##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512955_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2249609
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.062165025122145	31.0	30.0	34.0	23.0	34.0
2	29.085900705411472	31.0	30.0	34.0	22.0	34.0
3	29.263443113892237	31.0	30.0	34.0	23.0	34.0
4	32.73141288108289	35.0	33.0	37.0	25.0	37.0
5	32.84254463775705	35.0	35.0	37.0	26.0	37.0
6	32.93698371583684	37.0	35.0	37.0	26.0	37.0
7	32.7361608172798	36.0	33.0	37.0	25.0	37.0
8	32.73498283479485	36.0	35.0	37.0	25.0	37.0
9	34.087530321935944	39.0	35.0	39.0	25.0	39.0
10	33.95805093240648	38.0	34.0	39.0	24.0	39.0
11	34.04800123043604	39.0	35.0	39.0	25.0	39.0
12	33.947603783590836	39.0	34.0	39.0	24.0	39.0
13	33.94312833919139	39.0	35.0	39.0	23.0	39.0
14	34.925267013067604	39.0	35.0	41.0	23.0	41.0
15	34.884299893892674	39.0	35.0	41.0	22.0	41.0
16	34.761612351301935	39.0	34.0	41.0	21.0	41.0
17	34.6986245165271	39.0	34.0	41.0	19.0	41.0
18	34.39426762606302	39.0	34.0	41.0	17.0	41.0
19	34.64140790688515	39.0	34.0	41.0	18.0	41.0
20	34.733036274303664	39.0	34.0	41.0	19.0	41.0
21	34.6652071537765	39.0	34.0	41.0	18.0	41.0
22	34.576421058059424	39.0	34.0	41.0	18.0	41.0
23	34.303149125025726	39.0	34.0	41.0	16.0	41.0
24	34.312920600868864	39.0	34.0	41.0	16.0	41.0
25	34.22679807913286	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.726173316386582
1101	2	0.9331529113713373
1101	3	0.9516220718481563
1101	4	0.9296805566870603
1101	5	0.7378902078065934
1101	6	0.9545363711070181
1101	7	0.8854523194717459
1101	8	0.7849558338413871
1101	9	0.8219613588789514
1101	10	0.8536375276649792
1101	11	1.0636571759454156
1101	12	1.1571021118441465
1101	13	1.0008360385974342
1101	14	1.0824677651419279
1101	15	0.8500346319186107
1101	16	1.2793329350018183
1101	17	1.3226188395752843
1101	18	1.5865577214781226
1101	19	0.960081042388218
1101	20	1.2174171491285577
1101	21	1.1924707452160987
1101	22	1.2633934672332998
1101	23	1.446351976567371
1101	24	1.0982364843482983
1101	25	1.2797522057048312
1102	1	0.6699994132276004
1102	2	0.6593879626299142
1102	3	0.6146818214129297
1102	4	0.7781916477686863
1102	5	0.6826537998565811
1102	6	0.6363212915118268
1102	7	0.8505857647319104
1102	8	0.804718280280369
1102	9	0.8503901302918209
1102	10	1.0871472723889681
1102	11	0.9461106249630191
1102	12	0.9799029812718558
1102	13	0.9901156606042392
1102	14	0.9728413884923057
1102	15	1.0908560430623524
1102	16	1.1507393904203553
1102	17	1.0396595260033834
1102	18	1.3459082578654815
1102	19	1.2489208768205344
1102	20	1.2289846439978689
1102	21	0.8556188402365024
1102	22	1.3030521064913643
1102	23	1.489415092238005
1102	24	1.4051334353458813
1102	25	1.2341579884951202
1103	1	0.398418185909442
1103	2	0.44982282730603984
1103	3	0.503742965695448
1103	4	0.7286855195780788
1103	5	0.20661088023529572
1103	6	0.5373209879597738
1103	7	0.3702038851213061
1103	8	0.4963043269128349
1103	9	0.7284948465378775
1103	10	0.7502247424099835
1103	11	0.7191961241310096
1103	12	0.7512884395097075
1103	13	0.7685888975142134
1103	14	0.9739749981296697
1103	15	0.9996912340507151
1103	16	1.1141196571460767
1103	17	0.8764422327829706
1103	18	1.2596181280054637
1103	19	1.2880182489661394
1103	20	1.0420415659434852
1103	21	0.983942089892011
1103	22	1.13633479247801
1103	23	1.4032848314679427
1103	24	1.245587569808734
1103	25	1.188803144274928
1104	1	0.6324080764200062
1104	2	0.5731092341107811
1104	3	0.5657862449952269
1104	4	0.8481707772725358
1104	5	0.3828641068741092
1104	6	0.5963423957099678
1104	7	0.7843369484159766
1104	8	0.7499684277066834
1104	9	0.7860574225586205
1104	10	0.9746309947975789
1104	11	0.87864754925819
1104	12	0.8005955005537118
1104	13	0.7238410627471481
1104	14	0.9953906577028775
1104	15	0.9902553463661619
1104	16	0.9283953990925937
1104	17	1.013266395817915
1104	18	1.0906875990058893
1104	19	0.9024200191011928
1104	20	1.0770108820424227
1104	21	1.1009934608114094
1104	22	0.9323774616956797
1104	23	1.1612718149152457
1104	24	1.1114576506955771
1104	25	0.7799341388188452
1105	1	0.584886495675395
1105	2	0.7239031977544172
1105	3	0.6739156585408246
1105	4	0.837431980979936
1105	5	0.5083255647700682
1105	6	0.37705750661737625
1105	7	0.6374676891250672
1105	8	0.7223282084007892
1105	9	0.6316960305280972
1105	10	0.9076171546552629
1105	11	0.8811441998464957
1105	12	0.9036938093613145
1105	13	0.8414662785626703
1105	14	0.9428298945362101
1105	15	1.1067330463845693
1105	16	1.1948127668887736
1105	17	1.042224327724007
1105	18	1.1078282830209503
1105	19	1.0918001909393809
1105	20	1.1571649331706055
1105	21	1.1143845646640997
1105	22	0.8073484022272837
1105	23	0.9551006420048225
1105	24	1.0172253271319889
1105	25	1.0850533872452672
1106	1	0.5719416271375586
1106	2	0.6499487058605098
1106	3	0.701713454041414
1106	4	0.6104457998291295
1106	5	0.7478018936363924
1106	6	0.6908291870709391
1106	7	0.5352909956297367
1106	8	0.6850530017098748
1106	9	0.7137116652049826
1106	10	0.8347831013003884
1106	11	0.82813911035052
1106	12	0.8543974888287238
1106	13	0.8539978376351272
1106	14	1.0194097860497138
1106	15	1.0177046597436927
1106	16	0.9444657557996976
1106	17	1.084822783426155
1106	18	1.2259112797429381
1106	19	0.7885001962947129
1106	20	0.908538543859791
1106	21	0.8323321341873324
1106	22	0.9512671806453241
1106	23	1.2240947448667754
1106	24	1.1486902229257367
1106	25	1.2040454235598972
1107	1	0.47536279319745844
1107	2	0.5199575946678046
1107	3	0.6285461384857491
1107	4	0.6721864123568224
1107	5	0.5259313919976236
1107	6	0.5245163027950142
1107	7	0.7285923657626654
1107	8	0.7246250886867713
1107	9	0.9675434153472864
1107	10	0.7213248709793874
1107	11	0.7881801509542399
1107	12	0.908861829581582
1107	13	0.8183940218971841
1107	14	0.9884745526391896
1107	15	1.0796111312591137
1107	16	0.9345524369035871
1107	17	0.9995089874797145
1107	18	1.3814223600491502
1107	19	1.1894480772443643
1107	20	1.170672976525907
1107	21	1.1680997277625451
1107	22	1.1250395254158363
1107	23	1.175671517043554
1107	24	1.184842180461402
1107	25	1.1297198646819737
1108	1	0.6909898308866858
1108	2	0.7848917277616216
1108	3	0.6230958446817674
1108	4	0.812570387176045
1108	5	0.7389190285904945
1108	6	0.9100106868175573
1108	7	0.9315295736186542
1108	8	0.9394008687028261
1108	9	1.058882280573556
1108	10	0.956950342090785
1108	11	0.8649831988428645
1108	12	0.9180234844026387
1108	13	0.8889815388148108
1108	14	1.1736843155271188
1108	15	1.1168304618877158
1108	16	1.008025015669645
1108	17	1.0783161718872734
1108	18	1.2392573840203056
1108	19	1.2064873581631588
1108	20	1.1351856719282338
1108	21	1.1006141291335965
1108	22	1.0309271267528572
1108	23	1.2254229209201881
1108	24	1.3238340515235691
1108	25	1.5075920156058942
1109	1	0.1444451823665709
1109	2	0.012147026644935721
1109	3	0.28536233691051294
1109	4	0.3840304502477281
1109	5	0.3512729239410035
1109	6	0.21845254661455016
1109	7	0.34250380350986376
1109	8	0.325927086324441
1109	9	0.42483616796828017
1109	10	0.6110212723207695
1109	11	0.22660836652205063
1109	12	0.46330073240871883
1109	13	0.4848443103887945
1109	14	0.22225713636066757
1109	15	0.6239613853552939
1109	16	0.5846430783921619
1109	17	0.40625909356037937
1109	18	-1.1782472181794716
1109	19	0.20901994925135625
1109	20	0.3969922756705486
1109	21	0.4692652481366508
1109	22	0.5377039073566579
1109	23	0.15365843863175144
1109	24	0.6116787192701594
1109	25	0.6388961453205226
1110	1	0.43288939954052097
1110	2	0.402281113224511
1110	3	0.4561828401211656
1110	4	0.33749123874086706
1110	5	0.5710918235105922
1110	6	0.531971540592977
1110	7	0.65083004023505
1110	8	0.4621691032696944
1110	9	0.8751702011112314
1110	10	0.9702962880484804
1110	11	0.860429815835225
1110	12	0.30054506956331295
1110	13	0.5522817514607397
1110	14	0.5855116236855054
1110	15	0.7781924421370121
1110	16	0.9526187048451931
1110	17	0.5102732005818993
1110	18	1.0036154084778488
1110	19	0.8429205696755346
1110	20	0.751475812378871
1110	21	0.6635163719287362
1110	22	0.7718387565198057
1110	23	0.7249028713804222
1110	24	0.9392895512725516
1110	25	1.0528801804892254
1111	1	-0.02833628634722629
1111	2	0.16646767675078067
1111	3	0.2134405325857358
1111	4	0.08443074645322923
1111	5	0.13100749063173822
1111	6	0.3539351550108236
1111	7	0.19628120886081035
1111	8	0.10776746635013978
1111	9	0.24775488742309193
1111	10	0.28503866211247697
1111	11	0.17064404970135882
1111	12	0.26024847887517666
1111	13	0.1607916263989111
1111	14	0.23121199723548358
1111	15	0.4433551143836283
1111	16	0.17901082501163046
1111	17	0.21983268246692234
1111	18	0.46546733410366414
1111	19	0.16936183386087578
1111	20	0.152320249976853
1111	21	0.3960935500166016
1111	22	0.10758863445176559
1111	23	0.42326987226073953
1111	24	0.48042642152323367
1111	25	-0.032330083443063984
1112	1	0.017630937368092958
1112	2	0.2599314147021623
1112	3	0.2618108116489246
1112	4	0.37857951279590196
1112	5	0.38753465938312814
1112	6	0.3841406635282141
1112	7	0.17097395645108548
1112	8	0.3639418151690279
1112	9	0.6214029161983277
1112	10	0.6503616762373952
1112	11	0.5837428433239253
1112	12	0.5659938261380901
1112	13	0.30252864824303316
1112	14	0.5792621922361931
1112	15	0.5850218147291741
1112	16	0.6428764867005583
1112	17	0.7413437863932089
1112	18	0.4916281440133332
1112	19	0.5777895017332426
1112	20	0.5282278620770029
1112	21	0.5560256533972918
1112	22	0.5080348122358345
1112	23	0.7973014361057906
1112	24	0.4322226508359819
1112	25	0.7299654689242274
1113	1	-0.22697432052794753
1113	2	-0.07412349967907161
1113	3	-0.25798987778009774
1113	4	0.05671677475493908
1113	5	-0.27263185932023504
1113	6	-0.1660120186066223
1113	7	-0.2530749594177806
1113	8	-0.06390727825580456
1113	9	-0.1113694362582649
1113	10	-0.4025637582548498
1113	11	-0.17768720202482768
1113	12	0.05374278439644087
1113	13	-0.04660732603534257
1113	14	0.03272509343669583
1113	15	-0.39099197385125706
1113	16	-0.3080561620025648
1113	17	-0.3981445005116413
1113	18	-0.28595789464907995
1113	19	-0.2054864858218437
1113	20	-0.2617387861942291
1113	21	-0.2671612362540756
1113	22	-0.19503834562102185
1113	23	-0.45971162465514226
1113	24	0.1061785497730412
1113	25	0.03913071594296724
1114	1	-1.498164695113175
1114	2	-1.2341470052059726
1114	3	-1.1976753060810559
1114	4	-1.3450467371690529
1114	5	-1.1879204139628357
1114	6	-1.6536659658414088
1114	7	-1.875357223692042
1114	8	-1.4001549061884049
1114	9	-1.5145713256130762
1114	10	-1.5439121727135756
1114	11	-2.3689153520810926
1114	12	-1.93627971845617
1114	13	-1.7368076138561435
1114	14	-2.1071773549130626
1114	15	-2.0524855839420653
1114	16	-2.7233660295284636
1114	17	-4.641086101435327
1114	18	-3.710570512812467
1114	19	-2.637613590691082
1114	20	-1.8885707859384695
1114	21	-2.7863290262873015
1114	22	-2.789869051091987
1114	23	-2.6913821009997
1114	24	-2.1960502449979344
1114	25	-2.073082583393088
1115	1	-1.170816636051363
1115	2	-0.6543911159776776
1115	3	-0.8565266480316502
1115	4	-0.8077602043604983
1115	5	-0.6600664429901073
1115	6	-0.8496113189722294
1115	7	-1.982198826958939
1115	8	-1.3486712131029748
1115	9	-1.0652324149461876
1115	10	-0.902920850158182
1115	11	-1.700155092403925
1115	12	-1.2756308149520876
1115	13	-1.0960220357968566
1115	14	-1.2763595405000885
1115	15	-2.4541520644629102
1115	16	-1.2982529927205775
1115	17	-1.3006211238638912
1115	18	-1.4154896607973981
1115	19	-1.5317500638262445
1115	20	-1.2102335939949072
1115	21	-2.0656433133131813
1115	22	-2.045931465689101
1115	23	-1.4767260573133427
1115	24	-1.3771606215976888
1115	25	-1.6529652125615897
1116	1	-1.3489312250064316
1116	2	-2.008708261570259
1116	3	-1.5574576583060917
1116	4	-1.2421315878385855
1116	5	-1.7853012963793446
1116	6	-1.5694723955795737
1116	7	-1.2554903804770383
1116	8	-1.8920956649417668
1116	9	-1.877961021963415
1116	10	-2.794546489633703
1116	11	-1.5082527564953523
1116	12	-1.4634524189755496
1116	13	-1.978413854752624
1116	14	-1.8734213286941284
1116	15	-1.4450616708425486
1116	16	-1.3542696818027906
1116	17	-1.800330639520709
1116	18	-1.6229262394702744
1116	19	-2.0129712973710028
1116	20	-2.3263528439162826
1116	21	-2.9687776153083902
1116	22	-2.0117616794057867
1116	23	-2.0416106977854227
1116	24	-2.5816799558782257
1116	25	-2.2495603330283522
1201	1	0.31817551105111974
1201	2	0.23648285688209114
1201	3	0.4888739159642199
1201	4	0.6159519038913857
1201	5	0.7111487970392503
1201	6	0.7278586463160863
1201	7	0.6591086280510794
1201	8	0.18707188732912527
1201	9	0.5658613065987836
1201	10	0.637585863158634
1201	11	0.28574942563345473
1201	12	-0.20187428236780391
1201	13	0.07017353059958253
1201	14	0.6482349839940014
1201	15	0.37792395909026055
1201	16	0.7944895613750163
1201	17	0.8890095195439258
1201	18	1.2025228883866887
1201	19	0.6662169021298183
1201	20	0.7633557265059565
1201	21	0.6272184414755415
1201	22	0.8402296649515435
1201	23	1.1115249484425078
1201	24	0.882011485440124
1201	25	0.851532958038888
1202	1	0.4109957653353291
1202	2	0.5890822606533668
1202	3	0.20224351589029155
1202	4	0.6372165424782636
1202	5	0.6646461744084675
1202	6	0.6000871440028703
1202	7	0.609615950541837
1202	8	0.6784843289212361
1202	9	0.8050464716755954
1202	10	0.9531218873048246
1202	11	0.7110451677880505
1202	12	0.7888401937124954
1202	13	0.7801841663431048
1202	14	1.042665534404037
1202	15	0.6621693617509337
1202	16	0.8982781633894561
1202	17	1.0007354061515557
1202	18	1.3042950722456013
1202	19	1.0076366459973514
1202	20	0.914482215943238
1202	21	0.9936885380388532
1202	22	0.9859122874338055
1202	23	1.0457948983002154
1202	24	1.235413644438296
1202	25	1.0638824672187113
1203	1	0.505203184478006
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2211	15	0.3046895464095485
2211	16	0.15180241838810105
2211	17	0.43424465236502385
2211	18	0.4599646883430353
2211	19	0.24141916415980091
2211	20	0.1471036543554547
2211	21	0.08464947220934249
2211	22	0.1605083165297927
2211	23	0.7148602013281078
2211	24	0.3305596339353727
2211	25	0.5479320506291288
2212	1	-0.06104437892317094
2212	2	-0.05936947729037456
2212	3	-0.038989068683939365
2212	4	-0.10448349352598285
2212	5	-0.1350739267339094
2212	6	-0.14698349899865804
2212	7	-0.22855083905194817
2212	8	-0.12630846234851134
2212	9	-0.08239131930974253
2212	10	-0.00799400283423779
2212	11	-0.1835135513886783
2212	12	-0.04939237592616763
2212	13	-0.13496243576801703
2212	14	-0.16894807551902602
2212	15	-0.2583206773389364
2212	16	0.04706452156148089
2212	17	-0.0011722151498645417
2212	18	-2.872916845318972
2212	19	-1.1154195457320952
2212	20	-0.4800833354603711
2212	21	-0.533056452429399
2212	22	-0.20039904411636655
2212	23	-0.19928883779024176
2212	24	-0.7432029154178892
2212	25	-0.3549646596392151
2213	1	-0.23389586785737038
2213	2	-0.1331253858975856
2213	3	-0.3370534953709985
2213	4	-0.4585707272627957
2213	5	-0.418118922356463
2213	6	-0.46636369900029706
2213	7	-0.29692166187950875
2213	8	-0.707405065662968
2213	9	-0.5592397392815514
2213	10	-0.6275794017138878
2213	11	-0.5910227604914979
2213	12	-0.6558216875556226
2213	13	-0.4500985988180375
2213	14	-0.556438603705935
2213	15	-0.776907587277428
2213	16	-1.2706015730509321
2213	17	-0.7413349815713133
2213	18	-0.612762776823125
2213	19	-0.5215731065324292
2213	20	-1.227158863713754
2213	21	-0.8306305921165631
2213	22	-0.8116750437289468
2213	23	-1.1568888243698652
2213	24	-0.9538788277980004
2213	25	-1.2073844744506346
2214	1	-1.9092463429110929
2214	2	-3.8287645786855506
2214	3	-2.0301475776912667
2214	4	-1.764761362330372
2214	5	-1.701578943395372
2214	6	-1.7700239924574959
2214	7	-1.860654987336119
2214	8	-1.731024664205144
2214	9	-1.8884313591633841
2214	10	-1.9929082336785306
2214	11	-1.6964371578449189
2214	12	-2.1458157867258123
2214	13	-2.0273004508327475
2214	14	-3.152542411318745
2214	15	-3.3474832571961493
2214	16	-2.643944188387316
2214	17	-3.0052571470080274
2214	18	-2.475682942422292
2214	19	-4.715449266978613
2214	20	-2.8162785122686564
2214	21	-2.7286989598423155
2214	22	-2.800663685806363
2214	23	-3.004832529513653
2214	24	-3.0784642089117185
2214	25	-2.916460138663208
2215	1	-1.1327759483909041
2215	2	-2.5866891272630816
2215	3	-1.443785926675762
2215	4	-1.208618411592532
2215	5	-1.1110017050020176
2215	6	-1.6613366447659992
2215	7	-2.037391087614477
2215	8	-2.0069749132717973
2215	9	-1.6646575401067523
2215	10	-3.2745802560581296
2215	11	-2.245291832567684
2215	12	-2.412381925447445
2215	13	-1.8018301360416231
2215	14	-3.1230506320781117
2215	15	-2.9607141213551635
2215	16	-2.7001479758643043
2215	17	-2.7466481417840427
2215	18	-1.724260991972173
2215	19	-2.402836476357521
2215	20	-2.5159464234019566
2215	21	-2.2301517619721167
2215	22	-1.8221142693884147
2215	23	-2.449774587286022
2215	24	-2.267783114751701
2215	25	-2.1522567532979977
2216	1	-1.9986763315326677
2216	2	-2.02738281550668
2216	3	-2.5237768522655983
2216	4	-3.2212772137703887
2216	5	-2.290559075439603
2216	6	-2.3212025451491414
2216	7	-2.12363831487804
2216	8	-2.7332453306946647
2216	9	-2.9362294296006013
2216	10	-2.488721211896003
2216	11	-3.123840935209344
2216	12	-3.5905781343605305
2216	13	-4.146506482079129
2216	14	-3.3607548063067867
2216	15	-3.20155904976815
2216	16	-2.4831183327911077
2216	17	-2.6509534452726484
2216	18	-2.558269864728775
2216	19	-2.8696947139054174
2216	20	-2.80184579827592
2216	21	-2.9983308303326766
2216	22	-3.676752301249678
2216	23	-3.576261782285016
2216	24	-3.7032136451005577
2216	25	-3.6877931517654297
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107302.0
3	3.0
4	911.0
5	5283.0
6	6479.0
7	5744.0
8	5109.0
9	4788.0
10	4520.0
11	4243.0
12	4126.0
13	4006.0
14	4158.0
15	4180.0
16	4282.0
17	4701.0
18	5194.0
19	6118.0
20	7821.0
21	10176.0
22	12650.0
23	15345.0
24	17709.0
25	20216.0
26	22516.0
27	25236.0
28	28643.0
29	34138.0
30	41843.0
31	52947.0
32	66751.0
33	85766.0
34	111531.0
35	147302.0
36	202617.0
37	320232.0
38	845023.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.144297377149535	18.603650913620744	12.439599839736012	25.812451869493714
2	14.739656385384862	20.420628617118826	41.285650883495315	23.554064114001
3	18.504451719454963	25.026214050731195	29.649981278854355	26.81935295095949
4	12.084317006544381	17.035493167686397	38.7557980948942	32.12439173087501
5	13.000863623143676	37.96526473918314	36.11238841161827	12.921483226054914
6	31.329572029414642	37.4332435244218	18.61840993766728	12.618774508496278
7	27.516650216519505	31.560007469256007	22.90554058740363	18.017801726820853
8	24.664707347004107	36.941549433546164	21.061456239884752	17.33228697956498
9	26.486795549994973	15.10337609585064	20.21442614729193	38.195402206862454
10	14.50947927036228	28.92074457655732	35.21461907520593	21.35515707787447
11	34.039099461272	22.41725565726638	25.066346195314125	18.477298686147485
12	23.02072454315349	24.726732406502137	32.24110771578303	20.01143533456134
13	28.821882531935074	22.098878120980466	25.791924409549644	23.287314937534816
14	20.872171223907674	22.03976460492178	27.941137891093092	29.146926280077455
15	22.788227714200723	30.830684351151653	23.479386405463462	22.901701529184155
16	22.174579574879775	28.050527487693138	27.725693950431097	22.04919898699599
17	21.72365958090089	28.514345674511247	27.86613371511398	21.89586102947388
18	21.613691148180898	27.610799592209617	29.54219700404898	21.233312255560506
19	23.320539817594582	27.079583611968754	27.522124838154323	22.077751732282344
20	23.35263580050402	27.504948628522953	27.972956389822095	21.169459181150927
21	22.890264496719915	26.880258387408595	27.19515025615533	23.034326859716163
22	23.041857181220468	28.023823878298327	27.286574792857106	21.647744147624103
23	22.397052337286315	27.782263514715194	27.541145273851274	22.279538874147217
24	22.89548681969954	27.0133692573771	28.276535108194505	21.81460881472886
25	22.77957429326395	27.379622223398	27.67920779224821	22.161595691089843
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	217.0
1	217.0
2	368.0
3	519.0
4	519.0
5	519.0
6	993.5
7	1468.0
8	1468.0
9	1468.0
10	2481.0
11	3494.0
12	3494.0
13	3494.0
14	6371.5
15	9249.0
16	9249.0
17	9249.0
18	17125.0
19	25001.0
20	25001.0
21	25001.0
22	41092.5
23	57184.0
24	57184.0
25	57184.0
26	82160.5
27	107137.0
28	107137.0
29	107137.0
30	138670.0
31	170203.0
32	170203.0
33	170203.0
34	194804.5
35	219406.0
36	219406.0
37	219406.0
38	238926.0
39	258446.0
40	258446.0
41	258446.0
42	271221.5
43	283997.0
44	283997.0
45	283997.0
46	288369.5
47	292742.0
48	292742.0
49	292742.0
50	281718.0
51	270694.0
52	270694.0
53	270694.0
54	241901.0
55	213108.0
56	213108.0
57	213108.0
58	182644.0
59	152180.0
60	152180.0
61	152180.0
62	123359.5
63	94539.0
64	94539.0
65	94539.0
66	73062.5
67	51586.0
68	51586.0
69	51586.0
70	37762.5
71	23939.0
72	23939.0
73	23939.0
74	16646.0
75	9353.0
76	9353.0
77	9353.0
78	6442.5
79	3532.0
80	3532.0
81	3532.0
82	2306.0
83	1080.0
84	1080.0
85	1080.0
86	728.5
87	377.0
88	377.0
89	377.0
90	238.0
91	99.0
92	99.0
93	99.0
94	69.5
95	40.0
96	40.0
97	40.0
98	29.5
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3692641699068594
2	0.041207160888847794
3	0.0362285179335609
4	0.03600625708734273
5	0.04191839559674592
6	0.017291893835773238
7	0.017291893835773238
8	0.023515197529881862
9	0.01969231097492942
10	0.019470050128711256
11	0.018758815420813126
12	0.019558954467198523
13	0.01938114579022399
14	0.027738153608026995
15	0.028093770961976062
16	0.020314641344340283
17	0.02218163245257287
18	0.10561835412287203
19	0.024582049591729053
20	0.022270536791060135
21	0.02591561466903804
22	0.019425597959467623
23	0.022448345468034665
24	0.01862545891308223
25	0.022314988960303768
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2249609.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.68395635004222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.41952041937569	61.46680301322734
2	11.500140276914511	16.947516683670628
3	2.8218174039466826	6.237680112605905
4	0.9709540095739458	2.8617493143738044
5	0.46012896482743176	1.6952061279867296
6	0.2196388996971937	0.9710317858835594
7	0.14851358237210946	0.766014782462644
8	0.09193936724920733	0.5419565058592876
9	0.054606202420392366	0.36212409320271843
>10	0.2826738722269674	3.5139514266142022
>50	0.016469658246021496	0.8528925535282416
>100	0.010940972149451444	1.6420777345420523
>500	0.0018111620456939702	0.887154949334374
>1k	8.452089546563505E-4	1.2538409167085605
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3327	0.147892367073567	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3105	0.13802398550148048	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2896	0.12873348212956118	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2877	0.12788889091393216	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2672	0.11877619621898738	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2269	0.10086197201380329	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.4452169243633004E-5	0.0	0.0	0.0
6	0.0	4.4452169243633004E-5	0.0	0.0	0.0
7	0.0	4.4452169243633004E-5	0.0	0.0	0.0
8	0.0	4.4452169243633004E-5	0.0	0.0	0.0
9	0.0	4.4452169243633004E-5	0.0	0.0	0.0
10	0.0	4.4452169243633004E-5	0.0	0.0	0.0
11	0.0	4.4452169243633004E-5	0.0	0.0	0.0
12	0.0	4.4452169243633004E-5	0.0	0.0	0.0
13	0.0	4.4452169243633004E-5	0.0	0.0	8.890433848726601E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	690	0.0	13.077077	4
GTCCTAT	310	0.0	11.680222	1
CGTGCGC	60	0.005900487	11.077406	10
GTATTAT	225	0.0	11.010829	1
AGGACCT	1310	0.0	10.947934	5
GGCGAGG	730	0.0	10.925418	19
GGACCTG	1205	0.0	10.873609	6
CTAGGAC	345	0.0	10.736969	3
TGGCGAG	1830	0.0	10.229039	18
GTATCAA	1810	0.0	10.160334	1
GTAGGAC	2040	0.0	10.149917	3
GGTATCA	905	0.0	10.107689	1
GTCCTAC	1850	0.0	10.095169	1
TGTAGGA	2145	0.0	10.007531	2
GCCTCGA	200	2.5465852E-11	9.977207	16
CTGTAGG	2125	0.0	9.909747	1
TCCAACG	125	2.5636455E-6	9.882187	18
ACTGTTC	535	0.0	9.761133	8
TCGAACT	195	1.6552804E-10	9.738162	19
GTATAAT	275	0.0	9.70185	1
>>END_MODULE