FastQCFastQC Report
Thu 26 May 2016
SRR1512956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512956_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567608
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40920.1593701219189222No Hit
GTACATGGGAAGCAGTGGTATCAAC31580.1229938526441731No Hit
CCCATGTACTCTGCGTTGATACCAC30800.11995600574542531No Hit
TATCAACGCAGAGTACTTTTTTTTT29720.115749756193313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGT751.457518E-512.6850984
AACACCG907.3458705E-611.6280065
TACGGTG600.005814153711.0994625
GGCGAGG7000.010.70963819
CTTACAC2900.010.48961453
GTAGGAC13700.010.4776523
TGTAGGA14300.010.4364842
AAGTCGT1106.020193E-610.3677857
TAGGACC6150.010.3646534
CAACCGT750.00262445910.1445136
TGGCGAG13850.010.1404618
TAAGACG856.5084663E-410.0734614
GTCCTAA8700.010.0541161
GTCTTAG3550.09.6416091
TAATACC2106.548362E-119.51382354
TACGCTG1002.7164453E-49.51382355
ACAACCG1106.7314104E-59.5138235
GGACTTA1304.1816147E-69.5104816
ATAATAC2700.09.5062133
GTAGAAC5600.09.5062133
Produced by FastQC (version 0.11.5)