0.047972828322035865 10 0.10869166176144841 11 0.0030524413549704674 12 0.014230976587362314 13 0.006228630332439739 14 0.008414838329918586 15 0.0077961002174245725 16 0.019098383072315223 17 0.030318167512206673 18 7.424857349928165E-4 19 0.0035061826374660782 20 0.005692390634944926 21 0.0 22 2.474952449976055E-4 23 0.0 24 0.0 25 0.0031349397699696694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2424289.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.58059586190723 #Duplication Level Percentage of deduplicated Percentage of total 1 79.47894590678276 54.507134687634526 2 13.430367082879771 18.42125154376079 3 3.7949476690731414 7.807793172293757 4 1.4377153735426504 3.94397507989518 5 0.6333375568305288 2.17173335145811 6 0.34487785653548686 1.4191157340468625 7 0.21225279571902303 1.0189496242636389 8 0.14054641815861657 0.7711005682859761 9 0.09294687556181802 0.5736916898578821 >10 0.40121656591317245 4.587257386091545 >50 0.0176612142176038 0.8232184009680433 >100 0.012116514645361398 1.6077634977887556 >500 0.0021056069588655892 1.0036887866797701 >1k 9.625631811906487E-4 1.3433264769752031 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 3211 0.13245120528121854 No Hit GTCCTACAGTGGACATTTCTAAATT 3090 0.12746005117376683 No Hit CTTTAGGACGTGAAATATGGCGAGG 2904 0.11978769857884106 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2891 0.11925145888134624 No Hit CCCATGTACTCTGCGTTGATACCAC 2720 0.1121978443989145 No Hit GTACATGGGAAGCAGTGGTATCAAC 2652 0.10939289828894162 No Hit GTATCAACGCAGAGTACTTTTTTTT 2637 0.1087741601764476 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 585 0.0 13.661825 4 TGAACCG 80 1.9667768E-6 13.082418 5 CTACACT 370 0.0 11.5716715 4 CCTACAC 350 0.0 11.408653 3 CGAATGC 60 0.005913728 11.074127 14 ACCGCTA 70 0.0014907067 10.858205 10 GGCGAGG 680 0.0 10.6064205 19 GAACCGT 100 2.3663442E-5 10.463338 6 GTAGGAC 1935 0.0 10.317904 3 TGTAGGA 2110 0.0 10.272984 2 GTCCTAC 1735 0.0 10.19238 1 ACCGTCC 85 6.569856E-4 10.063133 8 GTCTAGG 180 3.8926373E-10 10.035598 1 GGACGTG 1680 0.0 10.021703 6 AGGACGT 1710 0.0 10.015248 5 TGGCGAG 1705 0.0 9.907403 18 GTCCTAA 925 0.0 9.867147 1 TCTACAC 300 0.0 9.824119 3 TAGGACA 650 0.0 9.807239 4 CTGTAGG 2170 0.0 9.726473 1 >>END_MODULE