##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512955_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2249609
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.424095031625495	31.0	31.0	34.0	27.0	34.0
2	30.590563071182594	31.0	31.0	34.0	27.0	34.0
3	30.69080360187037	31.0	31.0	34.0	27.0	34.0
4	34.16305144582903	37.0	35.0	37.0	30.0	37.0
5	33.80840003751763	37.0	35.0	37.0	29.0	37.0
6	33.66112022133624	37.0	35.0	37.0	28.0	37.0
7	33.541800375087405	37.0	35.0	37.0	28.0	37.0
8	33.42837488648027	37.0	35.0	37.0	27.0	37.0
9	34.89084147511856	39.0	35.0	39.0	27.0	39.0
10	34.77915584441563	39.0	35.0	39.0	27.0	39.0
11	34.79926022699945	39.0	35.0	39.0	27.0	39.0
12	34.6522191189669	39.0	35.0	39.0	27.0	39.0
13	34.635215275187825	39.0	35.0	39.0	27.0	39.0
14	35.68671177969149	40.0	36.0	41.0	27.0	41.0
15	35.69286040374127	40.0	36.0	41.0	27.0	41.0
16	35.67070188641671	40.0	36.0	41.0	27.0	41.0
17	35.64852958891967	40.0	36.0	41.0	27.0	41.0
18	35.56906022335437	40.0	36.0	41.0	27.0	41.0
19	35.553161015981	40.0	36.0	41.0	26.0	41.0
20	35.52709470845823	40.0	36.0	41.0	26.0	41.0
21	35.4727510425145	40.0	36.0	41.0	26.0	41.0
22	35.42949463662352	40.0	36.0	41.0	26.0	41.0
23	35.32517473036425	40.0	36.0	41.0	25.0	41.0
24	35.29959295148623	40.0	36.0	41.0	25.0	41.0
25	35.20574730986585	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6332458071441245
1101	2	0.6125457561763916
1101	3	0.5938058750540129
1101	4	0.39608164776820587
1101	5	0.7414175250082238
1101	6	0.6711262322428624
1101	7	0.7454929633068588
1101	8	0.7180405649536326
1101	9	0.575698190967266
1101	10	0.952491291051885
1101	11	0.8857667629533523
1101	12	0.9233213830995126
1101	13	1.0051684216019794
1101	14	1.0511465717970339
1101	15	1.0587533998771619
1101	16	1.177828506920278
1101	17	1.0596320627995794
1101	18	0.6343739940150215
1101	19	1.0714003854424092
1101	20	0.9801680067191256
1101	21	1.192724565589124
1101	22	1.061090777492666
1101	23	1.0671567296452054
1101	24	0.9172569790036462
1101	25	1.065758141737284
1102	1	0.598012656879856
1102	2	0.5080427383278128
1102	3	0.5070008321293891
1102	4	0.5408533374457747
1102	5	0.5741560299981288
1102	6	0.553259503099099
1102	7	0.6476988637837948
1102	8	0.6809979845615928
1102	9	0.8788464316772107
1102	10	1.049551352493907
1102	11	0.8685981831374647
1102	12	0.8516861781723648
1102	13	0.969299912504809
1102	14	1.0255689953813913
1102	15	1.0011349680505077
1102	16	0.9968985384391971
1102	17	0.9426561573920651
1102	18	1.0549330911532664
1102	19	1.0549908311566654
1102	20	1.045565412615332
1102	21	1.0702471862185803
1102	22	1.1121608035905766
1102	23	1.1855888527643828
1102	24	1.2092299007403255
1102	25	1.1268881148431689
1103	1	0.7256907027782233
1103	2	0.6169483680024364
1103	3	0.676858336974643
1103	4	0.6152257495856333
1103	5	0.7891179506916117
1103	6	0.8151443297876426
1103	7	0.7765173257916871
1103	8	0.8605762937293235
1103	9	0.8263432145815415
1103	10	0.9556978209682754
1103	11	0.9450170192357064
1103	12	0.9838502702632681
1103	13	1.035804042829831
1103	14	1.0669672871394908
1103	15	0.9112846382229733
1103	16	1.1213427630106096
1103	17	1.2094537628751567
1103	18	1.1445986501634309
1103	19	1.2172111422174225
1103	20	1.1112311847842804
1103	21	0.7744846384664044
1103	22	1.0912592066677007
1103	23	1.056179472765102
1103	24	1.0526378781083068
1103	25	1.1040392028369865
1104	1	0.4663014355755486
1104	2	0.6226905762664643
1104	3	0.5149068961510146
1104	4	0.5169319980674558
1104	5	0.5346402440067237
1104	6	0.7073424461652493
1104	7	0.6259600367415246
1104	8	0.583684062752333
1104	9	0.926279099697588
1104	10	0.8069146787388277
1104	11	0.894696171657003
1104	12	0.81850635869435
1104	13	0.8893727581585225
1104	14	1.0673691976863893
1104	15	1.044579424891161
1104	16	1.076844603653278
1104	17	0.8493085630072201
1104	18	0.9016519193334958
1104	19	0.9289415567022559
1104	20	1.0042341007354025
1104	21	0.8609173403025139
1104	22	1.0370166123526516
1104	23	0.8146269940028006
1104	24	0.7972745657326499
1104	25	1.0169848402158266
1105	1	0.4162809424910954
1105	2	0.4887469038960397
1105	3	0.44993800250454896
1105	4	0.37192819913264685
1105	5	0.30267516803530015
1105	6	0.5106720029120311
1105	7	0.5566424411061845
1105	8	0.5765502560774891
1105	9	0.6604150532593067
1105	10	0.6336542524277462
1105	11	0.6375656364441937
1105	12	0.5586873278968909
1105	13	0.6664845757618281
1105	14	0.86789951380009
1105	15	0.6715880016145377
1105	16	0.8014068860707653
1105	17	0.5746377407490684
1105	18	0.8877048284871805
1105	19	0.847989188058726
1105	20	0.9228054718398795
1105	21	0.9740553218475867
1105	22	0.962127838023271
1105	23	1.032356525030238
1105	24	0.9553422415390003
1105	25	0.9641201945749387
1106	1	0.41831058144356703
1106	2	0.44052317771339844
1106	3	0.35746837230716366
1106	4	0.4270627168897576
1106	5	0.3837368941153372
1106	6	0.5517359869161496
1106	7	0.5194744214809006
1106	8	0.5368555808060478
1106	9	0.535786971470344
1106	10	0.6352360054420458
1106	11	0.45484594437100156
1106	12	0.6419833374020385
1106	13	0.4701976386406699
1106	14	0.6783458220861718
1106	15	0.529435598493393
1106	16	0.6535557977722632
1106	17	0.4617302791301583
1106	18	0.6752113356989469
1106	19	0.7679169119731455
1106	20	0.7752410466238828
1106	21	0.7391668888678069
1106	22	0.7275387729694884
1106	23	0.8088322529092125
1106	24	0.6771261109229201
1106	25	0.7752418698259547
1107	1	0.6091463026266979
1107	2	0.5208848642555779
1107	3	0.5325497844603397
1107	4	0.5053885160099938
1107	5	0.5847204790608131
1107	6	0.6157410177610956
1107	7	0.6531477446374367
1107	8	0.5952000406450253
1107	9	0.48650912672559343
1107	10	0.726312372506662
1107	11	0.5862722580734996
1107	12	0.4748878455933436
1107	13	0.5519633500012446
1107	14	0.7549537620629465
1107	15	0.8649519505199095
1107	16	0.6066441340633872
1107	17	0.6221389474462882
1107	18	0.751651438408075
1107	19	0.9237217272805367
1107	20	0.9043280838972905
1107	21	0.8650026626731844
1107	22	0.8330318153194796
1107	23	0.8062621507801211
1107	24	0.847529603267489
1107	25	0.7824541244628662
1108	1	0.4966398000729235
1108	2	0.4447220255537623
1108	3	0.5097772682004234
1108	4	0.4610123734520357
1108	5	0.5386541434370571
1108	6	0.5655033154755529
1108	7	0.497658145113121
1108	8	0.592834398239809
1108	9	0.5288353755063184
1108	10	0.7746456153196348
1108	11	0.7046155188220595
1108	12	0.7104170145504938
1108	13	0.678261623908746
1108	14	0.6905440244160701
1108	15	0.7249778288787567
1108	16	0.6607646889772809
1108	17	0.6610442329040822
1108	18	0.6990275304211337
1108	19	0.7848712092581422
1108	20	0.8435614245664311
1108	21	0.9798075452473469
1108	22	0.9725696340466428
1108	23	0.8799090466485282
1108	24	0.9201766677235312
1108	25	0.8521360714899302
1109	1	0.08309706757067659
1109	2	0.09269075932451543
1109	3	0.10523009592898092
1109	4	0.230754871258668
1109	5	0.20261179214943326
1109	6	0.04499949338455167
1109	7	0.08614303243933819
1109	8	0.2694732343970756
1109	9	0.3876561294935996
1109	10	0.281540075221379
1109	11	0.24864138837814664
1109	12	0.2785991843136557
1109	13	0.3015536166336261
1109	14	0.45151803331329177
1109	15	0.4437613232798725
1109	16	0.23042199360202176
1109	17	0.33851345396657706
1109	18	0.2934897526040672
1109	19	0.44121064276215805
1109	20	0.42546897836189146
1109	21	0.47006834025111033
1109	22	0.4921198670848028
1109	23	0.49104471264705296
1109	24	0.13077675479516415
1109	25	0.27188542011015215
1110	1	0.3352370696731235
1110	2	0.34901381588096214
1110	3	0.3015478800922651
1110	4	0.3883692922997781
1110	5	0.4190860608798914
1110	6	0.3284388103935072
1110	7	0.4114904382956297
1110	8	0.46550896179405044
1110	9	0.4554372134700557
1110	10	0.5136287927260739
1110	11	0.460121989635482
1110	12	0.47123148353954747
1110	13	0.12922522137239412
1110	14	0.5052388857367092
1110	15	0.5904292924223498
1110	16	0.6173973697596935
1110	17	0.5076248062749968
1110	18	0.4899512377565003
1110	19	0.6409102418480188
1110	20	0.5646510168382761
1110	21	0.613084909866032
1110	22	0.4847951449138179
1110	23	0.5998695104644156
1110	24	0.49687566956994544
1110	25	0.5484802318924551
1111	1	-0.42919968878580406
1111	2	-0.17309287455646682
1111	3	-0.20637309822615535
1111	4	-0.07996196135582778
1111	5	0.028043023629038544
1111	6	-0.15852155085661224
1111	7	0.019413119428563164
1111	8	0.1278064704180153
1111	9	-0.21531148239182585
1111	10	-0.20213701246164817
1111	11	-0.05123019953057906
1111	12	-0.17737753480372476
1111	13	-0.03926431483240833
1111	14	0.09538608415142846
1111	15	0.26620279591569584
1111	16	-0.0636426618255328
1111	17	0.19306221774349552
1111	18	0.16978417185243444
1111	19	0.2233153366690459
1111	20	0.1153015513834319
1111	21	0.030156219381872518
1111	22	-0.01255769132175999
1111	23	0.03929323051220024
1111	24	0.2003924567470392
1111	25	0.15239219083799327
1112	1	0.18805696447666875
1112	2	0.041974516879669466
1112	3	0.13405933857542962
1112	4	0.005738525640758496
1112	5	0.24879150302766817
1112	6	0.16137175445589236
1112	7	0.06898088470025243
1112	8	0.29061367230941926
1112	9	0.017452273746151548
1112	10	0.337004176133334
1112	11	0.1446483140184327
1112	12	0.30089595116228907
1112	13	0.19428833535855006
1112	14	0.29146545853257777
1112	15	0.2837241651641804
1112	16	0.08104099246750707
1112	17	0.37452765311035563
1112	18	0.3417473136876623
1112	19	0.3843390398638249
1112	20	0.5830777350276222
1112	21	0.6633972725877655
1112	22	0.6222700412140583
1112	23	0.8306994584104359
1112	24	0.7273312487530177
1112	25	0.6928040161658586
1113	1	-0.24176395993407596
1113	2	-0.6076925256937713
1113	3	-0.11009563099212016
1113	4	-0.18481165791371978
1113	5	-0.18671121229893117
1113	6	-0.2509210369318424
1113	7	-0.03269134058788126
1113	8	0.16457197065142992
1113	9	-0.010722658870562896
1113	10	-0.3110690217135996
1113	11	-0.05527666458687719
1113	12	-0.06997255344856512
1113	13	-0.04782090155070051
1113	14	0.08886018464050949
1113	15	0.19983504966482712
1113	16	-0.17023333150014253
1113	17	-0.03640755495803916
1113	18	0.11594395292112836
1113	19	-0.025474846292411257
1113	20	0.22230544655516837
1113	21	-0.06120702554629531
1113	22	-0.18449836022238486
1113	23	9.767496422128374E-4
1113	24	-0.34645413247243795
1113	25	-0.3982604398490466
1114	1	-0.5403374337580615
1114	2	-0.3671627519994587
1114	3	-0.4351164554551765
1114	4	-0.1834129999472296
1114	5	-0.2708654549270548
1114	6	-0.6370374210978582
1114	7	-0.457908469623014
1114	8	-0.8769532761541754
1114	9	-0.7009528170202302
1114	10	-0.9074914927906406
1114	11	-0.6899660139739723
1114	12	-0.767461443704498
1114	13	-0.6887226334969512
1114	14	-1.7149828188485117
1114	15	-1.0641686852565115
1114	16	-1.1254520573869158
1114	17	-1.2118218685290003
1114	18	-1.3166793223297546
1114	19	-1.28373266016861
1114	20	-0.6310365669342772
1114	21	-0.6852953952189011
1114	22	-0.818776144916086
1114	23	-0.6661561363466149
1114	24	-1.2566524601538234
1114	25	-1.0613092445757317
1115	1	-1.4687624662933843
1115	2	-1.269924110921064
1115	3	-0.9838083354417648
1115	4	-2.334173152677174
1115	5	-1.4904818204463695
1115	6	-1.4133104069536273
1115	7	-1.183397992246718
1115	8	-1.0132906898094731
1115	9	-1.4208171321010425
1115	10	-1.3847535242073477
1115	11	-1.175420069373466
1115	12	-1.7788376007781963
1115	13	-1.2596543791325487
1115	14	-1.9898138052336947
1115	15	-2.0164046098256208
1115	16	-1.4418296269789153
1115	17	-1.5783210094999944
1115	18	-1.4165634517531842
1115	19	-1.3695024177734894
1115	20	-1.3909731570460835
1115	21	-1.5514460429356802
1115	22	-1.8090530126399216
1115	23	-2.050240277695579
1115	24	-1.6531083823062218
1115	25	-1.3650397648589845
1116	1	-0.9180695014317521
1116	2	-0.9642572390674502
1116	3	-0.7222468668580078
1116	4	-0.8994505446246279
1116	5	-0.8414314525580053
1116	6	-1.6189995107924986
1116	7	-0.849328188708121
1116	8	-1.028243066449491
1116	9	-1.0372785098396022
1116	10	-1.0633745537073551
1116	11	-1.0304089472619395
1116	12	-1.5288115546817025
1116	13	-1.1535817021608494
1116	14	-1.4723505196236673
1116	15	-1.3671623533892543
1116	16	-1.7589212916977317
1116	17	-1.514414535586674
1116	18	-2.3556447934881675
1116	19	-1.6093274500098644
1116	20	-1.508749322155083
1116	21	-1.5014366402336563
1116	22	-2.8290415662269623
1116	23	-2.630917215131511
1116	24	-1.8334525334380345
1116	25	-2.2808609022147195
1201	1	0.6801876294534672
1201	2	0.7006167622240156
1201	3	0.6001183340577576
1201	4	0.5881212399235523
1201	5	0.6773395109591505
1201	6	0.7723711616391924
1201	7	0.7840502851377096
1201	8	0.7507890642839072
1201	9	1.0054434428215586
1201	10	0.8116263065938938
1201	11	0.9660534940098486
1201	12	0.7297796159959944
1201	13	0.8152875381222984
1201	14	0.8725513351858254
1201	15	0.9467965910109939
1201	16	0.9497766575465221
1201	17	0.9111236587523379
1201	18	0.9746797121422475
1201	19	0.854842116378066
1201	20	1.0825262110798732
1201	21	1.0679803589141414
1201	22	1.1325331291757905
1201	23	1.1095637033868258
1201	24	1.2121584120229159
1201	25	1.0921555973510877
1202	1	0.5313269081866849
1202	2	0.6370358913802256
1202	3	0.5170565601081485
1202	4	0.6306901226589119
1202	5	0.6698617333922314
1202	6	0.7543725791882565
1202	7	0.506253823069521
1202	8	0.5290871967991677
1202	9	0.6352604073688042
1202	10	0.647119078166341
1202	11	0.698571557858962
1202	12	0.7135252459760864
1202	13	0.766380032785861
1202	14	0.7145302600763515
1202	15	0.8287716873638971
1202	16	0.6432961150163337
1202	17	0.8920899684463777
1202	18	0.8827919212992228
1202	19	1.0069770624278647
1202	20	0.914186319450593
1202	21	0.8784362271794421
1202	22	0.7822429771811983
1202	23	0.7696961674880995
1202	24	0.7456055563381554
1202	25	0.8496775598726742
1203	1	0.6303571785869835
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2211	9	-0.23049863191437936
2211	10	-0.5017481224215175
2211	11	-0.1656804042555251
2211	12	-0.19721039863288325
2211	13	-0.1934758139667423
2211	14	-1.3401943274275894
2211	15	-0.5727783617503519
2211	16	-0.2720436781161695
2211	17	-0.11443704986250225
2211	18	-0.3188436691963332
2211	19	-0.1350395824791235
2211	20	-0.30214458159655777
2211	21	-0.33046394057053874
2211	22	-0.1483162747559632
2211	23	-0.2063437125382208
2211	24	-0.24328179227992308
2211	25	-0.3739906116976144
2212	1	-0.14178392546892837
2212	2	-0.06810728190633952
2212	3	-0.06551757511465084
2212	4	0.10498887401383428
2212	5	0.10061949726475206
2212	6	0.1583240214395687
2212	7	0.0639751768927539
2212	8	-0.07749172924467729
2212	9	-0.20243813634997565
2212	10	-0.06866552682448912
2212	11	0.005502359476196261
2212	12	0.035160588736196985
2212	13	0.06061274351795731
2212	14	-0.04599240210244204
2212	15	0.019458504121345754
2212	16	-0.01259003911044232
2212	17	-0.1818407642130353
2212	18	0.05758840832704237
2212	19	0.06554767794159488
2212	20	-0.058442667431847894
2212	21	-0.037537233273560844
2212	22	-0.09169353903771338
2212	23	-0.4515019445292765
2212	24	-0.255655863411306
2212	25	-0.3698765276897831
2213	1	-0.3623285650082799
2213	2	-0.507433870021579
2213	3	-0.39854509892158063
2213	4	-0.3571893929466441
2213	5	-0.3854821798396344
2213	6	-0.19940839643814456
2213	7	-0.7906252914278653
2213	8	-0.547747229226637
2213	9	-0.7949585217174615
2213	10	-0.5261778378076158
2213	11	-0.6072571618720204
2213	12	-0.8952452448245012
2213	13	-0.6256513708287486
2213	14	-0.5857627806647798
2213	15	-0.7327803887732571
2213	16	-0.8367088360619874
2213	17	-0.8150416767988844
2213	18	-0.8868668856647375
2213	19	-1.3039047832105197
2213	20	-0.7886210192192635
2213	21	-1.4774477978347704
2213	22	-0.9428477550311385
2213	23	-1.0203673273551175
2213	24	-1.082202989519196
2213	25	-1.0741139810587086
2214	1	-1.096196105812286
2214	2	-1.0305380690091077
2214	3	-0.8593866485506467
2214	4	-1.5444427278553192
2214	5	-1.4342885540784565
2214	6	-2.720378999027666
2214	7	-1.5198471874671924
2214	8	-2.068241032684025
2214	9	-1.471590530798963
2214	10	-1.6556119354740702
2214	11	-1.4517177662807939
2214	12	-1.5688538338548668
2214	13	-1.7869202439179404
2214	14	-1.822233164742336
2214	15	-2.2215738469750406
2214	16	-1.6335190649725675
2214	17	-1.7750534851716182
2214	18	-1.66042455570296
2214	19	-3.2838817370799944
2214	20	-2.0982737765313573
2214	21	-2.5942231386765044
2214	22	-2.213291210111322
2214	23	-2.1708869897390386
2214	24	-1.6677222256201887
2214	25	-1.5158959846690223
2215	1	-1.6605625891021134
2215	2	-1.4738207223922188
2215	3	-1.412962323561132
2215	4	-1.3329551569224591
2215	5	-2.080043848261351
2215	6	-2.01367395841784
2215	7	-2.0849627772715813
2215	8	-2.881540186516048
2215	9	-2.3744668432952807
2215	10	-1.9076783047166757
2215	11	-2.3963672530682985
2215	12	-3.1378660947976833
2215	13	-2.5784783230715647
2215	14	-2.6508366582617526
2215	15	-3.548183420701889
2215	16	-3.200083297129723
2215	17	-2.8871138920671413
2215	18	-3.5557916040291673
2215	19	-4.244998741208573
2215	20	-3.6307819140951807
2215	21	-2.8850501370160586
2215	22	-3.7805806300650815
2215	23	-3.766406216575149
2215	24	-2.7787399150720375
2215	25	-2.956514820832737
2216	1	-1.22863074800512
2216	2	-1.0420249974476725
2216	3	-1.4177465542528047
2216	4	-0.9783977864848694
2216	5	-0.9506629246880536
2216	6	-1.3454495180064683
2216	7	-2.550192662329053
2216	8	-1.775420000994771
2216	9	-1.7387489566245833
2216	10	-1.7070430960052434
2216	11	-2.1834664666194215
2216	12	-1.6832986416821356
2216	13	-2.1636920758443026
2216	14	-2.1437182876225123
2216	15	-2.2355699905748523
2216	16	-1.9958183243529533
2216	17	-2.2175081247753496
2216	18	-2.092648833309262
2216	19	-2.1764113243906635
2216	20	-2.611347552328695
2216	21	-2.432045498984195
2216	22	-2.7484471354089024
2216	23	-1.8782684871942266
2216	24	-2.180479177020011
2216	25	-2.1594438387799073
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58957.0
3	0.0
4	974.0
5	8512.0
6	9750.0
7	7424.0
8	6113.0
9	5424.0
10	4789.0
11	4228.0
12	3988.0
13	3761.0
14	3575.0
15	3655.0
16	3704.0
17	3975.0
18	4396.0
19	5195.0
20	6305.0
21	8280.0
22	10589.0
23	12944.0
24	15316.0
25	18144.0
26	20071.0
27	22242.0
28	25403.0
29	30415.0
30	38521.0
31	49206.0
32	62710.0
33	80611.0
34	105568.0
35	137705.0
36	189511.0
37	294652.0
38	982996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.374138619816485	19.433493219731247	13.466136025332695	27.726232135119577
2	13.582822947662834	20.738075911959694	40.091420453181584	25.587680687195892
3	16.634179539644446	25.02754923188874	30.361098306416807	27.97717292205001
4	11.325450348802724	17.545428392172628	38.030724164165164	33.098397094859486
5	12.553125236322227	38.172545251535816	36.30914728268364	12.965182229458316
6	31.433926572623918	37.844363218333534	18.145390190820066	12.57632001822249
7	27.23519987050762	31.616195618870847	23.010333153828675	18.138271356792856
8	24.61333711719029	37.22683614550116	20.761676171531487	17.39815056577706
9	26.08138001086917	14.90797229518243	20.09012871337326	38.92051898057514
10	14.137810118155505	29.182478880459662	35.22664568655861	21.45306531482622
11	33.86242398378321	22.378907500266724	25.075438315729574	18.683230200220493
12	22.69340031564674	24.847222530953385	32.243225820792674	20.2161513326072
13	28.597072410886703	21.914197669166782	25.940255061578238	23.548474858368273
14	20.537682209587146	21.989490857846512	27.91617581030198	29.556651122264356
15	22.598784160748618	30.923126507296146	23.477744807121663	23.000344524833572
16	22.080660055031263	28.073545737344656	27.625755412763358	22.220038794860724
17	21.56938071354889	28.589220289545764	27.820047664781416	22.021351332123928
18	21.43791599265466	27.690124729450577	29.4975215472693	21.374437730625463
19	23.240028912397747	27.05607422626885	27.54301519323386	22.160881668099545
20	23.301898367290327	27.517968341578086	27.916899309716953	21.263233981414636
21	22.78539194384088	26.79226780843596	27.228077069427208	23.19426317829595
22	22.86712176959381	27.998876245165256	27.328490696794677	21.80551128844626
23	22.114154059661033	27.727129470054575	27.690500882597817	22.468215587686572
24	22.842201841387478	26.959230230333464	28.258567714908555	21.94000021337051
25	22.617684909615054	27.33815060378877	27.69466714380019	22.34949734279599
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	147.0
1	147.0
2	271.5
3	396.0
4	396.0
5	396.0
6	916.5
7	1437.0
8	1437.0
9	1437.0
10	2362.0
11	3287.0
12	3287.0
13	3287.0
14	6218.0
15	9149.0
16	9149.0
17	9149.0
18	17148.5
19	25148.0
20	25148.0
21	25148.0
22	41116.0
23	57084.0
24	57084.0
25	57084.0
26	82744.0
27	108404.0
28	108404.0
29	108404.0
30	139005.5
31	169607.0
32	169607.0
33	169607.0
34	195128.5
35	220650.0
36	220650.0
37	220650.0
38	239999.0
39	259348.0
40	259348.0
41	259348.0
42	272132.0
43	284916.0
44	284916.0
45	284916.0
46	289551.5
47	294187.0
48	294187.0
49	294187.0
50	283423.5
51	272660.0
52	272660.0
53	272660.0
54	242548.5
55	212437.0
56	212437.0
57	212437.0
58	181716.5
59	150996.0
60	150996.0
61	150996.0
62	122075.5
63	93155.0
64	93155.0
65	93155.0
66	71854.5
67	50554.0
68	50554.0
69	50554.0
70	36680.0
71	22806.0
72	22806.0
73	22806.0
74	15812.5
75	8819.0
76	8819.0
77	8819.0
78	5952.5
79	3086.0
80	3086.0
81	3086.0
82	2049.0
83	1012.0
84	1012.0
85	1012.0
86	636.5
87	261.0
88	261.0
89	261.0
90	160.0
91	59.0
92	59.0
93	59.0
94	31.0
95	3.0
96	3.0
97	3.0
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07836917437652499
2	1.3335650773089902E-4
3	0.0
4	0.02800486662348879
5	0.07196806200544184
6	0.08165863490055383
7	0.03680639613372813
8	0.06498907143419144
9	0.04654142119808376
10	0.10672965835396284
11	0.003956243062683337
12	0.011513111834100948
13	0.005023095124530529
14	0.006178851524864987
15	0.005956590678646823
16	0.015202641881322488
17	0.02529328429962718
18	8.890433848726601E-4
19	0.0024448693083998154
20	0.004311860416632401
21	4.4452169243633004E-5
22	3.5561735394906404E-4
23	0.0
24	4.4452169243633004E-5
25	0.0028449388315925123
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2249609.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.55962173926616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.45986568046416	59.832566624451744
2	12.15265928081491	17.635847210842243
3	3.009127249959873	6.550234050672198
4	1.0472375795115625	3.039486505620146
5	0.4573630363186065	1.659304445640017
6	0.23294338669818918	1.0141370415290525
7	0.1488892258038169	0.7562342133763997
8	0.09195766006192806	0.5337930424097218
9	0.0649089052688203	0.42387890524339156
>10	0.3034183852174353	3.7356826766938402
>50	0.01722110428020872	0.8711089465365172
>100	0.011652338706760633	1.6741917490756448
>500	0.0017761964426030899	0.856652971511122
>1k	9.799704510899472E-4	1.4168816163979443
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3265	0.14513633258046177	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3215	0.14291372411828013	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2954	0.1313117079456919	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2938	0.13060047323779378	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2893	0.1286001256218303	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2254	0.1001951894751488	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.4452169243633004E-5	0.0	0.0	0.0
6	0.0	4.4452169243633004E-5	0.0	0.0	0.0
7	0.0	4.4452169243633004E-5	0.0	0.0	0.0
8	0.0	4.4452169243633004E-5	0.0	0.0	0.0
9	0.0	4.4452169243633004E-5	0.0	0.0	0.0
10	0.0	4.4452169243633004E-5	0.0	4.4452169243633004E-5	0.0
11	0.0	4.4452169243633004E-5	0.0	8.890433848726601E-5	0.0
12	0.0	4.4452169243633004E-5	0.0	8.890433848726601E-5	0.0
13	0.0	4.4452169243633004E-5	0.0	8.890433848726601E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1110	0.0	11.818588	1
GGCGAGG	830	0.0	11.205295	19
TAGGACC	680	0.0	11.1938	4
TAATACG	60	0.0058096517	11.100518	4
GTCTTAT	245	0.0	10.864293	1
ACCGCTT	75	0.0026383149	10.137975	8
CTAGTAC	145	1.6002377E-7	9.834934	3
TGGCGAG	1795	0.0	9.833854	18
GTATCAA	2170	0.0	9.81291	1
TGTAGGA	2025	0.0	9.624045	2
TACGGCT	80	0.004459408	9.51473	4
GTAGGAC	1910	0.0	9.507103	3
GACCGTC	80	0.004494784	9.505622	7
TAGGACA	665	0.0	9.443191	4
TTAGGAC	1265	0.0	9.394371	3
AGGACGT	1925	0.0	9.292308	5
CTAATAC	185	6.6138455E-9	9.2501545	3
GGACGTG	1880	0.0	9.209427	6
GACGTGA	1065	0.0	9.193231	7
GTATAAA	335	0.0	9.080603	1
>>END_MODULE