FastQCFastQC Report
Thu 26 May 2016
SRR1512957_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512957_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838153
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT34330.18676356103110023No Hit
GTATCAACGCAGAGTACTTTTTTTT32670.17773275674005373No Hit
GTCCTAAAGTGTGTATTTCTCATTT31660.1722381107557423No Hit
CTGTAGGACGTGGAATATGGCAAGA31540.17158528152988353No Hit
CTTTAGGACGTGAAATATGGCGAGG28120.15297964859290822No Hit
GTACATGGGAAGCAGTGGTATCAAC27000.14688657581822623No Hit
CCCATGTACTCTGCGTTGATACCAC22900.12458157726805114No Hit
TATCAACGCAGAGTACTTTTTTTTT19900.10826084662158156No Hit
GGTATCAACGCAGAGTACTTTTTTT19610.10668317599242282No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG500.001502938113.2962274
TAGGACC5450.011.8498494
GGCGAGG7750.011.63774119
GTCCTAA8650.011.4595831
TTAGGAC11100.010.9518473
CGCTCCT700.00149957910.850224510
TCAATAC1158.850202E-710.7360853
AGGACGT17050.010.6949145
GGACGTG16600.010.5231246
TGTAGGA16200.010.435312
GACGTGA10450.010.3567517
GTCCTAC15250.010.3125421
CGTGAAA11000.010.2707249
GGGGGCG1409.783071E-810.1718097
TGGCGAG17150.010.14049918
AGGACCT11450.010.11945
TAGGACG17950.010.1057674
GTAGGAC16100.010.08723
AAGGCCG856.6113804E-410.055975
AATCCCG856.6329166E-410.05241419
Produced by FastQC (version 0.11.5)