FastQCFastQC Report
Thu 26 May 2016
SRR1512956_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512956_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567608
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33040.12868007889054717No Hit
GTACATGGGAAGCAGTGGTATCAAC31160.12135808892946275No Hit
GTCCTACAGTGGACATTTCTAAATT27030.10527307906814434No Hit
CCCATGTACTCTGCGTTGATACCAC26780.10429941019034059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4900.012.5975184
GTCCTAA8550.011.5888871
TATTCCG600.00589400411.079145
GTCCTAC12850.010.5283361
AGGACGT13450.010.23776155
GTAGGAC13500.010.200243
ACCGATC750.002660771410.1277238
TAGATAG1701.5716068E-910.0550185
TCCTAAA9950.010.0217232
CTAGGAC3700.010.0101163
TTAGGAC8600.09.8280583
GCACCGT1451.6327067E-79.8215716
TAGGACG14400.09.6944364
GTAAGAC2450.09.6905873
GGACGTG14050.09.5955486
TGCACCG1701.6761987E-89.4964075
AGACCGT900.00112028879.4941856
TGTAGGA15100.09.4338842
GTATAAT3650.09.396891
GTCTTAT2355.456968E-129.3246921
Produced by FastQC (version 0.11.5)