##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512957_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1838153 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.316616734297963 31.0 31.0 34.0 26.0 34.0 2 30.46868514209644 31.0 31.0 34.0 27.0 34.0 3 30.590878996470913 31.0 31.0 34.0 27.0 34.0 4 34.059622893197684 37.0 35.0 37.0 30.0 37.0 5 33.68636669526421 37.0 35.0 37.0 28.0 37.0 6 33.540029040020066 37.0 35.0 37.0 28.0 37.0 7 33.40673382465986 37.0 35.0 37.0 27.0 37.0 8 33.29361647262225 37.0 35.0 37.0 27.0 37.0 9 34.732762724321645 39.0 35.0 39.0 27.0 39.0 10 34.62150321545595 39.0 35.0 39.0 27.0 39.0 11 34.64715886000784 39.0 35.0 39.0 27.0 39.0 12 34.49541414670052 39.0 35.0 39.0 27.0 39.0 13 34.47500344095405 39.0 35.0 39.0 27.0 39.0 14 35.53641345415752 40.0 36.0 41.0 26.0 41.0 15 35.548569678367365 40.0 36.0 41.0 27.0 41.0 16 35.51912816832984 40.0 36.0 41.0 26.0 41.0 17 35.498604849541906 40.0 36.0 41.0 26.0 41.0 18 35.42786645072527 40.0 36.0 41.0 26.0 41.0 19 35.39982199523108 40.0 36.0 41.0 25.0 41.0 20 35.38138827399025 40.0 36.0 41.0 25.0 41.0 21 35.32505455204218 40.0 36.0 41.0 25.0 41.0 22 35.285544783268854 40.0 36.0 41.0 25.0 41.0 23 35.181807499158126 40.0 36.0 41.0 25.0 41.0 24 35.16714005852614 40.0 36.0 41.0 25.0 41.0 25 35.07532234803087 40.0 36.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6270929483439218 1101 2 0.6521407379011173 1101 3 0.6662355673133966 1101 4 0.4733738427410614 1101 5 0.7572431511179971 1101 6 0.6219501827012266 1101 7 0.6272535512015409 1101 8 0.4846964402114864 1101 9 0.39593441623648573 1101 10 0.8265551470238961 1101 11 0.7803664610390726 1101 12 0.8610595203512261 1101 13 0.8121436879742276 1101 14 0.8510150263109679 1101 15 0.8202367622575579 1101 16 1.0793080986103405 1101 17 0.9540903660625091 1101 18 0.5374646804849021 1101 19 0.7852179418222747 1101 20 0.7870258300091066 1101 21 0.980306212104189 1101 22 0.9500288372635808 1101 23 0.8976656304055339 1101 24 0.9102540641820056 1101 25 0.9291672561295101 1102 1 0.7356419365428835 1102 2 0.7239661647913245 1102 3 0.7007114835985391 1102 4 0.6192218326681456 1102 5 0.8620057774352858 1102 6 0.7635121336426778 1102 7 0.8819445631468952 1102 8 0.8856719830917186 1102 9 1.0733360231403282 1102 10 1.1201488175400627 1102 11 1.0013103276379098 1102 12 0.9805508959604907 1102 13 1.1578354611605093 1102 14 1.265267188271288 1102 15 1.2933552163973019 1102 16 1.2356312688360802 1102 17 1.2342035781023881 1102 18 1.2578331066643713 1102 19 1.2447909975010987 1102 20 1.280697789284524 1102 21 1.223113206862088 1102 22 1.3699019156713987 1102 23 1.371550737141618 1102 24 1.3536805142377446 1102 25 1.4319470482494054 1103 1 0.6130095767402111 1103 2 0.5225518903858415 1103 3 0.5314216988926859 1103 4 0.5425635015628743 1103 5 0.6153229638949824 1103 6 0.669604507839729 1103 7 0.7304972235989595 1103 8 0.7339482893520781 1103 9 0.7359509928499648 1103 10 0.9284001994982489 1103 11 0.9101302952393127 1103 12 0.9193736420823555 1103 13 0.9348048966864795 1103 14 1.0928723507772915 1103 15 0.9117718641535504 1103 16 0.9002443767123225 1103 17 0.9528499218765134 1103 18 1.0765496249310829 1103 19 1.1336187239898337 1103 20 1.0404201768948695 1103 21 0.7720923065465684 1103 22 0.9984979839526034 1103 23 0.9784061934143651 1103 24 1.0755996705261524 1103 25 1.1094020133756004 1104 1 0.5136263544743436 1104 2 0.5415451877636528 1104 3 0.5461336411769189 1104 4 0.529393781792642 1104 5 0.5966141505643634 1104 6 0.6524694439383865 1104 7 0.7107009171439884 1104 8 0.6442519899813917 1104 9 0.7644557751121042 1104 10 0.8327924180621125 1104 11 0.775803752803526 1104 12 0.822074534368241 1104 13 0.8251513181405059 1104 14 0.9108377965363417 1104 15 0.9951468315602838 1104 16 0.8832387586160237 1104 17 0.9901113975809466 1104 18 0.918547110937773 1104 19 1.0186040862285282 1104 20 1.0068302886522176 1104 21 0.9349781542626658 1104 22 0.9526838124929142 1104 23 0.7950078219093584 1104 24 0.9452050907817835 1104 25 1.0580847261294224 1105 1 0.5573132059753441 1105 2 0.6903482431137 1105 3 0.630459092450625 1105 4 0.58299961734145 1105 5 0.45280955480622964 1105 6 0.6167370988079384 1105 7 0.6231058490842969 1105 8 0.7917711279968316 1105 9 0.8810017337911162 1105 10 0.8854483962724728 1105 11 0.8366864615215093 1105 12 0.7406743948899575 1105 13 0.9219559043854275 1105 14 1.0655130631440315 1105 15 0.8748197381603262 1105 16 0.9129035058098083 1105 17 0.657081023367887 1105 18 0.991225101250393 1105 19 1.0774571883762434 1105 20 1.0079488225057034 1105 21 0.9010141683451423 1105 22 0.9583134369187363 1105 23 1.0174658009671234 1105 24 0.9560333180830867 1105 25 1.065448681751036 1106 1 0.43055081185544 1106 2 0.4207627149789488 1106 3 0.3945052829216884 1106 4 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-2.735520540118493 2216 21 -2.5185408613588294 2216 22 -2.7189525157195717 2216 23 -1.980525169140229 2216 24 -2.204735161355295 2216 25 -2.119572901607917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51947.0 3 1.0 4 869.0 5 7504.0 6 8549.0 7 6698.0 8 5365.0 9 4684.0 10 4053.0 11 3667.0 12 3308.0 13 3078.0 14 2981.0 15 3058.0 16 2998.0 17 3230.0 18 3562.0 19 4286.0 20 5404.0 21 7128.0 22 8970.0 23 10781.0 24 12985.0 25 14506.0 26 16657.0 27 18307.0 28 20837.0 29 25053.0 30 31028.0 31 40112.0 32 51186.0 33 65630.0 34 85717.0 35 112173.0 36 154685.0 37 242931.0 38 794225.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.49474970232797 19.46881262750081 13.530452924263633 27.505984745907586 2 13.696970488278712 20.27918272220291 39.8391644647257 26.184682324792686 3 16.765198544408435 24.16050241737222 30.303244615654958 28.77105442256439 4 11.488734864591455 17.035133248188867 37.40330779180708 34.07282409541259 5 12.663699590325697 38.09758686864563 36.08433029820478 13.154383242823894 6 32.312276474242374 37.36052183277374 17.517232818494772 12.809968874489114 7 27.912744204879036 31.67379049945197 22.164426115132894 18.2490391805361 8 25.01533830002096 37.32816884923281 20.126462718540154 17.530030132206075 9 25.91652374402705 14.859346160901046 20.04151877734059 39.182611317731315 10 14.356522998866094 29.179787531846806 34.915371100366315 21.54831836892079 11 33.96513425157461 22.376948977709883 24.743441386360434 18.91447538435508 12 22.954483872336766 24.876462132283727 31.78331985779407 20.385734137585438 13 28.6393885714037 21.922999081638427 25.791814830451305 23.64579751650657 14 20.488775699515944 22.252085259091164 27.6362457229814 29.622893318411485 15 22.737340351736236 31.31671223187933 23.224656900749448 22.72129051563499 16 21.867567592567905 28.34896695147983 27.749994150656242 22.033471305296022 17 21.44188845910218 28.948946563024457 27.98464739954854 21.624517578324816 18 21.483184088263137 27.559707095215817 29.81709771834572 21.14001109817533 19 23.15208893584587 27.24808211136675 27.845060821320406 21.754768131466975 20 23.31429329993575 27.383372768091522 28.188734081696055 21.113599850276678 21 22.368891347881647 26.922325750169602 27.63864339763164 23.070139504317112 22 22.921536828624884 28.13939457573896 27.225866891095336 21.71320170454082 23 21.55734587926032 27.985320046807853 28.098749124800822 22.358584949131004 24 22.840100905637343 27.07141353304105 28.395242398211685 21.693243163109926 25 22.29700395519262 27.396264600754044 28.06043229656872 22.246299147484617 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 158.0 1 158.0 2 306.0 3 454.0 4 454.0 5 454.0 6 1128.0 7 1802.0 8 1802.0 9 1802.0 10 2553.5 11 3305.0 12 3305.0 13 3305.0 14 5705.0 15 8105.0 16 8105.0 17 8105.0 18 14859.0 19 21613.0 20 21613.0 21 21613.0 22 34416.0 23 47219.0 24 47219.0 25 47219.0 26 66997.5 27 86776.0 28 86776.0 29 86776.0 30 111805.5 31 136835.0 32 136835.0 33 136835.0 34 156231.5 35 175628.0 36 175628.0 37 175628.0 38 190624.0 39 205620.0 40 205620.0 41 205620.0 42 217223.0 43 228826.0 44 228826.0 45 228826.0 46 236946.0 47 245066.0 48 245066.0 49 245066.0 50 235144.5 51 225223.0 52 225223.0 53 225223.0 54 199846.0 55 174469.0 56 174469.0 57 174469.0 58 149549.0 59 124629.0 60 124629.0 61 124629.0 62 101596.5 63 78564.0 64 78564.0 65 78564.0 66 60492.5 67 42421.0 68 42421.0 69 42421.0 70 30985.0 71 19549.0 72 19549.0 73 19549.0 74 13688.5 75 7828.0 76 7828.0 77 7828.0 78 5323.0 79 2818.0 80 2818.0 81 2818.0 82 1872.0 83 926.0 84 926.0 85 926.0 86 590.5 87 255.0 88 255.0 89 255.0 90 155.5 91 56.0 92 56.0 93 56.0 94 31.5 95 7.0 96 7.0 97 7.0 98 4.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07616340968352471 2 1.0880487097646387E-4 3 0.0 4 0.026765998260210113 5 0.07224643432837202 6 0.07594579994157179 7 0.03585120498674484 8 0.06789423948931346 9 0.04961502116526753 10 0.10983851725074029 11 0.004025780226129163 12 0.013872621049499143 13 0.0052770362423584976 14 0.007181121484446616 15 0.0069635117424936875 16 0.01849682806599886 17 0.029866937083039335 18 7.072316613470151E-4 19 0.0034817558712468438 20 0.005222633806870266 21 1.0880487097646387E-4 22 2.1760974195292774E-4 23 0.0 24 0.0 25 0.003427353435758612 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1838153.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.022863527078 #Duplication Level Percentage of deduplicated Percentage of total 1 81.83543344880505 58.12186821513755 2 12.349128210262245 17.541408951116892 3 3.2651321211798106 6.956970991212971 4 1.1276334558188594 3.2035102816476075 5 0.48834326205938794 1.7341768427806004 6 0.2789567088318181 1.188738255679503 7 0.1523688124093232 0.7575168558671217 8 0.10405343674641868 0.5912138430051469 9 0.07224483859036847 0.4617931780565567 >10 0.2929200033709079 3.5282072373129947 >50 0.01670881512722927 0.816933148139504 >100 0.013325393080365506 1.8676803779318887 >500 0.0023733939850014633 1.134063414453653 >1k 0.0013780997332266535 2.095918407658036 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4280 0.23284242388963267 No Hit GTCCTACAGTGGACATTTCTAAATT 3326 0.18094250043385943 No Hit TATCAACGCAGAGTACTTTTTTTTT 3076 0.16734189156180143 No Hit CTGTAGGACGTGGAATATGGCAAGA 3022 0.1644041600454369 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2904 0.15798467265782554 No Hit CTTTAGGACGTGAAATATGGCGAGG 2772 0.15080355117337893 No Hit GTACATGGGAAGCAGTGGTATCAAC 2676 0.14558091736650866 No Hit CCCATGTACTCTGCGTTGATACCAC 2646 0.1439488443018617 No Hit GGTATCAACGCAGAGTACTTTTTTT 2583 0.14052149086610308 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.4402435488231934E-5 2 0.0 0.0 0.0 0.0 5.4402435488231934E-5 3 0.0 0.0 0.0 0.0 5.4402435488231934E-5 4 0.0 0.0 0.0 0.0 5.4402435488231934E-5 5 0.0 0.0 0.0 0.0 5.4402435488231934E-5 6 0.0 0.0 0.0 0.0 1.0880487097646387E-4 7 0.0 0.0 0.0 0.0 1.0880487097646387E-4 8 0.0 0.0 0.0 0.0 1.0880487097646387E-4 9 0.0 0.0 0.0 5.4402435488231934E-5 1.0880487097646387E-4 10 0.0 0.0 0.0 5.4402435488231934E-5 1.0880487097646387E-4 11 0.0 0.0 0.0 5.4402435488231934E-5 1.0880487097646387E-4 12 0.0 0.0 0.0 5.4402435488231934E-5 1.0880487097646387E-4 13 0.0 0.0 0.0 1.0880487097646387E-4 1.0880487097646387E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAATC 35 0.0021568034 16.301043 2 TAGGACC 510 0.0 12.3157625 4 CCCCTAA 125 1.8111677E-8 11.408865 1 GGCGAGG 690 0.0 10.588911 19 ACCGTGC 90 9.475587E-5 10.560021 8 GTTGGAC 100 2.374241E-5 10.459836 3 GTGTACG 100 2.378531E-5 10.458126 1 AATGTCC 475 0.0 10.404401 8 GTCCTAC 1475 0.0 10.248641 1 AGGACCT 1245 0.0 10.242902 5 CTATTAG 130 3.8007602E-7 10.238725 1 TGGCGAG 1665 0.0 10.143868 18 CTAGACT 180 3.8198777E-10 10.045433 4 TGTAGGA 1645 0.0 10.000286 2 TGTCCAC 625 0.0 9.729199 10 GTAGGAC 1620 0.0 9.626336 3 GGACCTG 1240 0.0 9.51387 6 TCCTACA 1745 0.0 9.481695 2 GTCCTAA 955 0.0 9.45761 1 AGGACGT 1670 0.0 9.345767 5 >>END_MODULE