##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512954_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2144890
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.581588333201235	31.0	30.0	33.0	16.0	34.0
2	28.612945186000214	31.0	30.0	34.0	16.0	34.0
3	28.800509583242032	31.0	30.0	34.0	18.0	34.0
4	32.270670290784146	35.0	33.0	37.0	22.0	37.0
5	32.350982567870616	35.0	33.0	37.0	23.0	37.0
6	32.46258129787542	35.0	33.0	37.0	23.0	37.0
7	32.21693233685644	35.0	33.0	37.0	22.0	37.0
8	32.23477287879565	35.0	33.0	37.0	22.0	37.0
9	33.5487339677093	38.0	34.0	39.0	19.0	39.0
10	33.42173304924728	38.0	33.0	39.0	18.0	39.0
11	33.48277394178722	38.0	34.0	39.0	18.0	39.0
12	33.38902228086289	38.0	34.0	39.0	17.0	39.0
13	33.3741842238996	38.0	34.0	39.0	17.0	39.0
14	34.32063089482444	39.0	34.0	41.0	17.0	41.0
15	34.25881513737301	39.0	34.0	41.0	17.0	41.0
16	34.13353738420152	39.0	33.0	41.0	17.0	41.0
17	34.04806773307722	39.0	33.0	41.0	16.0	41.0
18	33.74016942593793	39.0	33.0	41.0	10.0	41.0
19	33.9809850388598	39.0	34.0	41.0	13.0	41.0
20	34.07987728974446	39.0	34.0	41.0	11.0	41.0
21	34.00830392234567	39.0	34.0	41.0	10.0	41.0
22	33.896961615747195	39.0	34.0	41.0	8.0	41.0
23	33.617744499717936	39.0	33.0	41.0	2.0	41.0
24	33.627279720638356	39.0	34.0	41.0	2.0	41.0
25	33.52146403778282	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.688288159145312
1101	2	1.0507310497212927
1101	3	0.9853348847171084
1101	4	1.0170479830072807
1101	5	0.8238365489596617
1101	6	1.0201146970234802
1101	7	0.9907439234915998
1101	8	0.8353710414306335
1101	9	0.7613009790797847
1101	10	0.9477251356572367
1101	11	1.0980655790734914
1101	12	1.2655175051798935
1101	13	1.136753535671808
1101	14	1.1091522699451914
1101	15	0.8162148036160417
1101	16	1.344951485240955
1101	17	1.3195747172468728
1101	18	1.570696862599661
1101	19	1.0019477372058674
1101	20	1.2167746819811214
1101	21	1.1345142905071057
1101	22	1.2460201948715692
1101	23	1.4779671563121042
1101	24	1.0867223386218896
1101	25	1.38260985993859
1102	1	0.5438948482819477
1102	2	0.560213808341981
1102	3	0.5893065595939575
1102	4	0.7490504028499885
1102	5	0.5162442924563351
1102	6	0.5729126401571776
1102	7	0.8016937117553624
1102	8	0.6433748872520866
1102	9	0.6711688599889527
1102	10	0.8588110400734479
1102	11	0.8922185260962223
1102	12	0.7595921314006162
1102	13	0.7853421408053336
1102	14	0.794250251969217
1102	15	1.0104347284280735
1102	16	0.9340713754838035
1102	17	0.9127235375735481
1102	18	1.20736288109417
1102	19	1.1876976723886514
1102	20	1.0798704602198228
1102	21	0.9979136293715101
1102	22	1.2297111031768182
1102	23	1.385112174028805
1102	24	1.2650836513865542
1102	25	1.0602020084132207
1103	1	0.38919715134287003
1103	2	0.5588359716193168
1103	3	0.5817097741954456
1103	4	0.6099404706366087
1103	5	0.1758228365176393
1103	6	0.6538808099184479
1103	7	0.5158110120795598
1103	8	0.5160681422729283
1103	9	0.8233327162476414
1103	10	0.758744727076099
1103	11	0.7807171682586542
1103	12	0.914314564140291
1103	13	0.9135075338414111
1103	14	1.1439452648107817
1103	15	1.1326635293206664
1103	16	1.128522924892252
1103	17	0.9416139000846044
1103	18	1.2767868000870592
1103	19	1.2498187409752362
1103	20	1.2855773251939269
1103	21	0.9719349863670033
1103	22	1.1743013309566948
1103	23	1.4723053448426668
1103	24	1.209172584484108
1103	25	1.227414178427118
1104	1	0.503701939001445
1104	2	0.4057004263468471
1104	3	0.5086657010607603
1104	4	0.6579555123390364
1104	5	0.42916400048552816
1104	6	0.5053092338311416
1104	7	0.6504528358429553
1104	8	0.6016065521808329
1104	9	0.6556322767425158
1104	10	0.8915725972510629
1104	11	0.824100260541826
1104	12	0.6419478839206008
1104	13	0.6325992662832576
1104	14	0.8850840197829157
1104	15	0.9931106929744757
1104	16	0.7397808819141147
1104	17	0.831835459714668
1104	18	1.0934951369162107
1104	19	0.9344660074599034
1104	20	0.8968191046410894
1104	21	0.9759232748355373
1104	22	0.8650630322696173
1104	23	1.1146411973169563
1104	24	1.0453543326517618
1104	25	0.7198331345863167
1105	1	0.6307415381774177
1105	2	0.7370721126393107
1105	3	0.8567354213825737
1105	4	0.982790240248363
1105	5	0.7214127012832776
1105	6	0.5483191442222193
1105	7	0.9158052100132039
1105	8	0.8477448059512724
1105	9	0.7295094523234482
1105	10	0.9721385662058495
1105	11	0.8800742171531297
1105	12	0.9438981708895824
1105	13	0.9887320943477249
1105	14	1.0618678477880366
1105	15	1.2216644032248212
1105	16	1.231233106029677
1105	17	1.150150947561933
1105	18	1.2311869229060193
1105	19	1.2237749081610119
1105	20	1.196805709929457
1105	21	1.2868726886049586
1105	22	0.9835874097970247
1105	23	1.1117962622982631
1105	24	1.2373470079486921
1105	25	1.247506015381866
1106	1	0.5223558202996195
1106	2	0.5357783640611764
1106	3	0.5031535159022518
1106	4	0.561126986060593
1106	5	0.643269329275185
1106	6	0.5566264349534435
1106	7	0.42378502250646477
1106	8	0.616969634429303
1106	9	0.7235651535579279
1106	10	0.8066632737504236
1106	11	0.6721860027776145
1106	12	0.6799836385189266
1106	13	0.6903832551892322
1106	14	0.8562133103884619
1106	15	0.9442436385900734
1106	16	0.908374885547623
1106	17	0.8247142101312335
1106	18	1.1604618010745327
1106	19	0.6579541770523747
1106	20	0.7546035235482407
1106	21	0.6585634820961062
1106	22	0.6803186142224718
1106	23	0.9055711823744161
1106	24	0.7571645301298062
1106	25	0.9283260879419402
1107	1	0.2611425603515478
1107	2	0.23727739160349515
1107	3	0.36776726806579774
1107	4	0.3503144074463691
1107	5	0.28531927091079723
1107	6	0.25892001351732574
1107	7	0.37997581459936924
1107	8	0.31172022452517467
1107	9	0.48714239318275787
1107	10	0.3344621939564334
1107	11	0.3112010810544277
1107	12	0.42929483724595485
1107	13	0.42936368291830007
1107	14	0.6617851523125822
1107	15	0.7565752566014226
1107	16	0.47821723190163823
1107	17	0.5967487219075522
1107	18	0.8824666758439008
1107	19	0.7098220277326277
1107	20	0.5433002525619202
1107	21	0.5920340073417165
1107	22	0.5454193356223342
1107	23	0.604810604966822
1107	24	0.6938140210121446
1107	25	0.7431340989454966
1108	1	0.4363825330110096
1108	2	0.3918230037442818
1108	3	0.2353348847171084
1108	4	0.38698143733275003
1108	5	0.41566957981265773
1108	6	0.5226252838353389
1108	7	0.5631879646289235
1108	8	0.5661786639351689
1108	9	0.5865732113846889
1108	10	0.475477706740115
1108	11	0.5234890515477773
1108	12	0.473487862106694
1108	13	0.4384927976197801
1108	14	0.7448147018871154
1108	15	0.6335771750618946
1108	16	0.6384330339402879
1108	17	0.8089879053896425
1108	18	0.8926871832288228
1108	19	0.898227229038902
1108	20	0.6822616753265507
1108	21	0.6574271761695414
1108	22	0.4650764562145824
1108	23	0.6608921411880857
1108	24	0.6967646858693186
1108	25	0.8561277062785777
1109	1	-0.06173123061999419
1109	2	-0.1187354990556031
1109	3	0.12370800584885089
1109	4	0.18760035864132618
1109	5	0.04172321965939574
1109	6	1.8201828862629554E-4
1109	7	-0.033260605957579514
1109	8	0.1559148855679453
1109	9	0.42928614978728064
1109	10	0.5954673913849717
1109	11	-0.019164941968284666
1109	12	0.3593484574500465
1109	13	0.37239347548597834
1109	14	0.14807140686795606
1109	15	0.46658631151930763
1109	16	0.28266380317106155
1109	17	0.15558383819247013
1109	18	-1.541368224964522
1109	19	-0.05315797579458348
1109	20	0.11286880765781859
1109	21	0.22451382515274076
1109	22	0.3375786666478291
1109	23	-0.12362745798668584
1109	24	0.2598651869008535
1109	25	0.29755859339911694
1110	1	0.2567316299857012
1110	2	0.1640121575117668
1110	3	0.29983822907142255
1110	4	0.11991133000230292
1110	5	0.2507533624246605
1110	6	0.17644224952467624
1110	7	0.3228569711852529
1110	8	0.18247258651030762
1110	9	0.5997695105860785
1110	10	0.6799479054156095
1110	11	0.5375463440935633
1110	12	-0.059235594046029405
1110	13	0.26442114139491224
1110	14	0.4275619044447794
1110	15	0.5120412542805681
1110	16	0.6076177296718939
1110	17	0.16937724714260582
1110	18	0.7032380336314361
1110	19	0.5135078337696442
1110	20	0.5662446838223048
1110	21	0.4470716751121202
1110	22	0.5552823466468482
1110	23	0.4721615892455162
1110	24	0.7819644700831674
1110	25	0.8170592438210633
1111	1	0.3911786572283802
1111	2	0.6516480813540042
1111	3	0.7171536525081166
1111	4	0.663873076387425
1111	5	0.5780357444114728
1111	6	0.7309933716919872
1111	7	0.6989353001154726
1111	8	0.508081905251764
1111	9	0.7828389728658749
1111	10	0.7180891041332629
1111	11	0.5614917726788775
1111	12	0.7024364637379747
1111	13	0.7497686805937249
1111	14	0.7216807323456678
1111	15	1.0402779168401466
1111	16	0.8184052825840809
1111	17	0.9237597101510957
1111	18	1.2177987635405145
1111	19	0.8542046586326819
1111	20	0.6989858306784029
1111	21	0.8295051830890898
1111	22	0.722452951355919
1111	23	1.078227331580159
1111	24	1.193611867007455
1111	25	0.5666051933610419
1112	1	-0.12252184313474501
1112	2	0.08293680606497844
1112	3	0.10950319854059387
1112	4	0.16692982072940765
1112	5	0.14738477359681212
1112	6	0.27886502110956
1112	7	-0.17543726537561355
1112	8	0.06038434176154084
1112	9	0.2858338684039623
1112	10	0.33377144003384984
1112	11	0.1653393804670742
1112	12	0.20483888914737491
1112	13	0.041392903106093115
1112	14	0.18761910158806927
1112	15	0.10115532539866479
1112	16	0.2735284836909102
1112	17	0.34326754311755536
1112	18	0.15234481357610719
1112	19	0.11318153537251163
1112	20	0.08258615668551528
1112	21	0.050239512947392484
1112	22	0.08161561758132763
1112	23	0.32493743885407156
1112	24	0.17512906768197212
1112	25	0.30946136392257984
1113	1	-0.19903692144086804
1113	2	-0.0017513657873706734
1113	3	-0.2842550964117301
1113	4	0.05472204464103214
1113	5	-0.2328601851556087
1113	6	-0.13213711108659965
1113	7	-0.2896495718267289
1113	8	0.11276898817603609
1113	9	-0.23986614356470426
1113	10	-0.4968357260957035
1113	11	-0.29541384774883994
1113	12	0.013389217399193853
1113	13	-0.10473159764527651
1113	14	0.021937707548467245
1113	15	-0.3763696425740619
1113	16	-0.3444156536562133
1113	17	-0.5884456166553065
1113	18	-0.6132572326229564
1113	19	-0.435800906147243
1113	20	-0.48394350566503874
1113	21	-0.3773875433363827
1113	22	-0.2586737582304366
1113	23	-0.6893832062128382
1113	24	0.00460479566516625
1113	25	-0.05670897239701134
1114	1	-1.3853577851297345
1114	2	-1.1963218406525336
1114	3	-1.062785479232545
1114	4	-1.292997129142698
1114	5	-1.2389964144297103
1114	6	-1.6959325761743287
1114	7	-1.8689339543019194
1114	8	-1.3212601349469217
1114	9	-1.5458161012716012
1114	10	-1.5905643407918788
1114	11	-2.2629411108847144
1114	12	-1.6881477049361138
1114	13	-1.816189481898597
1114	14	-2.008347793647566
1114	15	-1.9154635696480184
1114	16	-2.5072650265358725
1114	17	-4.609260009044366
1114	18	-3.459426750663468
1114	19	-2.4594741305189842
1114	20	-1.6734686405027333
1114	21	-2.6821654817976395
1114	22	-2.73552242167737
1114	23	-2.597631362142515
1114	24	-2.197290663055856
1114	25	-1.9922915712855662
1115	1	-0.9247559453688474
1115	2	-0.6001830170548423
1115	3	-0.7126180435806475
1115	4	-0.6642829304833455
1115	5	-0.379787453575414
1115	6	-0.5570079947634561
1115	7	-1.6503697803081288
1115	8	-1.046823525446328
1115	9	-0.7625811400110436
1115	10	-0.676030229767818
1115	11	-1.3553806802529849
1115	12	-0.8760824717739908
1115	13	-0.7494792877660998
1115	14	-0.7312060972371341
1115	15	-2.180577176333834
1115	16	-0.8835675134050831
1115	17	-0.9324351925851815
1115	18	-1.020772039540745
1115	19	-1.131389629198651
1115	20	-0.8646731905738321
1115	21	-1.6012728785649983
1115	22	-1.64739207142636
1115	23	-1.0656021116854788
1115	24	-0.9783092057562968
1115	25	-1.2035083090470948
1116	1	-0.805394655317901
1116	2	-1.5038666514281331
1116	3	-1.0788782010477007
1116	4	-0.7240928332242831
1116	5	-1.2531854157664775
1116	6	-1.003723080444061
1116	7	-0.6714495243189091
1116	8	-1.243768285666949
1116	9	-1.250274710808064
1116	10	-2.2244559802625368
1116	11	-0.8345569254637155
1116	12	-0.8332539592903032
1116	13	-1.4258308098072767
1116	14	-1.2409280036831731
1116	15	-0.8377535057515431
1116	16	-0.6453865947069275
1116	17	-0.9972676206934707
1116	18	-0.7591695806969128
1116	19	-1.2983687037639342
1116	20	-1.617053090367527
1116	21	-1.9330900652097682
1116	22	-1.0728987869330737
1116	23	-0.8530335882548883
1116	24	-1.5085182495860323
1116	25	-1.2498183591502467
1201	1	0.4200782298586532
1201	2	0.259429144059105
1201	3	0.4726281868145712
1201	4	0.6087047532671548
1201	5	0.63644691462558
1201	6	0.6837688181468451
1201	7	0.5040179072261637
1201	8	0.07942616712040973
1201	9	0.4586578633376135
1201	10	0.5647208533432106
1201	11	0.21980273916166482
1201	12	-0.4247081977894638
1201	13	0.06648075536915599
1201	14	0.5678799273196944
1201	15	0.3718418031769204
1201	16	0.8274049216555497
1201	17	0.9242352578754947
1201	18	1.1538971622679028
1201	19	0.7414854866800411
1201	20	0.6604691746758036
1201	21	0.5828052946192557
1201	22	0.9077099964170685
1201	23	1.0957129459227843
1201	24	0.836704132117589
1201	25	0.8561314737631278
1202	1	0.3104172674686012
1202	2	0.3694960467848034
1202	3	0.06632993463740533
1202	4	0.4570795999302817
1202	5	0.5383610807774986
1202	6	0.4623895258019459
1202	7	0.4644674343831028
1202	8	0.38671604123192793
1202	9	0.7464351964505482
1202	10	0.8031054439663947
1202	11	0.4343192386440151
1202	12	0.589977081000896
1202	13	0.5724532808818026
1202	14	0.7239161365712334
1202	15	0.37938806523725077
1202	16	0.5794889111111914
1202	17	0.7722734159190523
1202	18	1.142546231806719
1202	19	0.8950499490657435
1202	20	0.7838264039111635
1202	21	0.7677480923537843
1202	22	0.890464492123634
1202	23	0.9940572557111125
1202	24	1.0946973983451969
1202	25	0.9566214557542025
1203	1	0.2723510012495005
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2211	12	-0.14990570602464715
2211	13	-0.3159127355886824
2211	14	-0.13500996725649372
2211	15	-0.08573173682231783
2211	16	-0.0683215028607691
2211	17	-0.08851570255705354
2211	18	-0.018232357001068067
2211	19	-0.2736118514208741
2211	20	-0.4588950170307413
2211	21	-0.3849201385969394
2211	22	-0.46480348360179846
2211	23	0.11129310944838977
2211	24	-0.042591103733656155
2211	25	-0.03442945280884402
2212	1	-0.12390422256590483
2212	2	-0.22169676076370948
2212	3	-0.14442601880325512
2212	4	-0.2677904798445532
2212	5	-0.21877312914645586
2212	6	-0.2835623888788277
2212	7	-0.3562621535175765
2212	8	-0.23500780728914705
2212	9	-0.18570541411092734
2212	10	-0.24969650928775877
2212	11	-0.38270162489100557
2212	12	-0.22538173619520308
2212	13	-0.33618079763834174
2212	14	-0.3640903403652729
2212	15	-0.5103332738948083
2212	16	-0.2591850124372215
2212	17	-0.22063883176443255
2212	18	-2.911089499858207
2212	19	-1.3450404228494435
2212	20	-0.7224741971553286
2212	21	-0.7430343961801711
2212	22	-0.5077017888092499
2212	23	-0.44946970750429216
2212	24	-0.9278678584856834
2212	25	-0.5074301054079129
2213	1	-0.07724196064555855
2213	2	-0.037326442105246826
2213	3	-0.30987618595506916
2213	4	-0.3168410700182278
2213	5	-0.42260484290443046
2213	6	-0.2759096615721006
2213	7	-0.07964200678966193
2213	8	-0.6121164237624832
2213	9	-0.3749791296183389
2213	10	-0.33959384961050887
2213	11	-0.36566491797908185
2213	12	-0.43428618021025756
2213	13	-0.20966328981056392
2213	14	-0.284625184609709
2213	15	-0.4396776107831073
2213	16	-0.9913785137742224
2213	17	-0.5987702012457703
2213	18	-0.45144642975002114
2213	19	-0.1782033839193815
2213	20	-1.0188970029371802
2213	21	-0.5713038295261796
2213	22	-0.4546817326695063
2213	23	-0.9739622786502977
2213	24	-0.6329373682914365
2213	25	-0.7560743992876056
2214	1	-1.8579206935666868
2214	2	-4.081295278747
2214	3	-2.0867949028945993
2214	4	-1.7448039936202306
2214	5	-1.7560689757441068
2214	6	-1.8949227417106655
2214	7	-1.8264184849039502
2214	8	-1.6489688088144305
2214	9	-1.7681591569095794
2214	10	-1.8899099390209955
2214	11	-1.580781807822408
2214	12	-2.005356355085059
2214	13	-2.0137657524307535
2214	14	-3.2416524726567175
2214	15	-3.443633919989381
2214	16	-2.482165605441562
2214	17	-3.121673457909168
2214	18	-2.300404854112106
2214	19	-4.378664903212027
2214	20	-2.821209241279437
2214	21	-2.642124808789763
2214	22	-2.8701847817450705
2214	23	-3.0518443640229087
2214	24	-3.229715565968206
2214	25	-3.0444271730734265
2215	1	-0.6548469707205342
2215	2	-2.3564923527954598
2215	3	-1.049687177711391
2215	4	-0.6932876698198385
2215	5	-0.6253828797395578
2215	6	-1.2897650849613065
2215	7	-1.5989381997770238
2215	8	-1.6725272793017396
2215	9	-1.2306831485869907
2215	10	-2.645967790890218
2215	11	-1.6202885264878475
2215	12	-1.8644853906034484
2215	13	-1.2021987641822562
2215	14	-2.58404894685723
2215	15	-2.5804011438355268
2215	16	-2.189677496102746
2215	17	-2.2360521338324517
2215	18	-1.1531454691653593
2215	19	-1.8521268593197675
2215	20	-1.962514159504849
2215	21	-1.521849121700484
2215	22	-1.3207600094388496
2215	23	-1.7924267562352405
2215	24	-1.57260394734962
2215	25	-1.5896273190523615
2216	1	-1.2775906431679402
2216	2	-1.277960148931669
2216	3	-1.8312891023737805
2216	4	-2.347931624956047
2216	5	-1.5351799191408162
2216	6	-1.5747899708974167
2216	7	-1.5126501336159492
2216	8	-2.0147713366494813
2216	9	-2.2669303140652026
2216	10	-1.75760978054371
2216	11	-2.310549664089315
2216	12	-2.7469688093541187
2216	13	-3.076815430768569
2216	14	-2.43533695676982
2216	15	-2.4281409994215615
2216	16	-1.6149915635269991
2216	17	-1.7724833122060346
2216	18	-1.7676389355022515
2216	19	-2.0348868598983216
2216	20	-1.9264794525835498
2216	21	-2.224074150989871
2216	22	-2.668074465781185
2216	23	-2.57182116899304
2216	24	-2.576195171943386
2216	25	-2.654680457798083
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	121298.0
3	1.0
4	1031.0
5	5695.0
6	6832.0
7	6156.0
8	5450.0
9	5321.0
10	4904.0
11	4630.0
12	4550.0
13	4416.0
14	4390.0
15	4368.0
16	4537.0
17	4943.0
18	5351.0
19	6585.0
20	8176.0
21	10567.0
22	13243.0
23	15807.0
24	18564.0
25	21113.0
26	23215.0
27	25940.0
28	29352.0
29	34517.0
30	42157.0
31	52768.0
32	66347.0
33	84492.0
34	110015.0
35	143960.0
36	196931.0
37	312895.0
38	734373.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90496356000608	18.060854204929637	12.315992653510055	25.718189581554228
2	15.545087357629917	20.1156217643163	39.613397013143285	24.725893864910496
3	18.78432321785108	24.470008707457865	29.34417964506965	27.401488429621402
4	12.714982099982649	16.49917076000918	37.37792262789791	33.40792451211026
5	13.860477785390692	37.392660599239655	35.0265879421665	13.72027367320315
6	32.42335333473847	36.45377325233293	17.88594722538792	13.236926187540684
7	28.306321085221363	31.38355837970344	22.03335849823807	18.27676203683713
8	26.009184002283174	35.241125527599806	20.396655258557132	18.353035211559888
9	26.721048959918825	15.115451622049125	19.42518540901901	38.73831400901304
10	15.21561118826074	28.2765560779347	34.03478354944037	22.473049184364193
11	34.89802913053717	22.318552006658944	23.821896632524826	18.961522230279066
12	23.21861163506982	24.518889326708525	30.969402624897118	21.293096413324538
13	28.857620765664876	21.403213764937888	25.56117778669998	24.177987682697253
14	22.012674526570663	20.84856556985237	27.070826343876163	30.067933559700805
15	23.825137937193634	28.883588983305764	23.322869802492498	23.968403277008104
16	23.63950495234187	27.516088116244802	25.6935610229244	23.15084590848893
17	22.822891581996675	27.50890224043604	26.712177369562824	22.95602880800446
18	22.751503742029783	27.282960172594912	27.53561776741968	22.42991831795563
19	23.86700143726269	26.77818121448231	26.872801785526086	22.482015562728915
20	24.047649581119238	26.6087329585596	26.9548429517745	22.38877450854666
21	24.056206058412815	26.175848425890862	26.63692613616226	23.131019379534063
22	23.998446234683847	26.51720613224352	26.544159204054342	22.940188429018292
23	23.7270343900071	26.400880058869557	26.520353926176156	23.35173162494719
24	23.495365566934066	26.46073124111969	26.856116439751904	23.187786752194345
25	23.644998360852206	26.36421164452457	26.676465801501305	23.314324193121923
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	268.0
1	268.0
2	460.0
3	652.0
4	652.0
5	652.0
6	1210.0
7	1768.0
8	1768.0
9	1768.0
10	2694.0
11	3620.0
12	3620.0
13	3620.0
14	6152.0
15	8684.0
16	8684.0
17	8684.0
18	15384.5
19	22085.0
20	22085.0
21	22085.0
22	34174.5
23	46264.0
24	46264.0
25	46264.0
26	64764.0
27	83264.0
28	83264.0
29	83264.0
30	106665.0
31	130066.0
32	130066.0
33	130066.0
34	152391.5
35	174717.0
36	174717.0
37	174717.0
38	197494.0
39	220271.0
40	220271.0
41	220271.0
42	237862.5
43	255454.0
44	255454.0
45	255454.0
46	266627.5
47	277801.0
48	277801.0
49	277801.0
50	275822.5
51	273844.0
52	273844.0
53	273844.0
54	254278.0
55	234712.0
56	234712.0
57	234712.0
58	205857.0
59	177002.0
60	177002.0
61	177002.0
62	146954.5
63	116907.0
64	116907.0
65	116907.0
66	91343.0
67	65779.0
68	65779.0
69	65779.0
70	48821.0
71	31863.0
72	31863.0
73	31863.0
74	22356.0
75	12849.0
76	12849.0
77	12849.0
78	8834.5
79	4820.0
80	4820.0
81	4820.0
82	3191.5
83	1563.0
84	1563.0
85	1563.0
86	1019.0
87	475.0
88	475.0
89	475.0
90	294.5
91	114.0
92	114.0
93	114.0
94	68.5
95	23.0
96	23.0
97	23.0
98	24.0
99	25.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3643543491740835
2	0.03869662313685084
3	0.03501345057322287
4	0.03533980763582282
5	0.0407013879499648
6	0.01743679163034002
7	0.017203679442768627
8	0.023730820694767563
9	0.020607117381310932
10	0.019954403256111038
11	0.01888208719328264
12	0.0198145359435682
13	0.01990778081859676
14	0.02746061569590981
15	0.028766043946309605
16	0.020980096881425155
17	0.022658504631939167
18	0.09646182321704143
19	0.02498962650765307
20	0.02261188219442489
21	0.026388299633081418
22	0.019674668631025367
23	0.022751749506967723
24	0.018602352568196968
25	0.022518637319396333
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2144890.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.75343907530583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.89758473012404	61.13981964883207
2	11.330892206699996	16.713845360714117
3	2.9999336415233993	6.637662691801694
4	1.151277499486174	3.3964269966849594
5	0.5590217282630058	2.0614887488608886
6	0.28805825820709724	1.2747172318089517
7	0.18709232270742246	0.9659091556981538
8	0.1256663935333987	0.7414662959423143
9	0.08276885738039094	0.5494039092123606
>10	0.3600678419441572	4.289648662258683
>50	0.01035065904821062	0.5143512720983406
>100	0.006146672673794399	0.8507972231681595
>500	8.860354292249701E-4	0.4675194078855853
>1k	2.5315297977854484E-4	0.3969433950338253
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3356	0.15646490029791738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.6622437514278125E-5	0.0	0.0	0.0
3	0.0	4.6622437514278125E-5	0.0	0.0	0.0
4	0.0	4.6622437514278125E-5	0.0	0.0	0.0
5	0.0	4.6622437514278125E-5	0.0	0.0	0.0
6	0.0	4.6622437514278125E-5	0.0	4.6622437514278125E-5	0.0
7	0.0	4.6622437514278125E-5	0.0	4.6622437514278125E-5	0.0
8	0.0	4.6622437514278125E-5	0.0	4.6622437514278125E-5	0.0
9	0.0	4.6622437514278125E-5	0.0	9.324487502855625E-5	4.6622437514278125E-5
10	0.0	4.6622437514278125E-5	0.0	9.324487502855625E-5	4.6622437514278125E-5
11	0.0	4.6622437514278125E-5	0.0	9.324487502855625E-5	4.6622437514278125E-5
12	0.0	4.6622437514278125E-5	0.0	9.324487502855625E-5	9.324487502855625E-5
13	0.0	4.6622437514278125E-5	0.0	9.324487502855625E-5	1.864897500571125E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	45	6.7865744E-4	14.77123	10
GTGCGCC	75	1.485957E-5	12.661055	11
ACCGAGT	70	1.09603534E-4	12.20859	8
GGTATCA	945	0.0	11.191102	1
TAAGGCG	70	0.0014955809	10.85385	5
ACCGTCC	80	3.7882262E-4	10.682515	8
GTATCAA	2010	0.0	10.617778	1
TAAGACA	295	0.0	10.301961	4
GTAGGAC	675	0.0	10.270959	3
TTAGGAC	420	0.0	10.175486	3
GTCTAAT	95	1.5971018E-4	10.029011	1
TAGGACG	550	0.0	10.015144	4
GTAAGAC	190	1.0004442E-10	9.996968	3
TAGGACA	355	0.0	9.630882	4
GTCCTAC	715	0.0	9.594186	1
TAGGACC	350	0.0	9.49712	4
GTCCTAA	395	0.0	9.406958	1
GGACGTG	535	0.0	9.406607	6
GTCCTAG	265	0.0	9.347795	1
GTTCTAA	195	1.5970727E-9	9.283263	1
>>END_MODULE