##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512956_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2567608 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.54940629566507 31.0 31.0 34.0 27.0 34.0 2 30.674622450155944 31.0 31.0 34.0 27.0 34.0 3 30.771970254026314 31.0 31.0 34.0 27.0 34.0 4 34.26810751485429 37.0 35.0 37.0 30.0 37.0 5 33.93687665718443 37.0 35.0 37.0 30.0 37.0 6 33.819435832884146 37.0 35.0 37.0 29.0 37.0 7 33.69865571380055 37.0 35.0 37.0 28.0 37.0 8 33.58926946792501 37.0 35.0 37.0 28.0 37.0 9 35.05981053182573 39.0 35.0 39.0 28.0 39.0 10 34.94448140058763 39.0 35.0 39.0 28.0 39.0 11 34.99296855283205 39.0 35.0 39.0 28.0 39.0 12 34.837399244744525 39.0 35.0 39.0 27.0 39.0 13 34.83084801106711 39.0 35.0 39.0 27.0 39.0 14 35.90575819984982 40.0 36.0 41.0 27.0 41.0 15 35.922434421453744 40.0 36.0 41.0 27.0 41.0 16 35.893082978398574 40.0 36.0 41.0 27.0 41.0 17 35.86887718062882 40.0 36.0 41.0 27.0 41.0 18 35.78964779670417 40.0 36.0 41.0 27.0 41.0 19 35.76695313303277 40.0 36.0 41.0 27.0 41.0 20 35.74582841305994 40.0 36.0 41.0 27.0 41.0 21 35.690163763315894 40.0 36.0 41.0 27.0 41.0 22 35.65329988066714 40.0 36.0 41.0 27.0 41.0 23 35.54862385535487 40.0 36.0 41.0 27.0 41.0 24 35.53103238500581 40.0 36.0 41.0 27.0 41.0 25 35.437685191820556 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6932598458008172 1101 2 0.7075522205738629 1101 3 0.6721666442838305 1101 4 0.4395458130174461 1101 5 0.7806497063707312 1101 6 0.7194194034235508 1101 7 0.719608695291555 1101 8 0.7822691793379377 1101 9 0.6713439280982669 1101 10 0.89096800542751 1101 11 0.9014663788555382 1101 12 0.9671944374817585 1101 13 0.9177931790339855 1101 14 1.1225515422432295 1101 15 1.089194914358508 1101 16 1.2194387427560613 1101 17 1.123557963292214 1101 18 0.6262920550982187 1101 19 1.030237552351572 1101 20 0.8127426853610729 1101 21 1.0574740924530346 1101 22 0.9748559430360544 1101 23 1.0216913586115552 1101 24 1.0126532360134135 1101 25 1.0426643994543738 1102 1 0.6106271145537505 1102 2 0.4528755734478125 1102 3 0.49458235046480326 1102 4 0.5185738444542665 1102 5 0.6102183073222562 1102 6 0.6063282359443392 1102 7 0.6135810210737489 1102 8 0.6399286246073785 1102 9 0.7936676397393114 1102 10 0.9292231434019058 1102 11 0.8370394328536648 1102 12 0.8919588103224285 1102 13 0.9424513537183401 1102 14 1.0822422981658875 1102 15 1.0020973324177689 1102 16 1.0123889112837077 1102 17 1.0153076359704585 1102 18 1.047856744056439 1102 19 1.0961411713324054 1102 20 1.0417663241263426 1102 21 1.0841604429324931 1102 22 1.06074215387887 1102 23 1.0755747318272455 1102 24 1.0508075554028267 1102 25 1.0885604638141757 1103 1 0.5123364966864372 1103 2 0.3914060728517157 1103 3 0.4829242100945166 1103 4 0.5115892291988615 1103 5 0.545278354055597 1103 6 0.5983209232184166 1103 7 0.5557760909771687 1103 8 0.5298367116286684 1103 9 0.6383737583256419 1103 10 0.7050636239742829 1103 11 0.7009097720693234 1103 12 0.6737442209837567 1103 13 0.7583352693478957 1103 14 0.8727992769976893 1103 15 0.5369986491741656 1103 16 0.7294607440423206 1103 17 0.9402867258346035 1103 18 0.8796840895132618 1103 19 0.9577609271447685 1103 20 0.8874407413815604 1103 21 0.497320104751374 1103 22 0.7738684510994105 1103 23 0.8209347049217897 1103 24 0.8513952915079415 1103 25 0.9519360602445417 1104 1 0.5233955571183273 1104 2 0.545479980481435 1104 3 0.5147413131441105 1104 4 0.551742172667268 1104 5 0.6027019904824371 1104 6 0.6349298731509805 1104 7 0.7373558524326711 1104 8 0.644272659908431 1104 9 0.8053190803542094 1104 10 0.8447411715170006 1104 11 0.9002810250393765 1104 12 0.8739466884560443 1104 13 0.8713944463186252 1104 14 1.0224131358161372 1104 15 0.95238026259684 1104 16 0.9543366214284603 1104 17 0.9016266548659573 1104 18 1.0037351795390137 1104 19 1.0826016553174327 1104 20 1.111097337442203 1104 21 0.9471748313390052 1104 22 1.059390476314313 1104 23 0.8559422461849167 1104 24 0.959789726453387 1104 25 1.07608737116891 1105 1 0.5737454384890199 1105 2 0.6375793321894285 1105 3 0.5897381816820371 1105 4 0.5525923066303662 1105 5 0.5759901916949275 1105 6 0.6974152993386937 1105 7 0.7275275786386217 1105 8 0.8193131491636692 1105 9 0.9336822278923407 1105 10 0.8983459421804341 1105 11 0.8801380325922423 1105 12 0.7878240342751823 1105 13 0.9427502729501427 1105 14 1.0388088119812053 1105 15 0.9640999272752424 1105 16 1.0824983298858015 1105 17 0.8597382526050552 1105 18 1.1029232945844072 1105 19 1.0482052608083947 1105 20 1.0997512506954479 1105 21 0.9917968229684178 1105 22 1.0585975388417381 1105 23 0.9613326094857939 1105 24 1.0294709839020513 1105 25 1.007682871239595 1106 1 0.47514329515844267 1106 2 0.46280217120483513 1106 3 0.4330377170255204 1106 4 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11 0.006169563380879595 1113 12 0.016441594680685512 1113 13 0.0851682035156216 1113 14 0.08079081707548852 1113 15 0.30479573844071695 1113 16 -0.10476637521804832 1113 17 0.082367691521533 1113 18 0.16536590248156102 1113 19 0.03568741962698141 1113 20 0.08918194139604907 1113 21 -0.009294517772183042 1113 22 -0.1546536996060297 1113 23 -0.033554784917825486 1113 24 -0.16746511769036942 1113 25 -0.1468558642304032 1114 1 -0.7621250678162674 1114 2 -0.5647133109515678 1114 3 -0.6193827504081426 1114 4 -0.33624008638018665 1114 5 -0.6070019781477143 1114 6 -0.8674402245412196 1114 7 -0.7320276463884952 1114 8 -1.174202984755432 1114 9 -0.9751647022483283 1114 10 -1.0591572827671172 1114 11 -0.9372521699941458 1114 12 -1.0249537664559654 1114 13 -0.8675303854747369 1114 14 -2.054292784195681 1114 15 -1.2889189143756283 1114 16 -1.059957649388842 1114 17 -1.2010762857492523 1114 18 -1.4410355485048711 1114 19 -1.3645086377591582 1114 20 -0.7193148534607872 1114 21 -0.7683242483721671 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2216 15 -1.8551363827105547 2216 16 -1.6042199558102013 2216 17 -1.8762487635086345 2216 18 -1.5978889278679063 2216 19 -1.7730070287632458 2216 20 -2.043012572883974 2216 21 -1.966267626178869 2216 22 -2.1843609644645596 2216 23 -1.270841905259978 2216 24 -1.641005658739708 2216 25 -1.570566986636905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60527.0 3 0.0 4 1010.0 5 8382.0 6 9645.0 7 7684.0 8 6099.0 9 5503.0 10 4766.0 11 4478.0 12 4023.0 13 3858.0 14 3737.0 15 3856.0 16 3877.0 17 4131.0 18 4630.0 19 5592.0 20 7191.0 21 9167.0 22 11708.0 23 14588.0 24 17375.0 25 20120.0 26 22936.0 27 25161.0 28 29101.0 29 35251.0 30 44483.0 31 57318.0 32 72480.0 33 93885.0 34 122313.0 35 159912.0 36 219622.0 37 342281.0 38 1120918.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.22212841492035 18.82381234693786 13.44557467951229 27.508484558629497 2 14.196876702704115 20.041384838100225 39.092119627341724 26.669618831853942 3 17.360710825016902 23.77263195939567 29.777442662587124 29.089214553000303 4 11.942595259021012 16.658446621912223 36.52364567945098 34.87531243961578 5 13.311032331386185 37.61304448819941 35.547687976166536 13.528235204247869 6 33.29752581201285 36.465088251378155 17.12793536248684 13.109450574122153 7 28.625421694304308 31.287253153722112 21.609682985621 18.477642166352584 8 25.909007294195042 36.3277492754911 19.754150239528332 18.009093190785524 9 26.304889605147086 14.838000268077295 19.410042923538747 39.44706720323688 10 14.861819429498278 28.736801883996943 34.103970741901776 22.297407944603005 11 34.92240797777788 22.103210398156673 23.827901658090337 19.146479965975107 12 23.18780798178956 24.44803314274541 31.05453587964721 21.30962299581782 13 28.831021534336575 21.287869316267578 25.612107742958955 24.269001406436892 14 21.366823280779386 21.21803792624375 27.040881307846636 30.37425748513023 15 23.543538818192015 29.796654743060834 23.043354978624755 23.61645146012239 16 23.038293410118037 27.69866366882234 26.43108502799572 22.8319578930639 17 22.387298574580242 28.062408522677025 27.134243616699255 22.416049286043478 18 22.44483053946718 27.25319356018428 28.218223399461518 22.08375250088702 19 23.623014495937174 26.873548955481635 27.117361434863707 22.386075113717492 20 23.88893194838417 26.792059379000722 27.2410941820217 22.077914490593404 21 23.445039248638324 26.323869909896576 26.84252445372244 23.388566387742664 22 23.55386578304131 27.01674401504282 26.634675752179852 22.794714449736016 23 22.916270707989696 26.898849045492923 26.93584846284947 23.249031783667913 24 23.344421478832235 26.636046715872013 27.19984016245477 22.81969164284098 25 23.23137274452314 26.59760672522364 27.054154813501547 23.11686571675168 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 129.0 1 129.0 2 281.0 3 433.0 4 433.0 5 433.0 6 1034.5 7 1636.0 8 1636.0 9 1636.0 10 2445.5 11 3255.0 12 3255.0 13 3255.0 14 6042.0 15 8829.0 16 8829.0 17 8829.0 18 16739.5 19 24650.0 20 24650.0 21 24650.0 22 39362.5 23 54075.0 24 54075.0 25 54075.0 26 78232.0 27 102389.0 28 102389.0 29 102389.0 30 132924.0 31 163459.0 32 163459.0 33 163459.0 34 190742.5 35 218026.0 36 218026.0 37 218026.0 38 242572.5 39 267119.0 40 267119.0 41 267119.0 42 288840.0 43 310561.0 44 310561.0 45 310561.0 46 328103.0 47 345645.0 48 345645.0 49 345645.0 50 339238.5 51 332832.0 52 332832.0 53 332832.0 54 303663.5 55 274495.0 56 274495.0 57 274495.0 58 238568.0 59 202641.0 60 202641.0 61 202641.0 62 167022.5 63 131404.0 64 131404.0 65 131404.0 66 101874.0 67 72344.0 68 72344.0 69 72344.0 70 53010.5 71 33677.0 72 33677.0 73 33677.0 74 23361.0 75 13045.0 76 13045.0 77 13045.0 78 8992.0 79 4939.0 80 4939.0 81 4939.0 82 3211.5 83 1484.0 84 1484.0 85 1484.0 86 964.5 87 445.0 88 445.0 89 445.0 90 266.0 91 87.0 92 87.0 93 87.0 94 48.0 95 9.0 96 9.0 97 9.0 98 4.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08042504930659197 2 3.8946755112151074E-5 3 0.0 4 0.027730089639851567 5 0.07025994622232054 6 0.07941243367367605 7 0.034234197743580795 8 0.06745577985424567 9 0.046112958052786875 10 0.11092035855940627 11 0.0029599533885234816 12 0.01125561222741166 13 0.004946237899243187 14 0.005880960021934813 15 0.005919906777046963 16 0.016747104698224963 17 0.02784692990518802 18 7.399883471308704E-4 19 0.002375752061841216 20 0.003933622266327259 21 1.1684026533645323E-4 22 1.557870204486043E-4 23 0.0 24 3.8946755112151074E-5 25 0.003115740408972086 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2567608.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.08395324166118 #Duplication Level Percentage of deduplicated Percentage of total 1 78.9309378276532 52.949993425507515 2 13.499202949603037 18.111597989417294 3 3.851805774498461 7.751830754172458 4 1.5231824602671586 4.08724403772322 5 0.7288494150305752 2.4447050039061606 6 0.4014840139305425 1.6159880890674574 7 0.24191951857861116 1.1360242370800913 8 0.1753841262884692 0.9412368421812213 9 0.1249177645444415 0.7541979728176939 >10 0.4851976825360249 5.4235254507820105 >50 0.022615972882674258 1.014893896830385 >100 0.011595650123287487 1.5382446608814355 >500 0.001976653963257429 0.9068790508330229 >1k 9.301901003228882E-4 1.3236385888001365 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4092 0.1593701219189222 No Hit GTACATGGGAAGCAGTGGTATCAAC 3158 0.1229938526441731 No Hit CCCATGTACTCTGCGTTGATACCAC 3080 0.11995600574542531 No Hit TATCAACGCAGAGTACTTTTTTTTT 2972 0.115749756193313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.8946755112151074E-5 0.0 4 0.0 0.0 0.0 3.8946755112151074E-5 0.0 5 0.0 0.0 0.0 3.8946755112151074E-5 0.0 6 0.0 0.0 0.0 3.8946755112151074E-5 0.0 7 0.0 0.0 0.0 3.8946755112151074E-5 0.0 8 0.0 0.0 0.0 3.8946755112151074E-5 0.0 9 0.0 0.0 0.0 7.789351022430215E-5 0.0 10 0.0 0.0 0.0 7.789351022430215E-5 0.0 11 0.0 0.0 0.0 7.789351022430215E-5 0.0 12 0.0 0.0 0.0 7.789351022430215E-5 0.0 13 0.0 0.0 0.0 7.789351022430215E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGGT 75 1.457518E-5 12.685098 4 AACACCG 90 7.3458705E-6 11.628006 5 TACGGTG 60 0.0058141537 11.099462 5 GGCGAGG 700 0.0 10.709638 19 CTTACAC 290 0.0 10.4896145 3 GTAGGAC 1370 0.0 10.477652 3 TGTAGGA 1430 0.0 10.436484 2 AAGTCGT 110 6.020193E-6 10.367785 7 TAGGACC 615 0.0 10.364653 4 CAACCGT 75 0.002624459 10.144513 6 TGGCGAG 1385 0.0 10.14046 18 TAAGACG 85 6.5084663E-4 10.073461 4 GTCCTAA 870 0.0 10.054116 1 GTCTTAG 355 0.0 9.641609 1 TAATACC 210 6.548362E-11 9.5138235 4 TACGCTG 100 2.7164453E-4 9.5138235 5 ACAACCG 110 6.7314104E-5 9.513823 5 GGACTTA 130 4.1816147E-6 9.510481 6 ATAATAC 270 0.0 9.506213 3 GTAGAAC 560 0.0 9.506213 3 >>END_MODULE