FastQCFastQC Report
Thu 26 May 2016
SRR1512957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512957_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838153
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42800.23284242388963267No Hit
GTCCTACAGTGGACATTTCTAAATT33260.18094250043385943No Hit
TATCAACGCAGAGTACTTTTTTTTT30760.16734189156180143No Hit
CTGTAGGACGTGGAATATGGCAAGA30220.1644041600454369No Hit
GTCCTAAAGTGTGTATTTCTCATTT29040.15798467265782554No Hit
CTTTAGGACGTGAAATATGGCGAGG27720.15080355117337893No Hit
GTACATGGGAAGCAGTGGTATCAAC26760.14558091736650866No Hit
CCCATGTACTCTGCGTTGATACCAC26460.1439488443018617No Hit
GGTATCAACGCAGAGTACTTTTTTT25830.14052149086610308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC350.002156803416.3010432
TAGGACC5100.012.31576254
CCCCTAA1251.8111677E-811.4088651
GGCGAGG6900.010.58891119
ACCGTGC909.475587E-510.5600218
GTTGGAC1002.374241E-510.4598363
GTGTACG1002.378531E-510.4581261
AATGTCC4750.010.4044018
GTCCTAC14750.010.2486411
AGGACCT12450.010.2429025
CTATTAG1303.8007602E-710.2387251
TGGCGAG16650.010.14386818
CTAGACT1803.8198777E-1010.0454334
TGTAGGA16450.010.0002862
TGTCCAC6250.09.72919910
GTAGGAC16200.09.6263363
GGACCTG12400.09.513876
TCCTACA17450.09.4816952
GTCCTAA9550.09.457611
AGGACGT16700.09.3457675
Produced by FastQC (version 0.11.5)