FastQCFastQC Report
Thu 26 May 2016
SRR1512954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144890
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33560.15646490029791738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC456.7865744E-414.7712310
GTGCGCC751.485957E-512.66105511
ACCGAGT701.09603534E-412.208598
GGTATCA9450.011.1911021
TAAGGCG700.001495580910.853855
ACCGTCC803.7882262E-410.6825158
GTATCAA20100.010.6177781
TAAGACA2950.010.3019614
GTAGGAC6750.010.2709593
TTAGGAC4200.010.1754863
GTCTAAT951.5971018E-410.0290111
TAGGACG5500.010.0151444
GTAAGAC1901.0004442E-109.9969683
TAGGACA3550.09.6308824
GTCCTAC7150.09.5941861
TAGGACC3500.09.497124
GTCCTAA3950.09.4069581
GGACGTG5350.09.4066076
GTCCTAG2650.09.3477951
GTTCTAA1951.5970727E-99.2832631
Produced by FastQC (version 0.11.5)