FastQCFastQC Report
Thu 26 May 2016
SRR1512955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249609
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT32650.14513633258046177No Hit
GTATCAACGCAGAGTACTTTTTTTT32150.14291372411828013No Hit
GTCCTAAAGTGTGTATTTCTCATTT29540.1313117079456919No Hit
CTGTAGGACGTGGAATATGGCAAGA29380.13060047323779378No Hit
CTTTAGGACGTGAAATATGGCGAGG28930.1286001256218303No Hit
TATCAACGCAGAGTACTTTTTTTTT22540.1001951894751488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11100.011.8185881
GGCGAGG8300.011.20529519
TAGGACC6800.011.19384
TAATACG600.005809651711.1005184
GTCTTAT2450.010.8642931
ACCGCTT750.002638314910.1379758
CTAGTAC1451.6002377E-79.8349343
TGGCGAG17950.09.83385418
GTATCAA21700.09.812911
TGTAGGA20250.09.6240452
TACGGCT800.0044594089.514734
GTAGGAC19100.09.5071033
GACCGTC800.0044947849.5056227
TAGGACA6650.09.4431914
TTAGGAC12650.09.3943713
AGGACGT19250.09.2923085
CTAATAC1856.6138455E-99.25015453
GGACGTG18800.09.2094276
GACGTGA10650.09.1932317
GTATAAA3350.09.0806031
Produced by FastQC (version 0.11.5)