##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512957_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1838153
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.158997646006615	31.0	30.0	34.0	23.0	34.0
2	29.23846600364605	31.0	30.0	34.0	22.0	34.0
3	29.36875548444553	31.0	30.0	34.0	23.0	34.0
4	32.7947842209	35.0	33.0	37.0	25.0	37.0
5	32.92210169664875	37.0	35.0	37.0	26.0	37.0
6	32.88535393952517	37.0	35.0	37.0	26.0	37.0
7	32.7829614836197	37.0	35.0	37.0	25.0	37.0
8	32.75616229987384	37.0	35.0	37.0	25.0	37.0
9	34.07688587402681	39.0	35.0	39.0	25.0	39.0
10	33.95562882959144	39.0	34.0	39.0	24.0	39.0
11	34.02998879853853	39.0	35.0	39.0	25.0	39.0
12	33.92757240556145	39.0	35.0	39.0	23.0	39.0
13	33.93013040807811	39.0	35.0	39.0	23.0	39.0
14	34.93695084141527	39.0	35.0	41.0	23.0	41.0
15	34.88714541172579	39.0	35.0	41.0	22.0	41.0
16	34.77284535074066	39.0	34.0	41.0	19.0	41.0
17	34.725044106774575	39.0	34.0	41.0	19.0	41.0
18	34.41226492027595	39.0	34.0	41.0	17.0	41.0
19	34.65787233162854	39.0	34.0	41.0	18.0	41.0
20	34.742834791227935	39.0	34.0	41.0	19.0	41.0
21	34.6812060802338	39.0	34.0	41.0	18.0	41.0
22	34.596996550341565	39.0	34.0	41.0	18.0	41.0
23	34.328956294715404	39.0	34.0	41.0	16.0	41.0
24	34.340590799568915	39.0	34.0	41.0	16.0	41.0
25	34.257593899963716	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5399167143917118
1101	2	0.7882188885005483
1101	3	0.8833369380259164
1101	4	0.7906440146801188
1101	5	0.6192061471745589
1101	6	0.8753086617289725
1101	7	0.9437316428608185
1101	8	0.7337845201920175
1101	9	0.5567682702201395
1101	10	0.6941714844511395
1101	11	0.9103068759708677
1101	12	1.089559255715642
1101	13	0.9998892723692947
1101	14	0.9701548758092784
1101	15	0.7642493220288955
1101	16	1.2397079978271464
1101	17	1.1963618850163584
1101	18	1.4173983708803846
1101	19	0.8328196250212017
1101	20	1.0859653717322502
1101	21	1.0969316351586258
1101	22	1.1094666758781173
1101	23	1.3843303668905378
1101	24	0.9716323466988896
1101	25	1.1867784347731245
1102	1	0.790895323882328
1102	2	0.8945238394528197
1102	3	0.9238847428740478
1102	4	0.9873905214501164
1102	5	0.9421689257400345
1102	6	0.9125499390263201
1102	7	1.0899368027033702
1102	8	1.10306213501854
1102	9	1.0654666203837522
1102	10	1.2776879124633638
1102	11	1.1909702739916384
1102	12	1.2876893449359699
1102	13	1.184210595413255
1102	14	1.0722621783105524
1102	15	1.4434963068293314
1102	16	1.2915607716664326
1102	17	1.159907103088436
1102	18	1.5104411338602688
1102	19	1.4701009486424041
1102	20	1.4605861041831218
1102	21	1.3023638648730014
1102	22	1.5326011846251717
1102	23	1.719130261543448
1102	24	1.700058742637971
1102	25	1.5240460488420169
1103	1	0.4526857245809275
1103	2	0.6603189044709481
1103	3	0.6461514014522969
1103	4	0.734233605443066
1103	5	0.33991300677996605
1103	6	0.6296988025665158
1103	7	0.5911957495767126
1103	8	0.6260025286397735
1103	9	0.8551052854057701
1103	10	0.7665431245858016
1103	11	0.721279738261444
1103	12	1.016737540657644
1103	13	0.951859630083348
1103	14	1.0977601588279597
1103	15	1.0064885861372943
1103	16	1.139635990933641
1103	17	1.0287609371845505
1103	18	1.251554549171189
1103	19	1.3060907272819477
1103	20	1.1973123030847432
1103	21	1.0497308235464402
1103	22	1.1633350660235422
1103	23	1.426494389271511
1103	24	1.3651949762673539
1103	25	1.2903230486090962
1104	1	0.6353500753174899
1104	2	0.6482266633931602
1104	3	0.6121071547248853
1104	4	0.9004928330929829
1104	5	0.5654576713317354
1104	6	0.6262712705194033
1104	7	0.8426729710095273
1104	8	0.72788379530963
1104	9	0.9412757629346871
1104	10	0.9698557502993737
1104	11	0.9239106407954267
1104	12	0.9617286370858693
1104	13	0.9801682036610444
1104	14	1.1711177270865107
1104	15	1.2284488258753754
1104	16	0.9913537812752011
1104	17	1.0012127186221704
1104	18	1.2826259932411403
1104	19	1.1364017421555985
1104	20	1.1046658262546103
1104	21	1.058716736207984
1104	22	1.1129120304563571
1104	23	1.3239362211855763
1104	24	1.1970919804315088
1104	25	1.0095766099450856
1105	1	0.6399098296132522
1105	2	0.8523845211941001
1105	3	0.8170377778181255
1105	4	0.9172807199970308
1105	5	0.7136065106104539
1105	6	0.5083524825900554
1105	7	0.7925456769540418
1105	8	0.8349802102201949
1105	9	0.7190113410926529
1105	10	1.009102860296224
1105	11	0.8722209850931009
1105	12	0.9137652322058827
1105	13	0.9230099163021279
1105	14	1.051101276552636
1105	15	1.194870856711347
1105	16	1.135392548334032
1105	17	1.04338989701899
1105	18	1.0610553038774242
1105	19	1.034975565755822
1105	20	1.0425500592814032
1105	21	1.0921984756031335
1105	22	0.7791136475555476
1105	23	0.9728874630618449
1105	24	1.0627951053743345
1105	25	1.1142676465263008
1106	1	0.6842590014235626
1106	2	0.6999846921103341
1106	3	0.8471039539982925
1106	4	0.8113528710639955
1106	5	0.8376058786512743
1106	6	0.7830324634876504
1106	7	0.6158678432813858
1106	8	0.7142383045041996
1106	9	0.9188418611304243
1106	10	0.9928335020579553
1106	11	0.9308150913555977
1106	12	1.0682893059009473
1106	13	0.9465410600405235
1106	14	1.1940396713045018
1106	15	1.1996794915717928
1106	16	1.162488516362643
1106	17	1.2201653971105557
1106	18	1.35546066978538
1106	19	0.9488165490268585
1106	20	0.9737092375908389
1106	21	0.9567319209655949
1106	22	0.94952323609985
1106	23	1.254453644628029
1106	24	1.2528097912439407
1106	25	1.216335158438369
1107	1	0.429011825682462
1107	2	0.5803256558975285
1107	3	0.6820858334399524
1107	4	0.8182078924405971
1107	5	0.6879569644691088
1107	6	0.5842609099329081
1107	7	0.7718543519594334
1107	8	0.7574905200255913
1107	9	0.9331655962305092
1107	10	0.7439164885524718
1107	11	0.7526648747583025
1107	12	0.9278659502000437
1107	13	0.8319030465067314
1107	14	1.0883614244685234
1107	15	1.1002549608659393
1107	16	0.9567950510889958
1107	17	1.152077399149121
1107	18	1.5060973667473547
1107	19	1.323224091709271
1107	20	1.2375502296914078
1107	21	1.1729749487976378
1107	22	1.1581779262099872
1107	23	1.2538388905296642
1107	24	1.3366219744880894
1107	25	1.4843491877144004
1108	1	0.47488599337057025
1108	2	0.465658193532299
1108	3	0.25665645471915255
1108	4	0.39497776205050883
1108	5	0.4105095617299668
1108	6	0.562240325313681
1108	7	0.552466009083318
1108	8	0.570553362488468
1108	9	0.6412412370337108
1108	10	0.6067550335714742
1108	11	0.6581580632800907
1108	12	0.6745834604191288
1108	13	0.6544417446953759
1108	14	0.784141983304508
1108	15	0.8499902721585144
1108	16	0.7472449659221994
1108	17	0.9359925345857789
1108	18	1.1083620060151276
1108	19	0.9674703422246509
1108	20	0.8756501052527383
1108	21	0.7424942134714243
1108	22	0.524928966774759
1108	23	0.7640473626205164
1108	24	0.8863973272353505
1108	25	1.0221571127974656
1109	1	0.3240244886757182
1109	2	0.32336457463761903
1109	3	0.47034718164945133
1109	4	0.6322708024192636
1109	5	0.5326841397089623
1109	6	0.3545837307002202
1109	7	0.5964112897966842
1109	8	0.5689388356193277
1109	9	0.6487216841641583
1109	10	0.8268248700850407
1109	11	0.3816712575913854
1109	12	0.6135577660276468
1109	13	0.6239504585787827
1109	14	0.5250949426131086
1109	15	0.7896651049502665
1109	16	0.6922504340019415
1109	17	0.5728424264715244
1109	18	-0.776710195683485
1109	19	0.5278683300473332
1109	20	0.7320857488170915
1109	21	0.6654648526273377
1109	22	0.8998115093807542
1109	23	0.5093596989077795
1109	24	0.8078229339094705
1109	25	0.8525021874748262
1110	1	0.39336497331229836
1110	2	0.31688936374772325
1110	3	0.29218623772268515
1110	4	0.18122102052269895
1110	5	0.34905363691124336
1110	6	0.35527750329556085
1110	7	0.428655520380957
1110	8	0.2957918661538059
1110	9	0.5794681863055899
1110	10	0.6531639006987291
1110	11	0.5668753016986372
1110	12	0.03974064476666683
1110	13	0.3211240247820868
1110	14	0.4574637835249007
1110	15	0.5837893248981061
1110	16	0.6842568441495516
1110	17	0.3357150551978094
1110	18	0.82140735386362
1110	19	0.5816707130607242
1110	20	0.6148770767303731
1110	21	0.516107140365456
1110	22	0.6135069057292952
1110	23	0.6476785768773468
1110	24	0.7986843717514063
1110	25	0.7193579619824675
1111	1	-0.16362007856781702
1111	2	0.16788370878630943
1111	3	0.07794155034980577
1111	4	0.07039065217672658
1111	5	-0.021024770532456216
1111	6	0.13776560037526764
1111	7	0.07522329684096007
1111	8	-0.15107040373739267
1111	9	0.22286972867196653
1111	10	0.18089011347882433
1111	11	0.03030425945544124
1111	12	0.17444703939931827
1111	13	0.27889873730722314
1111	14	0.1321552529554495
1111	15	0.39457299827395076
1111	16	0.1768436595525884
1111	17	0.2645267111790659
1111	18	0.34648441488792514
1111	19	0.1189414636752133
1111	20	0.04850576291152464
1111	21	0.4287982889471351
1111	22	0.07246904556210865
1111	23	0.40322038176685737
1111	24	0.44575490693878805
1111	25	3.649977399291515E-4
1112	1	-0.046783243335745794
1112	2	0.28534057500323584
1112	3	0.3215073273113731
1112	4	0.33897046870099246
1112	5	0.23611894544516332
1112	6	0.2569231979854152
1112	7	-0.13515304932288785
1112	8	0.16540782026866196
1112	9	0.44460511881912623
1112	10	0.5037697578067224
1112	11	0.388388733488334
1112	12	0.470020748177042
1112	13	0.1990702847795518
1112	14	0.46089787592269715
1112	15	0.5279268959285233
1112	16	0.5556179401234047
1112	17	0.5391028642959412
1112	18	0.631981367694479
1112	19	0.43931378762959383
1112	20	0.42267478063835284
1112	21	0.5234049020702258
1112	22	0.3838240816498697
1112	23	0.5870873616219328
1112	24	0.32429706355875965
1112	25	0.6492864682749797
1113	1	-0.3951826665979361
1113	2	-0.41505087516353356
1113	3	-0.5816694875330661
1113	4	-0.19665029570752068
1113	5	-0.4595784279223665
1113	6	-0.38367673310901296
1113	7	-0.4482526825346369
1113	8	-0.1678695542257458
1113	9	-0.29916243663092246
1113	10	-0.6087382203688705
1113	11	-0.5502825342726325
1113	12	-0.2510077367962751
1113	13	-0.32503304217739526
1113	14	-0.31451544206544924
1113	15	-0.7657413191122941
1113	16	-0.7748177721764264
1113	17	-0.6155386558386198
1113	18	-0.742493181117176
1113	19	-0.7221411637347117
1113	20	-0.8576141147572613
1113	21	-0.7228341559370008
1113	22	-0.5734540003736299
1113	23	-1.1709215259040349
1113	24	-0.4562947946841831
1113	25	-0.4402900857258416
1114	1	-1.208992172076087
1114	2	-0.9950556448971319
1114	3	-0.7691122718556684
1114	4	-0.9828577847526248
1114	5	-0.9544533116131646
1114	6	-1.4591105863871228
1114	7	-1.6435117739003857
1114	8	-1.0574246357290384
1114	9	-1.2027525531419982
1114	10	-1.3237332581558618
1114	11	-2.080497576021884
1114	12	-1.5181020207212441
1114	13	-1.4278177497354534
1114	14	-1.5891016348133888
1114	15	-1.7643162673071657
1114	16	-2.2259343405221017
1114	17	-4.3110759034206545
1114	18	-3.251896034882588
1114	19	-2.2183932141098595
1114	20	-1.3357050288842132
1114	21	-2.4452915264429578
1114	22	-2.6097861315713473
1114	23	-2.3335023085722497
1114	24	-1.8514111226653398
1114	25	-1.7401061035804162
1115	1	-1.04453406669505
1115	2	-0.6242450581897181
1115	3	-0.6894062476936966
1115	4	-0.6434654890542646
1115	5	-0.5794263364478169
1115	6	-0.5923506488334169
1115	7	-1.6329861129845327
1115	8	-1.0865286519666313
1115	9	-0.8699915307492034
1115	10	-0.6746546423172148
1115	11	-1.3039017641228696
1115	12	-1.0364759966370372
1115	13	-0.7276736877713077
1115	14	-0.7751133833484616
1115	15	-1.9663318224998108
1115	16	-0.7917462103725228
1115	17	-0.7897988164663303
1115	18	-0.9575962518504042
1115	19	-1.082050521609247
1115	20	-0.9070385449682163
1115	21	-1.7849010815811681
1115	22	-1.885439513819648
1115	23	-1.219840140682706
1115	24	-1.0648726536493527
1115	25	-1.5481308993468232
1116	1	-1.0489365540015427
1116	2	-1.733520662178595
1116	3	-1.3329203211569656
1116	4	-0.9617977022336746
1116	5	-1.4919734958692494
1116	6	-1.3069004834459825
1116	7	-0.926232055485805
1116	8	-1.6554858922085849
1116	9	-1.5814597654430997
1116	10	-2.540805543994054
1116	11	-1.2530541888325288
1116	12	-1.3167532159067008
1116	13	-1.8302906120733766
1116	14	-1.6744803787191245
1116	15	-1.1842651808718756
1116	16	-1.1179702138591168
1116	17	-1.4469287609104597
1116	18	-1.4601077734336059
1116	19	-1.7334901547258923
1116	20	-2.0804767303565157
1116	21	-2.46917746214735
1116	22	-1.7559816071331795
1116	23	-1.5124907072546776
1116	24	-2.1117896888372485
1116	25	-1.855711752809981
1201	1	0.22642300075291288
1201	2	0.2981807879936156
1201	3	0.49008875400876306
1201	4	0.5405626922504894
1201	5	0.5497471094720936
1201	6	0.6305373079506325
1201	7	0.5276781111120954
1201	8	0.12075746514216945
1201	9	0.45005204506730934
1201	10	0.6320819729902425
1201	11	0.2135962275870682
1201	12	-0.3454242796000102
1201	13	-0.015043174403437831
1201	14	0.6337322933229501
1201	15	0.25433936944582314
1201	16	0.8486158068478034
1201	17	0.7577335608393767
1201	18	1.060698174685939
1201	19	0.5355766582010162
1201	20	0.6337457993403675
1201	21	0.5049336575552061
1201	22	0.7696661049069959
1201	23	1.0351826233360768
1201	24	0.8675330943418444
1201	25	0.845739381426398
1202	1	0.646157332162268
1202	2	0.63831394643314
1202	3	0.40077405968755286
1202	4	0.6414039545112473
1202	5	0.6986178991089886
1202	6	0.5452852210021888
1202	7	0.48456044892654404
1202	8	0.6077776506823085
1202	9	0.9356784105247797
1202	10	0.9983670859112763
1202	11	0.6378815436384784
1202	12	0.8108996657968177
1202	13	0.8705749794008284
1202	14	1.014824313540906
1202	15	0.5593072035358588
1202	16	0.8446871790798767
1202	17	1.021122337238225
1202	18	1.3092152149468106
1202	19	1.1147856166824681
1202	20	1.020391490275351
1202	21	1.0166341828849141
1202	22	1.0591895480963984
1202	23	1.3430763991190489
1202	24	1.3204230560279981
1202	25	1.0855088500676118
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2211	14	0.1648839555527033
2211	15	0.1198402599416184
2211	16	0.03966806130519984
2211	17	0.09941247478069926
2211	18	0.1389497295979325
2211	19	0.08239661317686853
2211	20	-0.027403554150474463
2211	21	-0.002200270969524354
2211	22	-0.08282067090051015
2211	23	0.5433425335169133
2211	24	0.2580297571307284
2211	25	0.22169136264566447
2212	1	-0.09839604806190394
2212	2	-0.08535116425267475
2212	3	-0.11165294436191431
2212	4	-0.2185713853362543
2212	5	-0.16301363406665814
2212	6	-0.2280361871658556
2212	7	-0.18248867346179765
2212	8	-0.2331224413005586
2212	9	-0.13668025315438825
2212	10	-0.22205975235164033
2212	11	-0.42435088218070405
2212	12	-0.24783858148337856
2212	13	-0.3052509762517488
2212	14	-0.27692633631729535
2212	15	-0.18612372265556587
2212	16	-0.11245424986881858
2212	17	-0.25421759387435827
2212	18	-3.028763366816122
2212	19	-1.1559408377750273
2212	20	-0.5559780016067819
2212	21	-0.6854311377335023
2212	22	-0.4235481659913134
2212	23	-0.41370505323565254
2212	24	-0.849158909835019
2212	25	-0.407130071717809
2213	1	-0.17342811081966047
2213	2	-0.11028055040952012
2213	3	-0.36041080849917506
2213	4	-0.3368105005746358
2213	5	-0.25017839591188107
2213	6	-0.1703163559949843
2213	7	0.06188968791816052
2213	8	-0.4329802190530785
2213	9	-0.31195567555872117
2213	10	-0.49604939660184755
2213	11	-0.462115553852378
2213	12	-0.37195164603892295
2213	13	-0.2696382271141786
2213	14	-0.4075325829192096
2213	15	-0.5364009447420202
2213	16	-0.9526250728539765
2213	17	-0.6086054320014753
2213	18	-0.44261779337109175
2213	19	-0.3282511654876643
2213	20	-1.0633053126354213
2213	21	-0.6778641848226599
2213	22	-0.6357058238281681
2213	23	-1.1699178637518628
2213	24	-0.6643167561649008
2213	25	-0.835944947890205
2214	1	-1.9073367301333093
2214	2	-3.8796001832906057
2214	3	-2.0273501540698042
2214	4	-1.698201162984855
2214	5	-1.8433428013388564
2214	6	-1.845102868365295
2214	7	-2.059816217907855
2214	8	-1.8460867633467757
2214	9	-2.0072110626297572
2214	10	-2.0873422936489305
2214	11	-1.7624288020566894
2214	12	-2.2954751895345353
2214	13	-2.127607101814892
2214	14	-3.5850246020732435
2214	15	-3.63680397746349
2214	16	-2.8543329738914878
2214	17	-3.2679909068618116
2214	18	-2.6275599322378156
2214	19	-4.711293863013605
2214	20	-3.135057108325764
2214	21	-3.0433131074084514
2214	22	-3.0473099738680745
2214	23	-3.0362606410862583
2214	24	-3.200298400219168
2214	25	-3.0440953868367338
2215	1	-1.0859081587047363
2215	2	-2.57138589757632
2215	3	-1.3887787254983763
2215	4	-1.0632011629848535
2215	5	-1.0279856584817146
2215	6	-1.473674296936725
2215	7	-2.1187447893364286
2215	8	-1.8760867633467768
2215	9	-1.5386396340583275
2215	10	-3.0616280079346474
2215	11	-2.103143087770974
2215	12	-2.5111894752488233
2215	13	-1.7522499589577478
2215	14	-3.1175246020732423
2215	15	-2.870375406034917
2215	16	-2.6407615453200606
2215	17	-2.8419194782903823
2215	18	-1.8950599322378139
2215	19	-2.450936720156463
2215	20	-2.557557108325767
2215	21	-2.31117025026559
2215	22	-1.9815956881537886
2215	23	-2.339832069657689
2215	24	-2.297441257362024
2215	25	-2.330881101122447
2216	1	-2.0566074758406643
2216	2	-2.0638375280379933
2216	3	-2.5273396389527782
2216	4	-3.2852617548003806
2216	5	-2.389500206455388
2216	6	-2.445032478934845
2216	7	-2.3153896010539796
2216	8	-2.7970310208558047
2216	9	-3.1479205237609342
2216	10	-2.5884070420283685
2216	11	-3.1242909674285535
2216	12	-3.5070350143650586
2216	13	-4.342274205580541
2216	14	-3.5651167639813046
2216	15	-3.2849074214735836
2216	16	-2.313152930346533
2216	17	-2.5557801344336823
2216	18	-2.687653331218467
2216	19	-2.974970566462563
2216	20	-2.927941961608955
2216	21	-3.3413994798167828
2216	22	-3.6117443842792767
2216	23	-3.639623251804256
2216	24	-3.619378141918414
2216	25	-3.765327263723268
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	88319.0
3	4.0
4	660.0
5	4228.0
6	5516.0
7	4852.0
8	4274.0
9	4081.0
10	3704.0
11	3548.0
12	3351.0
13	3308.0
14	3258.0
15	3366.0
16	3455.0
17	3776.0
18	4134.0
19	5135.0
20	6424.0
21	8187.0
22	10331.0
23	12518.0
24	14649.0
25	16457.0
26	18452.0
27	20400.0
28	23382.0
29	27544.0
30	33736.0
31	42426.0
32	53419.0
33	68379.0
34	89611.0
35	118178.0
36	161693.0
37	249044.0
38	714354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24588755130153	18.58244252344842	12.537474618811261	25.634195306438784
2	14.809630352471077	19.906890120218854	41.29875982298891	23.984719704321165
3	18.551395689420126	24.308305020084582	29.63620264503703	27.504096645458258
4	12.349692159747132	16.618693329284305	38.07423105243652	32.95738345853204
5	13.067127324104947	37.84030904976341	36.1009244715556	12.991639154576044
6	32.219382494301655	37.13506216356071	17.832445412019613	12.813109930118028
7	28.209026366078	31.51566859794452	22.138342467363437	18.136962568614052
8	25.125551302038694	36.94987470717393	20.47385447530738	17.45071951547999
9	26.39195800523792	15.031073272632394	20.188295162625227	38.38867355950446
10	14.69698404251267	28.914144299739135	34.93686328667866	21.45200837106953
11	34.0529346052323	22.433570558343042	24.839792858437125	18.673701977987538
12	23.240998149378512	24.733466430074422	31.739165212451454	20.28637020809561
13	28.824017856462685	22.005086610591338	25.720195062918783	23.450700470027197
14	20.67319282271577	22.38860794142023	27.66551064930881	29.27268858655519
15	22.86966231968289	31.324676370649478	23.218215538126987	22.587445771540644
16	22.000888045342553	28.258941397713716	27.908457325938755	21.831713231004972
17	21.65516956166618	28.925961618515228	27.869700806819797	21.54916801299879
18	21.52707808907193	27.55621321755384	29.89814468878633	21.0185640045879
19	23.326081372873386	27.286598638271197	27.646457676702596	21.74086231215282
20	23.397771806617104	27.468518089243094	28.139022115423334	20.994687988716464
21	22.575092862763366	27.068856120126817	27.416907825068648	22.939143192041175
22	23.039465350284367	28.266142986803526	27.073498011714236	21.62089365119787
23	21.851026758069484	28.117412497837	27.950412629521487	22.08114811457203
24	22.90528753494679	27.10934957889731	28.324882984708843	21.66047990144706
25	22.427850972180806	27.51517638121938	27.888464208988946	22.16850843761087
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	215.0
1	215.0
2	395.0
3	575.0
4	575.0
5	575.0
6	1160.5
7	1746.0
8	1746.0
9	1746.0
10	2584.5
11	3423.0
12	3423.0
13	3423.0
14	5833.0
15	8243.0
16	8243.0
17	8243.0
18	14902.5
19	21562.0
20	21562.0
21	21562.0
22	33751.5
23	45941.0
24	45941.0
25	45941.0
26	65981.5
27	86022.0
28	86022.0
29	86022.0
30	111229.0
31	136436.0
32	136436.0
33	136436.0
34	156201.0
35	175966.0
36	175966.0
37	175966.0
38	190882.0
39	205798.0
40	205798.0
41	205798.0
42	216767.5
43	227737.0
44	227737.0
45	227737.0
46	236760.0
47	245783.0
48	245783.0
49	245783.0
50	234978.5
51	224174.0
52	224174.0
53	224174.0
54	199779.5
55	175385.0
56	175385.0
57	175385.0
58	150061.5
59	124738.0
60	124738.0
61	124738.0
62	101693.0
63	78648.0
64	78648.0
65	78648.0
66	60876.0
67	43104.0
68	43104.0
69	43104.0
70	31681.0
71	20258.0
72	20258.0
73	20258.0
74	14103.5
75	7949.0
76	7949.0
77	7949.0
78	5472.5
79	2996.0
80	2996.0
81	2996.0
82	2015.0
83	1034.0
84	1034.0
85	1034.0
86	678.0
87	322.0
88	322.0
89	322.0
90	201.0
91	80.0
92	80.0
93	80.0
94	45.5
95	11.0
96	11.0
97	11.0
98	9.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3579680255125661
2	0.041019436358126875
3	0.036667241519068326
4	0.036667241519068326
5	0.04237949724533268
6	0.01849682806599886
7	0.018333620759534163
8	0.024916315453610227
9	0.021760974195292774
10	0.021488962017851616
11	0.020455315743575207
12	0.02132575471138692
13	0.02105374253394576
14	0.02839807132485707
15	0.030302156566945185
16	0.02225059611468686
17	0.02415468135677498
18	0.10983851725074029
19	0.026602790953745414
20	0.023447449695427964
21	0.02692920556667481
22	0.021162547404922225
23	0.023229839953475037
24	0.019530474340275265
25	0.023338644824451502
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1838153.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.87237335936712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.46528335017449	59.26975634129741
2	11.944750624790531	17.16995153178957
3	3.1197738528548156	6.726766534475176
4	1.1270147380559405	3.2400489614026355
5	0.46705984554794117	1.678434980019498
6	0.254711636729578	1.0984037912402227
7	0.14652163002746635	0.7371600108989982
8	0.09691457993263985	0.5572384698307935
9	0.06644537452501416	0.4298028089278318
>10	0.2792996645309615	3.476056617823333
>50	0.015702441022496384	0.7718808921749248
>100	0.013112482955679523	1.9294861991009566
>500	0.0021974130382057004	1.0787932923050525
>1k	0.001212365814182453	1.836219568713686
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3433	0.18676356103110023	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3267	0.17773275674005373	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3166	0.1722381107557423	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3154	0.17158528152988353	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2812	0.15297964859290822	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2700	0.14688657581822623	No Hit
CCCATGTACTCTGCGTTGATACCAC	2290	0.12458157726805114	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1990	0.10826084662158156	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1961	0.10668317599242282	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.0880487097646387E-4	0.0	0.0	0.0	0.0
7	1.0880487097646387E-4	0.0	0.0	0.0	0.0
8	1.0880487097646387E-4	0.0	0.0	5.4402435488231934E-5	0.0
9	1.0880487097646387E-4	0.0	0.0	5.4402435488231934E-5	5.4402435488231934E-5
10	1.0880487097646387E-4	0.0	0.0	5.4402435488231934E-5	5.4402435488231934E-5
11	1.0880487097646387E-4	0.0	0.0	5.4402435488231934E-5	5.4402435488231934E-5
12	1.0880487097646387E-4	0.0	0.0	5.4402435488231934E-5	5.4402435488231934E-5
13	1.0880487097646387E-4	0.0	0.0	5.4402435488231934E-5	5.4402435488231934E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	50	0.0015029381	13.296227	4
TAGGACC	545	0.0	11.849849	4
GGCGAGG	775	0.0	11.637741	19
GTCCTAA	865	0.0	11.459583	1
TTAGGAC	1110	0.0	10.951847	3
CGCTCCT	70	0.001499579	10.8502245	10
TCAATAC	115	8.850202E-7	10.736085	3
AGGACGT	1705	0.0	10.694914	5
GGACGTG	1660	0.0	10.523124	6
TGTAGGA	1620	0.0	10.43531	2
GACGTGA	1045	0.0	10.356751	7
GTCCTAC	1525	0.0	10.312542	1
CGTGAAA	1100	0.0	10.270724	9
GGGGGCG	140	9.783071E-8	10.171809	7
TGGCGAG	1715	0.0	10.140499	18
AGGACCT	1145	0.0	10.1194	5
TAGGACG	1795	0.0	10.105767	4
GTAGGAC	1610	0.0	10.0872	3
AAGGCCG	85	6.6113804E-4	10.05597	5
AATCCCG	85	6.6329166E-4	10.052414	19
>>END_MODULE