##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512956_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2567608
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.14894758078336	31.0	30.0	34.0	23.0	34.0
2	29.231773697542614	31.0	30.0	34.0	22.0	34.0
3	29.369428666681205	31.0	30.0	34.0	23.0	34.0
4	32.814496215933275	35.0	33.0	37.0	26.0	37.0
5	32.935999186791754	35.0	35.0	37.0	26.0	37.0
6	32.89924123931691	37.0	35.0	37.0	26.0	37.0
7	32.79775417431321	37.0	35.0	37.0	25.0	37.0
8	32.78359196575178	37.0	35.0	37.0	25.0	37.0
9	34.10213630741141	39.0	35.0	39.0	25.0	39.0
10	33.98163232082156	38.0	34.0	39.0	25.0	39.0
11	34.06753133655916	39.0	35.0	39.0	25.0	39.0
12	33.95946538568192	39.0	34.0	39.0	25.0	39.0
13	33.96404941875863	39.0	35.0	39.0	25.0	39.0
14	34.964728650167785	39.0	35.0	41.0	24.0	41.0
15	34.91098797012628	39.0	35.0	41.0	23.0	41.0
16	34.78944059996697	39.0	34.0	41.0	22.0	41.0
17	34.72472199806201	39.0	34.0	41.0	22.0	41.0
18	34.41528652348801	39.0	34.0	41.0	17.0	41.0
19	34.65819626671984	39.0	34.0	41.0	19.0	41.0
20	34.741934905951375	39.0	34.0	41.0	20.0	41.0
21	34.68246048462226	39.0	34.0	41.0	19.0	41.0
22	34.58505620795698	39.0	34.0	41.0	18.0	41.0
23	34.314816358260295	39.0	34.0	41.0	17.0	41.0
24	34.32116623721378	39.0	34.0	41.0	16.0	41.0
25	34.24166967854906	39.0	34.0	41.0	16.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5534174615577463
1101	2	0.8082807826528686
1101	3	0.8191076610140513
1101	4	0.8121231790653312
1101	5	0.7060046447704025
1101	6	0.8178794383225636
1101	7	0.7467565387391488
1101	8	0.6286334183575377
1101	9	0.525118644633686
1101	10	0.6799906334501884
1101	11	0.8009601525950032
1101	12	0.9961648653291135
1101	13	0.8565416983594716
1101	14	0.8448554483728259
1101	15	0.6267830083572221
1101	16	1.0840585636728548
1101	17	1.0227194046541896
1101	18	1.2704772736625145
1101	19	0.707828790909069
1101	20	0.9205078099280755
1101	21	0.8681375578154942
1101	22	0.9807932877037899
1101	23	1.162980757407027
1101	24	0.785315685731284
1101	25	1.20633289354123
1102	1	0.6481671934499715
1102	2	0.7098667499443003
1102	3	0.7014632088808206
1102	4	0.817397674595064
1102	5	0.6258635451937664
1102	6	0.5969222539050563
1102	7	0.7720316225385488
1102	8	0.7651733245207879
1102	9	0.7247787253891786
1102	10	0.9275952728853909
1102	11	0.8574731299400398
1102	12	0.8604340234726422
1102	13	0.8710086815306965
1102	14	0.9615544118184474
1102	15	1.1303443619812157
1102	16	1.0633810782625233
1102	17	0.9017806423658357
1102	18	1.188476158272259
1102	19	1.2226617010365572
1102	20	1.157565006381894
1102	21	1.0330742472272973
1102	22	1.3845117325509904
1102	23	1.4792610431358
1102	24	1.5157159529295612
1102	25	1.297888940353559
1103	1	0.594786890091715
1103	2	0.6944438307557235
1103	3	0.6056409604962738
1103	4	0.7356332217878361
1103	5	0.3439435690757904
1103	6	0.661914506370465
1103	7	0.49162469659765407
1103	8	0.5281554553733798
1103	9	0.8281099171275841
1103	10	0.7236444310782488
1103	11	0.8291597943696729
1103	12	0.9695694719077323
1103	13	0.9801557732180655
1103	14	1.071782226364725
1103	15	1.1157565121158015
1103	16	1.0241740486394164
1103	17	0.9335581317981507
1103	18	1.1553877531573207
1103	19	1.2379679968293757
1103	20	1.1130923636346637
1103	21	0.8718760916711545
1103	22	1.093807781518315
1103	23	1.3428500355669186
1103	24	1.2196200308546068
1103	25	1.0654109852293274
1104	1	0.5440401662459919
1104	2	0.5522413792887733
1104	3	0.6154911180612821
1104	4	0.8662312435785964
1104	5	0.589723850354531
1104	6	0.5958072261193195
1104	7	0.7956263801586942
1104	8	0.8217579859166833
1104	9	0.9053117696449391
1104	10	0.9015796654951203
1104	11	0.9614872049792638
1104	12	0.8385258016046038
1104	13	0.9395175430278542
1104	14	0.9756526911971335
1104	15	1.1654298782650656
1104	16	1.0417773524404126
1104	17	1.119290277035553
1104	18	1.2842024931694311
1104	19	1.113632999691582
1104	20	1.1584863661860183
1104	21	1.311401000847141
1104	22	1.0672159823012208
1104	23	1.4229988701660474
1104	24	1.3573883796568467
1104	25	1.0631441047914194
1105	1	0.7117125022629835
1105	2	0.815003949026373
1105	3	0.8544690430238795
1105	4	0.9930587538574827
1105	5	0.721876067002384
1105	6	0.3723612309602089
1105	7	0.8530446978322814
1105	8	0.8721839868430621
1105	9	0.8096402926130253
1105	10	0.8955609709717507
1105	11	0.8913184649550772
1105	12	0.8762957513376222
1105	13	0.9306175371433909
1105	14	1.080594150558568
1105	15	1.1882800898737713
1105	16	1.194866964019468
1105	17	1.1437220295970647
1105	18	1.154480059130961
1105	19	1.0658683142332066
1105	20	1.128887395991434
1105	21	1.0672395599985691
1105	22	0.8338471590643834
1105	23	1.070416872862161
1105	24	1.258084101408187
1105	25	1.2174785488476374
1106	1	0.6984868991519306
1106	2	0.734972505712765
1106	3	0.6995452721100825
1106	4	0.7133057787098238
1106	5	0.7410952263290795
1106	6	0.6975102328021094
1106	7	0.53856088385497
1106	8	0.6741670977173086
1106	9	0.7041630274692707
1106	10	0.8951131041637126
1106	11	0.8393025829695802
1106	12	0.9265526798770978
1106	13	0.8853740285631702
1106	14	1.0652013855359428
1106	15	1.1398143258309759
1106	16	1.0390407993099586
1106	17	1.1179499195110765
1106	18	1.3260466502409187
1106	19	0.9185852225100035
1106	20	0.9340247074016332
1106	21	0.883267899910642
1106	22	1.0121970826024906
1106	23	1.1547319015393782
1106	24	1.0400952710138398
1106	25	1.1192163774147375
1107	1	0.47905464521488383
1107	2	0.48818280269303216
1107	3	0.6257546679680424
1107	4	0.6187160234171927
1107	5	0.594288749171163
1107	6	0.3616927666332046
1107	7	0.632071632956773
1107	8	0.5822619847259389
1107	9	0.7170705317505792
1107	10	0.5273654808250399
1107	11	0.49724045624292046
1107	12	0.6539230021355991
1107	13	0.5460644208829564
1107	14	0.8045796501538192
1107	15	0.9742762075772049
1107	16	0.8387731932915514
1107	17	0.941787920345881
1107	18	1.2845841014301271
1107	19	1.2421169977989663
1107	20	1.0895646591077153
1107	21	1.01149097254266
1107	22	1.0173736596640524
1107	23	1.0987148621357576
1107	24	0.9548881104073175
1107	25	0.9941858523842129
1108	1	0.39090542585671173
1108	2	0.5492853154131545
1108	3	0.3525471057796956
1108	4	0.5719356085025069
1108	5	0.41432421790524643
1108	6	0.5408269814278412
1108	7	0.6478634467539379
1108	8	0.6607999553096349
1108	9	0.8371828959398115
1108	10	0.7941558811595897
1108	11	0.7370509768123767
1108	12	0.7288319468085902
1108	13	0.7799733266478626
1108	14	0.9267891307189302
1108	15	0.9087185699932974
1108	16	0.7980548370781833
1108	17	0.8885269620010021
1108	18	1.0993458309571196
1108	19	1.0734252392750534
1108	20	0.9303321404979172
1108	21	0.8499154146645012
1108	22	0.8225774831116155
1108	23	1.0549960090274624
1108	24	1.042763725697185
1108	25	1.102637966771617
1109	1	-0.09911315541043919
1109	2	-0.05451823481224238
1109	3	0.13186944569079984
1109	4	0.2876504871312662
1109	5	0.18300400392926264
1109	6	0.09642088565090745
1109	7	0.15362905444032293
1109	8	0.2591955247966382
1109	9	0.3708721570863105
1109	10	0.4331712950277975
1109	11	0.08045155415482697
1109	12	0.25607883147685584
1109	13	0.33105605788149006
1109	14	0.2083640022760207
1109	15	0.3852164506770137
1109	16	0.3918599872057271
1109	17	0.21001768505434626
1109	18	-1.4299028781147243
1109	19	0.015767605037360966
1109	20	0.2589589901863647
1109	21	0.3909243329659233
1109	22	0.5514391727987658
1109	23	0.027869611187085752
1109	24	0.371800104812543
1109	25	0.4866325121295674
1110	1	0.31492968563871315
1110	2	0.30633585694292975
1110	3	0.3941720335974388
1110	4	0.2713799650026303
1110	5	0.35371961747950564
1110	6	0.3895032865933956
1110	7	0.4055260093458557
1110	8	0.3042719874402664
1110	9	0.625595055242087
1110	10	0.7988460993342699
1110	11	0.5988367160196617
1110	12	0.12708722276864393
1110	13	0.3585247249059904
1110	14	0.45577158205008317
1110	15	0.5471400601182381
1110	16	0.7656250973995071
1110	17	0.32846153856755933
1110	18	0.9060851894022335
1110	19	0.6848742568438269
1110	20	0.6029180050362655
1110	21	0.6456836649892068
1110	22	0.6976567742831961
1110	23	0.5894272800675466
1110	24	0.7254380626153747
1110	25	0.6245496354992781
1111	1	0.2532817700123502
1111	2	0.46123628068947653
1111	3	0.3997635910263959
1111	4	0.3658824184923333
1111	5	0.29310256737661433
1111	6	0.47422265330757796
1111	7	0.44027787538355057
1111	8	0.2026605975199871
1111	9	0.6328929433904804
1111	10	0.5322086309153349
1111	11	0.37041603436146175
1111	12	0.4783114569423077
1111	13	0.5571555688562384
1111	14	0.4882539817368965
1111	15	0.5598293954117608
1111	16	0.36055669074045227
1111	17	0.4380390287642939
1111	18	0.7596027878556555
1111	19	0.4136913465596237
1111	20	0.4205014873829498
1111	21	0.5477008547601514
1111	22	0.3729988540725202
1111	23	0.7613452608878291
1111	24	0.8726241977251519
1111	25	0.20659085338236594
1112	1	-0.14073873033817463
1112	2	0.22761780771890017
1112	3	0.23242161351284452
1112	4	0.34032192496351144
1112	5	0.21568725026181568
1112	6	0.22668173882178166
1112	7	-0.030876011917385426
1112	8	0.33689730306532795
1112	9	0.4661645415695119
1112	10	0.49693866390368413
1112	11	0.5149339400734405
1112	12	0.5313017416791865
1112	13	0.25142118482959575
1112	14	0.3847060668244282
1112	15	0.42034123710797644
1112	16	0.608267077979086
1112	17	0.5621646362042299
1112	18	0.4152238963698238
1112	19	0.3805689574028648
1112	20	0.38871323203980523
1112	21	0.41915120924956994
1112	22	0.42900900104120154
1112	23	0.5679736404312834
1112	24	0.3970089543653046
1112	25	0.4957727902174227
1113	1	-0.24942477252073303
1113	2	0.058248733089541815
1113	3	-0.10777993333515568
1113	4	0.10672718580852347
1113	5	-0.07029478483386242
1113	6	0.17967239948887226
1113	7	0.008513923448653316
1113	8	0.32683531772902086
1113	9	0.1516473415340016
1113	10	-0.13318241939228415
1113	11	0.044513385334028044
1113	12	0.12801878697518987
1113	13	-0.023293677400758384
1113	14	0.1277213385847631
1113	15	-0.36845232192818145
1113	16	-0.1446336661483656
1113	17	-0.14139684713640577
1113	18	-0.2327198985185035
1113	19	-0.09687990979298178
1113	20	-0.26261887819978824
1113	21	-0.07625342263975199
1113	22	-0.07340516665195906
1113	23	-0.6628948779863748
1113	24	0.01605485917737326
1113	25	0.07820229507834142
1114	1	-1.5044437887183335
1114	2	-1.2505356768950122
1114	3	-1.1174294908682896
1114	4	-1.399917516955476
1114	5	-1.3784691209780142
1114	6	-1.8079881804390467
1114	7	-2.0268167250620195
1114	8	-1.610577639418036
1114	9	-1.800239018602447
1114	10	-1.9066760332164776
1114	11	-2.54748428761226
1114	12	-2.0730420135250682
1114	13	-1.7839350482469456
1114	14	-2.0980014402533556
1114	15	-2.090948615908488
1114	16	-2.6947746115605966
1114	17	-4.840092421534457
1114	18	-3.8586765027000425
1114	19	-2.756540962598123
1114	20	-1.903597734054678
1114	21	-2.9690897993424556
1114	22	-3.1672459719120987
1114	23	-2.935214107445386
1114	24	-2.434241793505194
1114	25	-2.2800573922938163
1115	1	-1.0610731862007299
1115	2	-0.8238314833624045
1115	3	-0.8241962221657282
1115	4	-0.8054067676855325
1115	5	-0.7119755112206079
1115	6	-0.7644895995288437
1115	7	-1.7553010992978457
1115	8	-1.2382770732019956
1115	9	-0.9635166032942664
1115	10	-0.7372475343594758
1115	11	-1.5997249665035582
1115	12	-1.1631364039001824
1115	13	-1.0536422534998664
1115	14	-1.102102175908243
1115	15	-2.291596017451738
1115	16	-1.1285540917903703
1115	17	-1.2397745580415886
1115	18	-1.152625453330863
1115	19	-1.2485848778660795
1115	20	-1.0955132607428695
1115	21	-1.7804911091015967
1115	22	-1.8246774114954718
1115	23	-1.2095297338406112
1115	24	-1.1172885374868784
1115	25	-1.3832552429131226
1116	1	-1.31794230343613
1116	2	-1.9823045129738688
1116	3	-1.6099112624048821
1116	4	-1.2675383649165894
1116	5	-1.8985379840619991
1116	6	-1.631504248491975
1116	7	-1.3184532359990975
1116	8	-1.982835268367534
1116	9	-2.040533374530078
1116	10	-2.8819973942750785
1116	11	-1.6181310604991097
1116	12	-1.5436307253803179
1116	13	-2.0274065578337677
1116	14	-1.9399525609662902
1116	15	-1.6344714352441656
1116	16	-1.5395329156383752
1116	17	-2.0033465398169312
1116	18	-1.6763311070748728
1116	19	-2.163385413043713
1116	20	-2.5036517443166275
1116	21	-3.016679591402948
1116	22	-2.1484990099893366
1116	23	-2.2414576937267796
1116	24	-2.769028452970492
1116	25	-2.483026606444504
1201	1	0.2796499146499869
1201	2	0.292560794083343
1201	3	0.44273203807683004
1201	4	0.6477127966644787
1201	5	0.5698727362541192
1201	6	0.5955106143114222
1201	7	0.5427635420059715
1201	8	0.18396806440942726
1201	9	0.3473066087012313
1201	10	0.6467941926562162
1201	11	0.20326347725567473
1201	12	-0.4176918534628271
1201	13	-0.17737472837754353
1201	14	0.40819182055243886
1201	15	0.11453195342843259
1201	16	0.5719007490577113
1201	17	0.7896479715426921
1201	18	1.042595386289463
1201	19	0.544283836208102
1201	20	0.6594339467901449
1201	21	0.5558343145759395
1201	22	0.7360532233128154
1201	23	1.0549987562019965
1201	24	0.8280902049133161
1201	25	0.7620650585077726
1202	1	0.6279868736025556
1202	2	0.7749621399674176
1202	3	0.4781153710713184
1202	4	0.8182563345911689
1202	5	0.7656668485195581
1202	6	0.7688069121136891
1202	7	0.7898283152184078
1202	8	0.7609929896945644
1202	9	1.0322777392845168
1202	10	1.1831259469815407
1202	11	0.8490758870387438
1202	12	0.9785627463297999
1202	13	0.8707863710999604
1202	14	1.1180206371096588
1202	15	0.8311745622478384
1202	16	1.0642005437189042
1202	17	1.1706642969945378
1202	18	1.588935882762108
1202	19	1.330437698000857
1202	20	1.071932676129201
1202	21	1.194319585074247
1202	22	1.347500353652137
1202	23	1.3640931307318525
1202	24	1.4240230733541637
1202	25	1.219749507650839
1203	1	0.5372526466110941
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2211	13	0.06123118315206
2211	14	0.27350494324680596
2211	15	0.22687019531299057
2211	16	0.18180226998277504
2211	17	0.3100713973839859
2211	18	0.3686251186400682
2211	19	0.06979382843510962
2211	20	-0.09193664545153979
2211	21	-0.08466370166379988
2211	22	-0.036012849702729
2211	23	0.47992830549470256
2211	24	0.27618487091493193
2211	25	0.30599433435298806
2212	1	0.007833173449931508
2212	2	-0.041292311564692596
2212	3	0.003338436856157756
2212	4	-0.10868375072170977
2212	5	0.03795239229132363
2212	6	-0.013689761466714856
2212	7	-0.1392235573601539
2212	8	-0.11462337628985608
2212	9	0.09276916886226161
2212	10	0.16129827508392225
2212	11	-0.21154272917070216
2212	12	0.12910084361779184
2212	13	0.030421247631089443
2212	14	-0.08968834030982009
2212	15	-0.11969226356517737
2212	16	0.02912149233550565
2212	17	0.06139826169535212
2212	18	-2.5988586032082317
2212	19	-0.9615466091255129
2212	20	-0.3029836742014851
2212	21	-0.4366293250341542
2212	22	-0.11829622600583178
2212	23	-0.17431772122291278
2212	24	-0.5438931204391295
2212	25	-0.23723695186468063
2213	1	0.04732315065197312
2213	2	0.1896793995873267
2213	3	-0.0833630166373851
2213	4	-0.13282403232496165
2213	5	6.312366542218228E-4
2213	6	-0.01730631776857905
2213	7	0.0915317659536754
2213	8	-0.21184367616311306
2213	9	-0.09580226606468756
2213	10	-0.1922788346171771
2213	11	-0.12071730163479799
2213	12	-0.22103346266578683
2213	13	-0.009231236713851843
2213	14	0.012525686664140778
2213	15	-0.3298880235004802
2213	16	-0.702745429267587
2213	17	-0.258707443331069
2213	18	-0.09740518381078545
2213	19	0.023721963896178977
2213	20	-0.7737470944491562
2213	21	-0.4589629968107545
2213	22	-0.31793013386885605
2213	23	-0.746010064430088
2213	24	-0.41135587849800004
2213	25	-0.6936131142541768
2214	1	-2.049669247342166
2214	2	-4.13178611141527
2214	3	-2.369757797977371
2214	4	-2.0564838559009537
2214	5	-2.0227899248240924
2214	6	-2.0966324116497326
2214	7	-2.2446853130187563
2214	8	-1.9360518731866527
2214	9	-2.2286470675573042
2214	10	-2.308481735434004
2214	11	-2.1230463436637343
2214	12	-2.5932202160213436
2214	13	-2.3979206976005756
2214	14	-3.847822253937629
2214	15	-3.6647518651361892
2214	16	-2.9786980835250247
2214	17	-3.5629073257432395
2214	18	-2.9052429728214477
2214	19	-4.950316533705315
2214	20	-3.279219999856455
2214	21	-3.2679144630401744
2214	22	-3.253699893088484
2214	23	-3.2895909890000823
2214	24	-3.6169141750697307
2214	25	-3.3889470536631627
2215	1	-1.0095572470267733
2215	2	-2.684065678811347
2215	3	-1.3433081304958456
2215	4	-1.0230262633341667
2215	5	-0.9433289467042485
2215	6	-1.3746006636711137
2215	7	-1.8526800340090475
2215	8	-1.589584076049345
2215	9	-1.485258012864719
2215	10	-3.0520117863925194
2215	11	-2.1029820274641366
2215	12	-2.2186505626339716
2215	13	-1.6331014424934338
2215	14	-3.0747623830902064
2215	15	-2.6695406732632705
2215	16	-2.3345225571366797
2215	17	-2.641316170562199
2215	18	-1.534114723439295
2215	19	-2.2441246895316525
2215	20	-2.3980234535257665
2215	21	-1.8751690905239329
2215	22	-1.631088787346485
2215	23	-2.0703071624216562
2215	24	-1.8979619634953835
2215	25	-1.8840145114785827
2216	1	-2.0029921867180533
2216	2	-1.9997270601598842
2216	3	-2.5087918788791868
2216	4	-3.278568569994963
2216	5	-2.2638464843627055
2216	6	-2.193555730756522
2216	7	-2.170328093360947
2216	8	-2.6758565608641334
2216	9	-2.8638045283955513
2216	10	-2.5057851195237397
2216	11	-3.0334478379989065
2216	12	-3.390763417493872
2216	13	-3.982762611360929
2216	14	-3.380595893108282
2216	15	-3.244005991115017
2216	16	-2.391158858963962
2216	17	-2.4794719637841567
2216	18	-2.5302397990429775
2216	19	-2.832186628213087
2216	20	-2.8467947547004435
2216	21	-3.1914616809794687
2216	22	-3.825637290408398
2216	23	-3.6100794061118116
2216	24	-3.535699054895108
2216	25	-3.486608827499307
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	119476.0
3	6.0
4	942.0
5	5718.0
6	7227.0
7	6488.0
8	5769.0
9	5537.0
10	5109.0
11	4857.0
12	4675.0
13	4569.0
14	4568.0
15	4717.0
16	4981.0
17	5121.0
18	5757.0
19	6967.0
20	8912.0
21	11715.0
22	14746.0
23	17899.0
24	20759.0
25	23524.0
26	26199.0
27	29615.0
28	33570.0
29	39791.0
30	49118.0
31	61028.0
32	77682.0
33	98747.0
34	128641.0
35	167977.0
36	229040.0
37	351174.0
38	974987.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82618307664225	18.005443260671836	12.439957426400088	25.728416236285824
2	15.32005816264032	19.450938129725127	40.55632440789279	24.67267929974176
3	18.85245199069619	23.827030143881807	29.310240036778882	28.010277828643115
4	12.73082167764055	16.158881053492813	37.26617836131999	33.84411890754666
5	13.667730481670134	37.41724782967323	35.454495511287725	13.460526177368912
6	33.08460027353365	36.25637527788674	17.51258110670111	13.1464433418785
7	28.788868568000638	31.26812081125141	21.564072150190192	18.37893847055776
8	25.956489151504954	36.01526611691816	20.045446187600117	17.982798543976763
9	26.60872498698903	15.10133755917266	19.54540284153414	38.74453461230417
10	15.175150024249294	28.52098781302528	34.01227462277252	22.291587539952904
11	34.9199974290105	22.198663475003944	23.892127513288315	18.98921158269724
12	23.433367756321086	24.38218787627825	31.00951546950371	21.17492889789695
13	28.983618792966976	21.382554863721072	25.528302886383326	24.10552345692863
14	21.465912495290972	21.31806758324019	27.076266547819355	30.13975337364948
15	23.681520364585808	29.762067087466466	22.995701359405157	23.56071118854257
16	23.13646159096115	27.680935173888184	26.451743088959635	22.73086014619104
17	22.451558616606025	27.979135339810362	27.15428785128281	22.4150181923008
18	22.504896030267858	27.181340851431653	28.315020899996217	21.998742218304272
19	23.73809777955074	26.91719147722713	27.006557956928006	22.338152786294128
20	23.986318686562747	26.81514060159602	27.262354377961866	21.936186333879366
21	23.63430953378069	26.447714678068117	26.73810103528541	23.179874752865786
22	23.760605917771976	27.012821177048952	26.582604911960317	22.64396799321876
23	23.1277750984992	26.96910744184126	26.795716132209023	23.10740132745052
24	23.493197990424246	26.532765067630553	27.217231523865955	22.75680541807925
25	23.307108453822863	26.68202294638002	26.968190452149333	23.042678147647784
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	201.0
1	201.0
2	417.0
3	633.0
4	633.0
5	633.0
6	1139.0
7	1645.0
8	1645.0
9	1645.0
10	2573.5
11	3502.0
12	3502.0
13	3502.0
14	6139.0
15	8776.0
16	8776.0
17	8776.0
18	16676.5
19	24577.0
20	24577.0
21	24577.0
22	39168.5
23	53760.0
24	53760.0
25	53760.0
26	77767.5
27	101775.0
28	101775.0
29	101775.0
30	132630.0
31	163485.0
32	163485.0
33	163485.0
34	190250.5
35	217016.0
36	217016.0
37	217016.0
38	242032.5
39	267049.0
40	267049.0
41	267049.0
42	288075.0
43	309101.0
44	309101.0
45	309101.0
46	326935.0
47	344769.0
48	344769.0
49	344769.0
50	338244.5
51	331720.0
52	331720.0
53	331720.0
54	303461.5
55	275203.0
56	275203.0
57	275203.0
58	239298.5
59	203394.0
60	203394.0
61	203394.0
62	167972.5
63	132551.0
64	132551.0
65	132551.0
66	102764.0
67	72977.0
68	72977.0
69	72977.0
70	53668.0
71	34359.0
72	34359.0
73	34359.0
74	24088.0
75	13817.0
76	13817.0
77	13817.0
78	9424.0
79	5031.0
80	5031.0
81	5031.0
82	3341.5
83	1652.0
84	1652.0
85	1652.0
86	1067.0
87	482.0
88	482.0
89	482.0
90	290.5
91	99.0
92	99.0
93	99.0
94	60.5
95	22.0
96	22.0
97	22.0
98	17.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35889434835847217
2	0.03941411617349689
3	0.03575312119295469
4	0.03536365364183318
5	0.04124461366376799
6	0.018110241127150248
7	0.01803234761692595
8	0.02399120114908506
9	0.021187034781010188
10	0.02036915292365501
11	0.01959021782141199
12	0.020447046433879314
13	0.020252312658318557
14	0.028392184476758134
15	0.029210066334113306
16	0.021654395842355997
17	0.02290069200594483
18	0.10807724543621924
19	0.025315390822898198
20	0.0234848933326271
21	0.026600633741599183
22	0.020524939944103617
23	0.022667011475271927
24	0.01943443080096339
25	0.022589117965047624
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2567608.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.30634981700666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.03871042584431	54.671521532498204
2	12.744095844521459	17.410053377146873
3	3.6884624254779412	7.558362141647433
4	1.430402953205532	3.908224180037459
5	0.6950642271654057	2.3738650123023795
6	0.3818008268650276	1.5647652504158984
7	0.24007687242701392	1.1479142381680747
8	0.16074131664570113	0.8783722083877997
9	0.11282345828850576	0.6935902748477221
>10	0.4745097562955237	5.348199532949525
>50	0.01972197804779568	0.9290850787295593
>100	0.010728816981855835	1.4295457566267522
>500	0.002059990728441475	0.9598797681035008
>1k	8.011075054738696E-4	1.1266216481386833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3304	0.12868007889054717	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3116	0.12135808892946275	No Hit
GTCCTACAGTGGACATTTCTAAATT	2703	0.10527307906814434	No Hit
CCCATGTACTCTGCGTTGATACCAC	2678	0.10429941019034059	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	3.8946755112151074E-5
3	0.0	0.0	0.0	0.0	3.8946755112151074E-5
4	0.0	0.0	0.0	0.0	3.8946755112151074E-5
5	0.0	0.0	0.0	0.0	3.8946755112151074E-5
6	0.0	0.0	0.0	0.0	3.8946755112151074E-5
7	0.0	0.0	0.0	0.0	3.8946755112151074E-5
8	0.0	0.0	0.0	0.0	3.8946755112151074E-5
9	0.0	0.0	0.0	3.8946755112151074E-5	3.8946755112151074E-5
10	0.0	0.0	0.0	3.8946755112151074E-5	3.8946755112151074E-5
11	0.0	0.0	0.0	3.8946755112151074E-5	3.8946755112151074E-5
12	0.0	0.0	0.0	3.8946755112151074E-5	3.8946755112151074E-5
13	0.0	0.0	0.0	3.8946755112151074E-5	3.8946755112151074E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	490	0.0	12.597518	4
GTCCTAA	855	0.0	11.588887	1
TATTCCG	60	0.005894004	11.07914	5
GTCCTAC	1285	0.0	10.528336	1
AGGACGT	1345	0.0	10.2377615	5
GTAGGAC	1350	0.0	10.20024	3
ACCGATC	75	0.0026607714	10.127723	8
TAGATAG	170	1.5716068E-9	10.055018	5
TCCTAAA	995	0.0	10.021723	2
CTAGGAC	370	0.0	10.010116	3
TTAGGAC	860	0.0	9.828058	3
GCACCGT	145	1.6327067E-7	9.821571	6
TAGGACG	1440	0.0	9.694436	4
GTAAGAC	245	0.0	9.690587	3
GGACGTG	1405	0.0	9.595548	6
TGCACCG	170	1.6761987E-8	9.496407	5
AGACCGT	90	0.0011202887	9.494185	6
TGTAGGA	1510	0.0	9.433884	2
GTATAAT	365	0.0	9.39689	1
GTCTTAT	235	5.456968E-12	9.324692	1
>>END_MODULE