FastQCFastQC Report
Thu 26 May 2016
SRR1512955_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512955_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249609
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT33270.147892367073567No Hit
GTCCTAAAGTGTGTATTTCTCATTT31050.13802398550148048No Hit
CTGTAGGACGTGGAATATGGCAAGA28960.12873348212956118No Hit
GTATCAACGCAGAGTACTTTTTTTT28770.12788889091393216No Hit
CTTTAGGACGTGAAATATGGCGAGG26720.11877619621898738No Hit
GTCCTACAGTGTGCATTTCTCATTT22690.10086197201380329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6900.013.0770774
GTCCTAT3100.011.6802221
CGTGCGC600.00590048711.07740610
GTATTAT2250.011.0108291
AGGACCT13100.010.9479345
GGCGAGG7300.010.92541819
GGACCTG12050.010.8736096
CTAGGAC3450.010.7369693
TGGCGAG18300.010.22903918
GTATCAA18100.010.1603341
GTAGGAC20400.010.1499173
GGTATCA9050.010.1076891
GTCCTAC18500.010.0951691
TGTAGGA21450.010.0075312
GCCTCGA2002.5465852E-119.97720716
CTGTAGG21250.09.9097471
TCCAACG1252.5636455E-69.88218718
ACTGTTC5350.09.7611338
TCGAACT1951.6552804E-109.73816219
GTATAAT2750.09.701851
Produced by FastQC (version 0.11.5)